diff --git a/_publication/pnas.2404091121.sd01.txt b/_publication/pnas.2404091121.sd01.txt new file mode 100644 index 0000000000000000000000000000000000000000..89d143fb6ceb4276fa4eba26ee7fca71ce6a64cf --- /dev/null +++ b/_publication/pnas.2404091121.sd01.txt @@ -0,0 +1,10233 @@ +LOCUS pUL123 5772 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert pUL0129 into pUMa3262_pET22b_VBB-NdeI,XhoI% +ACCESSION pUL123 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5772) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: pUMa3262_pET22b_VBB-NdeI,XhoI% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: Insert pUL0129 + Fragment ends: NdeI and XhoI + Fragment size: 413 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..701 + /gene="pET22_n6His_TS_SUMO" + /SECDrawAs="Gene" + CDS 345..749 + /gene="pET22_n6His_TS_SUMO_PAM2 of HsTob " + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..749 + /gene="PAM2 of Hs_Tob" + /SECDrawAs="Gene" + terminator 845..886 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1511..2368 + /gene="bla A - AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4563..5642) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tagcgcgctg agcccgaacg + 721 cgaaagaatt tatttttccg aacatgtaac tcgagcacca ccaccaccac cactgagatc + 781 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac + 841 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa + 901 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt + 961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc + 1021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg + 1081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta + 1141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt + 1201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat + 1261 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa + 1321 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc + 1381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca + 1441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa + 1501 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt + 1561 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca + 1621 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag + 1681 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc + 1741 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca + 1801 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt + 1861 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct + 1921 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt + 1981 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga + 2041 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact + 2101 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc + 2161 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga + 2221 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt + 2281 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga + 2341 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact + 2401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga + 2461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt + 2521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca + 2581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct + 2641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta + 2701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct + 2761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc + 2821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca + 2881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga + 2941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg + 3001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt + 3061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag + 3121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt + 3181 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt + 3241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga + 3301 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca + 3361 ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat + 3421 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg + 3481 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg + 3541 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc + 3601 tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg + 3661 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca + 3721 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt + 3781 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg + 3841 atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc + 3901 gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg + 3961 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg + 4021 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg + 4081 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt + 4141 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca + 4201 cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac + 4261 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg + 4321 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc + 4381 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg + 4441 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg + 4501 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct + 4561 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac + 4621 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag + 4681 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc + 4741 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa + 4801 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc + 4861 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc + 4921 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca + 4981 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc + 5041 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt + 5101 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag + 5161 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta + 5221 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc + 5281 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt + 5341 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc + 5401 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac + 5461 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc + 5521 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg + 5581 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc + 5641 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt + 5701 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag + 5761 gaagcagccc ag +// +LOCUS pUL124 5787 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pul0124 into p3262_VBB% +ACCESSION pUL124 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5787) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_VBB% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pul0124 + Fragment ends: NdeI and XhoI + Fragment size: 422 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..764 + /gene="n6His_TS_SUMO_PAM2L of HsHECT" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 702..764 + /gene="PAM2L of HsHECT" + /SECDrawAs="Gene" + terminator 860..901 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1526..2383 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4578..5657) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgataccgat gatggcgatg + 721 ataacgcgcc gctgttttat cagccgggca aacgcggctt ttaactcgag caccaccacc + 781 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca + 841 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt + 901 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt + 961 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc + 1021 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca + 1081 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc + 1141 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt + 1201 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac + 1261 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc + 1321 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt + 1381 aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt + 1441 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct + 1501 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc + 1561 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa + 1621 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg + 1681 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt + 1741 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg + 1801 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac + 1861 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc + 1921 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa + 1981 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc + 2041 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt + 2101 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga + 2161 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa + 2221 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa + 2281 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa + 2341 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt + 2401 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt + 2461 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg + 2521 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt + 2581 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca + 2641 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac + 2701 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac + 2761 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct + 2821 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg + 2881 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca + 2941 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt + 3001 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta + 3061 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc + 3121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc + 3181 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa + 3241 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag + 3301 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct + 3361 gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca + 3421 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac + 3481 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca + 3541 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga + 3601 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt + 3661 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc + 3721 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt + 3781 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga + 3841 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg + 3901 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta + 3961 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca + 4021 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga + 4081 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct + 4141 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc + 4201 tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct + 4261 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca + 4321 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct + 4381 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga + 4441 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt + 4501 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat + 4561 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt + 4621 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt + 4681 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt + 4741 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt + 4801 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc + 4861 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg + 4921 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga + 4981 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga + 5041 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc + 5101 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta + 5161 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat + 5221 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga + 5281 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag + 5341 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg + 5401 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca + 5461 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa + 5521 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc + 5581 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat + 5641 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc + 5701 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg + 5761 cgactcctgc attaggaagc agcccag +// +LOCUS pUL130 5356 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Gel eluted -Insert for pUL0130 into VBB - p3699% +ACCESSION pUL130 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5356) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB - p3699% + Fragment ends: XhoI and NdeI + Fragment size: 5006 + Insert molecule: Gel eluted -Insert for pUL0130 + Fragment ends: NdeI and XhoI + Fragment size: 350 +FEATURES Location/Qualifiers + CDS 295..1359 + /gene="GST_TS_3xGS_Rrm4_MLLE" + /SECDrawAs="Gene" + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 955..967 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 955..967 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 967..1013 + /gene="GS linker" + /SECDrawAs="Gene" + CDS 1018..1362 + /gene="n6His_Rrm4_MLLE3_679-792AA" + /SECDrawAs="Gene" + CDS 1364..1383 + /gene="GST_TS_3xGGGS" + /SECDrawAs="Gene" + CDS 1801..2661 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3865..4824 + /gene="lacI" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccggtggagg ttctggcggt ggatcgggag gttccatggc acatatgaca + 1021 ttatccacgc ttgctgcgct tcctgcagcc gagattgtgc gcttggccaa ctcccaatcc + 1081 agctccggtc tgcctctccc taaagcggat ccagcgacgg tcaaggcaac cgacgatttc + 1141 atcgactcgc ttcagggcaa agcagcgcac gatcagaagc agaagctggg tgatcagctc + 1201 ttcaaaaaga tccgtacgtt cggcgtcaaa ggcgcaccca agctcaccat ccacttgttg + 1261 gattccgaag acttgcgggc gctggcgcat ctcatgaact cgtacgagga cgtgctcaag + 1321 gaaaaagttc agcacaaggt tgctgcaggt ctgaacaagt gactcgaggc caacgcggcc + 1381 tagtgaattc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa + 1441 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg + 1501 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg + 1561 acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata + 1621 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact + 1681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg + 1741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt + 1801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct + 1861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca + 1921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc + 1981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc + 2041 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg + 2101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta + 2161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc + 2221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt + 2281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg + 2341 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct + 2401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc + 2461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct + 2521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac + 2581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc + 2641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat + 2701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg + 2761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc + 2821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa + 2881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag + 2941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta + 3001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta + 3061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag + 3121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg + 3181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg + 3241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag + 3301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc + 3361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa + 3421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg + 3481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct + 3541 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa + 3601 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat + 3661 tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag + 3721 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc + 3781 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa + 3841 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca + 3901 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg + 3961 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc + 4021 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat + 4081 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc + 4141 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag + 4201 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat + 4261 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg + 4321 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata + 4381 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg + 4441 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca + 4501 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac + 4561 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt + 4621 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg + 4681 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat + 4741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt + 4801 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta + 4861 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg + 4921 ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt + 4981 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc + 5041 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt + 5101 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg + 5161 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc + 5221 agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc + 5281 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg + 5341 aaagctggct acagga +// +LOCUS pUL131 5356 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Gel eluted -Insert for pUL0130 into VBB - p3699% +ACCESSION pUL131 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5356) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB - p3699% + Fragment ends: XhoI and NdeI + Fragment size: 5006 + Insert molecule: Gel eluted -Insert for pUL0130 + Fragment ends: NdeI and XhoI + Fragment size: 350 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + misc_signal 955..967 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 967..1013 + /gene="GS linker" + /SECDrawAs="Gene" + CDS 1018..1362 + /gene="Rrm4_MLLE3_679-792AA" + /SECDrawAs="Gene" + misc_signal 1180..1182 + /gene="Q to A" + /SECDrawAs="Label" + misc_signal 1201..1203 + /gene="F to A" + /SECDrawAs="Label" + CDS 1801..2661 + /gene="AmpR" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccggtggagg ttctggcggt ggatcgggag gttccatggc acatatgaca + 1021 ttatccacgc ttgctgcgct tcctgcagcc gagattgtgc gcttggccaa ctcccaatcc + 1081 agctccggtc tgcctctccc taaagcggat ccagcgacgg tcaaggcaac cgacgatttc + 1141 atcgactcgc ttcagggcaa agcagcgcac gatcagaagg cgaagctggg tgatcagctc + 1201 gccaaaaaga tccgtacgtt cggcgtcaaa ggcgcaccca agctcaccat ccacttgttg + 1261 gattccgaag acttgcgggc gctggcgcat ctcatgaact cgtacgagga cgtgctcaag + 1321 gaaaaagttc agcacaaggt tgctgcaggt ctgaacaagt gactcgaggc caacgcggcc + 1381 tagtgaattc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa + 1441 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg + 1501 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg + 1561 acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata + 1621 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact + 1681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg + 1741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt + 1801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct + 1861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca + 1921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc + 1981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc + 2041 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg + 2101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta + 2161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc + 2221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt + 2281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg + 2341 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct + 2401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc + 2461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct + 2521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac + 2581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc + 2641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat + 2701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg + 2761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc + 2821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa + 2881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag + 2941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta + 3001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta + 3061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag + 3121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg + 3181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg + 3241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag + 3301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc + 3361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa + 3421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg + 3481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct + 3541 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa + 3601 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat + 3661 tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag + 3721 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc + 3781 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa + 3841 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca + 3901 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg + 3961 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc + 4021 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat + 4081 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc + 4141 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag + 4201 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat + 4261 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg + 4321 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata + 4381 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg + 4441 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca + 4501 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac + 4561 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt + 4621 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg + 4681 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat + 4741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt + 4801 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta + 4861 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg + 4921 ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt + 4981 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc + 5041 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt + 5101 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg + 5161 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc + 5221 agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc + 5281 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg + 5341 aaagctggct acagga +// +LOCUS pUL132 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pul0124 into p3262_VBB% +ACCESSION pUL132 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_VBB% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pul0124 + Fragment ends: NdeI and XhoI + Fragment size: 422 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="n6His_TS_SUMO_PAM2-1_UmUpa2" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 702..755 + /gene="PAM2L-Mkrn-Um" + /SECDrawAs="Gene" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg ttgcccgacc tgccgcaccc + 721 cgagcgattt tacctttgtg accccgcagc cgttttaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL133 5778 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pul0124 into p3262_VBB% +ACCESSION pUL133 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5778) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_VBB% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pul0124 + Fragment ends: NdeI and XhoI + Fragment size: 422 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..755 + /gene="n6His_TS_SUMO_PAM2-1_UmUpa2" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 702..752 + /gene="PAM2-Mkrn_Hs" + /SECDrawAs="Gene" + terminator 851..892 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1517..2374 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4569..5648) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgcgggcagc gaagattggg + 721 tgaacgcgat tgaatttgtg ccgggccagc cgtaactcga gcaccaccac caccaccact + 781 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc + 841 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag + 901 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc + 961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc + 1021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa + 1081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact + 1141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt + 1201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa + 1261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt + 1321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac + 1381 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta + 1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata + 1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc + 1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga + 1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct + 1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg + 1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta + 1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat + 1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt + 1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga + 1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga + 2041 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga + 2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc + 2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc + 2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg + 2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat + 2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata + 2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct + 2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga + 2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg + 2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc + 2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct + 2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc + 2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt + 2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg + 2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct + 2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag + 3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag + 3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg + 3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg + 3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac + 3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt + 3301 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat + 3361 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc + 3421 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa + 3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg + 3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga + 3601 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt + 3661 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc + 3721 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat + 3781 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta + 3841 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga + 3901 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg + 3961 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc + 4021 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg + 4081 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg + 4141 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca + 4201 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc + 4261 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga + 4321 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa + 4381 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa + 4441 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc + 4501 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt + 4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg + 4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc + 4681 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag + 4741 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg + 4801 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg + 4861 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc + 4921 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac + 4981 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat + 5041 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc + 5101 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca + 5161 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga + 5221 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg + 5281 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg + 5341 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta + 5401 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc + 5461 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc + 5521 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc + 5581 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact + 5641 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga + 5701 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg + 5761 cattaggaag cagcccag +// +LOCUS pUL134 5778 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pul0124 into p3262_VBB% +ACCESSION pUL134 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5778) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_VBB% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pul0124 + Fragment ends: NdeI and XhoI + Fragment size: 422 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..755 + /gene="n6His_TS_SUMO_PAM2-1_UmUpa2" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 702..752 + /gene="PAM2-Mkrn_Um" + /SECDrawAs="Gene" + terminator 851..892 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1517..2374 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4569..5648) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tagccgcagc aacctgagcg + 721 cgagcgtgca ggcgtttcag ccggtggcgc gctaactcga gcaccaccac caccaccact + 781 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc + 841 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag + 901 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc + 961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc + 1021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa + 1081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact + 1141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt + 1201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa + 1261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt + 1321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac + 1381 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta + 1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata + 1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc + 1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga + 1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct + 1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg + 1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta + 1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat + 1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt + 1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga + 1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga + 2041 