diff --git a/studies/gene_expression_lwd1_mutants_diurnal/README.md b/studies/gene_expression_lwd1_mutants_diurnal/README.md index f2ef84c9da96feb7a1afeffa4c138774bf368227..a3cfcbf57df3df496831a1db2d04c7faba7079df 100644 --- a/studies/gene_expression_lwd1_mutants_diurnal/README.md +++ b/studies/gene_expression_lwd1_mutants_diurnal/README.md @@ -1,14 +1,14 @@ -**Test diurnal gene expression of circadian clock and flowering time genes in *lwd1* mutants and WT plants** +## Test diurnal gene expression of circadian clock and flowering time genes in *lwd1* mutants and WT plants 2-week old plants from the genotypes *lwd1-26*, *lwd1-390*, *lwd1-402*, and GP-fast were sampled at Zeitgeber Time (ZT) 0, 4, 8, 12, 16, and 20. Gene expression of CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1, and PRR1 were tested. -**study overview** +**Study overview:** -input: input: 2-weeks old lwd1-26, lwd1-390, lwd1-402 and GP-fast plants grown under SD. + input: 2-weeks old lwd1-26, lwd1-390, lwd1-402 and GP-fast plants grown under SD. --> assay: leaf_sampling + -> assay: leaf_sampling output: frozen leaf tissue (3 biol. replicates) -> assay: RNA_extraction