From f987d9466fa49bd2aa86058e455fd556c0101b01 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Wed, 23 Oct 2024 10:57:41 +0000
Subject: [PATCH] Update CAPS_marker_analysis.md

---
 .../protocols/CAPS_marker_analysis.md         | 35 ++++++++++---------
 1 file changed, 19 insertions(+), 16 deletions(-)

diff --git a/assays/biparental_mapping/protocols/CAPS_marker_analysis.md b/assays/biparental_mapping/protocols/CAPS_marker_analysis.md
index 1d3e55f..a6e402b 100644
--- a/assays/biparental_mapping/protocols/CAPS_marker_analysis.md
+++ b/assays/biparental_mapping/protocols/CAPS_marker_analysis.md
@@ -8,20 +8,23 @@ digest:
 - digesting 5µl of PCR fragments with the CAPS-marker specific restriction enzyme:
 
 
-Marker		Primer	Sequence 5’-3’			Enzyme		annealing temp (°C)		EAM7 allele: fragment size (bp)		eam7 allele: fragment size (bp)
-M-13		F		GTAATTGCCCTAGCGTTGGA	MnlI		58°C					246, 98								328	
-			R		CAGAACCCGGGAATTAGGAT											
-M-16		F		AAAACGGTGGCATTAGCAAG	HindIII		58°C					378									214, 168	
-			R		CGGAGGCATGATCAGGTATT																	
-M-11		F		AAAGGCCCACACTAGTGCAT	BstUI		58°C					209, 128							337	
-			R		ACGCTCACTCCCTGCATCT												
-M-39		F		CTAAATTTCAGCCCCGAACA	DdeI		55°C					237,100								334	
-			R		TGCCTGGACTTGTTCTGATG											
-M-PRR1		F		GAGCATAGCATGGCACTTCA	Hpy188I		58°C					224, 158							383	
-			R		GCCTGCCTAACAAACTGACC											
-lwd1-del	F		TACCATCCTCCCATTGCCTG	-			60°C	 				199									167	
-			R		ATTGGCTTACACTGCAGGGG											
+| Marker |	Primer | Sequence 5’-3’	| Enzyme | annealing temp (°C) | EAM7 allele: fragment size (bp) | eam7 allele: fragment size (bp) | 
+| ------ | ------ | ------ | ------ | ------ | ------ | ------ |
+| M-13 | F | GTAATTGCCCTAGCGTTGGA | MnlI | 58°C | 246, 98 | 328 |
+| | R | CAGAACCCGGGAATTAGGAT | 
+| M-16 | F | AAAACGGTGGCATTAGCAAG | HindIII | 58°C | 378 | 214, 168 | 	
+| |  R | CGGAGGCATGATCAGGTATT | 																	
+| M-11 | F | AAAGGCCCACACTAGTGCAT | BstUI | 58°C | 209, 128 | 337| 	
+| | R | ACGCTCACTCCCTGCATCT | 										
+| M-39 | F | CTAAATTTCAGCCCCGAACA | DdeI | 55°C | 237, 100 | 334	
+| | R | TGCCTGGACTTGTTCTGATG | 											
+| M-PRR1 | F | GAGCATAGCATGGCACTTCA | Hpy188I | 58°C | 224, 158 | 383	
+| | R | GCCTGCCTAACAAACTGACC | 											
+| lwd1-del | F | TACCATCCTCCCATTGCCTG | - | 60°C | 199 | 167 | 	
+| | R |	ATTGGCTTACACTGCAGGGG | 										
 
-Marker		Primer	Sequence 5’-3’			Enzyme		annealing temp (°C)		ppd-H1 allele: fragment size (bp)	Ppd-H1 allele: fragment size (bp)
-M-PPDH11	F		ACGTGAATGGTGGATCGGC		BstUI		60°C					506									433, 73
-			R		TATAGCTAGGTGCGTGGCG						
+
+| Marker |	Primer | Sequence 5’-3’	| Enzyme | annealing temp (°C) | ppd-H1 allele: fragment size (bp) | Ppd-H1 allele: fragment size (bp) | 
+| ------ | ------ | ------ | ------ | ------ | ------ | ------ |
+| M-PPDH11 | F | ACGTGAATGGTGGATCGGC | BstUI | 60°C | 506 |  433, 73 | 
+| | R | TATAGCTAGGTGCGTGGCG | 						
-- 
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