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga + 2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc + 2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc + 2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg + 2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat + 2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata + 2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct + 2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga + 2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg + 2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc + 2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct + 2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc + 2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt + 2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg + 2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct + 2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag + 3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag + 3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg + 3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg + 3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac + 3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt + 3301 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat + 3361 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc + 3421 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa + 3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg + 3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga + 3601 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt + 3661 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc + 3721 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat + 3781 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta + 3841 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga + 3901 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg + 3961 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc + 4021 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg + 4081 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg + 4141 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca + 4201 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc + 4261 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga + 4321 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa + 4381 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa + 4441 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc + 4501 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt + 4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg + 4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc + 4681 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag + 4741 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg + 4801 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg + 4861 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc + 4921 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac + 4981 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat + 5041 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc + 5101 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca + 5161 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga + 5221 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg + 5281 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg + 5341 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta + 5401 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc + 5461 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc + 5521 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc + 5581 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact + 5641 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga + 5701 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg + 5761 cattaggaag cagcccag +// +LOCUS pUL138 5754 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pul0124 into p3262_pET22b,NE% +ACCESSION pUL138 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5754) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pul0124 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..731 + /gene="N6xHis_Sumo_PAM2L1_short" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..728 + /gene="PAM2L1_short" + /SECDrawAs="Gene" + terminator 827..868 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1493..2350 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4545..5624) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgacttt gtctacccag + 721 gagccgacta actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc + 781 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg + 841 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga + 901 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt + 961 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct + 1021 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg + 1081 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag + 1141 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa + 1201 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga + 1261 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa + 1321 atttaacgcg aattttaaca aaatattaac gtttacaatt tcaggtggca cttttcgggg + 1381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct + 1441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat + 1501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc + 1561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg + 1621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg + 1681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga + 1741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta + 1801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc + 1861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc + 1921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg + 1981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc + 2041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca + 2101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct + 2161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat + 2221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg + 2281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat + 2341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact + 2401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat + 2461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc + 2521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct + 2581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg + 2641 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca + 2701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc + 2761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga + 2821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac + 2881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga + 2941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag + 3001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg + 3061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag + 3121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc + 3181 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc + 3241 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct + 3301 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact + 3361 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac + 3421 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg + 3481 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt + 3541 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag + 3601 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt + 3661 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt + 3721 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac + 3781 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac + 3841 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca + 3901 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc + 3961 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca + 4021 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt + 4081 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa + 4141 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg + 4201 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag + 4261 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca + 4321 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct + 4381 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc + 4441 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg + 4501 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt + 4561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt + 4621 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg + 4681 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc + 4741 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta + 4801 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg + 4861 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc + 4921 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt + 4981 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga + 5041 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg + 5101 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg + 5161 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca + 5221 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg + 5281 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc + 5341 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc + 5401 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt + 5461 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc + 5521 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag + 5581 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat + 5641 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg + 5701 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccag +// +LOCUS pUL139 5754 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0139 into p3262_pET22b,NE% +ACCESSION pUL139 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5754) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..731 + /gene="N6His_TS_SUMO_PAM2L2-Short" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + misc_feature 702..728 + /gene="PAM2L2" + /SECDrawAs="Region" + CDS 702..728 + /gene="PAM2L2_short" + /SECDrawAs="Gene" + misc_signal 729..731 + /gene="STOP" + /SECDrawAs="Label" + terminator 827..868 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1493..2350 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4545..5624) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgaattc atctacccga + 721 actcgtacta actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc + 781 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg + 841 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga + 901 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt + 961 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct + 1021 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg + 1081 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag + 1141 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa + 1201 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga + 1261 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa + 1321 atttaacgcg aattttaaca aaatattaac gtttacaatt tcaggtggca cttttcgggg + 1381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct + 1441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat + 1501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc + 1561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg + 1621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg + 1681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga + 1741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta + 1801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc + 1861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc + 1921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg + 1981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc + 2041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca + 2101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct + 2161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat + 2221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg + 2281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat + 2341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact + 2401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat + 2461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc + 2521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct + 2581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg + 2641 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca + 2701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc + 2761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga + 2821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac + 2881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga + 2941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag + 3001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg + 3061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag + 3121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc + 3181 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc + 3241 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct + 3301 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact + 3361 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac + 3421 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg + 3481 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt + 3541 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag + 3601 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt + 3661 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt + 3721 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac + 3781 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac + 3841 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca + 3901 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc + 3961 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca + 4021 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt + 4081 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa + 4141 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg + 4201 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag + 4261 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca + 4321 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct + 4381 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc + 4441 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg + 4501 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt + 4561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt + 4621 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg + 4681 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc + 4741 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta + 4801 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg + 4861 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc + 4921 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt + 4981 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga + 5041 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg + 5101 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg + 5161 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca + 5221 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg + 5281 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc + 5341 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc + 5401 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt + 5461 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc + 5521 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag + 5581 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat + 5641 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg + 5701 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccag +// +LOCUS pUL146 5775 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0139 into p3262_pET22b,NE% +ACCESSION pUL146 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5775) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..752 + /gene="N6His_TS_SUMO_PAM2_HsGW182" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..752 + /gene="PAM2_HsGW182" + /SECDrawAs="Gene" + misc_signal 750..752 + /gene="STOP" + /SECDrawAs="Label" + terminator 848..889 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1514..2371 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4566..5645) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg taactggccg ccggaatttc + 721 atccgggcgt gccgtggaaa ggcctgcagt aactcgagca ccaccaccac caccactgag + 781 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat + 841 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag + 901 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg + 961 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt + 1021 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg + 1081 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga + 1141 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac + 1201 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc + 1261 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa + 1321 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat + 1381 ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat + 1441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg + 1501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc + 1561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga + 1621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga + 1681 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg + 1741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc + 1801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac + 1861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact + 1921 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca + 1981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg + 2041 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact + 2101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg + 2161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg + 2221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat + 2281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc + 2341 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat + 2401 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt + 2461 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc + 2521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt + 2581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac + 2641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt + 2701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct + 2761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga + 2821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac + 2881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg + 2941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt + 3001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc + 3061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg + 3121 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc + 3181 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc + 3241 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag + 3301 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc + 3361 acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag + 3421 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac + 3481 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga + 3541 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc + 3601 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat + 3661 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg + 3721 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc + 3781 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg + 3841 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc + 3901 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag + 3961 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg + 4021 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg + 4081 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg + 4141 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga + 4201 gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga + 4261 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata + 4321 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga + 4381 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg + 4441 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca + 4501 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc + 4561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc + 4621 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt + 4681 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg + 4741 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata + 4801 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc + 4861 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc + 4921 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg + 4981 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta + 5041 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg + 5101 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg + 5161 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca + 5221 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc + 5281 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg + 5341 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc + 5401 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc + 5461 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc + 5521 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg + 5581 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt + 5641 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag + 5701 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat + 5761 taggaagcag cccag +// +LOCUS pUL157 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0157 into p3262_pET22b,NE% +ACCESSION pUL157 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="N6His_TS_SUMO_P2-P2L2hybrid_UmUpa1" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="P2-P2L2hybofUmUpa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg ttctcagtct accctgtctc + 721 cgaacgcgga cgaattcatc tacccgaact cttactaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL162 5778 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0162 into p3262_pET22b,NE% +ACCESSION pUL162 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5778) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..755 + /gene="N6His_TS_SUMO_PAM2_HsPaip2" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..752 + /gene="PAM2_HsPaip2" + /SECDrawAs="Gene" + misc_signal 753..755 + /gene="STOP" + /SECDrawAs="Label" + terminator 851..892 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1517..2374 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4569..5648) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgttaaatct aacctgaacc + 721 cgaacgcgaa agaattcgtt ccgggtgtta aataactcga gcaccaccac caccaccact + 781 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc + 841 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag + 901 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc + 961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc + 1021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa + 1081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact + 1141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt + 1201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa + 1261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt + 1321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac + 1381 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta + 1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata + 1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc + 1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga + 1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct + 1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg + 1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta + 1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat + 1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt + 1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga + 1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga + 2041 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga + 2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc + 2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc + 2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg + 2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat + 2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata + 2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct + 2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga + 2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg + 2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc + 2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct + 2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc + 2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt + 2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg + 2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct + 2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag + 3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag + 3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg + 3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg + 3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac + 3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt + 3301 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat + 3361 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc + 3421 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa + 3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg + 3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga + 3601 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt + 3661 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc + 3721 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat + 3781 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta + 3841 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga + 3901 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg + 3961 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc + 4021 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg + 4081 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg + 4141 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca + 4201 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc + 4261 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga + 4321 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa + 4381 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa + 4441 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc + 4501 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt + 4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg + 4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc + 4681 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag + 4741 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg + 4801 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg + 4861 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc + 4921 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac + 4981 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat + 5041 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc + 5101 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca + 5161 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga + 5221 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg + 5281 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg + 5341 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta + 5401 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc + 5461 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc + 5521 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc + 5581 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact + 5641 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga + 5701 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg + 5761 cattaggaag cagcccag +// +LOCUS pUL166 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0166 into p3262_pET22b,NE% +ACCESSION pUL166 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="N6His_TS_SUMO_FP/AA-PAM2L2_UmUpa1" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="Y/A PAM2L1_Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgaagcggcg gatcaggaag + 721 aagatcagga tgattttgtg gcgccgggcg cggattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL167 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0161 into p3262_pET22b,NE% +ACCESSION pUL167 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="N6His_TS_SUMO_FP/AA-PAM2L2_UmUpa1" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="Y/A_PAM2l2_Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgaagat gcggcggatg + 721 atgatgatga tgaatttatt gcgccgaaca gctattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL168 5778 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0161 into p3262_pET22b,NE% +ACCESSION pUL168 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5778) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..755 + /gene="N6His_TS_SUMO_FP/AA-PAM2L2_UmUpa1" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..752 + /gene="PL2-P2hyb3-Upa1" + /SECDrawAs="Gene" + misc_signal 753..755 + /gene="STOP" + /SECDrawAs="Label" + terminator 851..892 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1517..2374 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4569..5648) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgaagat gcggcggatg + 721 atgatgatag cgtgtttaaa ccgagccgca gctaactcga gcaccaccac caccaccact + 781 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc + 841 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag + 901 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc + 961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc + 1021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa + 1081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact + 1141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt + 1201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa + 1261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt + 1321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac + 1381 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta + 1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata + 1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc + 1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga + 1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct + 1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg + 1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta + 1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat + 1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt + 1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga + 1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga + 2041 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga + 2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc + 2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc + 2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg + 2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat + 2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata + 2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct + 2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga + 2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg + 2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc + 2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct + 2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc + 2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt + 2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg + 2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct + 2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag + 3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag + 3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg + 3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg + 3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac + 3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt + 3301 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat + 3361 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc + 3421 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa + 3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg + 3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga + 3601 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt + 3661 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc + 3721 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat + 3781 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta + 3841 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga + 3901 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg + 3961 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc + 4021 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg + 4081 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg + 4141 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca + 4201 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc + 4261 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga + 4321 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa + 4381 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa + 4441 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc + 4501 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt + 4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg + 4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc + 4681 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag + 4741 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg + 4801 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg + 4861 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc + 4921 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac + 4981 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat + 5041 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc + 5101 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca + 5161 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga + 5221 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg + 5281 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg + 5341 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta + 5401 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc + 5461 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc + 5521 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc + 5581 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact + 5641 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga + 5701 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg + 5761 cattaggaag cagcccag +// +LOCUS pUL169 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0169 into p3262_pET22b,NE% +ACCESSION pUL169 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="HS-PAM2L1 (Fto A)_Upa1" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="F/A PAM2L1_Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgaagcggcg gatcaggaag + 721 aagatcagga tgatgcggtg tatccgggcg cggattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL170 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0170 into p3262_pET22b,NE% +ACCESSION pUL170 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="HS-PAM2L2 (FtoA)_Upa1" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="F/APAM2L2-Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgaagat gcggcggatg + 721 atgatgatga tgaagcgatt tatccgaaca gctattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL171 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0171 into p3262_pET22b,NE% +ACCESSION pUL171 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="HS-PAM2L1(PtoA)" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="P/APAM2L1-Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgaagcggcg gatcaggaag + 721 aagatcagga tgattttgtg tatgcgggcg cggattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL172 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0172 into p3262_pET22b,NE% +ACCESSION pUL172 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="P/A PAM2L2-Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgaagat gcggcggatg + 721 atgatgatga tgaatttatt tatgcgaaca gctattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL173 5778 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0173 into p3262_pET22b,NE% +ACCESSION pUL173 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5778) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..752 + /gene="F/A PAM2-Upa1" + /SECDrawAs="Gene" + misc_signal 753..755 + /gene="STOP" + /SECDrawAs="Label" + terminator 851..892 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1517..2374 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4569..5648) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tagccagagc accctgagcc + 721 cgaacgcgag cgtggcgaaa ccgagccgca gctaactcga gcaccaccac caccaccact + 781 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc + 841 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag + 901 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc + 961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc + 1021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa + 1081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact + 1141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt + 1201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa + 1261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt + 1321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac + 1381 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta + 1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata + 1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc + 1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga + 1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct + 1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg + 1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta + 1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat + 1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt + 1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga + 1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga + 2041 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga + 2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc + 2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc + 2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg + 2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat + 2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata + 2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct + 2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga + 2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg + 2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc + 2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct + 2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc + 2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt + 2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg + 2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct + 2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag + 3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag + 3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg + 3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg + 3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac + 3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt + 3301 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat + 3361 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc + 3421 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa + 3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg + 3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga + 3601 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt + 3661 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc + 3721 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat + 3781 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta + 3841 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga + 3901 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg + 3961 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc + 4021 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg + 4081 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg + 4141 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca + 4201 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc + 4261 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga + 4321 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa + 4381 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa + 4441 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc + 4501 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt + 4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg + 4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc + 4681 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag + 4741 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg + 4801 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg + 4861 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc + 4921 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac + 4981 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat + 5041 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc + 5101 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca + 5161 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga + 5221 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg + 5281 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg + 5341 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta + 5401 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc + 5461 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc + 5521 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc + 5581 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact + 5641 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga + 5701 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg + 5761 cattaggaag cagcccag +// +LOCUS pUL174 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0174 into p3262_pET22b,NE% +ACCESSION pUL174 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="HS-PAM2L1 ( D to A )" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="D/A PAM2L1-Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgaagcggcg gatcaggaag + 721 aagatcaggc ggattttgtg tatccgggcg cggattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL175 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0175 into p3262_pET22b,NE% +ACCESSION pUL175 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="HS-PAM2L2 ( D to A)" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="D/A PAM2L2-Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgaagat gcggcggatg + 721 atgatgatgc ggaatttatt tatccgaaca gctattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL198 5356 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Gel eluted -Insert for pUL0130 into VBB - p3699% +ACCESSION pUL198 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5356) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB - p3699% + Fragment ends: XhoI and NdeI + Fragment size: 5006 + Insert molecule: Gel eluted -Insert for pUL0130 + Fragment ends: NdeI and XhoI + Fragment size: 350 +FEATURES Location/Qualifiers + CDS 295..1359 + /gene="GST_TS_3xGS_Rrm4_MLLE" + /SECDrawAs="Gene" + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 955..967 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 955..967 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 967..1013 + /gene="GS linker" + /SECDrawAs="Gene" + CDS 1018..1359 + /gene="MLLE3 ( H to A )" + /SECDrawAs="Gene" + misc_signal 1168..1170 + /gene="H to A" + /product="H to A point mutation" + /SECDrawAs="Label" + CDS 1364..1383 + /gene="GST_TS_3xGGGS" + /SECDrawAs="Gene" + CDS 1801..2661 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3865..4824 + /gene="lacI" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccggtggagg ttctggcggt ggatcgggag gttccatggc acatatgaca + 1021 ttatccacgc ttgctgcgct tcctgcagcc gagattgtgc gcttggccaa ctcccaatcc + 1081 agctccggtc tgcctctccc taaagcggat ccagcgacgg tcaaggcaac cgacgatttc + 1141 atcgactcgc ttcagggcaa agcagcggcg gatcagaagc agaagctggg tgatcagctc + 1201 ttcaaaaaga tccgtacgtt cggcgtcaaa ggcgcaccca agctcaccat ccacttgttg + 1261 gattccgaag acttgcgggc gctggcgcat ctcatgaact cgtacgagga cgtgctcaag + 1321 gaaaaagttc agcacaaggt tgctgcaggt ctgaacaagt gactcgaggc caacgcggcc + 1381 tagtgaattc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa + 1441 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg + 1501 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg + 1561 acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata + 1621 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact + 1681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg + 1741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt + 1801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct + 1861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca + 1921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc + 1981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc + 2041 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg + 2101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta + 2161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc + 2221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt + 2281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg + 2341 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct + 2401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc + 2461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct + 2521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac + 2581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc + 2641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat + 2701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg + 2761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc + 2821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa + 2881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag + 2941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta + 3001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta + 3061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag + 3121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg + 3181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg + 3241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag + 3301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc + 3361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa + 3421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg + 3481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct + 3541 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa + 3601 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat + 3661 tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag + 3721 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc + 3781 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa + 3841 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca + 3901 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg + 3961 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc + 4021 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat + 4081 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc + 4141 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag + 4201 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat + 4261 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg + 4321 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata + 4381 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg + 4441 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca + 4501 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac + 4561 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt + 4621 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg + 4681 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat + 4741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt + 4801 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta + 4861 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg + 4921 ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt + 4981 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc + 5041 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt + 5101 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg + 5161 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc + 5221 agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc + 5281 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg + 5341 aaagctggct acagga +// +LOCUS pUL200 5365 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert into VBB +ACCESSION pUL200 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5365) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB + Fragment ends: XhoI and NcoI + Fragment size: 4964 + Insert molecule: insert + Fragment ends: NcoI and XhoI + Fragment size: 401 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 295..1371 + /gene="GST_Rrm4_MLLE3 (R744 to A)" + /SECDrawAs="Gene" + misc_signal 295..297 + /gene="Start codon" + /SECDrawAs="Label" + misc_feature 295..297 + /gene="Start codon" + /SECDrawAs="Region" + CDS 955..971 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 955..971 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 982..1371 + /gene="Rrm4_MLLE3(R744 to A)" + /SECDrawAs="Gene" + CDS 1810..2670 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3874..4833 + /gene="lacI" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccatgggagc tagcgcggcc gccagctcca tctcgcctgg cgctgctgag + 1021 gcttacacat tatccacgct tgctgcgctt cctgcagccg agattgtgcg cttggccaac + 1081 tcccaatcca gctccggtct gcctctccct aaagcggatc cagcgacggt caaggcaacc + 1141 gacgatttca tcgactcgct tcagggcaaa gcagcgcacg atcagaagca gaagctgggt + 1201 gatcagctct tcaaaaagat cgctacgttc ggcgtcaaag gcgcacccaa gctcaccatc + 1261 cacttgttgg attccgaaga cttgcgggcg ctggcgcatc tcatgaactc gtacgaggac + 1321 gtgctcaagg aaaaagttca gcacaaggtt gctgcaggtc tgaacaagtg actcgaggcc + 1381 aacgcggcct agtgaattca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat + 1441 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg + 1501 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc + 1561 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc + 1621 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca + 1681 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat + 1741 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa + 1801 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt + 1861 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag + 1921 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt + 1981 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg + 2041 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag + 2101 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta + 2161 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg + 2221 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta + 2281 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac + 2341 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt + 2401 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca + 2461 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag + 2521 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta + 2581 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag + 2641 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt + 2701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat + 2761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta + 2821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa + 2881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt + 2941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag + 3001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta + 3061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca + 3121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag + 3181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa + 3241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga + 3301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc + 3361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc + 3421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt + 3481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt + 3541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag + 3601 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac + 3661 cgcataaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc + 3721 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca + 3781 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt + 3841 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac + 3901 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt + 3961 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg + 4021 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg + 4081 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac + 4141 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc + 4201 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc + 4261 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt + 4321 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt + 4381 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg + 4441 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg + 4501 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta + 4561 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa + 4621 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc + 4681 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg + 4741 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca + 4801 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct + 4861 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat + 4921 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg + 4981 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg + 5041 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt + 5101 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag + 5161 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct + 5221 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta + 5281 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta + 5341 atgttgatga aagctggcta cagga +// +LOCUS pUL201 5365 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert into VBB +ACCESSION pUL201 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5365) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB + Fragment ends: XhoI and NcoI + Fragment size: 4964 + Insert molecule: insert + Fragment ends: NcoI and XhoI + Fragment size: 401 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 295..1371 + /gene="GST_Rrm4_MLLE I 756 to G" + /SECDrawAs="Gene" + misc_signal 295..297 + /gene="Start codon" + /SECDrawAs="Label" + misc_feature 295..297 + /gene="Start codon" + /SECDrawAs="Region" + CDS 955..971 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 955..971 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 982..1371 + /gene="Rrm4_MLLE3(I756 to G)" + /SECDrawAs="Gene" + misc_signal 1172..1368 + /gene="MLLE3" + /SECDrawAs="Label" + CDS 1810..2670 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3874..4833 + /gene="lacI" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccatgggagc tagcgcggcc gccagctcca tctcgcctgg cgctgctgag + 1021 gcttacacat tatccacgct tgctgcgctt cctgcagccg agattgtgcg cttggccaac + 1081 tcccaatcca gctccggtct gcctctccct aaagcggatc cagcgacggt caaggcaacc + 1141 gacgatttca tcgactcgct tcagggcaaa gcagcgcacg atcagaagca gaagctgggt + 1201 gatcagctct tcaaaaagat ccgtacgttc ggcgtcaaag gcgcacccaa gctcaccggc + 1261 cacttgttgg attccgaaga cttgcgggcg ctggcgcatc tcatgaactc gtacgaggac + 1321 gtgctcaagg aaaaagttca gcacaaggtt gctgcaggtc tgaacaagtg actcgaggcc + 1381 aacgcggcct agtgaattca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat + 1441 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg + 1501 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc + 1561 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc + 1621 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca + 1681 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat + 1741 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa + 1801 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt + 1861 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag + 1921 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt + 1981 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg + 2041 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag + 2101 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta + 2161 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg + 2221 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta + 2281 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac + 2341 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt + 2401 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca + 2461 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag + 2521 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta + 2581 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag + 2641 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt + 2701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat + 2761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta + 2821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa + 2881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt + 2941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag + 3001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta + 3061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca + 3121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag + 3181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa + 3241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga + 3301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc + 3361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc + 3421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt + 3481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt + 3541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag + 3601 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac + 3661 cgcataaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc + 3721 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca + 3781 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt + 3841 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac + 3901 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt + 3961 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg + 4021 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg + 4081 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac + 4141 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc + 4201 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc + 4261 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt + 4321 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt + 4381 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg + 4441 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg + 4501 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta + 4561 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa + 4621 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc + 4681 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg + 4741 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca + 4801 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct + 4861 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat + 4921 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg + 4981 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg + 5041 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt + 5101 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag + 5161 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct + 5221 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta + 5281 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta + 5341 atgttgatga aagctggcta cagga +// +LOCUS pUL202 5778 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pul0202 into p3262_VBB% +ACCESSION pUL202 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5778) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_VBB% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pul0124 + Fragment ends: NdeI and XhoI + Fragment size: 422 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..755 + /gene="n6His_TS_SUMO_PAM2-1_UmUpa2" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 702..752 + /gene="PAM2_LtoA_Upa1" + /SECDrawAs="Gene" + misc_signal 714..716 + /gene="(null)" + /SECDrawAs="Label" + terminator 851..892 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1517..2374 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4569..5648) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg ttcgcaaagc accgcgtctc + 721 ccaacgcctc cgtcttcaag ccgtcccgat cctaactcga gcaccaccac caccaccact + 781 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc + 841 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag + 901 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc + 961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc + 1021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa + 1081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact + 1141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt + 1201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa + 1261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt + 1321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac + 1381 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta + 1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata + 1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc + 1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga + 1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct + 1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg + 1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta + 1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat + 1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt + 1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga + 1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga + 2041 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga + 2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc + 2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc + 2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg + 2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat + 2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata + 2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct + 2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga + 2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg + 2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc + 2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct + 2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc + 2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt + 2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg + 2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct + 2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag + 3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag + 3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg + 3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg + 3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac + 3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt + 3301 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat + 3361 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc + 3421 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa + 3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg + 3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga + 3601 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt + 3661 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc + 3721 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat + 3781 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta + 3841 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga + 3901 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg + 3961 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc + 4021 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg + 4081 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg + 4141 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca + 4201 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc + 4261 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga + 4321 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa + 4381 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa + 4441 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc + 4501 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt + 4561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg + 4621 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc + 4681 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag + 4741 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg + 4801 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg + 4861 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc + 4921 