diff --git a/assays/CMML_18-0008_HPLC-DAD/isa.assay.xlsx b/assays/CMML_18-0008_HPLC-DAD/isa.assay.xlsx index 562882d253cf2d1158f8f286b55c7dd7628420fb..a1ffb28027dc7ec8edc23ba3de96b3fa472e4abc 100644 Binary files a/assays/CMML_18-0008_HPLC-DAD/isa.assay.xlsx and b/assays/CMML_18-0008_HPLC-DAD/isa.assay.xlsx differ diff --git a/assays/CMML_18-0008_LCqTOF-MasshunterQuant/isa.assay.xlsx b/assays/CMML_18-0008_LCqTOF-MasshunterQuant/isa.assay.xlsx index e93cd0a823866cc2abea343463f66b7fb9029ca0..570da59b644100c71f7e857d193c5c3887cde825 100644 Binary files a/assays/CMML_18-0008_LCqTOF-MasshunterQuant/isa.assay.xlsx and b/assays/CMML_18-0008_LCqTOF-MasshunterQuant/isa.assay.xlsx differ diff --git a/assays/CMML_18-0008_LCqTOF/isa.assay.xlsx b/assays/CMML_18-0008_LCqTOF/isa.assay.xlsx index 660e9b2d36de9657ea1c7c31d2445eb05d0a92a3..f28d36829a467c3024d618186c301d6ef5c079b8 100644 Binary files a/assays/CMML_18-0008_LCqTOF/isa.assay.xlsx and b/assays/CMML_18-0008_LCqTOF/isa.assay.xlsx differ diff --git a/assays/Maleckova2018_aba_mRNASeq/isa.assay.xlsx b/assays/Maleckova2018_aba_mRNASeq/isa.assay.xlsx index 1f03dc0eb0e84c70177377738e63e95068440a7b..35f1add30e2d7c42f03b31ec010b85c3174bb11a 100644 Binary files a/assays/Maleckova2018_aba_mRNASeq/isa.assay.xlsx and b/assays/Maleckova2018_aba_mRNASeq/isa.assay.xlsx differ diff --git a/assays/TitratableAcidity/README.md b/assays/TitratableAcidity/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/TitratableAcidity/dataset/.gitkeep b/assays/TitratableAcidity/dataset/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/TitratableAcidity/isa.assay.xlsx b/assays/TitratableAcidity/isa.assay.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..328cf699fc0d1dc4817866f586ea16fac00240ff Binary files /dev/null and b/assays/TitratableAcidity/isa.assay.xlsx differ diff --git a/assays/TitratableAcidity/protocols/.gitkeep b/assays/TitratableAcidity/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/assays/TitratableAcidity/protocols/TitratableAcidityProtocol.md b/assays/TitratableAcidity/protocols/TitratableAcidityProtocol.md new file mode 100644 index 0000000000000000000000000000000000000000..15a5cbc8c8427cb11b54a5726a2c2a00a2d4eb9a --- /dev/null +++ b/assays/TitratableAcidity/protocols/TitratableAcidityProtocol.md @@ -0,0 +1,3 @@ +## Titratable acidity + +Approximately 30 mg of ground leaf material was incubated in 500 µl 50% (v/v) methanol at 90 °C while shaking. After centrifugation at 13,000 g for 5 min at room temperature, 450 µl of the supernatant was collected and the remaining pellet was extracted once more. Both fractions were pooled and stored at –20 °C until use. Titratable acidity was measured using bromothymol blue (Carl Roth) as a pH indicator: 60 µl of extract was mixed with 40 µl distilled H2O and 4 µl bromothymol blue (1 mg/ml stock). Using the microinjector of a Synergy H1 (BioTek) plate reader, samples were titrated with 1 mM KOH in 5 µl steps. After each step, absorbance at 445 nm and 615 nm was recorded, and the ratio of the absorbances at 615/445 nm was calculated. Based on the 615/445 ratio of buffer standards (pH 4.6–7.5), the pH of analysed extracts was determined. From the volume of KOH required to titrate extracts to pH 7.0, the titratable acidity was calculated. Two extractions were performed from each sample, and each extract was titrated in three replicates. \ No newline at end of file diff --git a/studies/Maleckova2018_aba/isa.study.xlsx b/studies/Maleckova2018_aba/isa.study.xlsx index 71b351376e487b5e5c895ca49d335025e10e957f..ec9c5f994f98415dc095252cc772152568710352 100644 Binary files a/studies/Maleckova2018_aba/isa.study.xlsx and b/studies/Maleckova2018_aba/isa.study.xlsx differ