diff --git a/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt b/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt index 9b7d39067462c90942447ae3343f88bd67a20948..1d3e55f659fccd96d4215bf7a97d1b91ebd70469 100644 --- a/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt +++ b/assays/biparental_mapping/protocols/CAPS_marker_analysis.txt @@ -20,8 +20,7 @@ M-39 F CTAAATTTCAGCCCCGAACA DdeI 55°C 237,100 334 M-PRR1 F GAGCATAGCATGGCACTTCA Hpy188I 58°C 224, 158 383 R GCCTGCCTAACAAACTGACC lwd1-del F TACCATCCTCCCATTGCCTG - 60°C 199 167 - R ATTGGCTTACACTGCAGGGG - + R ATTGGCTTACACTGCAGGGG Marker Primer Sequence 5’-3’ Enzyme annealing temp (°C) ppd-H1 allele: fragment size (bp) Ppd-H1 allele: fragment size (bp) M-PPDH11 F ACGTGAATGGTGGATCGGC BstUI 60°C 506 433, 73