added plant info

assays/RNA_extraction/protocols/trizol_RNA_extraction.txt

 TRIzol RNA extraction
 
--	Place 2 3mm metal beads into 2.0 ml tube
--	Collect samples directly into the tubes on liquid nitrogen
--	Store samples at -80°C until needed
-
 -	Grind your samples 2 x 45sec at maximum frequency (30 Hertz) in TissueLyser (QIAGEN), use pre-cooled adapters
 -	Add 500 µL of TRIzol to the frozen samples, close tubes and mix vigorously until the sample is homogeneous
 -	Incubate the samples for 5 min at room temperature

studies/gene_expression_eam7_plants/protocols/plant_material_growth_conditions.md

+**Plant material and growth conditions**
+
+**plant material*
+
+species: Hordeum vulgare
+genotypes:
+
+- BW:  Bowman, spring cultivar
+- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(Ppd-H1, eam7): cross of the two introgression lines
+
+
+**growth condtions**
+
+- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+
+**sampling**
+
+sample every 2 hours over 24 hours: 
+	ZT0 (lights are turned on)
+	ZT2
+	ZT4
+	ZT6
+	ZT8 (lights are turned off)
+	ZT10
+	ZT12
+	ZT14
+	ZT16
+	ZT18
+	ZT20
+	ZT22
\ No newline at end of file