diff --git a/studies/gene_expression_eam7_plants_diurnal/README.md b/studies/gene_expression_eam7_plants_diurnal/README.md index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..7d0f2f01ad39ea519680f5eeecd895d65035ba18 100644 --- a/studies/gene_expression_eam7_plants_diurnal/README.md +++ b/studies/gene_expression_eam7_plants_diurnal/README.md @@ -0,0 +1,13 @@ +**Test diurnal gene expression of circadian clock and flowering time genes in Bowman and Bowman-derived introgression lines.** + +2-week old plants from the genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22. + +Gene expression of CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95 and VRN1 were tested. + +**The following assays were applied:** +1. leaf_sampling +2. RNA_extraction +3. cDNA_synthesis +4. qRT-PCR + +Results can be found in Figure 3 and in Supplemental Figure S3E. \ No newline at end of file