From a07c30c2c3e503cfbe12800b2764724d74703c2a Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Wed, 6 Nov 2024 15:41:26 +0100 Subject: [PATCH] added plant material and growth conditions --- .../plant_material_growth_conditions.md | 24 ++++++++++++++ ...iurnal_plant_material_growth_conditions.md | 2 +- ...iurnal_plant_material_growth_conditions.md | 32 +++++++++++++++++++ .../plant_material_growth_conditions.md | 28 ++++++++++++++++ 4 files changed, 85 insertions(+), 1 deletion(-) create mode 100644 studies/biparental_mapping_eam7/protocols/plant_material_growth_conditions.md create mode 100644 studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md create mode 100644 studies/generating_lwd1_mutants/protocols/plant_material_growth_conditions.md diff --git a/studies/biparental_mapping_eam7/protocols/plant_material_growth_conditions.md b/studies/biparental_mapping_eam7/protocols/plant_material_growth_conditions.md new file mode 100644 index 0000000..7834c20 --- /dev/null +++ b/studies/biparental_mapping_eam7/protocols/plant_material_growth_conditions.md @@ -0,0 +1,24 @@ +**Plant material and growth conditions** + +**plant material** + +species: Hordeum vulgare + +genotypes: +- BW: Bowman, spring cultivar +- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) +- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) +- BW(Ppd-H1, eam7): cross of the two introgression lines + + +**growth condtions** + +- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) +- QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3) +- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) + + +**References** + +Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110. +166249 diff --git a/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md b/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md index 38b6565..25d595a 100644 --- a/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md +++ b/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md @@ -21,7 +21,7 @@ genotypes: **sampling** - 2 weeks old plants -- youngest, fully elongated leaf: leaf +- youngest, fully elongated leaf: leaf 2 - sample every 2 hours over 24 hours: ZT0 (lights are turned on) ZT2 diff --git a/studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md b/studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md new file mode 100644 index 0000000..2edaabf --- /dev/null +++ b/studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md @@ -0,0 +1,32 @@ +**eam7 diurnal expression: plant material and growth conditions** + +**plant material* + +species: Hordeum vulgare +genotypes: + +- GP-fast: WT, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) +- lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa +- lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa +- lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa + + + +**growth condtions** + +- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) +- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) +- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) + + +**sampling** + +- 2 weeks old plants +- youngest, fully elongated leaf: leaf 2 +- sample every 2 hours over 24 hours: + ZT0 (lights are turned on) + ZT4 + ZT8 (lights are turned off) + ZT12 + ZT16 + ZT20 \ No newline at end of file diff --git a/studies/generating_lwd1_mutants/protocols/plant_material_growth_conditions.md b/studies/generating_lwd1_mutants/protocols/plant_material_growth_conditions.md new file mode 100644 index 0000000..a74a7bc --- /dev/null +++ b/studies/generating_lwd1_mutants/protocols/plant_material_growth_conditions.md @@ -0,0 +1,28 @@ +**Plant material and growth conditions** + +**plant material** + +species: Hordeum vulgare + + +- GP-fast: Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) + +**growth condtions** + +parents: +- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) +- 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3) +- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) + +- F1 crosses: +- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) +- 7x7x7.5 cm pots (pot volume: 367.5 cm^3) +- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) + + +**References** + +Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110. +166249 + +Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261 -- GitLab