diff --git a/studies/allelism_test/README.md b/studies/allelism_test/README.md index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..5543c14c4c86457be551c67ee41bf23dd37a8e15 100644 --- a/studies/allelism_test/README.md +++ b/studies/allelism_test/README.md @@ -0,0 +1,16 @@ +**Allelism test* + +Complementation crosses were performed to confirm that *lwd1* and *eam7* are allelic, which are both recessive mutations. For this, homozygous *lwd1* mutants *lwd1-26*, *lwd1-390*, and *lwd1-402* were crossed with BW(Ppd-H1, eam7). F1 plants were grown under SD conditions and flowering time was scored. If *lwd1* and *eam7* are allelic, we expected the F1 plants to display the mutant phenotype and be early flowering under SD. + +**study overview** + + parent plants (*lwd1* mutants, BW(Ppd-H1,eam7), introgression line background BW(Ppd-H1), mutant background GP-fast) + + -> assay: complementation_crosses + output: F1 plants + + -> assay: plant_genotyping + output: F1 genotype information + + -> assay: plant_phenotyping + output: F1 flowering data \ No newline at end of file