diff --git a/.gitattributes b/.gitattributes
index a10f4611b81c086c9b32e9f5aae9df0008fa1a42..78c36bc46c7c8962ce1171834d82eb66f59898b3 100644
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@@ -14,10 +14,6 @@ assays/RNAseq/dataset/m_12/m_12_1.fq.gz filter=lfs diff=lfs merge=lfs -text
 assays/RNAseq/dataset/m_17/m_17_2.fq.gz filter=lfs diff=lfs merge=lfs -text
 assays/RNAseq/dataset/m_18/m_18_2.fq.gz filter=lfs diff=lfs merge=lfs -text
 assays/RNAseq/dataset/m_19/m_19_1.fq.gz filter=lfs diff=lfs merge=lfs -text
-assays/RNAseq_analysis_of_leaf/dataset/Count_leaf/counts_gene_leaf.csv filter=lfs diff=lfs merge=lfs -text
-assays/RNAseq_analysis_of_leaf/dataset/Count_leaf/counts_transcript_leaf.csv filter=lfs diff=lfs merge=lfs -text
-assays/RNAseq_analysis_of_leaf/dataset/TPM_leaf/TPM_genes_leaf.csv filter=lfs diff=lfs merge=lfs -text
-assays/RNAseq_analysis_of_leaf/dataset/TPM_leaf/TPM_transcripts_leaf.csv filter=lfs diff=lfs merge=lfs -text
 **/dataset/** filter=lfs diff=lfs merge=lfs -text
 /assays/RNAseq_raw_data/dataset/AT87_EKRN230064181-1A_HWTTFDSX7_L3_1.fq.gz filter=lfs diff=lfs merge=lfs -text
 /assays/RNAseq_raw_data/dataset/AT81_EKRN230064175-1A_HFNGFDSX7_L3_1.fq.gz filter=lfs diff=lfs merge=lfs -text
@@ -413,3 +409,4 @@ assays/FASTQC/dataset/fastqc_raw_RNAseq_data/AT113_EKRN230064191-1A_HFNGFDSX7_L3
 assays/FASTQC/dataset/fastqc_raw_RNAseq_data/AT113_EKRN230064191-1A_HFNGFDSX7_L3_2_fastqc.zip filter=lfs diff=lfs merge=lfs -text
 assays/FASTQC/dataset/fastqc_raw_RNAseq_data/AT113_EKRN230064191-1A_HWTTFDSX7_L3_1_fastqc.html filter=lfs diff=lfs merge=lfs -text
 assays/FASTQC/dataset/fastqc_raw_RNAseq_data/AT113_EKRN230064191-1A_HWTTFDSX7_L3_1_fastqc.zip filter=lfs diff=lfs merge=lfs -text
+assays/ThreeDRNAseq/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
diff --git a/assays/RNAseq_analysis_of_leaf/isa.assay.xlsx b/assays/RNAseq_analysis_of_leaf/isa.assay.xlsx
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diff --git a/assays/RNAseq_analysis_of_leaf/README.md b/assays/ThreeDRNAseq/README.md
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rename from assays/RNAseq_analysis_of_leaf/README.md
rename to assays/ThreeDRNAseq/README.md
index cbf762352c6bbe819864f42a1a0ec2cb2111535e..4aa060d925a359e3a43f0d5c70eee252684cf17a 100644
--- a/assays/RNAseq_analysis_of_leaf/README.md
+++ b/assays/ThreeDRNAseq/README.md
@@ -1,6 +1,6 @@
-RNAseq analysis was conducted on the leaf and main shoot apex (MSA) samples separately.
-
-The tables of Count and Transcript per Million (TPM) were obtained using ThreeDRNAseq R package.
-The tables of differentially expressed genes were obtained using EdgeR R package.
-Gene Ontology analysis was performed using the Triticeae-Gene Tribe database (http://wheat.cau.edu.cn/TGT/m15/?navbar=GOEnrichment).
-WCGNA analysis was performed using the WGCNA R package.
+RNAseq analysis was conducted on the leaf and main shoot apex (MSA) samples separately.
+
+The tables of Count and Transcript per Million (TPM) were obtained using ThreeDRNAseq R package.
+The tables of differentially expressed genes were obtained using EdgeR R package.
+Gene Ontology analysis was performed using the Triticeae-Gene Tribe database (http://wheat.cau.edu.cn/TGT/m15/?navbar=GOEnrichment).
+WCGNA analysis was performed using the WGCNA R package.
diff --git a/assays/RNAseq_analysis_of_leaf/dataset/.gitkeep b/assays/ThreeDRNAseq/dataset/.gitkeep
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diff --git a/assays/RNAseq_analysis_of_leaf/dataset/Count_leaf/counts_gene_leaf.csv b/assays/ThreeDRNAseq/dataset/Count_leaf/counts_gene_leaf.csv
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