diff --git a/studies/Bacterial culture conditions/isa.study.xlsx b/studies/Bacterial culture conditions/isa.study.xlsx index 1c3917efc3481af494786ad62f9069fd3a376305..e46bc4b8f6fcef1f64b4a3202631cbe3b3cfb0ac 100644 Binary files a/studies/Bacterial culture conditions/isa.study.xlsx and b/studies/Bacterial culture conditions/isa.study.xlsx differ diff --git a/studies/Plant Growth Conditions/README.md b/studies/Plant Growth Conditions/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/Plant Growth Conditions/isa.study.xlsx b/studies/Plant Growth Conditions/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..56570d4fa38da85863f9e4464ed17bf1fed8ff17 Binary files /dev/null and b/studies/Plant Growth Conditions/isa.study.xlsx differ diff --git a/studies/Plant Growth Conditions/protocols/.gitkeep b/studies/Plant Growth Conditions/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/Plant Growth Conditions/protocols/PlantGrowthConditions.md b/studies/Plant Growth Conditions/protocols/PlantGrowthConditions.md new file mode 100644 index 0000000000000000000000000000000000000000..bea23441d129c454331f47c4867e59898ac8a07e --- /dev/null +++ b/studies/Plant Growth Conditions/protocols/PlantGrowthConditions.md @@ -0,0 +1,4 @@ +## Plant Growth Conditions + + +A. thaliana Col-0 wild-type (N60000) was obtained from the Nottingham Arabidopsis Stock Centre (NASC). *Arabidopsis thaliana* Col-0 seeds were sterilized using 70% ethanol and bleach. Seeds were submerged in 70% ethanol and left shaking at 40 rpm for 14 minutes. Ethanol was removed before the seeds were submerged in 8.3% sodium hypochlorite (Roth) containing 1 μl of Tween 20 (Sigma-Aldrich) and left shaking at 40 rpm for 4 minutes. Under sterile conditions, the seeds were washed 7x times and finally taken up with sterile 10 mM MgC12. Seeds were left for stratification at 4 °C for 3 days. Seed sterility was confirmed by plating approx. 100 seeds on a 50% TSA plate. \ No newline at end of file diff --git a/studies/Plant Growth Conditions/resources/.gitkeep b/studies/Plant Growth Conditions/resources/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391