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac + 4981 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat + 5041 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc + 5101 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca + 5161 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga + 5221 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg + 5281 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg + 5341 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta + 5401 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc + 5461 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc + 5521 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc + 5581 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact + 5641 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga + 5701 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg + 5761 cattaggaag cagcccag +// +LOCUS pUL203 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert pUL0129 into pUMa3262_pET22b_VBB-NdeI,XhoI% +ACCESSION pUL203 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: pUMa3262_pET22b_VBB-NdeI,XhoI% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: Insert pUL203 + Fragment ends: NdeI and XhoI + Fragment size: 413 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..701 + /gene="pET22_n6His_TS_SUMO" + /SECDrawAs="Gene" + misc_feature 345..758 + /gene="pET22_n6His_TS_SUMO_PAM2 FKYP " + /SECDrawAs="Info only" + CDS 345..758 + /gene="N6-his_SUMO_PAM2-FKYP" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="PAM2_FKYp" + /SECDrawAs="Gene" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="bla A - AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg ttcgcaaagc accttgtctc + 721 ccaacgcctc cgtcttcaag tacccgtccc gatcctaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL205 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert pUL0129 into pUMa3262_pET22b_VBB-NdeI,XhoI% +ACCESSION pUL205 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: pUMa3262_pET22b_VBB-NdeI,XhoI% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: Insert pUL0129 + Fragment ends: NdeI and XhoI + Fragment size: 413 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..701 + /gene="pET22_n6His_TS_SUMO" + /SECDrawAs="Gene" + misc_feature 345..758 + /gene="pET22_n6His_TS_SUMO_PAM2 FKYP " + /SECDrawAs="Info only" + CDS 345..758 + /gene="N6-his_SUMO_PAM2L-HsMKrn" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="PAM2L_HsMkrn" + /SECDrawAs="Gene" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="bla A - AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg taaaagctgc ccggaatgcc + 721 gcattaccag caactttgtg attccgagcg aatattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL206 5365 bp DNA circular SYN 14-AUG-2024 +DEFINITION Ligation of insert into VBB +ACCESSION pUL206 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5365) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB + Fragment ends: XhoI and NcoI + Fragment size: 4964 + Insert molecule: insert + Fragment ends: NcoI and XhoI + Fragment size: 401 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 295..1371 + /gene="GST_Rrm4_MLLE3 (Q733 toA)" + /SECDrawAs="Gene" + misc_signal 295..297 + /gene="Start codon" + /SECDrawAs="Label" + misc_feature 295..297 + /gene="Start codon" + /SECDrawAs="Region" + CDS 955..971 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 955..971 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 1027..1371 + /gene="Rrm4_MLLE3(Q733 to A)" + /SECDrawAs="Gene" + misc_signal 1027..1368 + /gene="MLLE3" + /SECDrawAs="Label" + misc_feature 1027..1368 + /gene="MLLE3" + /SECDrawAs="Region" + misc_feature 1189..1191 + /gene="Q733A" + /SECDrawAs="Region" + CDS 1810..2670 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3874..4833 + /gene="lacI" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccatgggagc tagcgcggcc gccagctcca tctcgcctgg cgctgctgag + 1021 gcttacacat tatccacgct tgctgcgctt cctgcagccg agattgtgcg cttggccaac + 1081 tcccaatcca gctccggtct gcctctccct aaagcggatc cagcgacggt caaggcaacc + 1141 gacgatttca tcgactcgct tcagggcaaa gcagcgcacg atcagaaggc gaagctgggt + 1201 gatcagctct tcaaaaagat ccgtacgttc ggcgtcaaag gcgcacccaa gctcaccatc + 1261 cacttgttgg attccgaaga cttgcgggcg ctggcgcatc tcatgaactc gtacgaggac + 1321 gtgctcaagg aaaaagttca gcacaaggtt gctgcaggtc tgaacaagtg actcgaggcc + 1381 aacgcggcct agtgaattca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat + 1441 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg + 1501 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc + 1561 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc + 1621 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca + 1681 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat + 1741 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa + 1801 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt + 1861 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag + 1921 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt + 1981 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg + 2041 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag + 2101 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta + 2161 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg + 2221 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta + 2281 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac + 2341 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt + 2401 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca + 2461 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag + 2521 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta + 2581 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag + 2641 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt + 2701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat + 2761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta + 2821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa + 2881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt + 2941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag + 3001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta + 3061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca + 3121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag + 3181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa + 3241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga + 3301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc + 3361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc + 3421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt + 3481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt + 3541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag + 3601 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac + 3661 cgcataaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc + 3721 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca + 3781 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt + 3841 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac + 3901 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt + 3961 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg + 4021 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg + 4081 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac + 4141 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc + 4201 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc + 4261 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt + 4321 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt + 4381 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg + 4441 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg + 4501 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta + 4561 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa + 4621 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc + 4681 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg + 4741 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca + 4801 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct + 4861 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat + 4921 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg + 4981 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg + 5041 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt + 5101 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag + 5161 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct + 5221 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta + 5281 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta + 5341 atgttgatga aagctggcta cagga +// +LOCUS pUL207 5365 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert into VBB +ACCESSION pUL207 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5365) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB + Fragment ends: XhoI and NcoI + Fragment size: 4964 + Insert molecule: insert + Fragment ends: NcoI and XhoI + Fragment size: 401 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 295..1371 + /gene="GST_Rrm4_MLLE3 (F740 to A)" + /SECDrawAs="Gene" + misc_signal 295..297 + /gene="Start codon" + /SECDrawAs="Label" + misc_feature 295..297 + /gene="Start codon" + /SECDrawAs="Region" + CDS 955..971 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 955..971 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 982..1371 + /gene="Rrm4_MLLE3(F740 to A)" + /SECDrawAs="Gene" + misc_feature 1172..1368 + /gene="MLLE3" + /SECDrawAs="Region" + misc_signal 1172..1368 + /gene="MLLE3" + /SECDrawAs="Label" + misc_signal 1210..1212 + /gene="F740 to A" + /SECDrawAs="Label" + CDS 1810..2670 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3874..4833 + /gene="lacI" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccatgggagc tagcgcggcc gccagctcca tctcgcctgg cgctgctgag + 1021 gcttacacat tatccacgct tgctgcgctt cctgcagccg agattgtgcg cttggccaac + 1081 tcccaatcca gctccggtct gcctctccct aaagcggatc cagcgacggt caaggcaacc + 1141 gacgatttca tcgactcgct tcagggcaaa gcagcgcacg atcagaagca gaagctgggt + 1201 gatcagctcg ccaaaaagat ccgtacgttc ggcgtcaaag gcgcacccaa gctcaccatc + 1261 cacttgttgg attccgaaga cttgcgggcg ctggcgcatc tcatgaactc gtacgaggac + 1321 gtgctcaagg aaaaagttca gcacaaggtt gctgcaggtc tgaacaagtg actcgaggcc + 1381 aacgcggcct agtgaattca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat + 1441 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg + 1501 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc + 1561 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc + 1621 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca + 1681 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat + 1741 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa + 1801 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt + 1861 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag + 1921 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt + 1981 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg + 2041 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag + 2101 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta + 2161 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg + 2221 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta + 2281 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac + 2341 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt + 2401 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca + 2461 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag + 2521 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta + 2581 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag + 2641 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt + 2701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat + 2761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta + 2821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa + 2881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt + 2941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag + 3001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta + 3061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca + 3121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag + 3181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa + 3241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga + 3301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc + 3361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc + 3421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt + 3481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt + 3541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag + 3601 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac + 3661 cgcataaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc + 3721 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca + 3781 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt + 3841 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac + 3901 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt + 3961 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg + 4021 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg + 4081 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac + 4141 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc + 4201 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc + 4261 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt + 4321 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt + 4381 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg + 4441 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg + 4501 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta + 4561 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa + 4621 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc + 4681 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg + 4741 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca + 4801 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct + 4861 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat + 4921 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg + 4981 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg + 5041 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt + 5101 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag + 5161 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct + 5221 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta + 5281 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta + 5341 atgttgatga aagctggcta cagga +// +LOCUS pUL211 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert pUL0170 into p3262_pET22b,NE% +ACCESSION pUL211 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3262_pET22b,NE% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: insert pUL0139 + Fragment ends: NdeI and XhoI + Fragment size: 389 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..758 + /gene="N6his-SUMO-PAM2L2 (A to L)" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="A/L PAM2L2-Upa1" + /SECDrawAs="Gene" + misc_signal 756..758 + /gene="STOP" + /SECDrawAs="Label" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tgatgaagat gcgttagatg + 721 atgatgatga tgaatttatt tatccgaaca gctattaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL219 11219 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert PCR3909 AN% into Partial digest of p3909 +ACCESSION pUL219 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 11219) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: Partial digest of p3909 + Fragment ends: AscI and NcoI + Fragment size: 10538 + Insert molecule: Insert PCR3909 AN% + Fragment ends: NcoI and AscI + Fragment size: 708 +FEATURES Location/Qualifiers + CDS complement (146..406) + /gene="lacZ" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_feature 417..1441 + /gene="UF'" + /SECDrawAs="Region" + CDS 1458..3806 + /gene="rrm4" + /product="rrm4 ORF" + /SECDrawAs="Gene" + CDS 1458..4520 + /gene="Rrm4-MLLE3-αi,iiÃŽâ€-HA-3C-mKate2" + /SECDrawAs="Gene" + misc_feature 1676..1880 + /gene="RRM1" + /SECDrawAs="Region" + misc_feature 1928..2096 + /gene="RRM2" + /SECDrawAs="Region" + misc_feature 2423..2639 + /gene="RRM3" + /SECDrawAs="Region" + misc_feature 2808..3059 + /gene="MLLE1" + /SECDrawAs="Region" + misc_signal 2808..3059 + /gene="MLLE1" + /SECDrawAs="Label" + misc_feature 3177..3335 + /gene="MLLE2" + /SECDrawAs="Region" + misc_signal 3177..3335 + /gene="MLLE2" + /SECDrawAs="Label" + CDS 3492..3542 + /gene="HA-3C" + /SECDrawAs="Gene" + CDS 3492..3518 + /gene="HA" + /SECDrawAs="Gene" + misc_feature 3543..3806 + /gene="MLLE3" + /SECDrawAs="Region" + CDS 3543..3806 + /gene="MLLE3" + /SECDrawAs="Gene" + misc_signal 3807..3820 + /gene="Linker'" + /SECDrawAs="Label" + CDS 3822..4520 + /gene="mKate2" + /SECDrawAs="Gene" + misc_feature 4529..4822 + /gene="Tnos" + /product="nos-terminator" + /SECDrawAs="Region" + CDS 4827..7502 + /gene="HygR" + /product="Hygromycin resistance casette" + /SECDrawAs="Gene" + misc_feature 4829..5377 + /gene="hsp70T" + /SECDrawAs="Region" + misc_feature 6707..7502 + /gene="hsp70P" + /SECDrawAs="Region" + misc_feature 7530..8925 + /gene="DF" + /SECDrawAs="Region" + misc_signal 7682..7682 + /gene="G to C" + /product="mutated to remove NdeI" + /SECDrawAs="Label" + CDS 8136..8900 + /gene="cytochrome b561" + /product="UMAG_10835" + /SECDrawAs="Gene" + misc_signal 8929..8932 + /gene="'BsaI DF" + /SECDrawAs="Label" + CDS complement (8935..9002) + /gene="lacZ'" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 10303..10303 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (10159..11019) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" +ORIGIN + 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca + 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg + 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc + 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc + 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat + 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt + 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcacc tgcaatatta + 421 gtttcggggt catcatggga caaacatgca accggcgtga ggcttgcggt ttggggacat + 481 tcgtgattta cgatgcacga ttcacgattc acgattcacg attcacgatt cgtgatatct + 541 gattggagca ggtgcagtcc aagcgatcta acaaggagtt tatgcctcac cccttacctc + 601 tcacgcctcg ccgcacgcat ccacagtcca cgatcgtgca tagcagattt gtagagtcgt + 661 gtgttgaggt aaagacgttc acagtttcat gattcacaaa gctaacacga atcgtgaatc + 721 acattaacat cgttaagtag cagataatcg taattgtgaa tctgtgattt ggtttaagta + 781 cgtgcgaaaa cacataagtt acacactatc aacatcacaa acacggcctt tgtgacacgc + 841 aatggctacc acgacggcaa aattcacaga tttgatatct gtagcctatt gatgtggaca + 901 gagattgacc tcgatcttga tcttgacctg gtcaactttt gtggtcctct tacttgtgct + 961 tatcttgaac cttcatgagt gtggcaaggt ggcagaagga gggtgacatg ccctaggtgc + 1021 gctgaactct ccaccgcggc cacacgtgac accacgcagt gcgtgtgtta ctttcgcttc + 1081 ctttgagatc tttcttggaa gaacaagctc aagttgaggt tcacagaccc aaaaaccaaa + 1141 aaaaaaaaaa aatcgtgaat gaaaggcggc gtgtgcatgc gtgtgtattg catgacgatc + 1201 atttcaacgc tcgtcttgaa tcgtttgagt acgaagcggc atggcagctc gcactccgaa + 1261 agagactgtg caactcacta gagcccgtct tcggaagatc cgtctgcgac tctgctgccc + 1321 acctgtagtt tagcgtcgtg atcctcgaca ttgtctcacg accaagacga cgacaacaac + 1381 aacaacaacg acaacgtttc ggctcaagtc ggaaccttgc cgctctgctt taaaggtcat + 1441 cggcctagaa ggcccatatg tccgattcga tttacgcccc acataataag cacaagctcg + 1501 aggcagcgcg agccgctgat gctgccgccg atgatgccgc caccgtcagc gccctcgtcg + 1561 agccgaccga ctccactgcg caggcaagtc acgctgcgga gcaaaccatt gatgcgcatc + 1621 agcaagcagg tgacgtcgaa cctgaacgat gccatcctca cctcactcgc cccttgctct + 1681 accttagcgg tgtcgatgcc accatgactg acaaggagct cgccggcctt gtctttgatc + 1741 aagtactccc cgtccggctc aaaattgacc gcaccgtggg cgagggccag acagccagcg + 1801 gcaccgtcga gttccagacg ctggataagg ccgagaaggc ttatgctacc gttcgtcctc + 1861 cgatccagct gcgcatcaac caggacgcct ccatcagaga gccgcacccc tcggccaaac + 1921 cacgtcttgt caagcagctg ccacctacat cggacgacgc gttcgtatac gacctcttcc + 1981 gtcctttcgg tccgctccga cgagctcagt gtctgctcac caatccagcc ggcattcaca + 2041 ccggcttcaa gggtatggcc gtgcttgaat tctactctga acaagacgcc cagcgtgccg + 2101 aatccgagat gcactgcagc gaagtcggcg gcaagtcgat ctctgttgcc attgataccg + 2161 ctacgcgcaa ggtcagcgct gctgcagccg agttccgtcc ctcggctgct gcattcgttc + 2221 cagcgggcag catgagtccg tcagctccgt cctttgaccc ttaccctgct ggctctcgtt + 2281 ccgtttcgac aggctcagcc gcttccatct acgcaacttc tggcgccgct ccgactcacg + 2341 atacccgcaa cggtgctcag aagggagcgc gtgttccgct ccagtattcg agccaagcat + 2401 ctacgtatgt cgacccttgc aacctcttca tcaagaacct cgaccccaac atggaatcca + 2461 atgacctctt cgacaccttc aaaaggtttg gccacatcgt ctctgcacgc gtcatgcgcg + 2521 acgataacgg caagagcaga gaatttgggt ttgtcagctt caccactcct gacgaagcgc + 2581 aacaagctct acaagcaatg gacaatgcca aacttggcac caaaaagatc attgtccgtc + 2641 tgcacgagcc caagacgatg cgtcaggaga agctcgcagc gcgttacaac gctgccaatg + 2701 ccgataacag tgacatgagc agcaacagtc cgcccacaga ggctcgcaaa gccgacaagc + 2761 gtcaaagcag aagctacttc aaagcaggtg tgccttcgga tgccagcggt cttgtagacg + 2821 aagagcaatt gcgctcgctc agcactgtcg tgagaaacga gctcttgtct ggcgaattta + 2881 cacgaagaat ccccaaggtt tcctcggtca ccgaggcgca gttggacgac gttgtaggcg + 2941 agctgctcag tctcaagctt gctgatgccg tcgaggcgct caataaccca atttcgctta + 3001 ttcaaaggat cagcgatgct cgcgagcagc ttgcacagaa gtctgcctcc actctgacgg + 3061 ctccgagtcc ggcgccactg tctgctgaac acccagctat gctcggtatt caggctcagc + 3121 gtagcgtatc aagcgcatct tcgaccggcg aaggaggtgc gtcggtcaag gagagggaga + 3181 ggctactcaa ggctgtcatc tccgtcaccg aaagtggtgc ccccgttgag gacatcaccg + 3241 acatgatcgc aagcctgcca aagaaggatc gtgcccttgc gctcttcaat ccagagtttc + 3301 tcaagcaaaa agtggacgaa gccaaggaca ttctcgacat caccgacgaa agtggcgaag + 3361 atctttcgcc tcctcgtgct tccagtggga gcgctccggt gccattgtca gtgcagacgc + 3421 ctgcttctgc tattttcaaa gatgccagca acggccagag ctccatctcg cctggcgctg + 3481 ctgaggctta ctacccttac gacgtgcccg actacgccct ggaagtactg ttccaggggc + 3541 ccctccctaa agcggatcca gcgacggtca aggcaaccga cgatttcatc gactcgcttc + 3601 agggcaaagc agcgcacgat cagaagcaga agctgggtga tcagctcttc aaaaagatcc + 3661 gtacgttcgg cgtcaaaggc gcacccaagc tcaccatcca cttgttggat tccgaagact + 3721 tgcgggcgct ggcgcatctc atgaactcgt acgaggacgt gctcaaggaa aaagttcagc + 3781 acaaggttgc tgcaggtctg aacaaggcca acgcggccac catggtgtcg gagctcatca + 3841 aggagaacat gcacatgaag ctctacatgg agggcaccgt caacaaccac cacttcaagt + 3901 gcacctcgga aggcgagggc aagccctacg agggcaccca gaccatgcgc atcaaggccg + 3961 tcgagggtgg tcctctgccc ttcgccttcg acatcctcgc cacctcgttc atgtacggct + 4021 ccaagacgtt catcaaccac acgcagggca tccccgactt tttcaagcag tcgttccccg + 4081 agggcttcac ctgggagcgt gtcaccacct acgaggacgg cggtgtcctc accgccaccc + 4141 aggacacctc gctccaggac ggctgcctca tctacaacgt caagatccgc ggtgtcaact + 4201 tcccctcgaa cggtcccgtc atgcagaaga agacgctcgg ctgggaggcc tcgaccgaga + 4261 cgctctaccc cgccgacggt ggcctcgagg gtcgtgccga catggcgctc aagctcgtcg + 4321 gtggtggtca cctcatctgc aacctcaaga ccacctaccg ctccaagaag cccgccaaga + 4381 acctcaagat gcccggtgtc tactacgtcg accgtcgtct cgagcgcatc aaggaggccg + 4441 acaaggaaac ctacgtcgag cagcacgagg tcgccgtcgc tcgctactgc gatctgccct + 4501 cgaaactcgg tcaccgctaa accggtggcg cgccggccgc ccggcgatcg ttcaaacatt + 4561 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa + 4621 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg + 4681 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa + 4741 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatccg + 4801 atgataagct gtcaaacatg agaattgcgg ccgcactcga gtggcggcag atgtgagtcg + 4861 tgtgctacaa aacgtgaatc gacacgcgca gggcggaacc aaaaaaaacc cccaccccgc + 4921 tgtcaagttg acaaatcaac acatttgttc caagttcaaa tcgggaaatc aaaaattagg + 4981 gccagatcag cgatcaggaa tggtaatcgg taacagaggt cgcaaaatcg tctagaaagt + 5041 ggaagaagaa cgtggtaact accagcgagt tctgcaaact tcaaaaaaaa aatctgggca + 5101 cgatgaaagt tgagctaacg ctgacgctca caaatggcgt ggctaaagga agcgagacaa + 5161 tcggaaaatt gttctctcgg gcaccacaaa gctgttgtta gtcgctgaag aacaattcca + 5221 actgattccg ccgccttcct attgcgtcag ccttgtacct aagctgccga gtaacgtcac + 5281 tcaacctctc ttttcagact gctttgctcc gcgaatactt ttcttctatg cgctcaagaa + 5341 aatgacacag cacaccaagc tctgcaaact ttcttcgcta atctgacgcg aaatgtgagc + 5401 catttcttct cgcctgcaat ggcaatgcgt ctgtgcggcg atgagaatca cgatgcggaa + 5461 tgggtggctg gaagttcata gagatgctga gttgttggag cgacatggta cataagcatg + 5521 agtctgtcct gatttccacc ctcccgtctt tcatcaactt tctcgtctga cccttccgtt + 5581 gccagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga + 5641 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga + 5701 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag + 5761 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct + 5821 cccgattccg gaagtgcttg acattgggga actcagcgag agcctgacct attgcatctc + 5881 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct + 5941 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg + 6001 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg + 6061 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc + 6121 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg + 6181 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac + 6241 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat + 6301 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag + 6361 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga + 6421 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg + 6481 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag + 6541 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg + 6601 ccccagcact cgtccgaggg caaaggaata gagtagatgc cgaccgggat ctgggcagca + 6661 ccaagacacg gaacttcaac agtcgtgagt aggtggtgat ctgcatgtcc aaatcgtgaa + 6721 acgtgaatca cgaaccacga atcgtgaata gccactttgc gcgaaaatca ctgaatgccg + 6781 ccaaaccgac gcgacgcgag ttgcgtgaag tattcgtgat tcacattcac gattgacgat + 6841 tgtgagttgg tcgacgacat tcgctagcca gaccaacact cacgactttt tgaagagcgt + 6901 tgcaggctga gccaactttc tccctcactc tttgctttct tctccatcac gctcattcct + 6961 tttaagtgtt catccggtat cattagcact atatcatagt gaaactcgca acaacccggt + 7021 cttcgtcgcg aaagcaaccc tccatcgctg ctgcctcttg cagtgaagac cgccaccatg + 7081 cagtctacgc agtcgaagac cctacacgtc cagttcgtcc attctggtcc tacacattgt + 7141 atccacgcac ccaagtctgc caacgtggag taagtgaacc tttctttgtc ctgtcgaggc + 7201 tcaacgtaga tcacaggctg atcatccaca ctcttctacc ttttgacatt acgcagtcga + 7261 ctttttacag gaggagatga ctacctcgtc cgaatcttac ccaccctgcc tctctccgac + 7321 gtagagccgc agcttatcga agacgccact gaagcggtga cctcgctcga tgctgacggc + 7381 cgcttcctcg tcaccgcttc cgaagatggt gtcaacgcct ctaccgacac cacccaatcg + 7441 acgcggaagg caacccagca tcaccggctg tgcttcaatc gctgctgcgc cgcgaagctg + 7501 tgcggccgca ttaataggcc tgagtggccg aatcacgaat gtgcacattg tcactagggc + 7561 gtgcgtggta atgacacctt ccgcgtagtt ggttcgtgtt aacaagcgtt atataaaggt + 7621 cgaactggcg gccgctgtgg tccctagatc tctctgttct ccgagtgaca accagacata + 7681 tcttccactg ctaccgttgt aggattgtac caaggatgtg gagctgaggt aaagtgcggc + 7741 tatacacacg cttaatgtgc ggtgcagcaa gtcgtgattg ggctacacac gtggtcgcgg + 7801 cggatgagca agctccgaat cacgaatcag aatgtcgcaa tttgtgcgcg cgaatcacga + 7861 ttgcggtttt cacgtttttt gacaggcggc caagcagcca cgagattcac gattatctga + 7921 tttggtcagc atagacggca tggtatgtct ttcaacggac aggtttcgag tgtagcattt + 7981 cagcgcttgt tcgagtttac gtcgatcaac acgcacgctc tccactgtta tcgatggtcc + 8041 aaactagttc tgaggttctc tgagctgact ggtcgacatc gtagcggggt tccgaaggaa + 8101 agacttgctc tcacggcaca aacacaagcg aagccatgtt gcaaggtcgc gcgagcgagc + 8161 agggatccac cacgcaacca ctgctgacca ccacaaacga cgactaccac catcacgaga + 8221 cagacgcctt gatcacgccg ctcgacatgg ttcggcatga aaacgtctcg cgccatgcgg + 8281 tgggcgtgca agtttccgcg ttgctctttg tcgtcgttgt atggagcatt gttttctcca + 8341 ctatgtcgat cctttcgctg ccactcttcg gctatcatcc attgatccag tctttcacca + 8401 tactgcttct gctgcaggcg attgtagtac tgcagcggac gacgccatca caaccagcgg + 8461 cgaaaaaatc ggcgttcaca gcacaccagt ggatcaacct cattctcgta ctcccactct + 8521 tcaccgcggg cgcaagcatc atgtggtacc tgcacgatca accagcctcg gcacacttca + 8581 tatcgtacca tgggattctc ggtaccgccg tcgtaatcgc agcgtgggta caggccgcct + 8641 tcggtgccgc aagcgtctgg tggaagggaa agctagtggg tggagagaac aaaggtaagg + 8701 cgctttggaa gtggcataga ttgtctggct atgtactcat tgcgctgttt ctcttgacgg + 8761 ctgtgttggg tgtggtcgag actacgtggg ggaagcggaa tgcccggccg atccagaagg + 8821 gcgtgacggt ggtgacgtta agtttggcgg catttgcgat gctcatccgc gtgcaaaaga + 8881 gcaaactgcc aaagctgtag tcttccaacc gcgcttaggt gtaatattgc aggtctagat + 8941 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt aatcatggtc + 9001 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg + 9061 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt + 9121 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg + 9181 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga + 9241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat + 9301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca + 9361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc + 9421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata + 9481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc + 9541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc + 9601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga + 9661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc + 9721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag + 9781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag + 9841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag + 9901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca + 9961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga + 10021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat + 10081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga + 10141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg + 10201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga + 10261 gggcttacca tctggcccca gtgctgcaat gataccgcga gatccacgct caccggctcc + 10321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac + 10381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc + 10441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc + 10501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc + 10561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt + 10621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc + 10681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg + 10741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag + 10801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat + 10861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc + 10921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa + 10981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta + 11041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa + 11101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga + 11161 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc +// +LOCUS pUL220 11246 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert PCR3909 AN% into Partial digest of p3909 +ACCESSION pUL220 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 11246) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: Partial digest of p3909 + Fragment ends: AscI and NcoI + Fragment size: 10538 + Insert molecule: Insert PCR3909 AN% + Fragment ends: NcoI and AscI + Fragment size: 708 +FEATURES Location/Qualifiers + CDS complement (146..406) + /gene="lacZ" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_feature 417..1441 + /gene="UF'" + /SECDrawAs="Region" + CDS 1458..3833 + /gene="rrm4" + /product="rrm4 ORF" + /SECDrawAs="Gene" + CDS 1458..4547 + /gene="Rrm4-mKate2_HygR without HA tag" + /SECDrawAs="Gene" + misc_feature 1676..1880 + /gene="RRM1" + /SECDrawAs="Region" + misc_feature 1928..2096 + /gene="RRM2" + /SECDrawAs="Region" + misc_feature 2423..2639 + /gene="RRM3" + /SECDrawAs="Region" + misc_feature 2808..3059 + /gene="MLLE1" + /SECDrawAs="Region" + misc_signal 2808..3059 + /gene="MLLE1" + /SECDrawAs="Label" + misc_feature 3177..3335 + /gene="MLLE2" + /SECDrawAs="Region" + misc_signal 3177..3335 + /gene="MLLE2" + /SECDrawAs="Label" + misc_feature 3637..3833 + /gene="MLLE3 (PABC)" + /SECDrawAs="Region" + misc_feature 3654..3656 + /gene="Q733A" + /SECDrawAs="Region" + misc_signal 3654..3656 + /gene="Q733A" + /SECDrawAs="Label" + misc_feature 3675..3677 + /gene="F740A" + /SECDrawAs="Region" + misc_signal 3675..3677 + /gene="F740A" + /SECDrawAs="Label" + misc_signal 3834..3847 + /gene="Linker'" + /SECDrawAs="Label" + CDS 3849..4547 + /gene="mKate2" + /SECDrawAs="Gene" + misc_feature 4556..4849 + /gene="Tnos" + /product="nos-terminator" + /SECDrawAs="Region" + CDS 4854..7529 + /gene="HygR" + /product="Hygromycin resistance casette" + /SECDrawAs="Gene" + misc_feature 4856..5404 + /gene="hsp70T" + /SECDrawAs="Region" + misc_feature 6734..7529 + /gene="hsp70P" + /SECDrawAs="Region" + misc_feature 7557..8952 + /gene="DF" + /SECDrawAs="Region" + misc_signal 7709..7709 + /gene="G to C" + /product="mutated to remove NdeI" + /SECDrawAs="Label" + CDS 8163..8927 + /gene="cytochrome b561" + /product="UMAG_10835" + /SECDrawAs="Gene" + CDS 8953..416 + /gene="desire region" + /SECDrawAs="Gene" + misc_signal 8956..8959 + /gene="'BsaI DF" + /SECDrawAs="Label" + CDS complement (8962..9029) + /gene="lacZ'" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 10330..10330 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (10186..11046) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" +ORIGIN + 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca + 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg + 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc + 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc + 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat + 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt + 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcacc tgcaatatta + 421 gtttcggggt catcatggga caaacatgca accggcgtga ggcttgcggt ttggggacat + 481 tcgtgattta cgatgcacga ttcacgattc acgattcacg attcacgatt cgtgatatct + 541 gattggagca ggtgcagtcc aagcgatcta acaaggagtt tatgcctcac cccttacctc + 601 tcacgcctcg ccgcacgcat ccacagtcca cgatcgtgca tagcagattt gtagagtcgt + 661 gtgttgaggt aaagacgttc acagtttcat gattcacaaa gctaacacga atcgtgaatc + 721 acattaacat cgttaagtag cagataatcg taattgtgaa tctgtgattt ggtttaagta + 781 cgtgcgaaaa cacataagtt acacactatc aacatcacaa acacggcctt tgtgacacgc + 841 aatggctacc acgacggcaa aattcacaga tttgatatct gtagcctatt gatgtggaca + 901 gagattgacc tcgatcttga tcttgacctg gtcaactttt gtggtcctct tacttgtgct + 961 tatcttgaac cttcatgagt gtggcaaggt ggcagaagga gggtgacatg ccctaggtgc + 1021 gctgaactct ccaccgcggc cacacgtgac accacgcagt gcgtgtgtta ctttcgcttc + 1081 ctttgagatc tttcttggaa gaacaagctc aagttgaggt tcacagaccc aaaaaccaaa + 1141 aaaaaaaaaa aatcgtgaat gaaaggcggc gtgtgcatgc gtgtgtattg catgacgatc + 1201 atttcaacgc tcgtcttgaa tcgtttgagt acgaagcggc atggcagctc gcactccgaa + 1261 agagactgtg caactcacta gagcccgtct tcggaagatc cgtctgcgac tctgctgccc + 1321 acctgtagtt tagcgtcgtg atcctcgaca ttgtctcacg accaagacga cgacaacaac + 1381 aacaacaacg acaacgtttc ggctcaagtc ggaaccttgc cgctctgctt taaaggtcat + 1441 cggcctagaa ggcccatatg tccgattcga tttacgcccc acataataag cacaagctcg + 1501 aggcagcgcg agccgctgat gctgccgccg atgatgccgc caccgtcagc gccctcgtcg + 1561 agccgaccga ctccactgcg caggcaagtc acgctgcgga gcaaaccatt gatgcgcatc + 1621 agcaagcagg tgacgtcgaa cctgaacgat gccatcctca cctcactcgc cccttgctct + 1681 accttagcgg tgtcgatgcc accatgactg acaaggagct cgccggcctt gtctttgatc + 1741 aagtactccc cgtccggctc aaaattgacc gcaccgtggg cgagggccag acagccagcg + 1801 gcaccgtcga gttccagacg ctggataagg ccgagaaggc ttatgctacc gttcgtcctc + 1861 cgatccagct gcgcatcaac caggacgcct ccatcagaga gccgcacccc tcggccaaac + 1921 cacgtcttgt caagcagctg ccacctacat cggacgacgc gttcgtatac gacctcttcc + 1981 gtcctttcgg tccgctccga cgagctcagt gtctgctcac caatccagcc ggcattcaca + 2041 ccggcttcaa gggtatggcc gtgcttgaat tctactctga acaagacgcc cagcgtgccg + 2101 aatccgagat gcactgcagc gaagtcggcg gcaagtcgat ctctgttgcc attgataccg + 2161 ctacgcgcaa ggtcagcgct gctgcagccg agttccgtcc ctcggctgct gcattcgttc + 2221 cagcgggcag catgagtccg tcagctccgt cctttgaccc ttaccctgct ggctctcgtt + 2281 ccgtttcgac aggctcagcc gcttccatct acgcaacttc tggcgccgct ccgactcacg + 2341 atacccgcaa cggtgctcag aagggagcgc gtgttccgct ccagtattcg agccaagcat + 2401 ctacgtatgt cgacccttgc aacctcttca tcaagaacct cgaccccaac atggaatcca + 2461 atgacctctt cgacaccttc aaaaggtttg gccacatcgt ctctgcacgc gtcatgcgcg + 2521 acgataacgg caagagcaga gaatttgggt ttgtcagctt caccactcct gacgaagcgc + 2581 aacaagctct acaagcaatg gacaatgcca aacttggcac caaaaagatc attgtccgtc + 2641 tgcacgagcc caagacgatg cgtcaggaga agctcgcagc gcgttacaac gctgccaatg + 2701 ccgataacag tgacatgagc agcaacagtc cgcccacaga ggctcgcaaa gccgacaagc + 2761 gtcaaagcag aagctacttc aaagcaggtg tgccttcgga tgccagcggt cttgtagacg + 2821 aagagcaatt gcgctcgctc agcactgtcg tgagaaacga gctcttgtct ggcgaattta + 2881 cacgaagaat ccccaaggtt tcctcggtca ccgaggcgca gttggacgac gttgtaggcg + 2941 agctgctcag tctcaagctt gctgatgccg tcgaggcgct caataaccca atttcgctta + 3001 ttcaaaggat cagcgatgct cgcgagcagc ttgcacagaa gtctgcctcc actctgacgg + 3061 ctccgagtcc ggcgccactg tctgctgaac acccagctat gctcggtatt caggctcagc + 3121 gtagcgtatc aagcgcatct tcgaccggcg aaggaggtgc gtcggtcaag gagagggaga + 3181 ggctactcaa ggctgtcatc tccgtcaccg aaagtggtgc ccccgttgag gacatcaccg + 3241 acatgatcgc aagcctgcca aagaaggatc gtgcccttgc gctcttcaat ccagagtttc + 3301 tcaagcaaaa agtggacgaa gccaaggaca ttctcgacat caccgacgaa agtggcgaag + 3361 atctttcgcc tcctcgtgct tccagtggga gcgctccggt gccattgtca gtgcagacgc + 3421 ctgcttctgc tattttcaaa gatgccagca acggccagag ctccatctcg cctggcgctg + 3481 ctgaggctta cacattatcc acgcttgctg cgcttcctgc agccgagatt gtgcgcttgg + 3541 ccaactccca atccagctcc ggtctgcctc tccctaaagc ggatccagcg acggtcaagg + 3601 caaccgacga tttcatcgac tcgcttcagg gcaaagcagc gcacgatcag aaggcgaagc + 3661 tgggtgatca gctcgccaaa aagatccgta cgttcggcgt caaaggcgca cccaagctca + 3721 ccatccactt gttggattcc gaagacttgc gggcgctggc gcatctcatg aactcgtacg + 3781 aggacgtgct caaggaaaaa gttcagcaca aggttgctgc aggtctgaac aaggccaacg + 3841 cggccaccat ggtgtcggag ctcatcaagg agaacatgca catgaagctc tacatggagg + 3901 gcaccgtcaa caaccaccac ttcaagtgca cctcggaagg cgagggcaag ccctacgagg + 3961 gcacccagac catgcgcatc aaggccgtcg agggtggtcc tctgcccttc gccttcgaca + 4021 tcctcgccac ctcgttcatg tacggctcca agacgttcat caaccacacg cagggcatcc + 4081 ccgacttttt caagcagtcg ttccccgagg gcttcacctg ggagcgtgtc accacctacg + 4141 aggacggcgg tgtcctcacc gccacccagg acacctcgct ccaggacggc tgcctcatct + 4201 acaacgtcaa gatccgcggt gtcaacttcc cctcgaacgg tcccgtcatg cagaagaaga + 4261 cgctcggctg ggaggcctcg accgagacgc tctaccccgc cgacggtggc ctcgagggtc + 4321 gtgccgacat ggcgctcaag ctcgtcggtg gtggtcacct catctgcaac ctcaagacca + 4381 cctaccgctc caagaagccc gccaagaacc tcaagatgcc cggtgtctac tacgtcgacc + 4441 gtcgtctcga gcgcatcaag gaggccgaca aggaaaccta cgtcgagcag cacgaggtcg + 4501 ccgtcgctcg ctactgcgat ctgccctcga aactcggtca ccgctaaacc ggtggcgcgc + 4561 cggccgcccg gcgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt + 4621 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt + 4681 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta + 4741 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc + 4801 gcggtgtcat ctatgttact agatccgatg ataagctgtc aaacatgaga attgcggccg + 4861 cactcgagtg gcggcagatg tgagtcgtgt gctacaaaac gtgaatcgac acgcgcaggg + 4921 cggaaccaaa aaaaaccccc accccgctgt caagttgaca aatcaacaca tttgttccaa + 4981 gttcaaatcg ggaaatcaaa aattagggcc agatcagcga tcaggaatgg taatcggtaa + 5041 cagaggtcgc aaaatcgtct agaaagtgga agaagaacgt ggtaactacc agcgagttct + 5101 gcaaacttca aaaaaaaaat ctgggcacga tgaaagttga gctaacgctg acgctcacaa + 5161 atggcgtggc taaaggaagc gagacaatcg gaaaattgtt ctctcgggca ccacaaagct + 5221 gttgttagtc gctgaagaac aattccaact gattccgccg ccttcctatt gcgtcagcct + 5281 tgtacctaag ctgccgagta acgtcactca acctctcttt tcagactgct ttgctccgcg + 5341 aatacttttc ttctatgcgc tcaagaaaat gacacagcac accaagctct gcaaactttc + 5401 ttcgctaatc tgacgcgaaa tgtgagccat ttcttctcgc ctgcaatggc aatgcgtctg + 5461 tgcggcgatg agaatcacga tgcggaatgg gtggctggaa gttcatagag atgctgagtt + 5521 gttggagcga catggtacat aagcatgagt ctgtcctgat ttccaccctc ccgtctttca + 5581 tcaactttct cgtctgaccc ttccgttgcc agatcccggg gggcaatgag atatgaaaaa + 5641 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc + 5701 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg + 5761 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt + 5821 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaact + 5881 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct + 5941 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc + 6001 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca + 6061 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca + 6121 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct + 6181 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa + 6241 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg + 6301 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga + 6361 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg + 6421 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt + 6481 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac + 6541 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga + 6601 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag + 6661 tagatgccga ccgggatctg ggcagcacca agacacggaa cttcaacagt cgtgagtagg + 6721 tggtgatctg catgtccaaa tcgtgaaacg tgaatcacga accacgaatc gtgaatagcc + 6781 actttgcgcg aaaatcactg aatgccgcca aaccgacgcg acgcgagttg cgtgaagtat + 6841 tcgtgattca cattcacgat tgacgattgt gagttggtcg acgacattcg ctagccagac + 6901 caacactcac gactttttga agagcgttgc aggctgagcc aactttctcc ctcactcttt + 6961 gctttcttct ccatcacgct cattcctttt aagtgttcat ccggtatcat tagcactata + 7021 tcatagtgaa actcgcaaca acccggtctt cgtcgcgaaa gcaaccctcc atcgctgctg + 7081 cctcttgcag tgaagaccgc caccatgcag tctacgcagt cgaagaccct acacgtccag + 7141 ttcgtccatt ctggtcctac acattgtatc cacgcaccca agtctgccaa cgtggagtaa + 7201 gtgaaccttt ctttgtcctg tcgaggctca acgtagatca caggctgatc atccacactc + 7261 ttctaccttt tgacattacg cagtcgactt tttacaggag gagatgacta cctcgtccga + 7321 atcttaccca ccctgcctct ctccgacgta gagccgcagc ttatcgaaga cgccactgaa + 7381 gcggtgacct cgctcgatgc tgacggccgc ttcctcgtca ccgcttccga agatggtgtc + 7441 aacgcctcta ccgacaccac ccaatcgacg cggaaggcaa cccagcatca ccggctgtgc + 7501 ttcaatcgct gctgcgccgc gaagctgtgc ggccgcatta ataggcctga gtggccgaat + 7561 cacgaatgtg cacattgtca ctagggcgtg cgtggtaatg acaccttccg cgtagttggt + 7621 tcgtgttaac aagcgttata taaaggtcga actggcggcc gctgtggtcc ctagatctct + 7681 ctgttctccg agtgacaacc agacatatct tccactgcta ccgttgtagg attgtaccaa + 7741 ggatgtggag ctgaggtaaa gtgcggctat acacacgctt aatgtgcggt gcagcaagtc + 7801 gtgattgggc tacacacgtg gtcgcggcgg atgagcaagc tccgaatcac gaatcagaat + 7861 gtcgcaattt gtgcgcgcga atcacgattg cggttttcac gttttttgac aggcggccaa + 7921 gcagccacga gattcacgat tatctgattt ggtcagcata gacggcatgg tatgtctttc + 7981 aacggacagg tttcgagtgt agcatttcag cgcttgttcg agtttacgtc gatcaacacg + 8041 cacgctctcc actgttatcg atggtccaaa ctagttctga ggttctctga gctgactggt + 8101 cgacatcgta gcggggttcc gaaggaaaga cttgctctca cggcacaaac acaagcgaag + 8161 ccatgttgca aggtcgcgcg agcgagcagg gatccaccac gcaaccactg ctgaccacca + 8221 caaacgacga ctaccaccat cacgagacag acgccttgat cacgccgctc gacatggttc + 8281 ggcatgaaaa cgtctcgcgc catgcggtgg gcgtgcaagt ttccgcgttg ctctttgtcg + 8341 tcgttgtatg gagcattgtt ttctccacta tgtcgatcct ttcgctgcca ctcttcggct + 8401 atcatccatt gatccagtct ttcaccatac tgcttctgct gcaggcgatt gtagtactgc + 8461 agcggacgac gccatcacaa ccagcggcga aaaaatcggc gttcacagca caccagtgga + 8521 tcaacctcat tctcgtactc ccactcttca ccgcgggcgc aagcatcatg tggtacctgc + 8581 acgatcaacc agcctcggca cacttcatat cgtaccatgg gattctcggt accgccgtcg + 8641 taatcgcagc gtgggtacag gccgccttcg gtgccgcaag cgtctggtgg aagggaaagc + 8701 tagtgggtgg agagaacaaa ggtaaggcgc tttggaagtg gcatagattg tctggctatg + 8761 tactcattgc gctgtttctc ttgacggctg tgttgggtgt ggtcgagact acgtggggga + 8821 agcggaatgc ccggccgatc cagaagggcg tgacggtggt gacgttaagt ttggcggcat + 8881 ttgcgatgct catccgcgtg caaaagagca aactgccaaa gctgtagtct tccaaccgcg + 8941 cttaggtgta atattgcagg tctagatatc ggatcccggg cccgtcgact gcagaggcct + 9001 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct + 9061 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg + 9121 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct + 9181 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg + 9241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc + 9301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg + 9361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct + 9421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca + 9481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct + 9541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc + 9601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt + 9661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc + 9721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc + 9781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg + 9841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc + 9901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag + 9961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga + 10021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat + 10081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag + 10141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat + 10201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc + 10261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat + 10321 accgcgagat ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag + 10381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg + 10441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc + 10501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca + 10561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg + 10621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc + 10681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta + 10741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc + 10801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg + 10861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc + 10921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc + 10981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat + 11041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag + 11101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc + 11161 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa + 11221 taggcgtatc acgaggccct ttcgtc +// +LOCUS pUL221 9272 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert PCR3909 AN% into Partial digest of p3909 +ACCESSION pUL221 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 9272) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: Partial digest of p3909 + Fragment ends: AscI and NcoI + Fragment size: 10538 + Insert molecule: Insert PCR3909 AN% + Fragment ends: NcoI and AscI + Fragment size: 708 +FEATURES Location/Qualifiers + CDS complement (146..406) + /gene="lacZ" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_feature 417..1441 + /gene="UF'" + /SECDrawAs="Region" + CDS 1458..1859 + /gene="rrm4" + /product="rrm4 ORF" + /SECDrawAs="Gene" + CDS 1458..2573 + /gene="Rrm4-mKate2_HygR without HA tag" + /SECDrawAs="Gene" + CDS 1503..1517 + /gene="M3 overhang" + /SECDrawAs="Gene" + misc_feature 1518..1859 + /gene="MLLE3" + /SECDrawAs="Region" + CDS 1518..1859 + /gene="MLLE3" + /SECDrawAs="Gene" + CDS 1518..1595 + /gene="alpha I,II" + /SECDrawAs="Gene" + misc_signal 1860..1873 + /gene="Linker'" + /SECDrawAs="Label" + CDS 1875..2573 + /gene="mKate2" + /SECDrawAs="Gene" + misc_feature 2582..2875 + /gene="Tnos" + /product="nos-terminator" + /SECDrawAs="Region" + CDS 2880..5555 + /gene="HygR" + /product="Hygromycin resistance casette" + /SECDrawAs="Gene" + misc_feature 2882..3430 + /gene="hsp70T" + /SECDrawAs="Region" + misc_feature 4760..5555 + /gene="hsp70P" + /SECDrawAs="Region" + misc_feature 5583..6978 + /gene="DF" + /SECDrawAs="Region" + misc_signal 5735..5735 + /gene="G to C" + /product="mutated to remove NdeI" + /SECDrawAs="Label" + CDS 6189..6953 + /gene="cytochrome b561" + /product="UMAG_10835" + /SECDrawAs="Gene" + misc_signal 6982..6985 + /gene="'BsaI DF" + /SECDrawAs="Label" + CDS complement (6988..7055) + /gene="lacZ'" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 8356..8356 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (8212..9072) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" +ORIGIN + 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca + 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg + 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc + 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc + 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat + 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt + 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcacc tgcaatatta + 421 gtttcggggt catcatggga caaacatgca accggcgtga ggcttgcggt ttggggacat + 481 tcgtgattta cgatgcacga ttcacgattc acgattcacg attcacgatt cgtgatatct + 541 gattggagca ggtgcagtcc aagcgatcta acaaggagtt tatgcctcac cccttacctc + 601 tcacgcctcg ccgcacgcat ccacagtcca cgatcgtgca tagcagattt gtagagtcgt + 661 gtgttgaggt aaagacgttc acagtttcat gattcacaaa gctaacacga atcgtgaatc + 721 acattaacat cgttaagtag cagataatcg taattgtgaa tctgtgattt ggtttaagta + 781 cgtgcgaaaa cacataagtt acacactatc aacatcacaa acacggcctt tgtgacacgc + 841 aatggctacc acgacggcaa aattcacaga tttgatatct gtagcctatt gatgtggaca + 901 gagattgacc tcgatcttga tcttgacctg gtcaactttt gtggtcctct tacttgtgct + 961 tatcttgaac cttcatgagt gtggcaaggt ggcagaagga gggtgacatg ccctaggtgc + 1021 gctgaactct ccaccgcggc cacacgtgac accacgcagt gcgtgtgtta ctttcgcttc + 1081 ctttgagatc tttcttggaa gaacaagctc aagttgaggt tcacagaccc aaaaaccaaa + 1141 aaaaaaaaaa aatcgtgaat gaaaggcggc gtgtgcatgc gtgtgtattg catgacgatc + 1201 atttcaacgc tcgtcttgaa tcgtttgagt acgaagcggc atggcagctc gcactccgaa + 1261 agagactgtg caactcacta gagcccgtct tcggaagatc cgtctgcgac tctgctgccc + 1321 acctgtagtt tagcgtcgtg atcctcgaca ttgtctcacg accaagacga cgacaacaac + 1381 aacaacaacg acaacgtttc ggctcaagtc ggaaccttgc cgctctgctt taaaggtcat + 1441 cggcctagaa ggcccatatg tccgattcga tttacgcccc acataataag cacaagctcg + 1501 aggctgctga ggcttacaca ttatccacgc ttgctgcgct tcctgcagcc gagattgtgc + 1561 gcttggccaa ctcccaatcc agctccggtc tgcctctccc taaagcggat ccagcgacgg + 1621 tcaaggcaac cgacgatttc atcgactcgc ttcagggcaa agcagcgcac gatcagaagc + 1681 agaagctggg tgatcagctc ttcaaaaaga tccgtacgtt cggcgtcaaa ggcgcaccca + 1741 agctcaccat ccacttgttg gattccgaag acttgcgggc gctggcgcat ctcatgaact + 1801 cgtacgagga cgtgctcaag gaaaaagttc agcacaaggt tgctgcaggt ctgaacaagg + 1861 ccaacgcggc caccatggtg tcggagctca tcaaggagaa catgcacatg aagctctaca + 1921 tggagggcac cgtcaacaac caccacttca agtgcacctc ggaaggcgag ggcaagccct + 1981 acgagggcac ccagaccatg cgcatcaagg ccgtcgaggg tggtcctctg cccttcgcct + 2041 tcgacatcct cgccacctcg ttcatgtacg gctccaagac gttcatcaac cacacgcagg + 2101 gcatccccga ctttttcaag cagtcgttcc ccgagggctt cacctgggag cgtgtcacca + 2161 cctacgagga cggcggtgtc ctcaccgcca cccaggacac ctcgctccag gacggctgcc + 2221 tcatctacaa cgtcaagatc cgcggtgtca acttcccctc gaacggtccc gtcatgcaga + 2281 agaagacgct cggctgggag gcctcgaccg agacgctcta ccccgccgac ggtggcctcg + 2341 agggtcgtgc cgacatggcg ctcaagctcg tcggtggtgg tcacctcatc tgcaacctca + 2401 agaccaccta ccgctccaag aagcccgcca agaacctcaa gatgcccggt gtctactacg + 2461 tcgaccgtcg tctcgagcgc atcaaggagg ccgacaagga aacctacgtc gagcagcacg + 2521 aggtcgccgt cgctcgctac tgcgatctgc cctcgaaact cggtcaccgc taaaccggtg + 2581 gcgcgccggc cgcccggcga tcgttcaaac atttggcaat aaagtttctt aagattgaat + 2641 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta + 2701 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg + 2761 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta + 2821 tcgcgcgcgg tgtcatctat gttactagat ccgatgataa gctgtcaaac atgagaattg + 2881 cggccgcact cgagtggcgg cagatgtgag tcgtgtgcta caaaacgtga atcgacacgc + 2941 gcagggcgga accaaaaaaa acccccaccc cgctgtcaag ttgacaaatc aacacatttg + 3001 ttccaagttc aaatcgggaa atcaaaaatt agggccagat cagcgatcag gaatggtaat + 3061 cggtaacaga ggtcgcaaaa tcgtctagaa agtggaagaa gaacgtggta actaccagcg + 3121 agttctgcaa acttcaaaaa aaaaatctgg gcacgatgaa agttgagcta acgctgacgc + 3181 tcacaaatgg cgtggctaaa ggaagcgaga caatcggaaa attgttctct cgggcaccac + 3241 aaagctgttg ttagtcgctg aagaacaatt ccaactgatt ccgccgcctt cctattgcgt + 3301 cagccttgta cctaagctgc cgagtaacgt cactcaacct ctcttttcag actgctttgc + 3361 tccgcgaata cttttcttct atgcgctcaa gaaaatgaca cagcacacca agctctgcaa + 3421 actttcttcg ctaatctgac gcgaaatgtg agccatttct tctcgcctgc aatggcaatg + 3481 cgtctgtgcg gcgatgagaa tcacgatgcg gaatgggtgg ctggaagttc atagagatgc + 3541 tgagttgttg gagcgacatg gtacataagc atgagtctgt cctgatttcc accctcccgt + 3601 ctttcatcaa ctttctcgtc tgacccttcc gttgccagat cccggggggc aatgagatat + 3661 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag + 3721 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt + 3781 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg + 3841 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg + 3901 ggaactcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca + 3961 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc + 4021 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat + 4081 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca + 4141 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct + 4201 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc + 4261 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat + 4321 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg + 4381 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg + 4441 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg + 4501 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc + 4561 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg + 4621 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga + 4681 atagagtaga tgccgaccgg gatctgggca gcaccaagac acggaacttc aacagtcgtg + 4741 agtaggtggt gatctgcatg tccaaatcgt gaaacgtgaa tcacgaacca cgaatcgtga + 4801 atagccactt tgcgcgaaaa tcactgaatg ccgccaaacc gacgcgacgc gagttgcgtg + 4861 aagtattcgt gattcacatt cacgattgac gattgtgagt tggtcgacga cattcgctag + 4921 ccagaccaac actcacgact ttttgaagag cgttgcaggc tgagccaact ttctccctca + 4981 ctctttgctt tcttctccat cacgctcatt ccttttaagt gttcatccgg tatcattagc + 5041 actatatcat agtgaaactc gcaacaaccc ggtcttcgtc gcgaaagcaa ccctccatcg + 5101 ctgctgcctc ttgcagtgaa gaccgccacc atgcagtcta cgcagtcgaa gaccctacac + 5161 gtccagttcg tccattctgg tcctacacat tgtatccacg cacccaagtc tgccaacgtg + 5221 gagtaagtga acctttcttt gtcctgtcga ggctcaacgt agatcacagg ctgatcatcc + 5281 acactcttct accttttgac attacgcagt cgacttttta caggaggaga tgactacctc + 5341 gtccgaatct tacccaccct gcctctctcc gacgtagagc cgcagcttat cgaagacgcc + 5401 actgaagcgg tgacctcgct cgatgctgac ggccgcttcc tcgtcaccgc ttccgaagat + 5461 ggtgtcaacg cctctaccga caccacccaa tcgacgcgga aggcaaccca gcatcaccgg + 5521 ctgtgcttca atcgctgctg cgccgcgaag ctgtgcggcc gcattaatag gcctgagtgg + 5581 ccgaatcacg aatgtgcaca ttgtcactag ggcgtgcgtg gtaatgacac cttccgcgta + 5641 gttggttcgt gttaacaagc gttatataaa ggtcgaactg gcggccgctg tggtccctag + 5701 atctctctgt tctccgagtg acaaccagac atatcttcca ctgctaccgt tgtaggattg + 5761 taccaaggat gtggagctga ggtaaagtgc ggctatacac acgcttaatg tgcggtgcag + 5821 caagtcgtga ttgggctaca cacgtggtcg cggcggatga gcaagctccg aatcacgaat + 5881 cagaatgtcg caatttgtgc gcgcgaatca cgattgcggt tttcacgttt tttgacaggc + 5941 ggccaagcag ccacgagatt cacgattatc tgatttggtc agcatagacg gcatggtatg + 6001 tctttcaacg gacaggtttc gagtgtagca tttcagcgct tgttcgagtt tacgtcgatc + 6061 aacacgcacg ctctccactg ttatcgatgg tccaaactag ttctgaggtt ctctgagctg + 6121 actggtcgac atcgtagcgg ggttccgaag gaaagacttg ctctcacggc acaaacacaa + 6181 gcgaagccat gttgcaaggt cgcgcgagcg agcagggatc caccacgcaa ccactgctga + 6241 ccaccacaaa cgacgactac caccatcacg agacagacgc cttgatcacg ccgctcgaca + 6301 tggttcggca tgaaaacgtc tcgcgccatg cggtgggcgt gcaagtttcc gcgttgctct + 6361 ttgtcgtcgt tgtatggagc attgttttct ccactatgtc gatcctttcg ctgccactct + 6421 tcggctatca tccattgatc cagtctttca ccatactgct tctgctgcag gcgattgtag + 6481 tactgcagcg gacgacgcca tcacaaccag cggcgaaaaa atcggcgttc acagcacacc + 6541 agtggatcaa cctcattctc gtactcccac tcttcaccgc gggcgcaagc atcatgtggt + 6601 acctgcacga tcaaccagcc tcggcacact tcatatcgta ccatgggatt ctcggtaccg + 6661 ccgtcgtaat cgcagcgtgg gtacaggccg ccttcggtgc cgcaagcgtc tggtggaagg + 6721 gaaagctagt gggtggagag aacaaaggta aggcgctttg gaagtggcat agattgtctg + 6781 gctatgtact cattgcgctg tttctcttga cggctgtgtt gggtgtggtc gagactacgt + 6841 gggggaagcg gaatgcccgg ccgatccaga agggcgtgac ggtggtgacg ttaagtttgg + 6901 cggcatttgc gatgctcatc cgcgtgcaaa agagcaaact gccaaagctg tagtcttcca + 6961 accgcgctta ggtgtaatat tgcaggtcta gatatcggat cccgggcccg tcgactgcag + 7021 aggcctgcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta + 7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc + 7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg + 7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg + 7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg + 7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa + 7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc + 7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc + 7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag + 7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct + 7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta + 7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc + 7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc + 7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt + 7861 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct + 7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc + 7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca + 8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta + 8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa + 8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg + 8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg + 8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc + 8341 aatgataccg cgagatccac gctcaccggc tccagattta tcagcaataa accagccagc + 8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa + 8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc + 8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg + 8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc + 8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat + 8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg + 8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc + 8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg + 8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat + 8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg + 9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg + 9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct + 9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac + 9181 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta + 9241 taaaaatagg cgtatcacga ggccctttcg tc +// +LOCUS pUL222 11246 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert PCR3909 AN% into Partial digest of p3909 +ACCESSION pUL222 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 11246) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: Partial digest of p3909 + Fragment ends: AscI and NcoI + Fragment size: 10538 + Insert molecule: Insert PCR3909 AN% + Fragment ends: NcoI and AscI + Fragment size: 708 +FEATURES Location/Qualifiers + CDS complement (146..406) + /gene="lacZ" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_feature 417..1441 + /gene="UF'" + /SECDrawAs="Region" + CDS 1458..3833 + /gene="rrm4" + /product="rrm4 ORF" + /SECDrawAs="Gene" + CDS 1458..4547 + /gene="Rrm4-mKate2_HygR without HA tag" + /SECDrawAs="Gene" + CDS 1503..3476 + /gene="Rrm4-AA-16-673" + /SECDrawAs="Gene" + misc_feature 1676..1880 + /gene="RRM1" + /SECDrawAs="Region" + misc_feature 1928..2096 + /gene="RRM2" + /SECDrawAs="Region" + misc_feature 2423..2639 + /gene="RRM3" + /SECDrawAs="Region" + misc_feature 2808..3059 + /gene="MLLE1" + /SECDrawAs="Region" + misc_signal 2808..3059 + /gene="MLLE1" + /SECDrawAs="Label" + misc_feature 3177..3335 + /gene="MLLE2" + /SECDrawAs="Region" + misc_signal 3177..3335 + /gene="MLLE2" + /SECDrawAs="Label" + CDS 3477..3491 + /gene="M3 overhang" + /SECDrawAs="Gene" + misc_feature 3492..3833 + /gene="MLLE3" + /SECDrawAs="Region" + CDS 3492..3833 + /gene="MLLE3" + /SECDrawAs="Gene" + CDS 3492..3569 + /gene="alpha I,II" + /SECDrawAs="Gene" + CDS 3654..3656 + /gene="Q733A" + /SECDrawAs="Gene" + misc_signal 3654..3656 + /gene="(null)" + /SECDrawAs="Label" + misc_signal 3834..3847 + /gene="Linker'" + /SECDrawAs="Label" + CDS 3849..4547 + /gene="mKate2" + /SECDrawAs="Gene" + misc_feature 4556..4849 + /gene="Tnos" + /product="nos-terminator" + /SECDrawAs="Region" + CDS 4854..7529 + /gene="HygR" + /product="Hygromycin resistance casette" + /SECDrawAs="Gene" + misc_feature 4856..5404 + /gene="hsp70T" + /SECDrawAs="Region" + misc_feature 6734..7529 + /gene="hsp70P" + /SECDrawAs="Region" + misc_feature 7557..8952 + /gene="DF" + /SECDrawAs="Region" + misc_signal 7709..7709 + /gene="G to C" + /product="mutated to remove NdeI" + /SECDrawAs="Label" + CDS 8163..8927 + /gene="cytochrome b561" + /product="UMAG_10835" + /SECDrawAs="Gene" + misc_signal 8956..8959 + /gene="'BsaI DF" + /SECDrawAs="Label" + CDS complement (8962..9029) + /gene="lacZ'" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 10330..10330 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (10186..11046) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" +ORIGIN + 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca + 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg + 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc + 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc + 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat + 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt + 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcacc tgcaatatta + 421 gtttcggggt catcatggga caaacatgca accggcgtga ggcttgcggt ttggggacat + 481 tcgtgattta cgatgcacga ttcacgattc acgattcacg attcacgatt cgtgatatct + 541 gattggagca ggtgcagtcc aagcgatcta acaaggagtt tatgcctcac cccttacctc + 601 tcacgcctcg ccgcacgcat ccacagtcca cgatcgtgca tagcagattt gtagagtcgt + 661 gtgttgaggt aaagacgttc acagtttcat gattcacaaa gctaacacga atcgtgaatc + 721 acattaacat cgttaagtag cagataatcg taattgtgaa tctgtgattt ggtttaagta + 781 cgtgcgaaaa cacataagtt acacactatc aacatcacaa acacggcctt tgtgacacgc + 841 aatggctacc acgacggcaa aattcacaga tttgatatct gtagcctatt gatgtggaca + 901 gagattgacc tcgatcttga tcttgacctg gtcaactttt gtggtcctct tacttgtgct + 961 tatcttgaac cttcatgagt gtggcaaggt ggcagaagga gggtgacatg ccctaggtgc + 1021 gctgaactct ccaccgcggc cacacgtgac accacgcagt gcgtgtgtta ctttcgcttc + 1081 ctttgagatc tttcttggaa gaacaagctc aagttgaggt tcacagaccc aaaaaccaaa + 1141 aaaaaaaaaa aatcgtgaat gaaaggcggc gtgtgcatgc gtgtgtattg catgacgatc + 1201 atttcaacgc tcgtcttgaa tcgtttgagt acgaagcggc atggcagctc gcactccgaa + 1261 agagactgtg caactcacta gagcccgtct tcggaagatc cgtctgcgac tctgctgccc + 1321 acctgtagtt tagcgtcgtg atcctcgaca ttgtctcacg accaagacga cgacaacaac + 1381 aacaacaacg acaacgtttc ggctcaagtc ggaaccttgc cgctctgctt taaaggtcat + 1441 cggcctagaa ggcccatatg tccgattcga tttacgcccc acataataag cacaagctcg + 1501 aggcagcgcg agccgctgat gctgccgccg atgatgccgc caccgtcagc gccctcgtcg + 1561 agccgaccga ctccactgcg caggcaagtc acgctgcgga gcaaaccatt gatgcgcatc + 1621 agcaagcagg tgacgtcgaa cctgaacgat gccatcctca cctcactcgc cccttgctct + 1681 accttagcgg tgtcgatgcc accatgactg acaaggagct cgccggcctt gtctttgatc + 1741 aagtactccc cgtccggctc aaaattgacc gcaccgtggg cgagggccag acagccagcg + 1801 gcaccgtcga gttccagacg ctggataagg ccgagaaggc ttatgctacc gttcgtcctc + 1861 cgatccagct gcgcatcaac caggacgcct ccatcagaga gccgcacccc tcggccaaac + 1921 cacgtcttgt caagcagctg ccacctacat cggacgacgc gttcgtatac gacctcttcc + 1981 gtcctttcgg tccgctccga cgagctcagt gtctgctcac caatccagcc ggcattcaca + 2041 ccggcttcaa gggtatggcc gtgcttgaat tctactctga acaagacgcc cagcgtgccg + 2101 aatccgagat gcactgcagc gaagtcggcg gcaagtcgat ctctgttgcc attgataccg + 2161 ctacgcgcaa ggtcagcgct gctgcagccg agttccgtcc ctcggctgct gcattcgttc + 2221 cagcgggcag catgagtccg tcagctccgt cctttgaccc ttaccctgct ggctctcgtt + 2281 ccgtttcgac aggctcagcc gcttccatct acgcaacttc tggcgccgct ccgactcacg + 2341 atacccgcaa cggtgctcag aagggagcgc gtgttccgct ccagtattcg agccaagcat + 2401 ctacgtatgt cgacccttgc aacctcttca tcaagaacct cgaccccaac atggaatcca + 2461 atgacctctt cgacaccttc aaaaggtttg gccacatcgt ctctgcacgc gtcatgcgcg + 2521 acgataacgg caagagcaga gaatttgggt ttgtcagctt caccactcct gacgaagcgc + 2581 aacaagctct acaagcaatg gacaatgcca aacttggcac caaaaagatc attgtccgtc + 2641 tgcacgagcc caagacgatg cgtcaggaga agctcgcagc gcgttacaac gctgccaatg + 2701 ccgataacag tgacatgagc agcaacagtc cgcccacaga ggctcgcaaa gccgacaagc + 2761 gtcaaagcag aagctacttc aaagcaggtg tgccttcgga tgccagcggt cttgtagacg + 2821 aagagcaatt gcgctcgctc agcactgtcg tgagaaacga gctcttgtct ggcgaattta + 2881 cacgaagaat ccccaaggtt tcctcggtca ccgaggcgca gttggacgac gttgtaggcg + 2941 agctgctcag tctcaagctt gctgatgccg tcgaggcgct caataaccca atttcgctta + 3001 ttcaaaggat cagcgatgct cgcgagcagc ttgcacagaa gtctgcctcc actctgacgg + 3061 ctccgagtcc ggcgccactg tctgctgaac acccagctat gctcggtatt caggctcagc + 3121 gtagcgtatc aagcgcatct tcgaccggcg aaggaggtgc gtcggtcaag