diff --git a/.gitattributes b/.gitattributes index 5be99d5a806a4393e8ae9fa1e38a43ecce5f13b5..c4990cb07d506e64c93272aee82c253303a1b9e1 100644 --- a/.gitattributes +++ b/.gitattributes @@ -4,3 +4,4 @@ _publication/fpls-09-01709.pdf filter=lfs diff=lfs merge=lfs -text _publication/Data[[:space:]]Sheet[[:space:]]1.xlsx filter=lfs diff=lfs merge=lfs -text studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta filter=lfs diff=lfs merge=lfs -text runs/kallisto_index/out/kallisto_index filter=lfs diff=lfs merge=lfs -text +studies/arabidopsis-reference/resources/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa filter=lfs diff=lfs merge=lfs -text diff --git a/studies/arabidopsis-reference/protocols/download-reference.md b/studies/arabidopsis-reference/protocols/download-reference.md new file mode 100644 index 0000000000000000000000000000000000000000..d31d55ff9a8723407dcf0cf9021432cb4d69765e --- /dev/null +++ b/studies/arabidopsis-reference/protocols/download-reference.md @@ -0,0 +1,23 @@ + +# Downloading Arabidopsis TAIR10 fasta + +### Download and unzip + +```bash +curl -O ftp://ftp.ensemblgenomes.org/pub/plants/release-28/fasta/arabidopsis_thaliana/cdna/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa.gz +gunzip Arabidopsis_thaliana.TAIR10.28.cdna.all.fa.gz +``` + +### sanity check + +count number of genes / gene products + +```bash +grep '>' Arabidopsis_thaliana.TAIR10.28.cdna.all.fa | wc -l +``` + +### move + +```bash +mv Arabidopsis_thaliana.TAIR10.28.cdna.all.fa ../resources +``` diff --git a/studies/arabidopsis-reference/resources/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa b/studies/arabidopsis-reference/resources/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa new file mode 100644 index 0000000000000000000000000000000000000000..dce30a46aa82222cbb5bef50b00585585df10441 --- /dev/null +++ b/studies/arabidopsis-reference/resources/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:2924f53c82a7013eeaeb14a6691ba97a65010c540007a3fbeffa40a66ed296e8 +size 73699735 diff --git a/studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta b/studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta deleted file mode 100644 index 51b14af459032b7e302bfee50984f82f2b4406f5..0000000000000000000000000000000000000000 --- a/studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta +++ /dev/null @@ -1,3 +0,0 @@ -version https://git-lfs.github.com/spec/v1 -oid sha256:826d4d9f605007e613e8ba2d2fd186d955a768c5a5cc575998e3f09355137a3d -size 93036871