diff --git a/studies/gene_expression_eam7_plants_diurnal/README.md b/studies/gene_expression_eam7_plants_diurnal/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..7d0f2f01ad39ea519680f5eeecd895d65035ba18 100644
--- a/studies/gene_expression_eam7_plants_diurnal/README.md
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+**Test diurnal gene expression of circadian clock and flowering time genes in Bowman and Bowman-derived introgression lines.**
+
+2-week old plants from the genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22.
+
+Gene expression of CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95 and VRN1 were tested.
+
+**The following assays were applied:**
+1. leaf_sampling
+2. RNA_extraction
+3. cDNA_synthesis
+4. qRT-PCR
+
+Results can be found in Figure 3 and in Supplemental Figure S3E.
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