diff --git a/assays/biparental_mapping/protocols/CAPS_marker_analysis.md b/assays/biparental_mapping/protocols/CAPS_marker_analysis.md index 998432dadcc63a0bc9cc9b00777966137c279c61..cf942a5f21fd0baf48feca5f371ac0c4c0de3aec 100644 --- a/assays/biparental_mapping/protocols/CAPS_marker_analysis.md +++ b/assays/biparental_mapping/protocols/CAPS_marker_analysis.md @@ -1,9 +1,9 @@ -**CAPS marker: PCR + digest** +## CAPS marker: PCR + digest -PCR: +**PCR:** - using GoTaq® DNA Polymerase (Promega) according to the manufacturer's protocol with CAPS-marker specific PCR primers -digest: +**Digest:** - digesting 5µl of PCR fragments with the CAPS-marker specific restriction enzyme: