## Leaf sampling for RNA extraction

-	place two 3 mm metal beads into 2.0 ml tube
-	always sample the youngest, fully elongated leaf (auricles are clearly visible)
-	sample three biological replicates for each genotype and time point
-	for each biological replicate: pool the leaf from two plants into one tube
-	freeze samples directly in liquid nitrogen
-	Store samples at -80°C until needed