diff --git a/assays/qRT-PCR/protocols/qRT-PCR_primer.md b/assays/qRT-PCR/protocols/qRT-PCR_primer.md new file mode 100644 index 0000000000000000000000000000000000000000..0b3ccec975b1ae64855298742d0aed31fb00cc35 --- /dev/null +++ b/assays/qRT-PCR/protocols/qRT-PCR_primer.md @@ -0,0 +1,44 @@ + + +| Target gene | Primer | Sequence 5’-3’ | Fragment size (bp) | +| - | - | - | - | +| ACT (HORVU.MOREX.r3.5HG0457850) | ACT_591F | CGTGTTGGATTCTGGTGATG | 208| +| | ACT_789R | AGCCACATATGCGAGCTTCT | | +| CO1 (HORVU.MOREX.r3.7HG0671540) | CO1_2185F | CTGCTGGGGCTAGTGCTTAC | 251| +| | CO1_3454R | CCTTGTTGCATAACGTGTGG | | +| CO2 (HORVU.MOREX.r3.6HG0611630) | CO2_564F | AGTGGACTCTTGGCTCCTCA | 158 | +| | CO2_721R | CATGCTGCTGTTCTTGCATT | | +| ELF3 (HORVU.MOREX.r3.1HG0095050) | ELF3_DL_3060_F3 | TGCTGTCCAAGTGTTTGAGC | 242 | + | | Elf3_DL_4483_R3 | CCTGGTTTCCTTCGGTGTTA | | +| FT1 (HORVU.MOREX.r3.7HG0653910) | FT1_1955F | GGTAGACCCAGATGCTCCAA | 120 | +| | FT1_2183R | TCGTAGCACATCACCTCCTG | | +| FT2 (HORVU.MOREX.r3.3HG0244930 | FT2_4031R | TCACATCCTTCTCCCGCCGG | 238 | +| | FT2_3809_F | TACCGAGGTTGTGTGCTACG | | +| FT3 (HORVU.MOREX.r3.1HG0077250) | FT3-1004F | TGTCAGACATCCCTGGAACA | 225 | +| | FT3-1848R | CTGATCCACCTTCCCTTTGA | | +| FT4 (HORVU.MOREX.r3.2HG0117260) | FT4_626F | AAACGATAGCTTTGGAAAAGAGATC | 230 | +| | FT4_2537R | TCCTCCCGCCGGTGCCCGTG | | +| GI (HORVU.MOREX.r3.3HG0238250) | GI_6780F | TCAGTTAGAGCTCCTGGAAGT | 263 | +| | GI_7289R | GGTAGTTTGGGCTTTGGATG | | +| LHY (HORVU.MOREX.r3.7HG0699010) | HvLHY_672F | CCTGGAATTGGAGATGGAGA | 210 | +| | HvLHY_882R | TGAGCATGGCTTCTGATTTG | | +| LUX1 (HORVU.MOREX.r3.3HG0328340) | LUX_1077F | AATTCAGTCCACGGATGCTC | 222 | +| | LUX_1298R | CTTCACTTCAGCTCCCCTTG | | +| LWD1 (HORVU.MOREX.r3.6HG0583670) | LWD1_qPCR-114_F | CAGCTCATAGCCCATGACAA | 114 | +| | LWD1_qPCR-114_R | CTCCTTGTCCCGAAGATCAA | | +| PHYB (HORVU.MOREX.r3.4HG0381880) | HvPHYB-1151F | CTTGCGCACCAACTATCAGA | 232 | +| | HvPHYB-1383R | CTCCATGACACACCGTCAAC | | +| PHYC (HORVU.MOREX.r3.5HG0511090) | PHYC_0986F | ACTACCCGGCAACTGACATC | 142 | +| | PHYC_1127R | GAGCCACAGAGGCTGATAGG | | +| Ppd-H1 (HORVU.MOREX.r3.2HG0107710) | PPD1_2165F | GATGGATTCAAAGGCAAGGA | 172 | +| | PPD1_2336R | GAACAATTGGCTCCTCCAAA | | +| PRR1 (HORVU.MOREX.r3.6HG0595250) | HvPRR1_1056F | GAGCATAGCATGGCACTTCA | 237 | +| | HvPRR1_1292R | TGTCTTTCCTCGGAAATTGG | | +| PRR59 (HORVU.MOREX.r3.4HG0350690) | PRR59_2064_F | GAAATTCCGCATGAAAAGGA | 148 | +| | PRR59_2212_R | TTCCGCATCTTCTGTTGTTG | | +| PRR73 (HORVU.MOREX.r3.5HG0498830) | PRR73_441F | GCGCCGTAGAGAATCAGAAC | 222 | +| | PRR73_662R | CATGTCGGGTACAGTCATCG | | +| PRR95 (HORVU.MOREX.r3.5HG0498830) | PRR95_1467F | CAGAACTCCAGTGTCGCAAA | 