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+# Please add a valid title for your ArcInvestigation
+### Please add a valid description to your ArcInvestigation
+
+## Table of Contents 
+
+
+ 1. Studies 
+
+
+
+ 2. Assays 
+
+     - [RNAseq_raw_data](#assay--rnaseq_raw_data)
+     - [FASTQC](#assay--fastqc)
+
+## Graphical overview
+ 
+```mermaid
+---
+title: Effects_of_Ppd-H1_and_High_Ambient_Temperatures_in_Barley
+---
+flowchart TD
+    classDef investigationStyle fill:#6c7885,color:#2d3e50,fontWeight:bold;
+    classDef studyStyle fill:#62d4c1,color:#2d3e50,fontWeight:bold;
+    classDef assayStyle fill:#ffd34d,color:#2d3e50,fontWeight:bold;
+    classDef processStyle fill:#D46275,color:#2d3e50;
+    id_0["<no-title>"]
+    class id_0 investigationStyle;
+    id_0-->STUDY_id_1
+    class STUDY_id_1 studyStyle;
+    subgraph STUDY_id_1[Study: RNAseq_analysis]
+    end
+    subgraph ASSAY_id_2[Assay: Salmon_quantification]
+    end
+    class ASSAY_id_2 assayStyle;
+    subgraph ASSAY_id_3[Assay: RNAseq_analysis_of_MSA]
+    end
+    class ASSAY_id_3 assayStyle;
+    subgraph ASSAY_id_4[Assay: RNAseq_raw_data]
+        id_5[NewTable0]
+        class id_5 processStyle;
+    end
+    class ASSAY_id_4 assayStyle;
+    subgraph ASSAY_id_6[Assay: RNAseq_analysis_of_leaf]
+    end
+    class ASSAY_id_6 assayStyle;
+    subgraph ASSAY_id_7[Assay: FASTQC]
+        id_5[NewTable0]
+        class id_5 processStyle;
+    end
+    class ASSAY_id_7 assayStyle;
+    id_5-->|1|id_5
+    id_5-->|389|id_8
+    id_8-->|1|id_5
+    id_8-->|1|id_8
+
+```
+ 
+### Additional details
+| Meta Data | Description |
+| --------- | ----------- |
+| Submission Date  | tba |
+| Public Release Date | tba |
+| Study identifiers | RNAseq_analysis |
+| Study Count | 1 |
+| Assay identifiers | Salmon_quantification , RNAseq_analysis_of_MSA , RNAseq_raw_data , RNAseq_analysis_of_leaf , FASTQC |
+| Assay Count | 5 |
+
+
+
+
+## Assay: _RNAseq_raw_data_
+### Additional details
+| Meta Data | Description |
+| --------- | ----------- |
+| Measurement Type | RNA-Seq |
+| Technology Type | Illumina NovaSeq 6000 |
+| Table Count | 1 |
+| Table Names | NewTable0 |
+| Sample Count | 406 |
+| Data File Count | 406 |
+| Associated studies |  |
+### Annotation headers
+
+**Parameters**: [library strategy](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000035),[library preparation kit](http://purl.obolibrary.org/obo/GENEPIO_0000085),[library preparation kit version](http://purl.obolibrary.org/obo/GENEPIO_0000149),[adapter sequence](http://purl.obolibrary.org/obo/GENEPIO_0000083),[next generation sequencing instrument model](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000040),[base-calling software](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000017),[base-calling software version](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000018),[base-calling software parameters](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000019),[library strand](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000020),[Raw data file format](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000021)
+
+## Assay: _FASTQC_
+### Additional details
+| Meta Data | Description |
+| --------- | ----------- |
+| Technology Type | FASTQC |
+| Table Count | 1 |
+| Table Names | NewTable0 |
+| Sample Count | 840 |
+| Data File Count | 0 |
+| Associated studies |  |
+### Annotation headers
+
+**Characteristics**: [Format](https://bioregistry.io/NCIT:C42761)