diff --git a/studies/generating_lwd1_mutants/README.md b/studies/generating_lwd1_mutants/README.md
index 2cb4fbb6af8359e4d4dfdf013f5f5289784a04ca..e66b9ca363274b357521251af88aeedfe47022bc 100644
--- a/studies/generating_lwd1_mutants/README.md
+++ b/studies/generating_lwd1_mutants/README.md
@@ -1,7 +1,21 @@
-Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence.
+**Generating *lwd1* mutants**
+
+Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence. The resulting T0 plants were genotyped to screen for different induced mutations. Promising T1 plants were sown, and genotyped again. From this, three different homozygous mutants were selected for gene expression analysis and phenotyping: *lwd1-26*, *lwd1-390* and *lwd1-402*.
 
 **study overview**
 
 	WT transformation plants (GP-fast)
 	-> assay: embryo transformation
-		output: T0 mutant plants (used in study "genotyping_lwd1_mutants"
\ No newline at end of file
+		output: *T0 mutant plants*
+		
+			-> assay: plant_genotyping
+				output: T0 mutant genotype info
+
+			-> assay: plant propagation
+				output: *T1 mutant plants*
+
+				-> assay: plant_genotyping
+					output: T1 mutant genotype info
+
+				-> assay: plant propagation
+					output: T2 mutant plants (used in studies gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants)
\ No newline at end of file