From bcbd08b2c01ffbbe5899266223b2253395cc3dc9 Mon Sep 17 00:00:00 2001
From: Dominik Brilhaus <brilhaus@nfdi4plants.org>
Date: Mon, 29 Jan 2024 16:17:07 +0100
Subject: [PATCH] add reads

---
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diff --git a/assays/Brilhaus2015_SE_mRNASeq/protocols/.gitkeep b/assays/Brilhaus2015_SE_mRNASeq/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/Brilhaus2015_SE_mRNASeq/protocols/Illumina.md b/assays/Brilhaus2015_SE_mRNASeq/protocols/Illumina.md
new file mode 100644
index 0000000..fecf1eb
--- /dev/null
+++ b/assays/Brilhaus2015_SE_mRNASeq/protocols/Illumina.md
@@ -0,0 +1,3 @@
+# Preparation and Sequencing of Illumina Libraries
+
+RNA integrity, sequencing library, and fragment size were analyzed on a 2100 Bioanalyzer (Agilent). Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina) and quantified with a Qubit 2.0 (Invitrogen). Samples were multiplexed with 12 libraries per lane and sequenced in single-end mode (Rapid Run, 150 bp read length) on an Illumina HiSeq 2000 platform.
diff --git a/assays/Brilhaus2015_SE_mRNASeq/protocols/RNAex.md b/assays/Brilhaus2015_SE_mRNASeq/protocols/RNAex.md
new file mode 100644
index 0000000..4a87be7
--- /dev/null
+++ b/assays/Brilhaus2015_SE_mRNASeq/protocols/RNAex.md
@@ -0,0 +1,3 @@
+# RNA Extraction
+
+RNA was isolated from ground tissue using the GeneMatrix Universal RNA Purification Kit (EURx Ltd.). Residues of DNA were removed with DNase (New England Biolabs). 
\ No newline at end of file
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