From 9d55e2c0eebaeaea3a4abec0163a5d91a5f88817 Mon Sep 17 00:00:00 2001
From: Gesa Helmsorig <gesa.helmsorig@hhu.de>
Date: Mon, 14 Oct 2024 13:35:09 +0200
Subject: [PATCH] updated readme

---
 .../gene_expression_eam7_plants_diurnal/README.md   | 13 +++++++++++++
 1 file changed, 13 insertions(+)

diff --git a/studies/gene_expression_eam7_plants_diurnal/README.md b/studies/gene_expression_eam7_plants_diurnal/README.md
index e69de29..7d0f2f0 100644
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+**Test diurnal gene expression of circadian clock and flowering time genes in Bowman and Bowman-derived introgression lines.**
+
+2-week old plants from the genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22.
+
+Gene expression of CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95 and VRN1 were tested.
+
+**The following assays were applied:**
+1. leaf_sampling
+2. RNA_extraction
+3. cDNA_synthesis
+4. qRT-PCR
+
+Results can be found in Figure 3 and in Supplemental Figure S3E.
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