From a69750a3bc16981905ae807a643235aeefa2bf97 Mon Sep 17 00:00:00 2001 From: Gesa Helmsorig <gesa.helmsorig@hhu.de> Date: Wed, 13 Nov 2024 12:47:49 +0000 Subject: [PATCH] Update file CAPS_marker_analysis.md --- assays/biparental_mapping/protocols/CAPS_marker_analysis.md | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/assays/biparental_mapping/protocols/CAPS_marker_analysis.md b/assays/biparental_mapping/protocols/CAPS_marker_analysis.md index 998432d..cf942a5 100644 --- a/assays/biparental_mapping/protocols/CAPS_marker_analysis.md +++ b/assays/biparental_mapping/protocols/CAPS_marker_analysis.md @@ -1,9 +1,9 @@ -**CAPS marker: PCR + digest** +## CAPS marker: PCR + digest -PCR: +**PCR:** - using GoTaq® DNA Polymerase (Promega) according to the manufacturer's protocol with CAPS-marker specific PCR primers -digest: +**Digest:** - digesting 5µl of PCR fragments with the CAPS-marker specific restriction enzyme: -- GitLab