diff --git a/assays/plant_genotyping/protocols/complementation_plant_genotyping.md b/assays/plant_genotyping/protocols/complementation_plant_genotyping.md index 54ea91ec91beb89bc95ccfea5117d0c158ac8be6..69e5f298c8df764d5f5f45e94a514a29ce33898c 100644 --- a/assays/plant_genotyping/protocols/complementation_plant_genotyping.md +++ b/assays/plant_genotyping/protocols/complementation_plant_genotyping.md @@ -1,4 +1,4 @@ -**F1 plants of lwd/eam7 allelism test: genoypting ** +## F1 plants of *lwd/eam7* allelism test: genotyping - DNA was extracted from leaves of all plants using QIAGEN_DNeasy-Plant_mini protocol - plants were tested for possible mutations in the CDS of LWD1 by amplifying the full genomic sequence using the primers LWD1_71us_F (5'-ATCAGACCCCTCCAACGACT-3') and LWD1_21ds_R (5'-TTCCTCTTTCTACCCCACCA-3')