diff --git a/.gitignore b/.gitignore
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diff --git a/assays/dataset/fldpnn/all-disorder-predictions.txt b/assays/dataset/fldpnn/all-disorder-predictions.txt
new file mode 100644
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diff --git a/runs/structure-proportion-extraction.ipynb b/runs/structure-proportion-extraction.ipynb
index ec4fb49d1da30c494da28a588e4fd282c436f5db..5c61255d0060f8fcd85d2e404e0f5767f035457e 100644
--- a/runs/structure-proportion-extraction.ipynb
+++ b/runs/structure-proportion-extraction.ipynb
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-       "Loading extensions from `BioFSharp.Interactive.dll`"
+       "Loading extensions from `Deedle.Interactive.dll`"
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-       "Loading extensions from `Deedle.Interactive.dll`"
+       "Loading extensions from `BioFSharp.Interactive.dll`"
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        "            var config = {\"responsive\":true};\r\n",
-       "            Plotly.newPlot('b6bc9ad4-9c66-4fec-8309-07604ac1d7a1', data, layout, config);\r\n",
+       "            Plotly.newPlot('02594ff6-7545-4dd8-be5e-e29315c888c2', data, layout, config);\r\n",
        "});\r\n",
        "            };\r\n",
        "            if ((typeof(requirejs) !==  typeof(Function)) || (typeof(requirejs.config) !== typeof(Function))) {\r\n",
        "                var script = document.createElement(\"script\");\r\n",
        "                script.setAttribute(\"src\", \"https://cdnjs.cloudflare.com/ajax/libs/require.js/2.3.6/require.min.js\");\r\n",
        "                script.onload = function(){\r\n",
-       "                    renderPlotly_b6bc9ad49c664fec830907604ac1d7a1();\r\n",
+       "                    renderPlotly_02594ff675454dd8be5ee29315c888c2();\r\n",
        "                };\r\n",
        "                document.getElementsByTagName(\"head\")[0].appendChild(script);\r\n",
        "            }\r\n",
        "            else {\r\n",
-       "                renderPlotly_b6bc9ad49c664fec830907604ac1d7a1();\r\n",
+       "                renderPlotly_02594ff675454dd8be5ee29315c888c2();\r\n",
        "            }\r\n",
        "</script>\r\n",
        "\r\n",
@@ -381,6 +381,152 @@
     "//distPlot |> Chart.saveHtml(@\"C:/Users/schne/source/dataplant-gitlab\\yeast-structure-proportions\\assays\\results/structure-proportion-distributions\")\n",
     "//df.SaveCsv(@\"C:/Users/schne/source/dataplant-gitlab\\yeast-structure-proportions\\assays\\results/structure-proportions.csv\")"
    ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "# 4 Performing fldpnn predictions and parsing the results\n",
+    "\n",
+    "fldpnn is a predictor for intrinsically disordered regions (IDR) in proteins. It was performed using the docker container (https://gitlab.com/sina.ghadermarzi/fldpnn_docker/-/tree/master).\n",
+    "\n",
+    "\n",
+    "### 4.1 Splitting the proteome into one fasta file per protein\n",
+    "\n",
+    "fldpnn seems to have problems with large fasta files, therefore a temporary fasta file with only the id as header and sequence as content has been created for each protein. As this would result in > 10k further files to track in the repo, this was done temporarily and ethe results were aggregated in a single result file.\n",
+    "\n",
+    "first, the fasta files were created:"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {
+    "dotnet_interactive": {
+     "language": "fsharp"
+    },
+    "vscode": {
+     "languageId": "dotnet-interactive.fsharp"
+    }
+   },
+   "outputs": [],
+   "source": [
+    "let ensureDirectory p =\n",
+    "    if (not (Directory.Exists(p))) then (Directory.CreateDirectory(p) |> ignore)\n",
+    "\n",
+    "let fsa =\n",
+    "    df\n",
+    "    |> Frame.indexRowsString \"ProteinUId\"\n",
