diff --git a/.gitattributes b/.gitattributes index a7b52de016f6eaa187fbab4c7db24dc59aa1e12d..5be99d5a806a4393e8ae9fa1e38a43ecce5f13b5 100644 --- a/.gitattributes +++ b/.gitattributes @@ -3,3 +3,4 @@ _publication/Image[[:space:]]1.pdf filter=lfs diff=lfs merge=lfs -text _publication/fpls-09-01709.pdf filter=lfs diff=lfs merge=lfs -text _publication/Data[[:space:]]Sheet[[:space:]]1.xlsx filter=lfs diff=lfs merge=lfs -text studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta filter=lfs diff=lfs merge=lfs -text +runs/kallisto_index/out/kallisto_index filter=lfs diff=lfs merge=lfs -text diff --git a/runs/kallisto_index/out/kallisto_index b/runs/kallisto_index/out/kallisto_index new file mode 100644 index 0000000000000000000000000000000000000000..91b51b9a0c8c7043b347935c5c1ae4dc74d0272e --- /dev/null +++ b/runs/kallisto_index/out/kallisto_index @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:8f1e4d00ef90de6f0b3fc3761e0996eb9e78b3e43eca1f0cb43fee5057f59bb7 +size 973267848 diff --git a/runs/kallisto_index/run.cwl b/runs/kallisto_index/run.cwl index 6cbbb9cb2c9f62d41e333985bac086c08e91b9fa..06490aab04e8ebf21fe039e119d0e48f5a455b85 100644 --- a/runs/kallisto_index/run.cwl +++ b/runs/kallisto_index/run.cwl @@ -28,9 +28,6 @@ outputs: items: Directory outputBinding: glob: $(runtime.outdir)/$(inputs.out_folder) -- id: example_out: - type: stdout -stdout: output.txt baseCommand: - bash diff --git a/runs/kallisto_index/run.log b/runs/kallisto_index/run.log new file mode 100644 index 0000000000000000000000000000000000000000..68630db036b1d2f52df207aa0f89bbd400eaf3e8 --- /dev/null +++ b/runs/kallisto_index/run.log @@ -0,0 +1,63 @@ +[1;30mINFO[0m /Users/dominikbrilhaus/opt/anaconda3/bin/cwltool 3.1.20240112164112 +[1;30mINFO[0m Resolved 'run.cwl' to 'file:///Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/run.cwl' +[1;30mINFO[0m [job run.cwl] /private/tmp/docker_tmp7lobn46w$ docker \ + run \ + -i \ + --mount=type=bind,source=/private/tmp/docker_tmp7lobn46w,target=/OoJBhI \ + --mount=type=bind,source=/private/tmp/docker_tmp18wvdt5m,target=/tmp \ + --mount=type=bind,source=/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/workflows/kallisto_index/kallisto_index.sh,target=/var/lib/cwl/stg35dff622-c131-4e27-9e17-aff52d5d1d4a/kallisto_index.sh,readonly \ + --mount=type=bind,source=/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta,target=/var/lib/cwl/stg1613bcd4-6884-48ed-9ab4-94385e70a5e6/Araport11_genes.201606.cdna.fasta,readonly \ + --workdir=/OoJBhI \ + --read-only=true \ + --net=none \ + --user=502:20 \ + --rm \ + --cidfile=/private/tmp/docker_tmp1e1fablo/20240125124956-101533.cid \ + --env=TMPDIR=/tmp \ + --env=HOME=/OoJBhI \ + quay.io/biocontainers/kallisto:0.43.0--hdf51.8.17_2 \ + bash \ + /var/lib/cwl/stg35dff622-c131-4e27-9e17-aff52d5d1d4a/kallisto_index.sh \ + /var/lib/cwl/stg1613bcd4-6884-48ed-9ab4-94385e70a5e6/Araport11_genes.201606.cdna.fasta \ + ./out +kallisto, version 0.43.0 +When using this program in your research, please cite + + Bray, N. L., Pimentel, H., Melsted, P. & Pachter, L. + Near-optimal probabilistic RNA-seq quantification, + Nature Biotechnology 34, 525-527(2016), doi:10.1038/nbt.3519 + + +[build] loading fasta file /var/lib/cwl/stg1613bcd4-6884-48ed-9ab4-94385e70a5e6/Araport11_genes.201606.cdna.fasta +[build] k-mer length: 31 +[build] warning: clipped off poly-A tail (longer than 10) + from 14 target sequences +[build] warning: replaced 11 non-ACGUT characters in the input sequence + with pseudorandom nucleotides +[build] counting k-mers ... done. +[build] building target de Bruijn graph ... done +[build] creating equivalence classes ... done +[build] target de Bruijn graph has 173467 contigs and contains 45546882 k-mers + +[1;30mINFO[0m [job run.cwl] Max memory used: 4869MiB +[1;30mINFO[0m [job run.cwl] completed success +{ + "outdir": [ + { + "location": "file:///Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out", + "basename": "out", + "class": "Directory", + "listing": [ + { + "class": "File", + "location": "file:///Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out/kallisto_index", + "basename": "kallisto_index", + "size": 973267848, + "checksum": "sha1$e7b7f9828f01a754b0373aaaa793089585580172", + "path": "/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out/kallisto_index" + } + ], + "path": "/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out" + } + ] +}[1;30mINFO[0m Final process status is success diff --git a/runs/kallisto_index/run.yml b/runs/kallisto_index/run.yml index 57e15e409652ed9e212b66d13b72a9930647253e..9f38d9993a7e0e0674554b18cc258aa3382dca3e 100644 --- a/runs/kallisto_index/run.yml +++ b/runs/kallisto_index/run.yml @@ -4,5 +4,5 @@ sh_script: path: ../../workflows/kallisto_index/kallisto_index.sh in_genome_ref: class: File - path: ../../studies/ ##TODO + path: ../../studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta out_folder: ./out