diff --git a/README.md b/README.md
index bc296a9d28c7ad120f3a9cab6d536fd0c6e635bd..ae4b3108a608c3c939c7bee0ab978c0e2e250e7a 100644
--- a/README.md
+++ b/README.md
@@ -14,32 +14,15 @@ High ambient temperature (HT) impairs reproductive development and grain yield i
 
 The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
 
-## Table of Contents 
-
-
- 1. Studies 
-
-     - [plant-samples](#study--plant-samples)
-
- 2. Assays 
-
-     - [Salmon_quantification](#assay--salmon_quantification)
-     - [RNAseq_raw_data](#assay--rnaseq_raw_data)
-     - [FASTQC](#assay--fastqc)
-
- ## Relationships between Assays and Studies 
+ ### Relationships between Assays and Studies 
  
 ```mermaid
----
-title: Effects_of_Ppd-H1_and_High_Ambient_Temperatures_in_Barley
----
-
 flowchart TD
     classDef investigationStyle fill:#6c7885,color:#2d3e50,fontWeight:bold;
     classDef studyStyle fill:#62d4c1,color:#2d3e50,fontWeight:bold;
     classDef assayStyle fill:#ffd34d,color:#2d3e50,fontWeight:bold;
     classDef processStyle fill:#D46275,color:#2d3e50;
-    id_0["PPD-H1Improves Stress Resistance and Energy Metabolism to Boost Spike Fertility under High Ambient Temperatures"]
+    id_0["PHOTOPERIOD 1 enhances stress tolerance and energy metabolism to promote spike fertility in barley under high ambient temperatures"]
     class id_0 investigationStyle;
     id_0-->STUDY_id_1
     class STUDY_id_1 studyStyle;
@@ -47,114 +30,33 @@ flowchart TD
         id_2[plant-characteristics]
         class id_2 processStyle;
     end
-    subgraph ASSAY_id_3[Assay: Salmon_quantification]
-        id_4[salmon]
+    subgraph ASSAY_id_3[Assay: FASTQC]
+        id_4[FastQC]
         class id_4 processStyle;
     end
     class ASSAY_id_3 assayStyle;
-    subgraph ASSAY_id_5[Assay: RNAseq_analysis_of_MSA]
+    subgraph ASSAY_id_5[Assay: RNAseq_raw_data]
+        id_6[illumina]
+        class id_6 processStyle;
     end
     class ASSAY_id_5 assayStyle;
-    subgraph ASSAY_id_6[Assay: RNAseq_raw_data]
-        id_7[illumina]
-        class id_7 processStyle;
-    end
-    class ASSAY_id_6 assayStyle;
-    subgraph ASSAY_id_8[Assay: RNAseq_analysis_of_leaf]
+    subgraph ASSAY_id_7[Assay: Salmon_quantification]
+        id_8[salmon]
+        class id_8 processStyle;
     end
-    class ASSAY_id_8 assayStyle;
-    subgraph ASSAY_id_9[Assay: FASTQC]
-        id_4[salmon]
-        class id_4 processStyle;
+    class ASSAY_id_7 assayStyle;
+    subgraph ASSAY_id_9[Assay: ThreeDRNAseq]
+        id_10[TPM_leaf]
+        class id_10 processStyle;
+        id_11[CPM_leaf]
+        class id_11 processStyle;
     end
     class ASSAY_id_9 assayStyle;
-    id_2-->|188|id_7
-    id_7-->|382|id_4
-```
-
- 
-### Additional details
-| Meta Data | Description |
-| --------- | ----------- |
-| Submission Date  | tba |
-| Public Release Date | tba |
-| Study identifiers | plant-samples |
-| Study Count | 1 |
-| Assay identifiers | Salmon_quantification , RNAseq_analysis_of_MSA , RNAseq_raw_data , RNAseq_analysis_of_leaf , FASTQC |
-| Assay Count | 5 |
+    id_2-->|188|id_6
+    id_4-->|1|id_4
+    id_6-->|382|id_4
+    id_6-->|382|id_8
+    id_8-->|84|id_10
+    id_8-->|84|id_11
 
-## Contacts 
- 
-| Names | Email | Address | Affiliation | ORCID |
-| ----- | ----- | ------- | ----------- | ----- |
-| Tianyu  Lan | tianyu.lan@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany |  | 
- | Agatha  Walla | Walla@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 40225 Düsseldorf, Germany |  | 
- | Kumsal Ecem  Çolpan Karışan | kumsal.colpan@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 40225 Düsseldorf, Germany |  | 
- | Gabriele  Buchmann | gabriele.buchmann@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany |  | 
