diff --git a/assays/embryo_transformation/dataset/transformation_T0_results.xlsx b/assays/embryo_transformation/dataset/transformation_T0_results.xlsx index 1c5ce99ef745e7d374de09a5af1a21a9792f9ee7..2303b9d8dfc780f44d8d6eee113c3199c3a1a513 100644 Binary files a/assays/embryo_transformation/dataset/transformation_T0_results.xlsx and b/assays/embryo_transformation/dataset/transformation_T0_results.xlsx differ diff --git a/assays/embryo_transformation/isa.assay.xlsx b/assays/embryo_transformation/isa.assay.xlsx index 645d4fabd14c575c032264b2209b93aaaf25c475..dbc1037251e06aef9256ca6891e195ca96bc3c69 100644 Binary files a/assays/embryo_transformation/isa.assay.xlsx and b/assays/embryo_transformation/isa.assay.xlsx differ diff --git a/assays/embryo_transformation/protocols/cloning_trafo_protocol.md b/assays/embryo_transformation/protocols/cloning_trafo_protocol.md index c4832045654e3ce22464851457ec698a7affe7b1..911abf527d2700c078acbfe8b8a305fb2631c552 100644 --- a/assays/embryo_transformation/protocols/cloning_trafo_protocol.md +++ b/assays/embryo_transformation/protocols/cloning_trafo_protocol.md @@ -15,9 +15,6 @@ - GP-fast plants were cultivated in long-day conditions (16h day / 8 night) in control temperature (20°C / 16°C) - immature embryos were used for transformation according to Hensel et al. (2009) -- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol -- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3') - **References:** diff --git a/assays/embryo_transformation/protocols/test_hygromycin.md b/assays/embryo_transformation/protocols/test_hygromycin.md new file mode 100644 index 0000000000000000000000000000000000000000..d9a546bfb2c6e58dc51fe292daaeb985d12e31e5 --- /dev/null +++ b/assays/embryo_transformation/protocols/test_hygromycin.md @@ -0,0 +1,5 @@ +## Test regenerated plants for successful transformation + +- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol +- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3') + diff --git a/studies/generating_lwd1_mutants/isa.study.xlsx b/studies/generating_lwd1_mutants/isa.study.xlsx index c94be8f49e715440d9ad6ea1af477a2582fd5967..b73dc2b9b607e8f7aa8443b26ae46d7506742150 100644 Binary files a/studies/generating_lwd1_mutants/isa.study.xlsx and b/studies/generating_lwd1_mutants/isa.study.xlsx differ