gagagggaga + 3181 ggctactcaa ggctgtcatc tccgtcaccg aaagtggtgc ccccgttgag gacatcaccg + 3241 acatgatcgc aagcctgcca aagaaggatc gtgcccttgc gctcttcaat ccagagtttc + 3301 tcaagcaaaa agtggacgaa gccaaggaca ttctcgacat caccgacgaa agtggcgaag + 3361 atctttcgcc tcctcgtgct tccagtggga gcgctccggt gccattgtca gtgcagacgc + 3421 ctgcttctgc tattttcaaa gatgccagca acggccagag ctccatctcg cctggcgctg + 3481 ctgaggctta cacattatcc acgcttgctg cgcttcctgc agccgagatt gtgcgcttgg + 3541 ccaactccca atccagctcc ggtctgcctc tccctaaagc ggatccagcg acggtcaagg + 3601 caaccgacga tttcatcgac tcgcttcagg gcaaagcagc gcacgatcag aaggcgaagc + 3661 tgggtgatca gctcttcaaa aagatccgta cgttcggcgt caaaggcgca cccaagctca + 3721 ccatccactt gttggattcc gaagacttgc gggcgctggc gcatctcatg aactcgtacg + 3781 aggacgtgct caaggaaaaa gttcagcaca aggttgctgc aggtctgaac aaggccaacg + 3841 cggccaccat ggtgtcggag ctcatcaagg agaacatgca catgaagctc tacatggagg + 3901 gcaccgtcaa caaccaccac ttcaagtgca cctcggaagg cgagggcaag ccctacgagg + 3961 gcacccagac catgcgcatc aaggccgtcg agggtggtcc tctgcccttc gccttcgaca + 4021 tcctcgccac ctcgttcatg tacggctcca agacgttcat caaccacacg cagggcatcc + 4081 ccgacttttt caagcagtcg ttccccgagg gcttcacctg ggagcgtgtc accacctacg + 4141 aggacggcgg tgtcctcacc gccacccagg acacctcgct ccaggacggc tgcctcatct + 4201 acaacgtcaa gatccgcggt gtcaacttcc cctcgaacgg tcccgtcatg cagaagaaga + 4261 cgctcggctg ggaggcctcg accgagacgc tctaccccgc cgacggtggc ctcgagggtc + 4321 gtgccgacat ggcgctcaag ctcgtcggtg gtggtcacct catctgcaac ctcaagacca + 4381 cctaccgctc caagaagccc gccaagaacc tcaagatgcc cggtgtctac tacgtcgacc + 4441 gtcgtctcga gcgcatcaag gaggccgaca aggaaaccta cgtcgagcag cacgaggtcg + 4501 ccgtcgctcg ctactgcgat ctgccctcga aactcggtca ccgctaaacc ggtggcgcgc + 4561 cggccgcccg gcgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt + 4621 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt + 4681 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta + 4741 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc + 4801 gcggtgtcat ctatgttact agatccgatg ataagctgtc aaacatgaga attgcggccg + 4861 cactcgagtg gcggcagatg tgagtcgtgt gctacaaaac gtgaatcgac acgcgcaggg + 4921 cggaaccaaa aaaaaccccc accccgctgt caagttgaca aatcaacaca tttgttccaa + 4981 gttcaaatcg ggaaatcaaa aattagggcc agatcagcga tcaggaatgg taatcggtaa + 5041 cagaggtcgc aaaatcgtct agaaagtgga agaagaacgt ggtaactacc agcgagttct + 5101 gcaaacttca aaaaaaaaat ctgggcacga tgaaagttga gctaacgctg acgctcacaa + 5161 atggcgtggc taaaggaagc gagacaatcg gaaaattgtt ctctcgggca ccacaaagct + 5221 gttgttagtc gctgaagaac aattccaact gattccgccg ccttcctatt gcgtcagcct + 5281 tgtacctaag ctgccgagta acgtcactca acctctcttt tcagactgct ttgctccgcg + 5341 aatacttttc ttctatgcgc tcaagaaaat gacacagcac accaagctct gcaaactttc + 5401 ttcgctaatc tgacgcgaaa tgtgagccat ttcttctcgc ctgcaatggc aatgcgtctg + 5461 tgcggcgatg agaatcacga tgcggaatgg gtggctggaa gttcatagag atgctgagtt + 5521 gttggagcga catggtacat aagcatgagt ctgtcctgat ttccaccctc ccgtctttca + 5581 tcaactttct cgtctgaccc ttccgttgcc agatcccggg gggcaatgag atatgaaaaa + 5641 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc + 5701 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg + 5761 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt + 5821 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaact + 5881 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct + 5941 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc + 6001 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca + 6061 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca + 6121 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct + 6181 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa + 6241 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg + 6301 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga + 6361 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg + 6421 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt + 6481 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac + 6541 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga + 6601 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag + 6661 tagatgccga ccgggatctg ggcagcacca agacacggaa cttcaacagt cgtgagtagg + 6721 tggtgatctg catgtccaaa tcgtgaaacg tgaatcacga accacgaatc gtgaatagcc + 6781 actttgcgcg aaaatcactg aatgccgcca aaccgacgcg acgcgagttg cgtgaagtat + 6841 tcgtgattca cattcacgat tgacgattgt gagttggtcg acgacattcg ctagccagac + 6901 caacactcac gactttttga agagcgttgc aggctgagcc aactttctcc ctcactcttt + 6961 gctttcttct ccatcacgct cattcctttt aagtgttcat ccggtatcat tagcactata + 7021 tcatagtgaa actcgcaaca acccggtctt cgtcgcgaaa gcaaccctcc atcgctgctg + 7081 cctcttgcag tgaagaccgc caccatgcag tctacgcagt cgaagaccct acacgtccag + 7141 ttcgtccatt ctggtcctac acattgtatc cacgcaccca agtctgccaa cgtggagtaa + 7201 gtgaaccttt ctttgtcctg tcgaggctca acgtagatca caggctgatc atccacactc + 7261 ttctaccttt tgacattacg cagtcgactt tttacaggag gagatgacta cctcgtccga + 7321 atcttaccca ccctgcctct ctccgacgta gagccgcagc ttatcgaaga cgccactgaa + 7381 gcggtgacct cgctcgatgc tgacggccgc ttcctcgtca ccgcttccga agatggtgtc + 7441 aacgcctcta ccgacaccac ccaatcgacg cggaaggcaa cccagcatca ccggctgtgc + 7501 ttcaatcgct gctgcgccgc gaagctgtgc ggccgcatta ataggcctga gtggccgaat + 7561 cacgaatgtg cacattgtca ctagggcgtg cgtggtaatg acaccttccg cgtagttggt + 7621 tcgtgttaac aagcgttata taaaggtcga actggcggcc gctgtggtcc ctagatctct + 7681 ctgttctccg agtgacaacc agacatatct tccactgcta ccgttgtagg attgtaccaa + 7741 ggatgtggag ctgaggtaaa gtgcggctat acacacgctt aatgtgcggt gcagcaagtc + 7801 gtgattgggc tacacacgtg gtcgcggcgg atgagcaagc tccgaatcac gaatcagaat + 7861 gtcgcaattt gtgcgcgcga atcacgattg cggttttcac gttttttgac aggcggccaa + 7921 gcagccacga gattcacgat tatctgattt ggtcagcata gacggcatgg tatgtctttc + 7981 aacggacagg tttcgagtgt agcatttcag cgcttgttcg agtttacgtc gatcaacacg + 8041 cacgctctcc actgttatcg atggtccaaa ctagttctga ggttctctga gctgactggt + 8101 cgacatcgta gcggggttcc gaaggaaaga cttgctctca cggcacaaac acaagcgaag + 8161 ccatgttgca aggtcgcgcg agcgagcagg gatccaccac gcaaccactg ctgaccacca + 8221 caaacgacga ctaccaccat cacgagacag acgccttgat cacgccgctc gacatggttc + 8281 ggcatgaaaa cgtctcgcgc catgcggtgg gcgtgcaagt ttccgcgttg ctctttgtcg + 8341 tcgttgtatg gagcattgtt ttctccacta tgtcgatcct ttcgctgcca ctcttcggct + 8401 atcatccatt gatccagtct ttcaccatac tgcttctgct gcaggcgatt gtagtactgc + 8461 agcggacgac gccatcacaa ccagcggcga aaaaatcggc gttcacagca caccagtgga + 8521 tcaacctcat tctcgtactc ccactcttca ccgcgggcgc aagcatcatg tggtacctgc + 8581 acgatcaacc agcctcggca cacttcatat cgtaccatgg gattctcggt accgccgtcg + 8641 taatcgcagc gtgggtacag gccgccttcg gtgccgcaag cgtctggtgg aagggaaagc + 8701 tagtgggtgg agagaacaaa ggtaaggcgc tttggaagtg gcatagattg tctggctatg + 8761 tactcattgc gctgtttctc ttgacggctg tgttgggtgt ggtcgagact acgtggggga + 8821 agcggaatgc ccggccgatc cagaagggcg tgacggtggt gacgttaagt ttggcggcat + 8881 ttgcgatgct catccgcgtg caaaagagca aactgccaaa gctgtagtct tccaaccgcg + 8941 cttaggtgta atattgcagg tctagatatc ggatcccggg cccgtcgact gcagaggcct + 9001 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct + 9061 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg + 9121 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct + 9181 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg + 9241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc + 9301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg + 9361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct + 9421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca + 9481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct + 9541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc + 9601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt + 9661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc + 9721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc + 9781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg + 9841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc + 9901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag + 9961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga + 10021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat + 10081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag + 10141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat + 10201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc + 10261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat + 10321 accgcgagat ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag + 10381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg + 10441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc + 10501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca + 10561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg + 10621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc + 10681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta + 10741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc + 10801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg + 10861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc + 10921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc + 10981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat + 11041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag + 11101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc + 11161 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa + 11221 taggcgtatc acgaggccct ttcgtc +// +LOCUS pUL224 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert pUL0224 into pUMa3262_pET22b_VBB-NdeI,XhoI% +ACCESSION pUL224 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: pUMa3262_pET22b_VBB-NdeI,XhoI% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: Insert pUL0224 + Fragment ends: NdeI and XhoI + Fragment size: 413 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..701 + /gene="pET22_n6His_TS_SUMO" + /SECDrawAs="Gene" + CDS 345..758 + /gene="pET22_n6His_TS_SUMO_PAM2 of UMAG-10620" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="PAM2L of Um_Taf7" + /SECDrawAs="Gene" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="bla A - AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg tggtggtggt ggtaaaggtt + 721 tcaacatcga cgacttcatc tacccgcacg gtatctaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL226 5781 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert pUL0226 into pUMa3262_pET22b_VBB-NdeI,XhoI% +ACCESSION pUL226 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5781) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: pUMa3262_pET22b_VBB-NdeI,XhoI% + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: Insert pUL0226 + Fragment ends: NdeI and XhoI + Fragment size: 413 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + source 344..701 + /gene="''source'" + /SECDrawAs="Info only" + CDS 345..701 + /gene="pET22_n6His_TS_SUMO" + /SECDrawAs="Gene" + CDS 345..758 + /gene="pET22_n6His_TS_SUMO_PAM2 of UMAG_15064" + /SECDrawAs="Gene" + CDS 357..374 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 384..401 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 384..401 + /gene="Thrombine site" + /SECDrawAs="Region" + CDS 408..701 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + misc_signal 408..701 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 702..755 + /gene="PAM2L2 of Vps8" + /SECDrawAs="Gene" + terminator 854..895 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1520..2377 + /gene="bla A - AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4572..5651) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc + 361 atcatcatca tcacggcagc ggcctggtgc cgcgcggcag cgctagcatg tcggactcag + 421 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca + 481 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt + 541 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat + 601 tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg + 661 ataacgatat tattgaggct cacagagaac agattggtgg taccgaccag gcgccgtctg + 721 acgactcttt ctctttccgt tacccgcacc cgctgtaact cgagcaccac caccaccacc + 781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg + 841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga + 901 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc + 961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc + 1021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct + 1081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa + 1141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc + 1201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact + 1261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg + 1321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt + 1381 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt + 1441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata + 1501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt + 1561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc + 1621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat + 1681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct + 1741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca + 1801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg + 1861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa + 1921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg + 1981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga + 2041 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg + 2101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt + 2161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg + 2221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc + 2281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca + 2341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc + 2401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat + 2461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc + 2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg + 2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct + 2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct + 2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct + 2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg + 2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc + 2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga + 2941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg + 3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta + 3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg + 3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg + 3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat + 3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc + 3301 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg + 3361 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta + 3421 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc + 3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag + 3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg + 3601 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct + 3661 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa + 3721 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg + 3781 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg + 3841 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat + 3901 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag + 3961 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg + 4021 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc + 4081 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta + 4141 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg + 4201 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct + 4261 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc + 4321 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga + 4381 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca + 4441 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg + 4501 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg + 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa + 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc + 4681 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc + 4741 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc + 4801 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca + 4861 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca + 4921 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg + 4981 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga + 5041 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac + 5101 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct + 5161 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc + 5221 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta + 5281 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg + 5341 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat + 5401 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca + 5461 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc + 5521 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc + 5581 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt + 5641 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg + 5701 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc + 5761 ctgcattagg aagcagccca g +// +LOCUS pUL234 6604 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert-ubr5% into r-pUL0253_GST-TEV-BsaI% +ACCESSION pUL234 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 6604) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: r-pUL0253_GST-TEV-BsaI% + Fragment ends: BsaI + Fragment size: 6371 + Insert molecule: insert-ubr5% + Fragment ends: BsaI + Fragment size: 233 +FEATURES Location/Qualifiers + misc_feature 370..386 + /gene="T7P" + /SECDrawAs="Region" + CDS 457..1389 + /gene="ORF" + /SECDrawAs="Gene" + CDS 457..1389 + /gene="GST-Ubr5-MLLE" + /SECDrawAs="Gene" + CDS 466..1113 + /gene="GST" + /product="glutathione S-transferase from Schistosoma + japonicum" + /SECDrawAs="Gene" + CDS 1123..1143 + /gene="TEV site" + /product="tobacco etch virus (TEV) protease recognition + and cleavage site" + /SECDrawAs="Gene" + CDS 1159..1389 + /gene="Ubr5-MLLE" + /SECDrawAs="Gene" + misc_feature 1400..1404 + /gene="MCS" + /product="multiple cloning site" + /SECDrawAs="Region" + misc_feature 1490..1531 + /gene="T7T" + /SECDrawAs="Region" + CDS 1953..2813 + /gene="AmpR" + /product="beta-lactamase" + /SECDrawAs="Gene" + misc_feature 3758..3900 + /gene="bom" + /product="basis of mobility region from pBR322" + /SECDrawAs="Region" + CDS complement (4002..4193) + /gene="rop" + /product="Rop protein, which maintains plasmids at low + copy number" + /SECDrawAs="Gene" + CDS complement (5505..6587) + /gene="lacI" + /product="lac repressor" + /SECDrawAs="Gene" +ORIGIN + 1 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt + 61 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg + 121 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac + 181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg + 241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca + 301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc + 361 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag + 421 aaataatttt gtttaacttt aagaaggaga tataccatgg gttcttcccc tatactaggt + 481 tattggaaaa ttaagggcct tgtgcaaccc actcgacttc ttttggaata tcttgaagaa + 541 aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt + 601 gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt taaattaaca + 661 cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg tggttgtcca + 721 aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag atacggtgtt + 781 tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct tagcaagcta + 841 cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt aaatggtgat + 901 catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac + 961 ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc + 1021 ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg + 1081 caagccacgt ttggtggtgg cgaccatcct ccaagcagcg gcgaaaacct gtacttccag + 1141 agcggcagcc atatgaccat gggcccccta ccagcacaca ggcaagctct aggagagcgc + 1201 ctgtacccgc gtgttcaggc aatgcagccg gcgttcgctt ccaaaatcac cggtatgctg + 1261 ctggaactga gcccggcgca actgttattg ttgttggcga gcgaagactc tctgcgtgca + 1321 cgcgtggacg aggctatgga actgattatc gcccacggcc gtgagaacgg tgcggatagc + 1381 attctctaac tcgagtaact cgagcgagct ctgcagcccg ggatccggct gctaacaaag + 1441 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg + 1501 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc + 1561 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac + 1621 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta + 1681 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg + 1741 agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga + 1801 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta + 1861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat + 1921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc + 1981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga + 2041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca + 2101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt + 2161 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg + 2221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc + 2281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata + 2341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt + 2401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag + 2461 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca + 2521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg + 2581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg + 2641 ctgataaatc tggagccggt gagcgtggct ctcgcggtat cattgcagca ctggggccag + 2701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg + 2761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag + 2821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga + 2881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt + 2941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc + 3001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc + 3061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac + 3121 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac + 3181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt + 3241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct + 3301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat + 3361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt + 3421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg + 3481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt + 3541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt + 3601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg + 3661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg + 3721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta + 3781 cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga + 3841 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg + 3901 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc + 3961 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca + 4021 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca + 4081 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc + 4141 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc + 4201 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg + 4261 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa + 4321 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc + 4381 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc + 4441 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa + 4501 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac + 4561 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc + 4621 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc + 4681 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg + 4741 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg + 4801 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac + 4861 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc + 4921 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg + 4981 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta + 5041 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa + 5101 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg + 5161 cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac + 5221 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga + 5281 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca + 5341 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc + 5401 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc + 5461 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc + 5521 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg + 5581 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga + 5641 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc + 5701 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg + 5761 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg + 5821 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg + 5881 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc + 5941 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc + 6001 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat + 6061 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg + 6121 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc + 6181 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc + 6241 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac + 6301 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac + 6361 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc + 6421 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt + 6481 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa + 6541 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg + 6601 aatt +// +LOCUS pUL235 5284 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Fragment Pab1-Hs into Fragment VBB +ACCESSION pUL235 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5284) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: Fragment VBB + Fragment ends: XhoI and NcoI + Fragment size: 4997 + Insert molecule: Fragment Pab1-Hs + Fragment ends: NcoI and XhoI + Fragment size: 287 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 295..1290 + /gene="GST-HsPABPC1-MLLE" + /SECDrawAs="Gene" + misc_signal 955..967 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 967..1004 + /gene="GS linker'" + /SECDrawAs="Gene" + CDS 1005..1291 + /gene="Hs_Pab1-MLLE" + /SECDrawAs="Gene" + CDS 1729..2589 + /gene="AmpR" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccggtggagg ttctggcggt ggatcgggag gttccatggg cttaacagcg + 1021 tcaatgctag ctagtgcacc cccacaagag cagaagcaaa tgctgggtga acgcctgttc + 1081 ccgctgattc aggctatgca cccgacgctg gcaggcaaaa tcaccggcat gttgttggag + 1141 atcgacaaca gcgaactgtt acacatgctt gagtccccgg aaagcctgcg tagcaaagtt + 1201 gatgaagcgg tggccgtgct gcaagcgcat caggcgaagg aggcggcaca gaaggcggtc + 1261 aattctgcta ccggtgttcc gaccgtttaa ctcgaggcca acgcggccta gtgaattcat + 1321 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac + 1381 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc + 1441 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt + 1501 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta + 1561 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat + 1621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg + 1681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa + 1741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac + 1801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac + 1861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt + 1921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc + 1981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca + 2041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc + 2101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag + 2161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa + 2221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg + 2281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa + 2341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg + 2401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt + 2461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt + 2521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag + 2581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat + 2641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct + 2701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct + 2761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca + 2821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc + 2881 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc + 2941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct + 3001 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag + 3061 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc + 3121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg + 3181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag + 3241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt + 3301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac + 3361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg + 3421 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc + 3481 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg + 3541 cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc cgacaccatc + 3601 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg + 3661 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat + 3721 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa + 3781 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg + 3841 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg + 3901 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg + 3961 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa + 4021 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa + 4081 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac + 4141 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg + 4201 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt + 4261 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa + 4321 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc + 4381 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt + 4441 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa + 4501 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg + 4561 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag + 4621 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc + 4681 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa + 4741 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc + 4801 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca + 4861 cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg + 4921 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca + 4981 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga + 5041 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg + 5101 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt + 5161 acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc + 5221 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac + 5281 agga +// +LOCUS pUL254 6379 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of 1712 MfeI, NdeI into product +ACCESSION pUL254 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 6379) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: product + Fragment ends: MfeI and NdeI + Fragment size: 1878 + Insert molecule: 1712 MfeI, NdeI + Fragment ends: NdeI and MfeI + Fragment size: 5015 +FEATURES Location/Qualifiers + misc_feature 120..379 + /gene="tefP" + /SECDrawAs="Region" + misc_signal 214..215 + /gene="a-deletion- gAc" + /SECDrawAs="Label" + misc_signal 291..291 + /gene="t-insertion" + /SECDrawAs="Label" + CDS 427..1542 + /gene="pRrm4-ÃŽâ€16-673AA-MLLE3-mKate2" + /SECDrawAs="Gene" + CDS 427..828 + /gene="pRrm4-ÃŽâ€16-673AA-MLLE3" + /SECDrawAs="Gene" + CDS 844..1542 + /gene="mKate2" + /SECDrawAs="Gene" + misc_signal 1545..1843 + /gene="nosT" + /SECDrawAs="Label" + misc_feature 1545..1843 + /gene="1st nosT" + /SECDrawAs="Region" + misc_feature 1845..2141 + /gene="2nd nosT" + /product="relict of pUMa962; new nosT comes from pUMa647 + along with triple Gfp" + /SECDrawAs="Region" + misc_signal 1846..2140 + /gene="zweiter nosT" + /product="sollte aber nicht stören" + /SECDrawAs="Label" + misc_feature 3206..4066 + /gene="amp" + /SECDrawAs="Region" + misc_feature 4836..5723 + /gene="cbx" + /SECDrawAs="Region" + CDS 4836..5723 + /gene="cbxR" + /SECDrawAs="Gene" +ORIGIN + 1 catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata + 61 cacatacgat ttaggtgaca ctatagaact cgagcagctg aagcttgtta attaagctcg + 121 agcagctgaa gctattcgac caatgaggcg cgagacgagg ggacgctgga agttgaggcg + 181 caagaaaatt tttctctggt tctgcgcggc agagcgacca