From c9ba0fe0ed4d584d73b36b27575cc2697b58d673 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Tue, 5 Nov 2024 14:46:38 +0000
Subject: [PATCH] Update README.md

---
 studies/generating_lwd1_mutants/README.md | 18 ++++++++++++++++--
 1 file changed, 16 insertions(+), 2 deletions(-)

diff --git a/studies/generating_lwd1_mutants/README.md b/studies/generating_lwd1_mutants/README.md
index 2cb4fbb..e66b9ca 100644
--- a/studies/generating_lwd1_mutants/README.md
+++ b/studies/generating_lwd1_mutants/README.md
@@ -1,7 +1,21 @@
-Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence.
+**Generating *lwd1* mutants**
+
+Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence. The resulting T0 plants were genotyped to screen for different induced mutations. Promising T1 plants were sown, and genotyped again. From this, three different homozygous mutants were selected for gene expression analysis and phenotyping: *lwd1-26*, *lwd1-390* and *lwd1-402*.
 
 **study overview**
 
 	WT transformation plants (GP-fast)
 	-> assay: embryo transformation
-		output: T0 mutant plants (used in study "genotyping_lwd1_mutants"
\ No newline at end of file
+		output: *T0 mutant plants*
+		
+			-> assay: plant_genotyping
+				output: T0 mutant genotype info
+
+			-> assay: plant propagation
+				output: *T1 mutant plants*
+
+				-> assay: plant_genotyping
+					output: T1 mutant genotype info
+
+				-> assay: plant propagation
+					output: T2 mutant plants (used in studies gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants)
\ No newline at end of file
-- 
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