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HHU Plant Biochemistry / Wrobel-2023-CastorBeanEndospermProteome
Creative Commons Attribution 4.0 InternationalMapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth
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Reversible Burst of Transcriptional Changes during Induction of Crassulacean Acid Metabolism in Talinum triangulare.
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Algae cultures were grown mixotrophically (TAP). After 24h of 35°C/40°C the cells were shifted back to room temperature for 48h. 'omics samples were taken.
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Algae cultures were grown mixotrophically (TAP). After 24h of 35°C/40°C the cells were shifted back to room temperature for 48h. 'omics samples were taken.
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A prototypic ARC that implements all specification standards accordingly
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Script and data of Johan Zicola's paper in Nature Plants. Nice example of how things should be done.
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Algae cultures were grown under mixotrophic (TAP) and phototrophic (HMP) conditions. During 24h of 35°C/40°C heat treatment 'omics samples were taken.
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Differential analysis of lpa2 mutant Chlamy complexome
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MAdLand Project - Schippers Lab
Evolutionary conserved and divergent responses to copper zinc superoxide dismutase inhibition in plants
After initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present-day organisms. Copper-zinc superoxide dismutase (CuZnSOD) evolved during the GOE and are present in charophytes and extant land plants, but nearly absent from chlorophytes. The chemical inhibitor of CuZnSOD, lung cancer screen 1 (LCS-1), could greatly facilitate the study of SODs in diverse plants. Here, we determined the impact of chemical inhibition of plant CuZnSOD activity, on plant growth, transcription and metabolism. We followed a comparative approach by using different plant species, including Marchantia polymorpha and Physcomitrium patens, representing bryophytes, the sister lineage to vascular plants, and Arabidopsis thaliana. We show that LCS-1 causes oxidative stress in plants and that the inhibition of CuZnSODs provoked a similar core response that mainly impacted glutathione homeostasis in all plant species analyzed. That said, Physcomitrium and Arabidopsis, which contain multiple CuZnSOD isoforms showed a more complex and exacerbated response. In addition, an untargeted metabolomics approach revealed a specific metabolic signature for each plant species. Our comparative analysis exposes a conserved core response at the physiological and transcriptional level towards LCS-1, while the metabolic response largely varies. These differences correlate with the number and localization of the CuZnSOD isoforms present in each species.
Plant, Cell & Environment (in submission)
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Natural-Variation-and-Evolution / Microscopy_Collection / map-by-seq_CLSM-stacks
Creative Commons Attribution 4.0 InternationalUpdated