gattcgcccg ctttcttctg + 241 cgttgggtgc ctcttttggg tgccagactt tgtgtgtgcg ccagcgagac tgttccaata + 301 aagggcgctg tctcggcact atctttcttt ctttcctcat acatcgtatc ataccataca + 361 cagacaacat catccacggg atccactagt aacggccgcc agtgtgctgg aattcgccct + 421 ttagccatgt ccgattcgat ttacgcccca cataataagc acaagctcga ggctgctgag + 481 gcttacacat tatccacgct tgctgcgctt cctgcagccg agattgtgcg cttggccaac + 541 tcccaatcca gctccggtct gcctctccct aaagcggatc cagcgacggt caaggcaacc + 601 gacgatttca tcgactcgct tcagggcaaa gcagcgcacg atcagaagca gaagctgggt + 661 gatcagctct tcaaaaagat ccgtacgttc ggcgtcaaag gcgcacccaa gctcaccatc + 721 cacttgttgg attccgaaga cttgcgggcg ctggcgcatc tcatgaactc gtacgaggac + 781 gtgctcaagg aaaaagttca gcacaaggtt gctgcaggtc tgaacaaggc caacgcggcc + 841 accatggtgt cggagctcat caaggagaac atgcacatga agctctacat ggagggcacc + 901 gtcaacaacc accacttcaa gtgcacctcg gaaggcgagg gcaagcccta cgagggcacc + 961 cagaccatgc gcatcaaggc cgtcgagggt ggtcctctgc ccttcgcctt cgacatcctc + 1021 gccacctcgt tcatgtacgg ctccaagacg ttcatcaacc acacgcaggg catccccgac + 1081 tttttcaagc agtcgttccc cgagggcttc acctgggagc gtgtcaccac ctacgaggac + 1141 ggcggtgtcc tcaccgccac ccaggacacc tcgctccagg acggctgcct catctacaac + 1201 gtcaagatcc gcggtgtcaa cttcccctcg aacggtcccg tcatgcagaa gaagacgctc + 1261 ggctgggagg cctcgaccga gacgctctac cccgccgacg gtggcctcga gggtcgtgcc + 1321 gacatggcgc tcaagctcgt cggtggtggt cacctcatct gcaacctcaa gaccacctac + 1381 cgctccaaga agcccgccaa gaacctcaag atgcccggtg tctactacgt cgaccgtcgt + 1441 ctcgagcgca tcaaggaggc cgacaaggaa acctacgtcg agcagcacga ggtcgccgtc + 1501 gctcgctact gcgatctgcc ctcgaaactc ggtcaccgct aaggcgcgcc ggccgcccgg + 1561 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc + 1621 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg + 1681 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata + 1741 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc + 1801 tatgttacta gatccgatga taagctgtca aacatgagaa ttgcggccgc ccggctgcag + 1861 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga + 1921 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca + 1981 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg + 2041 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta + 2101 tgttactaga tccgatgata agctgtcaaa catgagaatt cttatcgatg atatcagatc + 2161 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat + 2221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc + 2281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg + 2341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag + 2401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc + 2461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag + 2521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga + 2581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc + 2641 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg + 2701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt + 2761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca + 2821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca + 2881 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag + 2941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca + 3001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg + 3061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa + 3121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta + 3181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag + 3241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga + 3301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac + 3361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc + 3421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta + 3481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac + 3541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat + 3601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa + 3661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg + 3721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag + 3781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc + 3841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct + 3901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat + 3961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg + 4021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc + 4081 aatatcgagc acgttgatgg gatcgatcct agaatatcaa tcgagccacc gtcaatttcg + 4141 agcgctaagt ggagttgtcc gagtccccga atcacaagaa ttaggctcgt gctctgtgag + 4201 atctctcgcg aaacccagat gaaggaaaaa aatcggaaga tcgcggaaga agtggggttc + 4261 gcatggtcta acattgtcgc attcctcaca gtttcggctg gaaacggcag ggacaatcac + 4321 gagaaatgcg cgacgagtat tcagttgtcc aaaattatgt cagcttgaaa gttggaaacg + 4381 gcggaagaaa attcgtcagg agggcgttgc ggctagggtg aagaacggag acaatttcgg + 4441 ctttgaaatt ggctcgactc tgattggatc atgacgcact gctggtccac aaccgtgtcg + 4501 aaggtgccgt ctttactaca ggtccgctgg aagcaaaatg gaaaaagccg ctggagcccg + 4561 actacagagc cgccgtgttt tggtaatcag tcggcaaata ggtcagcaca gcgcagcgtg + 4621 acaggttctt gcaatttaca gcacagctcg tccgtctacg actttgcaca ccacaaagtg + 4681 tgcggggagc aaaggagccg atcttggtcg cgcgcaaagc caaggagtct tgaacctgag + 4741 agtgtgcgtg tcttgtgacg cttgcccttc tgtactttgc tgtgacacta ccaccacatc + 4801 tgtcttggct ttttgttcat acatccacac cgaccatgtc gctattcaac gtcagcaacg + 4861 gtcttcgtac cgctctccga ccttctgttg ccagctcttc gcgcgttgct gccttttcca + 4921 caaccgccgc tgcccgtctc gccacaccca cctctgacaa cgttggcagt tcgggcaagc + 4981 ctcagcactt gaagcagttc aagatctacc gatggaaccc tgacaagccc tcggagaagc + 5041 ctcgtctgca gtcgtacaca ctggacctca accagaccgg tccaatggtt ctcgacgcgc + 5101 tcatcaaaat caagaacgaa attgacccta cgctcacctt ccgtcgctcg tgccgtgagg + 5161 gtatctgcgg ttcgtgcgct atgaatattg acggtgtcaa caccctcgcc tgcctctgcc + 5221 ggatcgacaa gcagaatgac accaagatct accccttgcc gcacatgtac attgtcaagg + 5281 acctcgtgcc agacttgacc cagttctaca agcagtaccg atccatcgag cctttcctca + 5341 agtccaacaa caccccttct gagggtgaac atcttcagtc gcccgaggag cgtcgtcgac + 5401 tcgacggtct gtacgagtgc attctgtgcg cgtgctgctc cacatcctgc ccctcttact + 5461 ggtggaatca ggacgagtac cttggccccg ccgtgctcat gcaggcgtac cgatggatgg + 5521 ccgactcgcg tgacgacttt ggtgaggagc gaagacagaa gctcgagaac accttttcgc + 5581 tctaccgatg ccttaccatc atgaactgct ccaggacctg ccccaagaac ctcaaccctg + 5641 gtaaggcaat tgcacagatc aagaaggaca tggccgtcgg cgcacccaag gcttccgagc + 5701 gccctatcat ggcttcgtcg taatcttgat atatcatatc gttctttcct cagcacttct + 5761 tttgtcaatt tcaaaagtat ctaattgcat tcaactccgc ttgtggtttg ttgttcagtg + 5821 agagtggaaa cgctacgggc aagatgaggg cagtgttctg gcgacggaaa agtgtgcaag + 5881 tgtctggcct gcgtcctcgc tggttccagc agccgatgca ggacgtgtac ctagcgattt + 5941 cttcgacagc ctattgtggc agccgcgatt cgccacaatc gtacgaaagc gagacgagtt + 6001 gagcgaagat ccccattatt gaagcattta tcagggttat tgtctcatga gcggatacat + 6061 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt + 6121 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat + 6181 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca + 6241 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca + 6301 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca + 6361 gattgtactg agagtgcac +// +LOCUS pUL351 5263 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Gel eluted -Insert for pUL0130 into VBB - p3699% +ACCESSION pUL351 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5263) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB - p3699% + Fragment ends: XhoI and NdeI + Fragment size: 5006 + Insert molecule: Gel eluted -Insert for pUL0130 + Fragment ends: NdeI and XhoI + Fragment size: 350 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 295..1269 + /gene="GST_RiRrm4-MLLE3" + /SECDrawAs="Gene" + misc_signal 955..967 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 967..1013 + /gene="GS linker" + /SECDrawAs="Gene" + CDS 1018..1269 + /gene="Ri_Rrm4_MLLE3_573to655AA" + /SECDrawAs="Gene" + CDS 1708..2568 + /gene="AmpR" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccggtggagg ttctggcggt ggatcgggag gttccatggc acatatgccc + 1021 gagtacaagg agatcgagga gttcatggag gcgctcaaga acaagcccat ccacgagcag + 1081 aagcagaagc tcggcgaccg tctcttcccg aaagtcaagt cgttgggtct gaaatctgcc + 1141 atcgcatcga aggtgaccat caacctgctc gacacggatg atctccgtga gctggcgcac + 1201 tctatgaacg atacagagaa atttcgacaa aaggtcgctg cggcagcaga gaaggttgtg + 1261 cccaagtgac tcgaggccaa cgcggcctag tgaattcatc gtgactgact gacgatctgc + 1321 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc + 1381 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt + 1441 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat + 1501 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata + 1561 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt + 1621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg + 1681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt + 1741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta + 1801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc + 1861 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa + 1921 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc + 1981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt + 2041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact + 2101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac + 2161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata + 2221 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta + 2281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg + 2341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat + 2401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt + 2461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga + 2521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa + 2581 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag + 2641 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac + 2701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc + 2761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat + 2821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat + 2881 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct + 2941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt + 3001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg + 3061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta + 3121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg + 3181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg + 3241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc + 3301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg + 3361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat + 3421 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc + 3481 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat + 3541 ctgtgcggta tttcacaccg cataaattcc gacaccatcg aatggtgcaa aacctttcgc + 3601 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta + 3661 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg + 3721 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag + 3781 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt + 3841 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa + 3901 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc + 3961 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt + 4021 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg + 4081 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa + 4141 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg + 4201 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat + 4261 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc + 4321 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt + 4381 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt + 4441 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg + 4501 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg + 4561 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg + 4621 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat + 4681 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc + 4741 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc + 4801 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca + 4861 tgattacgga ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta + 4921 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg + 4981 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct + 5041 ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg + 5101 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca + 5161 acgtaaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt + 5221 gttactcgct cacatttaat gttgatgaaa gctggctaca gga +// +LOCUS pUL374 14999 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of SfiI casset into Fragment (Vps8) UMAG_15064 +ACCESSION pUL374 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 14999) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: Fragment (Vps8) + Fragment ends: SfiI + Fragment size: 11267 + Insert molecule: SfiI casset + Fragment ends: SfiI + Fragment size: 3733 +FEATURES Location/Qualifiers + misc_signal 1..24 + /gene="(null)" + /SECDrawAs="Label" + misc_feature 1..1016 + /gene="DF" + /SECDrawAs="Region" + CDS complement (161..856) + /gene="UMAG_11502" + /SECDrawAs="Gene" + misc_signal 991..1016 + /gene="(null)" + /SECDrawAs="Label" + misc_signal 1014..1016 + /gene="SspI 1st half" + /SECDrawAs="Label" + CDS complement (1022..1089) + /gene="lacZ'" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 2390..2390 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (2246..3106) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" + misc_signal 3697..3736 + /gene="(null)" + /SECDrawAs="Label" + CDS complement (3452..3712) + /gene="lacZ" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 3723..3725 + /gene="SspI 2nd half" + /SECDrawAs="Label" + misc_feature 3723..4737 + /gene="UF" + /SECDrawAs="Region" + CDS complement (3723..3944) + /gene="UMAG_02910'" + /SECDrawAs="Gene" + CDS 4738..5895 + /gene="Exon1-Vps8" + /SECDrawAs="Gene" + misc_signal 4780..5766 + /gene="NT deletion in pUL404" + /SECDrawAs="Label" + misc_feature 4780..5766 + /gene="NTD" + /product="NT Deletion in pUL404" + /SECDrawAs="Region" + misc_signal 5896..6034 + /gene="(null)" + /SECDrawAs="Label" + misc_feature 5896..6034 + /gene="intron" + /SECDrawAs="Region" + CDS 6035..11251 + /gene="Exon2-Vps8" + /SECDrawAs="Gene" + CDS 6035..11989 + /gene="Exon2-egfp" + /SECDrawAs="Gene" + CDS 11270..11989 + /gene="egfp" + /SECDrawAs="Gene" + misc_feature 11990..12292 + /gene="nosT" + /SECDrawAs="Region" + misc_feature complement (12299..12847) + /gene="hsp70T" + /product="hsp70 terminator" + /SECDrawAs="Region" + CDS complement (12848..14176) + /gene="hygR (hph)" + /SECDrawAs="Gene" + misc_feature complement (14177..14972) + /gene="P-hsp70" + /product="hsp70 promoter" + /SECDrawAs="Region" +ORIGIN + 1 ttcttgcaca tagtgattct ttgctcagtg tgattcttgc atgtgcgcaa cccattcatg + 61 tatccagatc tctgtacgct gaagatttgt gctgatgcga acgtcatttt gctttgcgtc + 121 aagtatgatt gtgagatggt cgcgtgatag agccgaagga tcaagaggat ttggcgttga + 181 cctcagcagg taaatcggca gcgacagccg cggcggtagc atcggctgtc gagggtgtgg + 241 tctgcgtacc gaatccacct gtcgacccaa accctccagc acccctaaga gtctcgctca + 301 agctttcaac ttcttgaacg tcgggtgtcg agatcttctc aaggatcaac tgagcgatgc + 361 ggtcaccttg gttaactgtt tcgttggcag accgcgttga tgtcagctcg gatcggttgc + 421 atgttgcgcg caaacaagaa tcgcacttac tgatgaagtc ttgttcgccc aagttgaaca + 481 gcaagacgcc gagcaagcca cggtagtctt ggtcgatcac accggctccc gtgtcaatgc + 541 cgtgtttgct cgcaagccct gaacgcggtg caacacggcc gtagtgtccg tcgggaatcg + 601 cgacgcagat accggtctga accaccttgc gaccgccttg tggaagcacc actttttcgg + 661 cagagtacag atcgtagccg gccgcaagcg ccgacccgcg cgtaggcagc ttggcgagcg + 721 gatgcgaagg gtgacgctgc accagcagcc gcactgtaga gttgacgctc atcgtaccgg + 781 cctcttcgag cttggcgagt ttcgtaggtc gagcttcgtc gcttgtacga gcgtcagagc + 841 agatgttcga cgtcatgaga gaaagcgaac gagcacgaac tggtatcgtc aggatcaacg + 901 gggcaaaggt gaaccatgaa ccgtgaacgc gttcgccacg agtgaatgtg ttattctctc + 961 gcccagtcac aatcaccaat cacgaattca cgattctcgg cgcgtgtcgc gtaaatattg + 1021 tctagatatc ggatcccagg cccgtcgact gcagaggcct gcatgcaagc ttggcgtaat + 1081 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac + 1141 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa + 1201 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat + 1261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc + 1321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg + 1381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag + 1441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc + 1501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag + 1561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga + 1621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc + 1681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg + 1741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt + 1801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca + 1861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca + 1921 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag + 1981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca + 2041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg + 2101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa + 2161 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta + 2221 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag + 2281 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga + 2341 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagat ccacgctcac + 2401 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc + 2461 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta + 2521 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac + 2581 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat + 2641 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa + 2701 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg + 2761 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag + 2821 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc + 2881 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct + 2941 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat + 3001 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg + 3061 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc + 3121 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta + 3181 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg + 3241 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct + 3301 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga + 3361 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag + 3421 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga + 3481 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca + 3541 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt + 3601 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc + 3661 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcacctgca + 3721 atattgggca agcaaggagt gaagtcatcc ggatgaccgt caagagcaaa gtggagcgtt + 3781 tggtgaatgg catgcgcgag ctctccatcg gccgatggtt tcagcgatct tgatacgtcg + 3841 tgcgtgtgaa tgtacaacga gcgaaagccg gacttgttgt ccgttagcgg acaagcagat + 3901 gctgttctag ctgttgttgc cgttgttgcc gttgctgagg tcatattgtg tgtggtggta + 3961 tggcaagctg tgtcaaggtg tcgcagagca aagccgagaa cagcacgcct aattctcatc + 4021 ttttgtcaaa ttacgactcg agccaagcac gaagcaagcg agtcagtatc acgattagtg + 4081 aatcagtgat tcgtgattcg aaagcggcaa tacaagccat gtggctccgt cattcagccc + 4141 ttccgaggag tgccgtcgct cggttccgtc acgcgttaaa cccacatgtg cccgtgccca + 4201 gtagttacgt attgttagca gcgtgaagca taacatgtgt gtataatatc cgtgatccgt + 4261 gatccacgat acacaatcac gaatcgtgaa tcactcgtga ctgaatatcg tgaatatcgt + 4321 gaatcgccaa tcgtgaatga gagtgtaact cacgactcca cgactcgtga ctgtcatgat + 4381 atttgtgatt catacaatca cgaagaacgc acggcctttc gtatttctct ctgtgttgcc + 4441 gcttttcacc gattttcgtc ctcgcaacgc catcgattgc aagtttgcga tagccgacat + 4501 cagtcgtcga ccatttcttg cctcccgcaa agtagtggcc gaagcagttg gccatccgat + 4561 tgcagcgcgg atctgcttta ccaacgactc tgcaccacta ggcacagcgg tccagcaacc + 4621 ccccaccatg tccaattgca tacgctctcg ctctcgcacc atcagatagg tgtcgtcaac + 4681 agcaccgcca ctatcacccc cgttcatcat catcattccc gctgcagcgt cttcggcatg + 4741 gaacacaaag actctgctga cttgaatcct tggggtgctg agccgtcgta tgctcagcgc + 4801 ctctcgtcct atcaggccga acaggatgct gactaccatc aacagctgga cgacctcctc + 4861 aattcgccca aagcatcccg atttagcgtt ccgcatgacg ccagcaccaa caacggcact + 4921 ttcctcaacg gtaatcatgt gcaccaccaa gacgacgaca atgacaacga cgacgacttt + 4981 gtctacgatg gtatcgatgc agcagctgcg ccttctgcat ggacgcccga cgatagcacc + 5041 gtctcgagcg aaatgagctc ggatacgtac agcgccaagc tcaaagacat cctcggcagc + 5101 gatgatgacg acgccggtcg agaccaacat gctgatctca cgattgaacg gcctgctcgc + 5161 tcctcgatca cctctccgtc ctcaccgaca actcagaagg cacccgtact ccaagttgat + 5221 gccgacacaa gttccaacac aacacctcgc catacagatc aagcccccag tgacgatagt + 5281 ttctccttca gatacccaca tccgctaccc aacgatgtca gcttcgacac cagatcatct + 5341 acctcatccc agcgccaccg aatcccatct cagccgtcat ctttgcgcat cttgacgccc + 5401 agatccactt ctggcatcgg cttctcactg ctacgacagt ctagcaccat ggcgtcttca + 5461 tcaactccac aacgccccat ggcctacccg tccatctctc gactgcgtgc atccatgggc + 5521 cgcgctaccg gctcctcgtt cgcactaccc aaacatctta acgacgtgtc tggagcatct + 5581 tcagcccatc tccctggcac gccttcagtg ccgtcgtcac cacgaaaagc tcgctcaccg + 5641 ctgccgttcg aatttaccgc caacggcctc tcggcagcgg ctgccgctgc ccattcatcc + 5701 aaggacgacg atgcaagtcg ttccgccatc gcagagtctt ccattacgca gcgatccaga + 5761 caaagttctt actcaactag tgttagtggc tcgatcttcg gccaaaacaa gcctcgtcgc + 5821 tctcgtgctt cgtttcccta tggcggtaca tccaacgggc cgttagtgcc gacagcacag + 5881 agcgccacat cgctcctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn + 5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca + 6001 tatagccgtg actttgtcaa gtggtcagca ttagcatttt cgtcaccaat gcaaagtcga + 6061 gtcccgacgc tgccatcctg tacccactgt gcttgctgtg gccggcggtc tcatcgctgt + 6121 cggtactcgg ggtggtagat cgatggtctg cgattttgcg cagcagccaa tagcttcgtg + 6181 tggacacgac ggaggaaatc atggcgatcc tgtcaccgct ctcgcctttt cctctgacca + 6241 ttcgttcctt ggtgtcggcc acgcctcggg caacattttt ctttacgatc tatcgagtcc + 6301 gggacgtgcc gctcgtcatg tacccccagt acccttgtca tctgtcaagg caggcaaaaa + 6361 ggaaggccat ctatccggat cagccatcgt gcacattggc ttcatcggcc tccgccacac + 6421 tgccatcgtc accgctgaca ttaaaggcct tgccttctac catgccctcg gcaaaatcct + 6481 tggcgtcagc tccaatgaca ccctccgtct acttggcaag tatccgcaat acgagcacga + 6541 agatgagctt ggccttggcg acaccaagaa acgcaacaca atctttcagc tcagtccgtt + 6601 gcctcttgga ccgcggccac attcggccga tgaaacaagc ttcatcgcca tgattacgcc + 6661 tacaaaaatg gtgctcgtcg gccttaagcc ttctcctcgc acgtggtacc gcaaggtcaa + 6721 ccgtgcacag ccgacagaga agcaggacat agctgcaccg gtgaatgagc agccagttca + 6781 agaaccgagc tgcctcgcct ggtatccagc ctcaacagag acggatggca agcgcaggcg + 6841 acagaccaat ccgatcctcg ctttctcgtt tggagccacc ctgcatcttg tgcgcttgaa + 6901 ggtcaagagg gtggatcagc caccggatcc aagaaaccct cgttctacta atggtgccgg + 6961 agccggatcc ggatccaatg tcgtgggcga agaggtcgat ccagtcgaac ttactggcga + 7021 gaatggcatc gaagagcctg atcacattgt cgcgctgcaa tggttcagcc ctgatctgct + 7081 tctgctcacc acaacttcag gccttgcact ctttgactgt cgtgctggca aagtgaccga + 7141 acgtatgagg ggtggtagtg catccgcgat attggccaag acagtcgagc aacgctatta + 7201 tgatgctgtt ctgctcggca agcactacga tccacataca gagacacagg acaatcacac + 7261 caactcaaac ggaacaaata gagtccgttc ttgggccctt aatcacagcg tacgcatttc + 7321 aaaggggcga tgcttctttt tgaccgagtc ggacctcgta gtaggtactc ttctctcctg + 7381 ggcagaccga ttgcttctct acgttgtcca aggcgacttt ttgtctgcca tcgagctggc + 7441 tactctcttc caccaaggca aattcttggg ttccgctgtc ggcctgccat ccgatcccaa + 7501 cgagcgcact ctcatcgtcg ctgacaagct tcgcgagctc atgtctgcat ctgcggagta + 7561 tgccttctcg ccggaccggc tcaccgatgc cacgcatgta acgccagata accggggcgt + 7621 tgatagaacc ttgctcttcg agggtctcgc tcgcgtctgt gcacgtgcct gtctctcact + 7681 tggcaacctc gactttctct ttgacacttt gtacgacaag tacgaggact atggtatcga + 7741 agccatcttt gttggccaga tggaggactt tatcgtgtcg ggtgagcttc gctctctacc + 7801 cattcccgtc gtccagagat tggtcgcctt ccgtagagac aggcaagagt atgctcaggc + 7861 agagcgtatc atctggcatg tcgatccaaa atcgctcgat ttggaccaag ctttgtcact + 7921 gtgtctagac aggaggctct acgacgcttt gatttatgtg ttcaatgctg ctctggagga + 7981 ttatgtttcg ccaattattg agctgttgca tccgttgagg cgcgctattg gtcatcggcg + 8041 aaggcatcag cagagcgtta cggcaaattc cattcaagca agtgacgatc catatgccct + 8101 catccgcgtc acggaggcgg cagatctgca tcagccagac ccaacctcag actgggagct + 8161 ggaggagaaa gatatcgagg atgcctaccg agtcttttcg tacctttccg tccttctgac + 8221 aggcaacaga taccccagtc aagagccttt tcaggaggac gaacaggcga gcaaggccaa + 8281 gagctccatc tattcttttg tcttttctgg cggctgcacg ctgtggcctc caggccctgg + 8341 cggaaaactg gtactctacg gactggacga cgacgcgaac cccagcaatc ttcgcgaaga + 8401 cgggaaagac caggttgcag agcacgaacc gatctatccg tatcttcgat tgctgctcga + 8461 attcgacgct gaagctttcc tcgatgcact cgacctcgcc ttcgaagacg gtttcctgga + 8521 cgacgaagac gtggtaggga agcgcgtcag caggcagctt attgttgatg ttttgcttga + 8581 gctgaacaat gcgtcgctcc ccgaaagacc cgacttagca ggcaacttgg tgtcacgtcg + 8641 ctcgcaagat gcgaaatcgc ccaagatgcc gttggccgca cgcatctttg cccacatttt + 8701 tgtcgcgcgc aatgcgccca agtatccgca atttatccgc ctttcacagc agaatgtcga + 8761 tctgcttttg cgctcgctcg cgtgcccctc tccaatgagt agggatgcaa aggaggatct + 8821 ggaggcgatc gagtcgacac acgaggaccg agaattagca gccgagtgtc ttctttcaca + 8881 ctacaagccg cgactcgacg acgactggct ggacacgttc gagaaagcct cattccatcg + 8941 gatcttgcga tctgtcttcc gccaggagaa gaaatgggat cggttgcttg ggatgtacct + 9001 tcgacagcat ttgcagacag gccagtctga ccctgttgat aggaacagcg gagctgaagc + 9061 caacgcccca ggtggtgatg ccttttccca ctttgaagag gtactgttca acgcggcgcg + 9121 caaaaatact ccggaaaggg acgtgcttca gactcgactg cacgagcaac tttccgatca + 9181 cattatcgac ctgcttgacc tcgatccgct caagacgact gggttgctgg acagattctt + 9241 tccgggcaag catgagtcgg tgctgcgtcg attggagcag ctggatgaaa agaggttatt + 9301 gctgtacttg cgttgctttt tcgagccgtt acatgtcagc aatgatagtg cactccacgt + 9361 gcctttcgca gaccgcgttg acaatgaagc gggtttcaac tggaccggca ttagggcacc + 9421 aggcacagcg acgtacattg tcgatgcagt tcgagccaat ccagagcatc tgccacgcaa + 9481 agagcaagat gcatatcttg atctcttgtg tcgatatgac ccggaaaccg tggttcgcaa + 9541 cctcgatgcc aagggtgccg actacttcga ccttgagcag cttgtgcagc tgtgtcgaag + 9601 taggaagacg gcgatcgatg gtagcagagt tgcagtcaac agcgatgctg ttctgtgggc + 9661 gctcgatcgc cttggacaat ccaacgaagc cttcgaagag ctggacacgg cgtgtgccag + 9721 gacagctgat tcgctggtct atgtggcctc agcggactcg ttcggtaccg atgcagcgga + 9781 gacagttgcg gatgaattgc taggggacgt aaggagatgt gtcaacgtcg ctgtgcggct + 9841 gtgtatcgag cgagccaatg catatggtgg agacgaggag actgacgagg aggacagtgg + 9901 tacacgaaag cgaggaaagc gagcagaaaa gcgattggat acggacgaag tgtggtatcg + 9961 ccttcttcga agtctcgtcc gactggttca tgatgttgct catctcggtg tcgccgacaa + 10021 ccatcagggt gaacgctaca acgggtctcg ccctaagaag ttactcgatg gcgttcgtga + 10081 tattgtccaa gacacactct cggacctcat ctcgtccacc gcggctgaag ccgtctcctt + 10141 ccccgcactc tttcgccgac ttaccggttc ggacaacggc aggacatctg cagccggcag + 10201 taaggcgatc aaagcagaat cgcaagctgg tgtagctgag tactacgccg agatccgatc + 10261 tgtactggat ggtatgctat ccgcctacca cttacgatcg gaggtgctag ccatcacgaa + 10321 taagcttttc gatcgcgata cgttccatca gtttcgccgc ttgcacatgc agcggaagca + 10381 cggctggcgc cctgcagggg gcacgagcac tcgatgcgcc gggtgtgacg tacttgtcat + 10441 cggaccacga cgaggtgttt ccaccttcta tcccgtcgca ttgtacgaaa atggcacgaa + 10501 catactcccc ggctcaccag aagcaggaaa agaagcggag accgaagatg tgcgaaggac + 10561 ggcattccaa ccctcgcgta gcacgtcttt cacgtctgtc gctgaaacac gtcgcagcag + 10621 cggtggtgtt cagctcggct cgatgctatc gcctccggca tcgctgttgc agccacatcg + 10681 gcgccgagcc aagtcgccgt acgacgagaa ggctgcgccg ctcgcttcac cgcgtatcga + 10741 caagggcaaa ggtgtcgtac ggtccgcttc atctgaattt tcacgcatca acggcgacca + 10801 tgcggtgctc ggtaagggca aagcgaatcg tagacacgca agcgaaggtg gggaaggaga + 10861 actacacgaa gtggatggct actttgccag cgagtttacg gatcgtcctt cgtggtctac + 10921 tcagcggccg actggacaaa gcagcgccgt catgacgagg tcgaacagtg tagcaagtag + 10981 tgctggtgtt tcgctacgca tcaacgatgc gcttgccgat gcacagcagc acttggaagc + 11041 aactgaggag gatgcagatg gaacgctgac acaagagcac tttggacagg ctgcgagcgc + 11101 ggattcagcg catttgaacg cagctacgtt gtcgtcggat gaaatgcaac gcgaaacgga + 11161 tgtggtcgag ggcgggatcg aggcggagag gatcgtggtt caaaagtcag ggcgcgtgta + 11221 ccaccaacgt tgctggcagc gtttgcatgc tgcggccaac gcggccacca tggtgagcaa + 11281 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa + 11341 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac + 11401 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac + 11461 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt + 11521 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga + 11581 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat + 11641 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta + 11701 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt + 11761 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca + 11821 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac + 11881 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt + 11941 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccggcg + 12001 cgccggccgc ccggcgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct + 12061 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata + 12121 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa + 12181 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg + 12241 cgcgcggtgt catctatgtt actagatccg atgataagct gtcaaacatg agaattgcgg + 12301 ccgcactcga gtggcggcag atgtgagtcg tgtgctacaa aacgtgaatc gacacgcgca + 12361 gggcggaacc aaaaaaaacc cccaccccgc tgtcaagttg acaaatcaac acatttgttc + 12421 caagttcaaa tcgggaaatc aaaaattagg gccagatcag cgatcaggaa tggtaatcgg + 12481 taacagaggt cgcaaaatcg tctagaaagt ggaagaagaa cgtggtaact accagcgagt + 12541 tctgcaaact tcaaaaaaaa aatctgggca cgatgaaagt tgagctaacg ctgacgctca + 12601 caaatggcgt ggctaaagga agcgagacaa tcggaaaatt gttctctcgg gcaccacaaa + 12661 gctgttgtta gtcgctgaag aacaattcca actgattccg ccgccttcct attgcgtcag + 12721 ccttgtacct aagctgccga gtaacgtcac tcaacctctc ttttcagact gctttgctcc + 12781 gcgaatactt ttcttctatg cgctcaagaa aatgacacag cacaccaagc tctgcaaact + 12841 ttcttcgcta atctgacgcg aaatgtgagc catttcttct cgcctgcaat ggcaatgcgt + 12901 ctgtgcggcg atgagaatca cgatgcggaa tgggtggctg gaagttcata gagatgctga + 12961 gttgttggag cgacatggta cataagcatg agtctgtcct gatttccacc ctcccgtctt + 13021 tcatcaactt tctcgtctga cccttccgtt gccagatccc ggggggcaat gagatatgaa + 13081 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt + 13141 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg + 13201 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta + 13261 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga + 13321 actcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga + 13381 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat + 13441 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg + 13501 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg + 13561 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat + 13621 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa + 13681 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt + 13741 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat + 13801 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct + 13861 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa + 13921 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg + 13981 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt + 14041 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata + 14101 gagtagatgc cgaccgggat ctgggcagca ccaagacacg gaacttcaac agtcgtgagt + 14161 aggtggtgat ctgcatgtcc aaatcgtgaa acgtgaatca cgaaccacga atcgtgaata + 14221 gccactttgc gcgaaaatca ctgaatgccg ccaaaccgac gcgacgcgag ttgcgtgaag + 14281 tattcgtgat tcacattcac gattgacgat tgtgagttgg tcgacgacat tcgctagcca + 14341 gaccaacact cacgactttt tgaagagcgt tgcaggctga gccaactttc tccctcactc + 14401 tttgctttct tctccatcac gctcattcct tttaagtgtt catccggtat cattagcact + 14461 atatcatagt gaaactcgca acaacccggt cttcgtcgcg aaagcaaccc tccatcgctg + 14521 ctgcctcttg cagtgaagac cgccaccatg cagtctacgc agtcgaagac cctacacgtc + 14581 cagttcgtcc attctggtcc tacacattgt atccacgcac ccaagtctgc caacgtggag + 14641 taagtgaacc tttctttgtc ctgtcgaggc tcaacgtaga tcacaggctg atcatccaca + 14701 ctcttctacc ttttgacatt acgcagtcga ctttttacag gaggagatga ctacctcgtc + 14761 cgaatcttac ccaccctgcc tctctccgac gtagagccgc agcttatcga agacgccact + 14821 gaagcggtga cctcgctcga tgctgacggc cgcttcctcg tcaccgcttc cgaagatggt + 14881 gtcaacgcct ctaccgacac cacccaatcg acgcggaagg caacccagca tcaccggctg + 14941 tgcttcaatc gctgctgcgc cgcgaagctg tgcggccgca ttaataggcc tgagtggcc +// +LOCUS pUL404 14063 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of SfiI casset into Fragment (Vps8) +ACCESSION pUL404 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 14063) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: Fragment (Vps8) + Fragment