+    "    |> Frame.getCol \"Sequence\"\n",
+    "    |> fun (s:Series<string,string>) ->\n",
+    "        s\n",
+    "        |> Series.observations\n",
+    "        |> Seq.map (fun (h,s) ->\n",
+    "            [FastA.createFastaItem h (s.ToCharArray())]\n",
+    "        )\n",
+    "        |> Array.ofSeq\n",
+    "\n",
+    "// fsa\n",
+    "// |> Array.iter (fun x ->\n",
+    "//     let h = x.[0].Header\n",
+    "//     ensureDirectory $\"\"\"C:/Users/schne/source/dataplant-gitlab\\yeast-structure-proportions\\assays\\dataset/fasta/{h}\"\"\"\n",
+    "//     x\n",
+    "//     |> FastA.write id $\"\"\"C:/Users/schne/source/dataplant-gitlab\\yeast-structure-proportions\\assays\\dataset/fasta/{h}/{h}.fsa\"\"\"\n",
+    "//)"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "subsequently, the fldpnn docker container was used with the ../assays/dataset folder mounted:\n",
+    "\n",
+    "```bash\n",
+    "docker run -it --mount type=bind,source=\"C:\\Users\\schne\\source\\dataplant-gitlab\\yeast-structure-proportions\\assays\\dataset\",target=/home/data sinaghadermarzi/fldpnn\n",
+    "```\n",
+    "\n",
+    " and the flllowing bash script was used to perform predictions for each individual fasta file:\n",
+    "\n",
+    "```bash\n",
+    "for f in ./data/fasta/*/*.fsa; do ./fldpnn/run_flDPnn.py $f; done\n",
+    "```\n",
+    "\n",
+    "the results were subsequently parsed and combined in a single result file:"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {
+    "dotnet_interactive": {
+     "language": "fsharp"
+    },
+    "vscode": {
+     "languageId": "dotnet-interactive.fsharp"
+    }
+   },
+   "outputs": [
+    {
+     "data": {
+      "text/html": [
+       "<table><thead><tr><th>Item1</th><th>Item2</th></tr></thead><tbody><tr><td><div class=\"dni-plaintext\">471</div></td><td><div class=\"dni-plaintext\">5569</div></td></tr></tbody></table>"
+      ]
+     },
+     "metadata": {},
+     "output_type": "display_data"
+    }
+   ],
+   "source": [
+    "let allFldpnnPredictions =\n",
+    "    @\"C:\\Users\\schne\\source\\dataplant-gitlab\\yeast-structure-proportions\\assays\\dataset\\fasta\"\n",
+    "    |> Directory.EnumerateDirectories\n",
+    "    |> Array.ofSeq\n",
+    "\n",
+    "// for some preoteins, fldpnn does not yield disorder predictions.\n",
+    "let noDisorder =\n",
+    "    allFldpnnPredictions\n",
+    "    |> Array.filter (fun x -> Directory.EnumerateFiles(x) |> Seq.length < 4)\n",
+    "    |> Array.map (fun x -> Path.Combine(x,\"results.csv\"))\n",
+    "\n",
+    "let allDisorderPredictions =\n",
+    "    allFldpnnPredictions\n",
+    "    |> Array.filter (fun x -> Directory.EnumerateFiles(x) |> Seq.length > 4)\n",
+    "    |> Array.map (fun x -> Path.Combine(x,\"results.csv\"))\n",
+    "\n",
+    "noDisorder.Length, allDisorderPredictions.Length"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {
+    "dotnet_interactive": {
+     "language": "fsharp"
+    },
+    "vscode": {
+     "languageId": "dotnet-interactive.fsharp"
+    }
+   },
+   "outputs": [],
+   "source": [
+    "// allDisorderPredictions\n",
+    "// |> Array.map (fun d ->\n",
+    "//     File.ReadAllLines(d)\n",
+    "// )\n",
+    "// |> Array.mapi(fun i results ->\n",
+    "//     if i <> 0 then \n",
+    "//         let mutable takeEm = false\n",
+    "//         [|\n",
+    "//             for line in results do\n",
+    "//                 if line.StartsWith(\">\") then takeEm <- true\n",
+    "//                 if takeEm then line\n",
+    "//         |]\n",
+    "//     else \n",
+    "//         results\n",
+    "// )\n",
+    "// |> Array.concat\n",
+    "//|> fun lines -> File.WriteAllLines(\"C:/Users/schne/source/dataplant-gitlab/yeast-structure-proportions/assays/dataset/fldpnn/all-disorder-predictions.txt\",lines)"
+   ]
   }
  ],
  "metadata": {