diff --git a/assays/cDNA_synthesis/dataset/eam7_diurnal_cDNA_volumes.xlsx b/assays/cDNA_synthesis/dataset/eam7_diurnal_cDNA_volumes.xlsx index 322676467733f75b9bffea7c23ee3452f4ea89b4..88bf5ff454b4d2c948fd306b78f56334290e4de7 100644 Binary files a/assays/cDNA_synthesis/dataset/eam7_diurnal_cDNA_volumes.xlsx and b/assays/cDNA_synthesis/dataset/eam7_diurnal_cDNA_volumes.xlsx differ diff --git a/assays/cDNA_synthesis/dataset/lwd1_diurnal_cDNA_volumes.xlsx b/assays/cDNA_synthesis/dataset/lwd1_diurnal_cDNA_volumes.xlsx index a325c98e48bebe55563ad4029a737f785a6018e0..0071aef4ea33c90e4f92a0a94ea36e797b83bc13 100644 Binary files a/assays/cDNA_synthesis/dataset/lwd1_diurnal_cDNA_volumes.xlsx and b/assays/cDNA_synthesis/dataset/lwd1_diurnal_cDNA_volumes.xlsx differ diff --git a/studies/gene_expression_eam7_plants_diurnal/isa.study.xlsx b/studies/gene_expression_eam7_plants_diurnal/isa.study.xlsx index 8a52a1f35fa2e59743a3a2024c27bb376f543346..7f084a27b0729c1dbb11bde86621fb95fa066dec 100644 Binary files a/studies/gene_expression_eam7_plants_diurnal/isa.study.xlsx and b/studies/gene_expression_eam7_plants_diurnal/isa.study.xlsx differ diff --git a/studies/gene_expression_eam7_plants_diurnal/protocols/development_plant_material_growth_conditions.md b/studies/gene_expression_eam7_plants_diurnal/protocols/development_plant_material_growth_conditions.md new file mode 100644 index 0000000000000000000000000000000000000000..cfb1c70f0280a7a5334500b80e658a0c52a3de4f --- /dev/null +++ b/studies/gene_expression_eam7_plants_diurnal/protocols/development_plant_material_growth_conditions.md @@ -0,0 +1,24 @@ +**eam7 developmental expression: plant material and growth conditions** + +**plant material* + +species: Hordeum vulgare +genotypes: + +- BW: Bowman, spring cultivar +- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) +- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) +- BW(Ppd-H1, eam7): cross of the two introgression lines + + +**growth condtions** + +- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) +- QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3) +- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) + +**sampling** + +- always sample youngest, fully elongated leaf +- sample at ZT 9 (1h after lights turned off, as FT1 expression is highest) +- sample genotypes at 19, 26, 33, 40, 47 and 59 DAE \ No newline at end of file diff --git a/studies/gene_expression_eam7_plants_diurnal/protocols/plant_material_growth_conditions.md b/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md similarity index 81% rename from studies/gene_expression_eam7_plants_diurnal/protocols/plant_material_growth_conditions.md rename to studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md index 88974d69bfb8f2cdf35b05b4c0edee43fee5ed68..38b6565f6642c1ae0e391ec196762fabfb9c52e5 100644 --- a/studies/gene_expression_eam7_plants_diurnal/protocols/plant_material_growth_conditions.md +++ b/studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md @@ -1,4 +1,4 @@ -**Plant material and growth conditions** +**eam7 diurnal expression: plant material and growth conditions** **plant material* @@ -20,7 +20,9 @@ genotypes: **sampling** -sample every 2 hours over 24 hours: +- 2 weeks old plants +- youngest, fully elongated leaf: leaf +- sample every 2 hours over 24 hours: ZT0 (lights are turned on) ZT2 ZT4