diff --git a/assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx b/assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx
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index 0000000000000000000000000000000000000000..026c162e4a3a12735226c68f5926fb09cee9ef95
Binary files /dev/null and b/assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx differ
diff --git a/assays/qRT-PCR/protocols/qRT-PCR_primer.txt b/assays/qRT-PCR/protocols/qRT-PCR_primer.txt
new file mode 100644
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+++ b/assays/qRT-PCR/protocols/qRT-PCR_primer.txt
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+Target gene							Primer			Sequence 5’-3’			Fragment size (bp)
+ACT (HORVU.MOREX.r3.5HG0457850)		ACT_591F		CGTGTTGGATTCTGGTGATG	208
+									ACT_789R		AGCCACATATGCGAGCTTCT	
+CO1 (HORVU.MOREX.r3.7HG0671540)		CO1_2185F		CTGCTGGGGCTAGTGCTTAC	251
+									CO1_3454R		CCTTGTTGCATAACGTGTGG	
+CO2 (HORVU.MOREX.r3.6HG0611630)		CO2_564F		AGTGGACTCTTGGCTCCTCA	158
+									CO2_721R		CATGCTGCTGTTCTTGCATT	
+ELF3 (HORVU.MOREX.r3.1HG0095050)	ELF3_DL_3060_F3	TGCTGTCCAAGTGTTTGAGC	242
+									Elf3_DL_4483_R3	CCTGGTTTCCTTCGGTGTTA	
+FT1 (HORVU.MOREX.r3.7HG0653910)		FT1_1955F		GGTAGACCCAGATGCTCCAA	120
+									FT1_2183R		TCGTAGCACATCACCTCCTG	
+GI (HORVU.MOREX.r3.3HG0238250)		GI_6780F		TCAGTTAGAGCTCCTGGAAGT	263
+									GI_7289R		GGTAGTTTGGGCTTTGGATG	
+LHY (HORVU.MOREX.r3.7HG0699010)		HvLHY_672F		CCTGGAATTGGAGATGGAGA	210
+									HvLHY_882R		TGAGCATGGCTTCTGATTTG	
+LUX1 (HORVU.MOREX.r3.3HG0328340)	LUX_1077F		AATTCAGTCCACGGATGCTC	222
+									LUX_1298R		CTTCACTTCAGCTCCCCTTG	
+LWD1 (HORVU.MOREX.r3.6HG0583670)	LWD1_qPCR-114_F	CAGCTCATAGCCCATGACAA	114
+									LWD1_qPCR-114_R	CTCCTTGTCCCGAAGATCAA	
+PHYB (HORVU.MOREX.r3.4HG0381880)	HvPHYB-1151F	CTTGCGCACCAACTATCAGA	232
+									HvPHYB-1383R	CTCCATGACACACCGTCAAC	
+PHYC (HORVU.MOREX.r3.5HG0511090)	PHYC_0986F		ACTACCCGGCAACTGACATC	142
+									PHYC_1127R 		GAGCCACAGAGGCTGATAGG	
+Ppd-H1 (HORVU.MOREX.r3.2HG0107710)	PPD1_2165F		GATGGATTCAAAGGCAAGGA	172
+									PPD1_2336R		GAACAATTGGCTCCTCCAAA	
+PRR1 (HORVU.MOREX.r3.6HG0595250)	HvPRR1_1056F	GAGCATAGCATGGCACTTCA	237
+									HvPRR1_1292R	TGTCTTTCCTCGGAAATTGG	
+PRR59 (HORVU.MOREX.r3.4HG0350690)	PRR59_2064_F	GAAATTCCGCATGAAAAGGA	148
+									PRR59_2212_R	TTCCGCATCTTCTGTTGTTG	
+PRR73 (HORVU.MOREX.r3.5HG0498830)	PRR73_441F		GCGCCGTAGAGAATCAGAAC	222
+									PRR73_662R		CATGTCGGGTACAGTCATCG
+PRR95 (HORVU.MOREX.r3.5HG0498830)	PRR95_1467F		CAGAACTCCAGTGTCGCAAA	251
+									PRR95_1717R		TGCTGTTGCCAGAGTTGTTC	
+VRN1 (HORVU.MOREX.r3.5HG0511210)	HVBM5a_292F		CTGAAGGCGAAGGTTGAGAC	203
+									HVBM5a_494R		TTCTCCTCCTGCAGTGACCT	
\ No newline at end of file