From 9ec6c00175b0ef6a91e74aa2718d4a10c7531715 Mon Sep 17 00:00:00 2001
From: Sabrina Zander <sabrina.zander@uni-duesseldorf.de>
Date: Tue, 1 Apr 2025 13:22:59 +0200
Subject: [PATCH] add blots to S5_2 + readme
---
.gitattributes | 6 ++++--
README.md | 2 +-
.../BamHI Southern - 2025-01-20 SYBR Safe stained gel.tif | 3 +++
.../BamHI Southern - 2025-01-22 Chemiluminescence.tif | 3 +++
...lI-SpeI Southern - 2025-01-09 SYBR Safe stained gel.tif} | 0
...=> BclI-SpeI Southern - 2025-01-13 Chemiluminscence.tif} | 0
6 files changed, 11 insertions(+), 3 deletions(-)
create mode 100644 assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-20 SYBR Safe stained gel.tif
create mode 100644 assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-22 Chemiluminescence.tif
rename assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/{2025-01-09 SYBR Safe stained gel.tif => BclI-SpeI Southern - 2025-01-09 SYBR Safe stained gel.tif} (100%)
rename assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/{2025-01-13 Chemiluminscence.tif => BclI-SpeI Southern - 2025-01-13 Chemiluminscence.tif} (100%)
diff --git a/.gitattributes b/.gitattributes
index b65618e..f83f180 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -27,8 +27,6 @@ assays/S4_8_Growth-curves-of-WT-Adea373-and-Adea442/dataset/.gitkeep filter=lfs
assays/S4_8_Growth-curves-of-WT-Adea373-and-Adea442/dataset/Growth[[:space:]]curve_WT-Adea373-Adea442.pzf filter=lfs diff=lfs merge=lfs -text
assays/S5_1_Genomic-map-of-Adea456/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
-assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-09[[:space:]]SYBR[[:space:]]Safe[[:space:]]stained[[:space:]]gel.tif filter=lfs diff=lfs merge=lfs -text
-assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-13[[:space:]]Chemiluminscence.tif filter=lfs diff=lfs merge=lfs -text
assays/S5_3_Growth-curves-of-WT-and-Adea456/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
assays/S5_3_Growth-curves-of-WT-and-Adea456/dataset/Growth[[:space:]]curve_WT-Adea456.pzf filter=lfs diff=lfs merge=lfs -text
assays/S5_4_Epifluorescence-microscopy-of-Adea126-and-Adea456/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
@@ -70,3 +68,7 @@ assays/S4_1_Genomic-map-of-Adea400/dataset/Map_Adea400.jpg filter=lfs diff=lfs m
assays/S4_6_Knock-out-strategy-for-T7RNAP/dataset/Map_Adea442.jpg filter=lfs diff=lfs merge=lfs -text
assays/S5_1_Genomic-map-of-Adea456/dataset/Map_Adea456.jpg filter=lfs diff=lfs merge=lfs -text
assays/S4_6_Knock-out-strategy-for-T7RNAP/dataset/Thumbs.db filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-20[[:space:]]SYBR[[:space:]]Safe[[:space:]]stained[[:space:]]gel.tif filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-22[[:space:]]Chemiluminescence.tif filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-09[[:space:]]SYBR[[:space:]]Safe[[:space:]]stained[[:space:]]gel.tif filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-13[[:space:]]Chemiluminscence.tif filter=lfs diff=lfs merge=lfs -text
diff --git a/README.md b/README.md
index 3aff002..caf060e 100644
--- a/README.md
+++ b/README.md
@@ -58,7 +58,7 @@ The [Assays](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_
### Chapter 5: Analysis of gene expression strength
* [S5_1_Genomic map of Adea456](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_1_Genomic-map-of-Adea456?ref_type=heads) // **Fig. 5A**
-* [S5_2_Verification of insertion by Southern Blot](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_2_Verification-of-insertion-by-Southern-Blot?ref_type=heads) // **Fig. 5B**
+* [S5_2_Verification of insertion by Southern Blot](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_2_Verification-of-insertion-by-Southern-Blot?ref_type=heads) // **Fig. 5B** + supporting material **S1 Figure**
* [S5_3_Growth curves of WT and Adea456](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_3_Growth-curves-of-WT-and-Adea456?ref_type=heads) // **Fig. 5C**
* [S5_4_Epifluorescence microscopy of Adea126 and Adea456](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_4_Epifluorescence-microscopy-of-Adea126-and-Adea456?ref_type=heads) // **Fig. 5D**
* [S5_5_Quantification of fluorescent cells](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_5_Quantification-of-fluorescent-cells?ref_type=heads) // **Fig. 5E**
diff --git a/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-20 SYBR Safe stained gel.tif b/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-20 SYBR Safe stained gel.tif
new file mode 100644
index 0000000..f4bb965
--- /dev/null
+++ b/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-20 SYBR Safe stained gel.tif
@@ -0,0 +1,3 @@
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+size 318138
diff --git a/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-22 Chemiluminescence.tif b/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-22 Chemiluminescence.tif
new file mode 100644
index 0000000..6ea5393
--- /dev/null
+++ b/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI Southern - 2025-01-22 Chemiluminescence.tif
@@ -0,0 +1,3 @@
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diff --git a/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-09 SYBR Safe stained gel.tif b/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI Southern - 2025-01-09 SYBR Safe stained gel.tif
similarity index 100%
rename from assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-09 SYBR Safe stained gel.tif
rename to assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI Southern - 2025-01-09 SYBR Safe stained gel.tif
diff --git a/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-13 Chemiluminscence.tif b/assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI Southern - 2025-01-13 Chemiluminscence.tif
similarity index 100%
rename from assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-13 Chemiluminscence.tif
rename to assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI Southern - 2025-01-13 Chemiluminscence.tif
--
GitLab