From 7a982aab6fbc1da6b100414dd021d19ec0529941 Mon Sep 17 00:00:00 2001 From: Viktoria Petrova <vipet103@hhu.de> Date: Sun, 9 Mar 2025 14:10:11 +0100 Subject: [PATCH] add protocol to plant samples study --- .../protocols/PlantMaterialsAndGrowthConditions.md | 5 +++++ 1 file changed, 5 insertions(+) create mode 100644 studies/plant-samples/protocols/PlantMaterialsAndGrowthConditions.md diff --git a/studies/plant-samples/protocols/PlantMaterialsAndGrowthConditions.md b/studies/plant-samples/protocols/PlantMaterialsAndGrowthConditions.md new file mode 100644 index 0000000..2845054 --- /dev/null +++ b/studies/plant-samples/protocols/PlantMaterialsAndGrowthConditions.md @@ -0,0 +1,5 @@ +## Plant Materials and Growth Conditions + +In this study, we selected two genotypes carrying a wild-type *Ppd-H1* allele and three genotypes with mutant *ppd-h1* allele to study the interaction between *Ppd-H1* and HT on shoot growth and inflorescence development. Golden Promise (GP) and Scarlett are spring barley cultivars carrying a natural mutation in the CCT domain of *PPD-H1* (G>T at CDS position 1969), according to gene model HORVU.MOREX.r3.2HG0107710.1 (Supplementary Dataset 1), causing a Gly-to-Trp amino acid exchange, and resulting in a late flowering time under long-day (LD; 16 h/8 h, day/night) conditions. GP-fast and S42-IL107 are two near-isogenic lines (NILs) derived from GP and Scarlett, respectively, carrying wild-type Ppd-H1 alleles from winter barley Igri and wild barley (*Hordeum vulgare ssp. spontaneum*) accession ISR42-8 (Supplementary Dataset 1). These lines are early flowering under LD (Schmalenbach et al., 2011; Digel, Pankin and von Korff, 2015; Gol, Haraldsson and Von Korff, 2021). + +Plants were sown in a 96-well growing tray with ED73 soil (Einheitserde Werkverband e.V., Germany) mixed with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M (ICL Group Ltd.). Seeds were stratified for three days at 4 °C in the soil for even germination and then transferred to controlled growth chambers (PAR 300 µM/m2s, humidity 60%) with inductive LD conditions (16 h/8 h, day/night) set to control (CT; 20 °C/16 °C, day/night) or high ambient temperature (HT; 28 °C/24 °C, day/night). \ No newline at end of file -- GitLab