From edc7a4e0e41d43d21d82761b0c8188c729d6ee4e Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Wed, 13 Nov 2024 12:24:06 +0000
Subject: [PATCH] Update file diurnal_plant_material_growth_conditions.md

---
 ...iurnal_plant_material_growth_conditions.md | 29 ++++++++++---------
 1 file changed, 15 insertions(+), 14 deletions(-)

diff --git a/studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md b/studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md
index 43e9ec0..d2ab619 100644
--- a/studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md
+++ b/studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md
@@ -1,37 +1,38 @@
-**lwd1 mutants diurnal expression: plant material and growth conditions**
+## *lwd1* mutants diurnal expression: plant material and growth conditions
 
-**plant material*
+**Plant material:**
 
-species: Hordeum vulgare
-genotypes:
+Species: *Hordeum vulgare*
 
-- GP-fast: WT, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) 
+Genotypes:
+
+- GP-fast: wild-type, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) 
 - lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa
 - lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa
 - lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa
 
 
 
-**growth condtions**
+**Growth condtions:**
 
 - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
 - QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
 - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
 
 
-**sampling**
+**Sampling:**
 
 - 2 weeks old plants
 - youngest, fully elongated leaf: leaf 2
 - sample every 2 hours over 24 hours: 
-	ZT0 (lights are turned on)
-	ZT4
-	ZT8 (lights are turned off)
-	ZT12
-	ZT16
-	ZT20
+	- ZT0 (lights are turned on)
+	- ZT4
+	- ZT8 (lights are turned off)
+	- ZT12
+	- ZT16
+	- ZT20
 	
-**References**
+**References:**
 
 Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
 
-- 
GitLab