diff --git a/studies/MSA_dissection_eam7/protocols/plant_material_growth_conditions.md b/studies/MSA_dissection_eam7/protocols/plant_material_growth_conditions.md index 1d877c79c3c33afb73277e4ccb09ce6a02993005..ff4d7acfc1eddbd0d56da18ffcd57b18a740ec7a 100644 --- a/studies/MSA_dissection_eam7/protocols/plant_material_growth_conditions.md +++ b/studies/MSA_dissection_eam7/protocols/plant_material_growth_conditions.md @@ -1,6 +1,6 @@ -**Plant material and growth conditions** +## Plant material and growth conditions -**plant material** +**Plant material:** species: Hordeum vulgare @@ -10,20 +10,13 @@ genotypes: - BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) - BW(Ppd-H1, eam7): cross of the two introgression lines -**growth condtions** +**Growth condtions:** - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - - QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - -**plants** -- SD: plant 1 - 204 -- LD: plant 205 - 342 - - **References** Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249