diff --git a/assays/RNAseq_analysis_of_leaf/isa.assay.xlsx b/assays/RNAseq_analysis_of_leaf/isa.assay.xlsx
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diff --git a/assays/ThreeDRNAseq/README.md b/assays/ThreeDRNAseq/README.md
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+RNAseq analysis was conducted on the leaf and main shoot apex (MSA) samples separately.
+
+The tables of Count and Transcript per Million (TPM) were obtained using ThreeDRNAseq R package.
+The tables of differentially expressed genes were obtained using EdgeR R package.
+Gene Ontology analysis was performed using the Triticeae-Gene Tribe database (http://wheat.cau.edu.cn/TGT/m15/?navbar=GOEnrichment).
+WCGNA analysis was performed using the WGCNA R package.
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