diff --git a/studies/Bacterial culture conditions/isa.study.xlsx b/studies/Bacterial culture conditions/isa.study.xlsx index e46bc4b8f6fcef1f64b4a3202631cbe3b3cfb0ac..1b5495d777214661aafbddba288560d3f5e7cf65 100644 Binary files a/studies/Bacterial culture conditions/isa.study.xlsx and b/studies/Bacterial culture conditions/isa.study.xlsx differ diff --git a/studies/Plant Growth Conditions/isa.study.xlsx b/studies/Plant Growth Conditions/isa.study.xlsx index 56570d4fa38da85863f9e4464ed17bf1fed8ff17..8097240ae3a7d16bddc619b12feffa897c910052 100644 Binary files a/studies/Plant Growth Conditions/isa.study.xlsx and b/studies/Plant Growth Conditions/isa.study.xlsx differ diff --git a/studies/Plant Growth Conditions/protocols/Agar-media.md b/studies/Plant Growth Conditions/protocols/Agar-media.md new file mode 100644 index 0000000000000000000000000000000000000000..83cfaa61f90146bfd2a60950c48481dcdc9cac02 --- /dev/null +++ b/studies/Plant Growth Conditions/protocols/Agar-media.md @@ -0,0 +1,5 @@ +## Agar-media + +Surface-sterilized and stratified *A. thaliana* Col-0 seeds were sown on plates containing +1% agar (Bacto Agar, Difco) in 1â„2 MS medium supplemented with 0.5% sucrose and placed +vertically in a climate chamber (Panasonic, MLR-352) and grown for six days (10 h light, 21 °C; 14 h dark, 19 °C). Using a forceps, uniform seedlings were then transferred to freshly prepared 1â„2 MS plates without sucrose. \ No newline at end of file diff --git a/studies/Plant Growth Conditions/protocols/Flowpot.md b/studies/Plant Growth Conditions/protocols/Flowpot.md new file mode 100644 index 0000000000000000000000000000000000000000..4fb1763536966015144806718598bb59d4da0bf5 --- /dev/null +++ b/studies/Plant Growth Conditions/protocols/Flowpot.md @@ -0,0 +1,3 @@ +## Flowpot + +Flowpots were assembled and inoculated as described below. Each Flowpot was first flushed with 50 ml sterile MiliQ water and then 50 ml half strength Murashige and Skoog medium with vitamins (1â„2 MS; 2.2 g/l, Duchefa Biochemie, 0.5 g/l MES, pH 5.7) containing the bacterial inoculum. Per Flowpot, five surface-sterilized and stratified *A. thaliana* Col-0 seeds were pipetted. Microboxes were then incubated in a light cabinet under short day conditions (10 h light at 21 °C, 14 h dark at 19 °C) for 14 days and randomized every 2–3 days. \ No newline at end of file