Please add a valid title for your ArcInvestigation

---
title: Effects_of_Ppd-H1_and_High_Ambient_Temperatures_in_Barley
---
flowchart TD
    classDef investigationStyle fill:#6c7885,color:#2d3e50,fontWeight:bold;
    classDef studyStyle fill:#62d4c1,color:#2d3e50,fontWeight:bold;
    classDef assayStyle fill:#ffd34d,color:#2d3e50,fontWeight:bold;
    classDef processStyle fill:#D46275,color:#2d3e50;
    id_0["<no-title>"]
    class id_0 investigationStyle;
    id_0-->STUDY_id_1
    class STUDY_id_1 studyStyle;
    subgraph STUDY_id_1[Study: RNAseq_analysis]
    end
    subgraph ASSAY_id_2[Assay: Salmon_quantification]
    end
    class ASSAY_id_2 assayStyle;
    subgraph ASSAY_id_3[Assay: RNAseq_analysis_of_MSA]
    end
    class ASSAY_id_3 assayStyle;
    subgraph ASSAY_id_4[Assay: RNAseq_raw_data]
        id_5[NewTable0]
        class id_5 processStyle;
    end
    class ASSAY_id_4 assayStyle;
    subgraph ASSAY_id_6[Assay: RNAseq_analysis_of_leaf]
    end
    class ASSAY_id_6 assayStyle;
    subgraph ASSAY_id_7[Assay: FASTQC]
        id_5[NewTable0]
        class id_5 processStyle;
    end
    class ASSAY_id_7 assayStyle;
    id_5-->|1|id_5
    id_5-->|389|id_8
    id_8-->|1|id_5
    id_8-->|1|id_8