- | Vera  Wewer | vwewer@uni-koeln.de |  | CEPLAS Plant Metabolism and Metabolomics Facility, Institute for Plant Sciences, University of Cologne, 50674 Cologne, Germany; Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 40225 Düsseldorf, Germany |  | 
- | Sabine  Metzger | s.metzgar@uni-koeln.de |  | CEPLAS Plant Metabolism and Metabolomics Facility, Institute for Plant Sciences, University of Cologne, 50674 Cologne, Germany; Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 40225 Düsseldorf, Germany |  | 
- | Isaia  Vardanega | isaia.vardanega@hhu.de |  | Institute of Developmental Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany |  | 
- | Einar Baldvin  Haraldsson | einar.haraldsson@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany |  | 
- | Gesa  Helmsorig | gesa.helmsorig@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany |  | 
- | Venkatasubbu  Thirulogachandar | thirulogachandar.venkatasubbu@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany |  | 
- | Rüdiger  Simon | ruediger.simon@hhu.de |  | Institute of Developmental Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 40225 Düsseldorf, Germany |  | 
- | Maria  von Korff | maria.korff.schmissing@hhu.de |  | Institute of Plant Genetics, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 40225 Düsseldorf, Germany |  |
-
-
-## Study: _plant-samples_
-### Description
- tba 
-### Additional details
-| Meta Data | Description |
-| --------- | ----------- |
-| Table Count | 1 |
-| Table Names | plant-characteristics |
-| Sample Count | 188 |
-| Data File Count | 0 |
-| Associated assays | RNAseq_raw_data |
-| Biological replicates | 0 |
-### Annotation headers
-
-**Characteristics**: [organism](https://bioregistry.io/OBI:0100026),[genotype](https://bioregistry.io/EFO:0000513),[biological replicate](https://bioregistry.io/EFO:0002091),[plant structure](https://www.ebi.ac.uk/ols4/ontologies/po/classes/http%253A%252F%252Fpurl.obolibrary.org%252Fobo%252FPO_0009011)
-
-**Factors**: `temperature treatment`,`waddington stage`
-
-## Assay: _Salmon_quantification_
-### Additional details
-| Meta Data | Description |
-| --------- | ----------- |
-| Table Count | 1 |
-| Table Names | NewTable0 |
-| Sample Count | 388 |
-| Data File Count | 388 |
-| Associated studies |  |
-### Annotation headers
-
-## Assay: _RNAseq_raw_data_
-### Additional details
-| Meta Data | Description |
-| --------- | ----------- |
-| Measurement Type | RNA-Seq |
-| Technology Type | Illumina NovaSeq 6000 |
-| Table Count | 1 |
-| Table Names | illumina |
-| Sample Count | 388 |
-| Data File Count | 388 |
-| Associated studies | plant-samples |
-### Annotation headers
-
-**Parameters**: [library strategy](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000035),[library preparation kit](http://purl.obolibrary.org/obo/GENEPIO_0000085),[library preparation kit version](http://purl.obolibrary.org/obo/GENEPIO_0000149),[adapter sequence](http://purl.obolibrary.org/obo/GENEPIO_0000083),[next generation sequencing instrument model](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000040),[base-calling software](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000017),[base-calling software version](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000018),[base-calling software parameters](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000019),[Raw data file format](http://purl.org/nfdi4plants/ontology/dpbo/DPBO_0000021)
-
-## Assay: _FASTQC_
-### Additional details
-| Meta Data | Description |
-| --------- | ----------- |
-| Technology Type | FASTQC |
-| Table Count | 1 |
-| Table Names | NewTable0 |
-| Sample Count | 840 |
-| Data File Count | 0 |
-| Associated studies |  |
-### Annotation headers
-
-**Characteristics**: [Format](https://bioregistry.io/NCIT:C42761)
+```