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# Identification of nuclear pore proteins at plasmodesmata
[![DOI](https://archive.nfdi4plants.org/badge/DOI/10.60534/46qra-6bw98.svg)](https://doi.org/10.60534/46qra-6bw98)
## Abstract
Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
![](_publication/biorxiv-preprint/Figure1.png)
<figcaption>Figure 1. Identification of NUPs at plasmodesmata.</figcaption>
## Preprint
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......@@ -10,4 +10,8 @@ See [2024.09.02.610746v2.full.pdf](biorxiv-preprint/2024.09.02.610746v2.full.pdf
**Identification of nuclear pore proteins at plasmodesmata**
T. Moritz Schladt, Manuel Miras, Jona Obinna Ejike, Mathieu Pottier, Lin Xi, Andrea Restrepo-Escobar, Masayoshi Nakamura, Niklas Pütz, Sebastian Hänsch, Chen Gao, Julia Engelhorn, Marcel Dickmanns, Gwendolyn V. Davis, Ahan Dalal, Sven Gombos, Ronja Lange, Rüdiger Simon, Waltraud X. Schulze, Wolf B. Frommer
bioRxiv 2024.09.02.610746; doi: https://doi.org/10.1101/2024.09.02.610746
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bioRxiv 2024.09.02.610746; doi: https://doi.org/10.1101/2024.09.02.610746
### Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a [CC-BY-NC-ND 4.0 International license](http://creativecommons.org/licenses/by-nc-nd/4.0/).
_publication/biorxiv-preprint/Figure1.png

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