251 | +| | PRR95_1717R | TGCTGTTGCCAGAGTTGTTC | | +| VRN1 (HORVU.MOREX.r3.5HG0511210) | HVBM5a_292F | CTGAAGGCGAAGGTTGAGAC | 203 | +| | HVBM5a_494R | TTCTCCTCCTGCAGTGACCT | | \ No newline at end of file diff --git a/assays/qRT-PCR/protocols/qRT-PCR_primer.txt b/assays/qRT-PCR/protocols/qRT-PCR_primer.txt deleted file mode 100644 index 142186ea98a706bccc08b68d4b5635f41ccf4dc8..0000000000000000000000000000000000000000 --- a/assays/qRT-PCR/protocols/qRT-PCR_primer.txt +++ /dev/null @@ -1,41 +0,0 @@ -Target gene Primer Sequence 5’-3’ Fragment size (bp) -ACT (HORVU.MOREX.r3.5HG0457850) ACT_591F CGTGTTGGATTCTGGTGATG 208 - ACT_789R AGCCACATATGCGAGCTTCT -CO1 (HORVU.MOREX.r3.7HG0671540) CO1_2185F CTGCTGGGGCTAGTGCTTAC 251 - CO1_3454R CCTTGTTGCATAACGTGTGG -CO2 (HORVU.MOREX.r3.6HG0611630) CO2_564F AGTGGACTCTTGGCTCCTCA 158 - CO2_721R CATGCTGCTGTTCTTGCATT -ELF3 (HORVU.MOREX.r3.1HG0095050) ELF3_DL_3060_F3 TGCTGTCCAAGTGTTTGAGC 242 - Elf3_DL_4483_R3 CCTGGTTTCCTTCGGTGTTA -FT1 (HORVU.MOREX.r3.7HG0653910) FT1_1955F GGTAGACCCAGATGCTCCAA 120 - FT1_2183R TCGTAGCACATCACCTCCTG -FT2 (HORVU.MOREX.r3.3HG0244930 FT2_4031R TCACATCCTTCTCCCGCCGG 238 - FT2_3809_F TACCGAGGTTGTGTGCTACG -FT3 (HORVU.MOREX.r3.1HG0077250) FT3-1004F TGTCAGACATCCCTGGAACA 225 - FT3-1848R CTGATCCACCTTCCCTTTGA -FT4 (HORVU.MOREX.r3.2HG0117260) FT4_626F AAACGATAGCTTTGGAAAAGAGATC 230 - FT4_2537R TCCTCCCGCCGGTGCCCGTG -GI (HORVU.MOREX.r3.3HG0238250) GI_6780F TCAGTTAGAGCTCCTGGAAGT 263 - GI_7289R GGTAGTTTGGGCTTTGGATG -LHY (HORVU.MOREX.r3.7HG0699010) HvLHY_672F CCTGGAATTGGAGATGGAGA 210 - HvLHY_882R TGAGCATGGCTTCTGATTTG -LUX1 (HORVU.MOREX.r3.3HG0328340) LUX_1077F AATTCAGTCCACGGATGCTC 222 - LUX_1298R CTTCACTTCAGCTCCCCTTG -LWD1 (HORVU.MOREX.r3.6HG0583670) LWD1_qPCR-114_F CAGCTCATAGCCCATGACAA 114 - LWD1_qPCR-114_R CTCCTTGTCCCGAAGATCAA -PHYB (HORVU.MOREX.r3.4HG0381880) HvPHYB-1151F CTTGCGCACCAACTATCAGA 232 - HvPHYB-1383R CTCCATGACACACCGTCAAC -PHYC (HORVU.MOREX.r3.5HG0511090) PHYC_0986F ACTACCCGGCAACTGACATC 142 - PHYC_1127R GAGCCACAGAGGCTGATAGG -Ppd-H1 (HORVU.MOREX.r3.2HG0107710) PPD1_2165F GATGGATTCAAAGGCAAGGA 172 - PPD1_2336R GAACAATTGGCTCCTCCAAA -PRR1 (HORVU.MOREX.r3.6HG0595250) HvPRR1_1056F GAGCATAGCATGGCACTTCA 237 - HvPRR1_1292R TGTCTTTCCTCGGAAATTGG -PRR59 (HORVU.MOREX.r3.4HG0350690) PRR59_2064_F GAAATTCCGCATGAAAAGGA 148 - PRR59_2212_R TTCCGCATCTTCTGTTGTTG -PRR73 (HORVU.MOREX.r3.5HG0498830) PRR73_441F GCGCCGTAGAGAATCAGAAC 222 - PRR73_662R CATGTCGGGTACAGTCATCG -PRR95 (HORVU.MOREX.r3.5HG0498830) PRR95_1467F CAGAACTCCAGTGTCGCAAA 251 - PRR95_1717R TGCTGTTGCCAGAGTTGTTC -VRN1 (HORVU.MOREX.r3.5HG0511210) HVBM5a_292F CTGAAGGCGAAGGTTGAGAC 203 - HVBM5a_494R TTCTCCTCCTGCAGTGACCT \ No newline at end of file