ends: SfiI + Fragment size: 11267 + Insert molecule: SfiI casset + Fragment ends: SfiI + Fragment size: 3733 +FEATURES Location/Qualifiers + misc_signal 1..24 + /gene="(null)" + /SECDrawAs="Label" + misc_feature 1..1016 + /gene="DF" + /SECDrawAs="Region" + CDS complement (161..856) + /gene="UMAG_11502" + /SECDrawAs="Gene" + misc_signal 991..1016 + /gene="(null)" + /SECDrawAs="Label" + misc_signal 1014..1016 + /gene="SspI 1st half" + /SECDrawAs="Label" + CDS complement (1022..1089) + /gene="lacZ'" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 2390..2390 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (2246..3106) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" + misc_signal 3697..3736 + /gene="(null)" + /SECDrawAs="Label" + CDS complement (3452..3712) + /gene="lacZ" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 3723..3725 + /gene="SspI 2nd half" + /SECDrawAs="Label" + misc_feature 3723..4737 + /gene="UF" + /SECDrawAs="Region" + CDS complement (3723..3944) + /gene="UMAG_02910'" + /SECDrawAs="Gene" + misc_signal 4736..4736 + /gene="g to C NcoI site-muttation" + /SECDrawAs="Label" + CDS 4738..4959 + /gene="Exon1-Vps8" + /SECDrawAs="Gene" + CDS 4780..4830 + /gene="HA-tag" + /SECDrawAs="Gene" + misc_signal 4960..5098 + /gene="(null)" + /SECDrawAs="Label" + misc_feature 4960..5098 + /gene="intron" + /SECDrawAs="Region" + CDS 5099..10315 + /gene="Exon2-Vps8" + /SECDrawAs="Gene" + CDS 5099..11053 + /gene="Exon2-egfp" + /SECDrawAs="Gene" + CDS 10334..11053 + /gene="egfp" + /SECDrawAs="Gene" + misc_feature 11054..11356 + /gene="nosT" + /SECDrawAs="Region" + misc_feature complement (11363..11911) + /gene="hsp70T" + /product="hsp70 terminator" + /SECDrawAs="Region" + CDS complement (11912..13240) + /gene="hygR (hph)" + /SECDrawAs="Gene" + misc_feature complement (13241..14036) + /gene="P-hsp70" + /product="hsp70 promoter" + /SECDrawAs="Region" +ORIGIN + 1 ttcttgcaca tagtgattct ttgctcagtg tgattcttgc atgtgcgcaa cccattcatg + 61 tatccagatc tctgtacgct gaagatttgt gctgatgcga acgtcatttt gctttgcgtc + 121 aagtatgatt gtgagatggt cgcgtgatag agccgaagga tcaagaggat ttggcgttga + 181 cctcagcagg taaatcggca gcgacagccg cggcggtagc atcggctgtc gagggtgtgg + 241 tctgcgtacc gaatccacct gtcgacccaa accctccagc acccctaaga gtctcgctca + 301 agctttcaac ttcttgaacg tcgggtgtcg agatcttctc aaggatcaac tgagcgatgc + 361 ggtcaccttg gttaactgtt tcgttggcag accgcgttga tgtcagctcg gatcggttgc + 421 atgttgcgcg caaacaagaa tcgcacttac tgatgaagtc ttgttcgccc aagttgaaca + 481 gcaagacgcc gagcaagcca cggtagtctt ggtcgatcac accggctccc gtgtcaatgc + 541 cgtgtttgct cgcaagccct gaacgcggtg caacacggcc gtagtgtccg tcgggaatcg + 601 cgacgcagat accggtctga accaccttgc gaccgccttg tggaagcacc actttttcgg + 661 cagagtacag atcgtagccg gccgcaagcg ccgacccgcg cgtaggcagc ttggcgagcg + 721 gatgcgaagg gtgacgctgc accagcagcc gcactgtaga gttgacgctc atcgtaccgg + 781 cctcttcgag cttggcgagt ttcgtaggtc gagcttcgtc gcttgtacga gcgtcagagc + 841 agatgttcga cgtcatgaga gaaagcgaac gagcacgaac tggtatcgtc aggatcaacg + 901 gggcaaaggt gaaccatgaa ccgtgaacgc gttcgccacg agtgaatgtg ttattctctc + 961 gcccagtcac aatcaccaat cacgaattca cgattctcgg cgcgtgtcgc gtaaatattg + 1021 tctagatatc ggatcccagg cccgtcgact gcagaggcct gcatgcaagc ttggcgtaat + 1081 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac + 1141 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa + 1201 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat + 1261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc + 1321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg + 1381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag + 1441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc + 1501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag + 1561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga + 1621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc + 1681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg + 1741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt + 1801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca + 1861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca + 1921 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag + 1981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca + 2041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg + 2101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa + 2161 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta + 2221 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag + 2281 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga + 2341 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagat ccacgctcac + 2401 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc + 2461 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta + 2521 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac + 2581 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat + 2641 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa + 2701 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg + 2761 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag + 2821 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc + 2881 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct + 2941 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat + 3001 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg + 3061 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc + 3121 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta + 3181 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg + 3241 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct + 3301 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga + 3361 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag + 3421 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga + 3481 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca + 3541 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt + 3601 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc + 3661 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcacctgca + 3721 atattgggca agcaaggagt gaagtcatcc ggatgaccgt caagagcaaa gtggagcgtt + 3781 tggtgaatgg catgcgcgag ctctccatcg gccgatggtt tcagcgatct tgatacgtcg + 3841 tgcgtgtgaa tgtacaacga gcgaaagccg gacttgttgt ccgttagcgg acaagcagat + 3901 gctgttctag ctgttgttgc cgttgttgcc gttgctgagg tcatattgtg tgtggtggta + 3961 tggcaagctg tgtcaaggtg tcgcagagca aagccgagaa cagcacgcct aattctcatc + 4021 ttttgtcaaa ttacgactcg agccaagcac gaagcaagcg agtcagtatc acgattagtg + 4081 aatcagtgat tcgtgattcg aaagcggcaa tacaagccat gtggctccgt cattcagccc + 4141 ttccgaggag tgccgtcgct cggttccgtc acgcgttaaa cccacatgtg cccgtgccca + 4201 gtagttacgt attgttagca gcgtgaagca taacatgtgt gtataatatc cgtgatccgt + 4261 gatccacgat acacaatcac gaatcgtgaa tcactcgtga ctgaatatcg tgaatatcgt + 4321 gaatcgccaa tcgtgaatga gagtgtaact cacgactcca cgactcgtga ctgtcatgat + 4381 atttgtgatt catacaatca cgaagaacgc acggcctttc gtatttctct ctgtgttgcc + 4441 gcttttcacc gattttcgtc ctcgcaacgc catcgattgc aagtttgcga tagccgacat + 4501 cagtcgtcga ccatttcttg cctcccgcaa agtagtggcc gaagcagttg gccatccgat + 4561 tgcagcgcgg atctgcttta ccaacgactc tgcaccacta ggcacagcgg tccagcaacc + 4621 ccccaccatg tccaattgca tacgctctcg ctctcgcacc atcagatagg tgtcgtcaac + 4681 agcaccgcca ctatcacccc cgttcatcat catcattccc gctgcagcgt cttcgccatg + 4741 gaacacaaag actctgctga cttgaatcct tggggtgctt acccttacga cgtgcccgac + 4801 tacgccctgg aagtactgtt ccaggggccc tcttactcaa ctagtgttag tggctcgatc + 4861 ttcggccaaa acaagcctcg tcgctctcgt gcttcgtttc cctatggcgg tacatccaac + 4921 gggccgttag tgccgacagc acagagcgcc acatcgctcc tcnnnnnnnn nnnnnnnnnn + 4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn + 5041 nnnnnnnnnn nnnnnnnnnn nncatatagc cgtgactttg tcaagtggtc agcattagca + 5101 ttttcgtcac caatgcaaag tcgagtcccg acgctgccat cctgtaccca ctgtgcttgc + 5161 tgtggccggc ggtctcatcg ctgtcggtac tcggggtggt agatcgatgg tctgcgattt + 5221 tgcgcagcag ccaatagctt cgtgtggaca cgacggagga aatcatggcg atcctgtcac + 5281 cgctctcgcc ttttcctctg accattcgtt ccttggtgtc ggccacgcct cgggcaacat + 5341 ttttctttac gatctatcga gtccgggacg tgccgctcgt catgtacccc cagtaccctt + 5401 gtcatctgtc aaggcaggca aaaaggaagg ccatctatcc ggatcagcca tcgtgcacat + 5461 tggcttcatc ggcctccgcc acactgccat cgtcaccgct gacattaaag gccttgcctt + 5521 ctaccatgcc ctcggcaaaa tccttggcgt cagctccaat gacaccctcc gtctacttgg + 5581 caagtatccg caatacgagc acgaagatga gcttggcctt ggcgacacca agaaacgcaa + 5641 cacaatcttt cagctcagtc cgttgcctct tggaccgcgg ccacattcgg ccgatgaaac + 5701 aagcttcatc gccatgatta cgcctacaaa aatggtgctc gtcggcctta agccttctcc + 5761 tcgcacgtgg taccgcaagg tcaaccgtgc acagccgaca gagaagcagg acatagctgc + 5821 accggtgaat gagcagccag ttcaagaacc gagctgcctc gcctggtatc cagcctcaac + 5881 agagacggat ggcaagcgca ggcgacagac caatccgatc ctcgctttct cgtttggagc + 5941 caccctgcat cttgtgcgct tgaaggtcaa gagggtggat cagccaccgg atccaagaaa + 6001 ccctcgttct actaatggtg ccggagccgg atccggatcc aatgtcgtgg gcgaagaggt + 6061 cgatccagtc gaacttactg gcgagaatgg catcgaagag cctgatcaca ttgtcgcgct + 6121 gcaatggttc agccctgatc tgcttctgct caccacaact tcaggccttg cactctttga + 6181 ctgtcgtgct ggcaaagtga ccgaacgtat gaggggtggt agtgcatccg cgatattggc + 6241 caagacagtc gagcaacgct attatgatgc tgttctgctc ggcaagcact acgatccaca + 6301 tacagagaca caggacaatc acaccaactc aaacggaaca aatagagtcc gttcttgggc + 6361 ccttaatcac agcgtacgca tttcaaaggg gcgatgcttc tttttgaccg agtcggacct + 6421 cgtagtaggt actcttctct cctgggcaga ccgattgctt ctctacgttg tccaaggcga + 6481 ctttttgtct gccatcgagc tggctactct cttccaccaa ggcaaattct tgggttccgc + 6541 tgtcggcctg ccatccgatc ccaacgagcg cactctcatc gtcgctgaca agcttcgcga + 6601 gctcatgtct gcatctgcgg agtatgcctt ctcgccggac cggctcaccg atgccacgca + 6661 tgtaacgcca gataaccggg gcgttgatag aaccttgctc ttcgagggtc tcgctcgcgt + 6721 ctgtgcacgt gcctgtctct cacttggcaa cctcgacttt ctctttgaca ctttgtacga + 6781 caagtacgag gactatggta tcgaagccat ctttgttggc cagatggagg actttatcgt + 6841 gtcgggtgag cttcgctctc tacccattcc cgtcgtccag agattggtcg ccttccgtag + 6901 agacaggcaa gagtatgctc aggcagagcg tatcatctgg catgtcgatc caaaatcgct + 6961 cgatttggac caagctttgt cactgtgtct agacaggagg ctctacgacg ctttgattta + 7021 tgtgttcaat gctgctctgg aggattatgt ttcgccaatt attgagctgt tgcatccgtt + 7081 gaggcgcgct attggtcatc ggcgaaggca tcagcagagc gttacggcaa attccattca + 7141 agcaagtgac gatccatatg ccctcatccg cgtcacggag gcggcagatc tgcatcagcc + 7201 agacccaacc tcagactggg agctggagga gaaagatatc gaggatgcct accgagtctt + 7261 ttcgtacctt tccgtccttc tgacaggcaa cagatacccc agtcaagagc cttttcagga + 7321 ggacgaacag gcgagcaagg ccaagagctc catctattct tttgtctttt ctggcggctg + 7381 cacgctgtgg cctccaggcc ctggcggaaa actggtactc tacggactgg acgacgacgc + 7441 gaaccccagc aatcttcgcg aagacgggaa agaccaggtt gcagagcacg aaccgatcta + 7501 tccgtatctt cgattgctgc tcgaattcga cgctgaagct ttcctcgatg cactcgacct + 7561 cgccttcgaa gacggtttcc tggacgacga agacgtggta gggaagcgcg tcagcaggca + 7621 gcttattgtt gatgttttgc ttgagctgaa caatgcgtcg ctccccgaaa gacccgactt + 7681 agcaggcaac ttggtgtcac gtcgctcgca agatgcgaaa tcgcccaaga tgccgttggc + 7741 cgcacgcatc tttgcccaca tttttgtcgc gcgcaatgcg cccaagtatc cgcaatttat + 7801 ccgcctttca cagcagaatg tcgatctgct tttgcgctcg ctcgcgtgcc cctctccaat + 7861 gagtagggat gcaaaggagg atctggaggc gatcgagtcg acacacgagg accgagaatt + 7921 agcagccgag tgtcttcttt cacactacaa gccgcgactc gacgacgact ggctggacac + 7981 gttcgagaaa gcctcattcc atcggatctt gcgatctgtc ttccgccagg agaagaaatg + 8041 ggatcggttg cttgggatgt accttcgaca gcatttgcag acaggccagt ctgaccctgt + 8101 tgataggaac agcggagctg aagccaacgc cccaggtggt gatgcctttt cccactttga + 8161 agaggtactg ttcaacgcgg cgcgcaaaaa tactccggaa agggacgtgc ttcagactcg + 8221 actgcacgag caactttccg atcacattat cgacctgctt gacctcgatc cgctcaagac + 8281 gactgggttg ctggacagat tctttccggg caagcatgag tcggtgctgc gtcgattgga + 8341 gcagctggat gaaaagaggt tattgctgta cttgcgttgc tttttcgagc cgttacatgt + 8401 cagcaatgat agtgcactcc acgtgccttt cgcagaccgc gttgacaatg aagcgggttt + 8461 caactggacc ggcattaggg caccaggcac agcgacgtac attgtcgatg cagttcgagc + 8521 caatccagag catctgccac gcaaagagca agatgcatat cttgatctct tgtgtcgata + 8581 tgacccggaa accgtggttc gcaacctcga tgccaagggt gccgactact tcgaccttga + 8641 gcagcttgtg cagctgtgtc gaagtaggaa gacggcgatc gatggtagca gagttgcagt + 8701 caacagcgat gctgttctgt gggcgctcga tcgccttgga caatccaacg aagccttcga + 8761 agagctggac acggcgtgtg ccaggacagc tgattcgctg gtctatgtgg cctcagcgga + 8821 ctcgttcggt accgatgcag cggagacagt tgcggatgaa ttgctagggg acgtaaggag + 8881 atgtgtcaac gtcgctgtgc ggctgtgtat cgagcgagcc aatgcatatg gtggagacga + 8941 ggagactgac gaggaggaca gtggtacacg aaagcgagga aagcgagcag aaaagcgatt + 9001 ggatacggac gaagtgtggt atcgccttct tcgaagtctc gtccgactgg ttcatgatgt + 9061 tgctcatctc ggtgtcgccg acaaccatca gggtgaacgc tacaacgggt ctcgccctaa + 9121 gaagttactc gatggcgttc gtgatattgt ccaagacaca ctctcggacc tcatctcgtc + 9181 caccgcggct gaagccgtct ccttccccgc actctttcgc cgacttaccg gttcggacaa + 9241 cggcaggaca tctgcagccg gcagtaaggc gatcaaagca gaatcgcaag ctggtgtagc + 9301 tgagtactac gccgagatcc gatctgtact ggatggtatg ctatccgcct accacttacg + 9361 atcggaggtg ctagccatca cgaataagct tttcgatcgc gatacgttcc atcagtttcg + 9421 ccgcttgcac atgcagcgga agcacggctg gcgccctgca gggggcacga gcactcgatg + 9481 cgccgggtgt gacgtacttg tcatcggacc acgacgaggt gtttccacct tctatcccgt + 9541 cgcattgtac gaaaatggca cgaacatact ccccggctca ccagaagcag gaaaagaagc + 9601 ggagaccgaa gatgtgcgaa ggacggcatt ccaaccctcg cgtagcacgt ctttcacgtc + 9661 tgtcgctgaa acacgtcgca gcagcggtgg tgttcagctc ggctcgatgc tatcgcctcc + 9721 ggcatcgctg ttgcagccac atcggcgccg agccaagtcg ccgtacgacg agaaggctgc + 9781 gccgctcgct tcaccgcgta tcgacaaggg caaaggtgtc gtacggtccg cttcatctga + 9841 attttcacgc atcaacggcg accatgcggt gctcggtaag ggcaaagcga atcgtagaca + 9901 cgcaagcgaa ggtggggaag gagaactaca cgaagtggat ggctactttg ccagcgagtt + 9961 tacggatcgt ccttcgtggt ctactcagcg gccgactgga caaagcagcg ccgtcatgac + 10021 gaggtcgaac agtgtagcaa gtagtgctgg tgtttcgcta cgcatcaacg atgcgcttgc + 10081 cgatgcacag cagcacttgg aagcaactga ggaggatgca gatggaacgc tgacacaaga + 10141 gcactttgga caggctgcga gcgcggattc agcgcatttg aacgcagcta cgttgtcgtc + 10201 ggatgaaatg caacgcgaaa cggatgtggt cgagggcggg atcgaggcgg agaggatcgt + 10261 ggttcaaaag tcagggcgcg tgtaccacca acgttgctgg cagcgtttgc atgctgcggc + 10321 caacgcggcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct + 10381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg + 10441 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt + 10501 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc + 10561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga + 10621 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga + 10681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa + 10741 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga + 10801 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag + 10861 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct + 10921 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg + 10981 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga + 11041 gctgtacaag taaagcggcc ggcgcgccgg ccgcccggcg atcgttcaaa catttggcaa + 11101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg + 11161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg + 11221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag + 11281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tccgatgata + 11341 agctgtcaaa catgagaatt gcggccgcac tcgagtggcg gcagatgtga gtcgtgtgct + 11401 acaaaacgtg aatcgacacg cgcagggcgg aaccaaaaaa aacccccacc ccgctgtcaa + 11461 gttgacaaat caacacattt gttccaagtt caaatcggga aatcaaaaat tagggccaga + 11521 tcagcgatca ggaatggtaa tcggtaacag aggtcgcaaa atcgtctaga aagtggaaga + 11581 agaacgtggt aactaccagc gagttctgca aacttcaaaa aaaaaatctg ggcacgatga + 11641 aagttgagct aacgctgacg ctcacaaatg gcgtggctaa aggaagcgag acaatcggaa + 11701 aattgttctc tcgggcacca caaagctgtt gttagtcgct gaagaacaat tccaactgat + 11761 tccgccgcct tcctattgcg tcagccttgt acctaagctg ccgagtaacg tcactcaacc + 11821 tctcttttca gactgctttg ctccgcgaat acttttcttc tatgcgctca agaaaatgac + 11881 acagcacacc aagctctgca aactttcttc gctaatctga cgcgaaatgt gagccatttc + 11941 ttctcgcctg caatggcaat gcgtctgtgc ggcgatgaga atcacgatgc ggaatgggtg + 12001 gctggaagtt catagagatg ctgagttgtt ggagcgacat ggtacataag catgagtctg + 12061 tcctgatttc caccctcccg tctttcatca actttctcgt ctgacccttc cgttgccaga + 12121 tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt + 12181 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc + 12241 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc + 12301 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat + 12361 tccggaagtg cttgacattg gggaactcag cgagagcctg acctattgca tctcccgccg + 12421 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc + 12481 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg + 12541 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat + 12601 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt + 12661 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct + 12721 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt + 12781 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt + 12841 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc + 12901 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact + 12961 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga + 13021 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc + 13081 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag + 13141 cactcgtccg agggcaaagg aatagagtag atgccgaccg ggatctgggc agcaccaaga + 13201 cacggaactt caacagtcgt gagtaggtgg tgatctgcat gtccaaatcg tgaaacgtga + 13261 atcacgaacc acgaatcgtg aatagccact ttgcgcgaaa atcactgaat gccgccaaac + 13321 cgacgcgacg cgagttgcgt gaagtattcg tgattcacat tcacgattga cgattgtgag + 13381 ttggtcgacg acattcgcta gccagaccaa cactcacgac tttttgaaga gcgttgcagg + 13441 ctgagccaac tttctccctc actctttgct ttcttctcca tcacgctcat tccttttaag + 13501 tgttcatccg gtatcattag cactatatca tagtgaaact cgcaacaacc cggtcttcgt + 13561 cgcgaaagca accctccatc gctgctgcct cttgcagtga agaccgccac catgcagtct + 13621 acgcagtcga agaccctaca cgtccagttc gtccattctg gtcctacaca ttgtatccac + 13681 gcacccaagt ctgccaacgt ggagtaagtg aacctttctt tgtcctgtcg aggctcaacg + 13741 tagatcacag gctgatcatc cacactcttc taccttttga cattacgcag tcgacttttt + 13801 acaggaggag atgactacct cgtccgaatc ttacccaccc tgcctctctc cgacgtagag + 13861 ccgcagctta tcgaagacgc cactgaagcg gtgacctcgc tcgatgctga cggccgcttc + 13921 ctcgtcaccg cttccgaaga tggtgtcaac gcctctaccg acaccaccca atcgacgcgg + 13981 aaggcaaccc agcatcaccg gctgtgcttc aatcgctgct gcgccgcgaa gctgtgcggc + 14041 cgcattaata ggcctgagtg gcc +// +LOCUS pUMa1755 9101 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of G418 into 1391-BB +ACCESSION pUMa1755 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 9101) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: 1391-BB + Fragment ends: SfiI + Fragment size: 7028 + Insert molecule: G418 + Fragment ends: SfiI + Fragment size: 2073 +FEATURES Location/Qualifiers + misc_feature 344..1408 + /gene="UF" + /SECDrawAs="Region" + misc_signal 344..1408 + /gene="UF" + /SECDrawAs="Label" + misc_feature 1451..3467 + /gene="GentR" + /SECDrawAs="Region" + misc_feature 1451..2361 + /gene="Potef" + /SECDrawAs="Region" + misc_feature 1485..1776 + /gene="operator 1" + /SECDrawAs="Region" + misc_feature 1792..2083 + /gene="operator 2" + /SECDrawAs="Region" + CDS 2176..2341 + /gene="tef" + /SECDrawAs="Gene" + CDS 2365..3159 + /gene="neo " + /SECDrawAs="Gene" + misc_feature 3176..3467 + /gene="Tcyc1" + /SECDrawAs="Region" + misc_feature 3493..5457 + /gene="DF" + /SECDrawAs="Region" + CDS 3970..4734 + /gene="cytochrome b561" + /SECDrawAs="Gene" + misc_signal 4330..4332 + /gene="Sequenz checken!" + /product="Punktmutation führt zu Valin anstatt + Asparagin" + /SECDrawAs="Label" + CDS 6489..7283 + /gene="kanR" + /product="Kanamycin resistance ORF" + /SECDrawAs="Gene" + CDS 7301..8161 + /gene="ampR" + /product="Ampicillin resistance ORF" + /SECDrawAs="Gene" +ORIGIN + 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc + 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc + 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa + 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat + 241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca + 301 ctagtaacgg ccgccagtgt gctggaattc gcccttgaca tttaaatttc attcacgatt + 361 caccctttcc aggccgaggc cctatgccgc aagtcacgtg gaattagttt cggggtcatc + 421 atgggacaaa catgcaaccg gcgtgaggct tgcggtttgg ggacattcgt gatttacgat + 481 gcacgattca cgattcacga ttcacgattc acgattcgtg atatctgatt ggagcaggtg + 541 cagtccaagc gatctaacaa ggagtttatg cctcacccct tacctctcac gcctcgccgc + 601 acgcatccac agtccacgat cgtgcatagc agatttgtag agtcgtgtgt tgaggtaaag + 661 acgttcacag tttcatgatt cacaaagcta acacgaatcg tgaatcacat taacatcgtt + 721 aagtagcaga taatcgtaat tgtgaatctg tgatttggtt taagtacgtg cgaaaacaca + 781 taagttacac actatcaaca tcacaaacac ggcctttgtg acacgcaatg gctaccacga + 841 cggcaaaatt cacagatttg atatctgtag cctattgatg tggacagaga ttgacctcga + 901 tcttgatctt gacctggtca acttttgtgg tcctcttact tgtgcttatc ttgaaccttc + 961 atgagtgtgg caaggtggca gaaggagggt gacatgccct aggtgcgctg aactctccac + 1021 cgcggccaca cgtgacacca cgcagtgcgt gtgttacttt cgcttccttt gagatctttc + 1081 ttggaagaac aagctcaagt tgaggttcac agacccaaaa accaaaaaaa aaaaaaaatc + 1141 gtgaatgaaa ggcggcgtgt gcatgcgtgt gtattgcatg acgatcattt caacgctcgt + 1201 cttgaatcgt ttgagtacga agcggcatgg cagctcgcac tccgaaagag actgtgcaac + 1261 tcactagagc ccgtcttcgg aagatccgtc tgcgactctg ctgcccacct gtagtttagc + 1321 gtcgtgatcc tcgacattgt ctcacgacca agacgacgac aacaacaaca acaacgacaa + 1381 cgtttcggct caagtcggaa ccttgccggc ctagatggcc accatggcgt gacaattgcg + 1441 gccgccgcga agcttgccgg cagatccgat gtacccgtac cgagctcgac tttcactttt + 1501 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga + 1561 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc + 1621 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga + 1681 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg + 1741 actttcactt ttctctatca ctgataggga gtggtaaact cgagtaccga gctcgacttt + 1801 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg + 1861 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc + 1921 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta + 1981 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt + 2041 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ggggatcaat + 2101 tcgaccaatg aggcgcgaga cgaggggacg ctggaagttg aggcgcaaga aaatttttct + 2161 ctggttctgc gcggcagaga cgaccagatt cgcccgcttt cttctgcgtt gggtgcctct + 2221 tttgggtgcc agactttgtg tgtgcgccag cgagacgttc caataaaggg cgctgtctcg + 2281 gcactatctt tctttctttc ctcatacatc gtatcatacc atacacagac aacatcatcc + 2341 acgggatcgc acgcgtagga tcatatgatt gaacaagatg gattgcacgc aggttctccg + 2401 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct + 2461 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac + 2521 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg + 2581 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg + 2641 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa + 2701 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca + 2761 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt + 2821 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc + 2881 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc + 2941 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg + 3001 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt + 3061 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag + 3121 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa + 3181 tcgtcccctt ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc + 3241 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc + 3301 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc + 3361 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga + 3421 aggttttggg acgctcgaag gctttaattt gcaagctgta ggagtgcggc cgcattaata + 3481 ggcctgagtg gcctccgagt gacaaccaga catatgttcc actgctaccg ttgtaggatt + 3541 gtaccaagga tgtggagctg aggtaaagtg cggctataca cacgcttaat gtgcggtgca + 3601 gcaagtcgtg attgggctac acacgtggtc gcggcggatg agcaagctcc gaatcacgaa + 3661 tcagaatgtc gcaatttgtg cgcgcgaatc acgattgcgg ttttcacgtt ttttgacagg + 3721 cggccaagca gccacgagat tcacgattat ctgatttggt cagcatagac ggcatggtat + 3781 gtctttcaac ggacaggttt cgagtgtagc atttcagcgc ttgttcgagt ttacgtcgat + 3841 caacacgcac gctctccact gttatcgatg gtccaaacta gttctgaggt tctctgagct + 3901 gactggtcga catcgtagcg gggttccgaa ggaaagactt ggtctcacgg cacaaacaca + 3961 agcgaagcca tgttgcaagg tcgcgcgagc gagcagggat ccaccacgca accactgctg + 4021 accaccacaa acgacgacta ccaccatcac gagacagacg ccttgatcac gccgctcgac + 4081 atggttcggc atgaaaacgt ctcgcgccat gcggtgggcg tgcaagtttc cgcgttgctc + 4141 tttgtcgtcg ttgtatggag cattgttttc tccactatgt cgatcctttc gctgccactc + 4201 ttcggctatc atccattgat ccagtctttc accatactgc ttctgctgca ggcgattgta + 4261 gtactgcagc ggacgacgcc atcacaacca gcggcgaaaa aatcggcgtt cacagcacac + 4321 cagtggatca acctcattct cgtactccca ctcttcaccg cgggcgcaag catcatgtgg + 4381 tacctgcacg atcaaccagc ctcggcacac ttcatatcgt accatgggat tctcggtacc + 4441 gccgtcgtaa tcgcagcgtg ggtacaggcc gccttcggtg ccgcaagcgt ctggtggaag + 4501 ggaaagctag tgggtggaga gaacaaaggt aaggcgcttt ggaagtggca tagattgtct + 4561 ggctatgtac tcattgcgct gtttctcttg acggctgtgt tgggtgtggt cgagactacg + 4621 tgggggaagc ggaatgcccg gccgatccag aagggcgtga cggtggtgac gttaagtttg + 4681 gcggcatttg cgatgctcat ccgcgtgcaa aagagcaaac tgccaaagct gtagtcttcc + 4741 aaccgcgctt aggtgtaatt ctaccatatt cttggaccgt gtctatgctt gccgtgcaat + 4801 aaactctgca attgtcaact atgaccaaga tcatacctgc tgcaaacgtt gtccggcttt + 4861 atcaagatgc gtctgaatcg actcagcagc tcgtcaagct cacgactaat ctgcttcgtt + 4921 tcaaagcatg aaggcttgaa caagaatgca ggaccatgtc ggcctcctca actctcccca + 4981 accttgccac tatcccgcaa aaggctggtc aaagaccgag agccagtgcc tgacgtcaaa + 5041 gaaatgacta caacaacagc aagcttccac agaagcatgc atggacgctg acaagcctcc + 5101 gctgggtcaa caagtgccac aaagcgaaag acgggggtta ttaaggtgtg aattgaaggg + 5161 cgcgtgcagt ttaggcatac atattcaatt gaaaaaaggg ggcccgacaa gcatatgttc + 5221 gcgaatagat gaggtaggga tggagggtat caatacgcca tagtataaag agaatcctca + 5281 ctgcctggca ccccacaggt gagccaagtc aaagagggga atgttaaagg gaaagtcggt + 5341 ggcggcgaga cacagatgaa gcagaatgta gaagccaccc atgcaagcag tgaaaccaca + 5401 agcaccggcg gcggtgatca ggttggcacc ggctgcgagg gagctttcgc cgaatttaaa + 5461 tgtcaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag + 5521 agggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg + 5581 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt + 5641 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag + 5701 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt + 5761 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc + 5821 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct + 5881 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat + 5941 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc + 6001 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc + 6061 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg + 6121 atttaacaaa aatttaacgc gaattttaac aaaattcagg gcgcaagggc tgctaaagga + 6181 agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct + 6241 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg + 6301 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc + 6361 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct + 6421 tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat + 6481 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga + 6541 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc + 6601 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga + 6661 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg + 6721 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc + 6781 cggggcagga tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg + 6841 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga + 6901 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc + 6961 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca + 7021 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg + 7081 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct + 7141 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg + 7201 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc + 7261 gccttcttga cgagttcttc tgaattgaaa aaggaagagt atgagtattc aacatttccg + 7321 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac + 7381 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact + 7441 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat + 7501 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga + 7561 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac + 7621 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat + 7681 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac + 7741 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct + 7801 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac + 7861 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga + 7921 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg + 7981 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact + 8041 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac + 8101 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta + 8161 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt + 8221 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga + 8281 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc + 8341 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt + 8401 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc + 8461 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc + 8521 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg + 8581 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg + 8641 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga + 8701 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc + 8761 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg + 8821 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg + 8881 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt + 8941 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc + 9001 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg + 9061 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g +// +LOCUS pUMa1915 8974 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of 1057 SfiI into 1574 SfiI +ACCESSION pUMa1915 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 8974) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: 1574 SfiI + Fragment ends: SfiI + Fragment size: 6901 + Insert molecule: 1057 SfiI + Fragment ends: SfiI + Fragment size: 2073 +FEATURES Location/Qualifiers + misc_feature 337..1391 + /gene="upa1_UF" + /product="um12183" + /SECDrawAs="Region" + misc_feature 1427..2337 + /gene="Potef" + /SECDrawAs="Region" + misc_feature 1427..3443 + /gene="GentR" + /SECDrawAs="Region" + misc_feature 1461..1752 + /gene="operator 1" + /SECDrawAs="Region" + misc_feature 1768..2059 + /gene="operator 2" + /SECDrawAs="Region" + CDS 2152..2317 + /gene="tef" + /SECDrawAs="Gene" + CDS 2341..3135 + /gene="neo " + /SECDrawAs="Gene" + misc_feature 3152..3443 + /gene="Tcyc1" + /SECDrawAs="Region" + misc_feature 3465..5338 + /gene="upa1_DF" + /product="um12183" + /SECDrawAs="Region" + CDS 4058..5179 + /gene="um12182" + /product="conserved hypothetical protein " + /SECDrawAs="Gene" + CDS 6362..7156 + /gene="kanR" + /product="Kanamycin resistance ORF" + /SECDrawAs="Gene" + CDS 7174..8034 + /gene="ampR" + /product="Ampicillin resistance ORF" + /SECDrawAs="Gene" +ORIGIN + 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc + 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc + 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa + 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat + 241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca + 301 ctagtaacgg ccgccagtgt gctggaattc gcccttaata tttgcacaaa tgaacggctc + 361 cttgtcttgg ctctctgcct gtgtttgccc ctcgcagatg cctgtgacac gacgatcaat + 421 cacaaacacg gtcttctcaa tcacgaatct cgttttcatt cacgattcgt aatggctttt + 481 gctcttgctc tggaactcac actgttccat tgtattgttc gtgttgcctc gaaacactcg + 541 actgagcgaa tgcgcagagg ggccaagtgc aggcacagca ctcgagcgac cacaacagcg + 601 gaggggcgtt aggggagcat accgtcggat ctgccgaacc agccacagtc acgagtgtgc + 661 acagcgtgtg aacgacagcg tgtgagagcg tgtgagagcg tgcgcgattg tgtgcgagcg + 721 tgtgtgttca gttcagtatg tcggtgtctt catttctgtg actgatctag tgctgcatga + 781 acgaggctcg gactagctgc cgaagtgtgg gcgcgtcgtg catgagctgc gtgccgcgtg + 841 cgtgcactgc attgatcaaa tcttgtgttg gtctcgctgt cggcgcttgt cgtgtgtggc + 901 atgcgccgaa agcatgtcgg ccactttccg tagcccaact cggcactcgc agactcatga + 961 ctgtctcttt tccctcatgc ctttcgtgta tggccactcc tctttccatg atccaagatt + 1021 aatccgggaa ccctgagcca acaccccgac ccccgacccc cgacccacga tccacgatcc + 1081 acgatcacag atcagatcca cgatcccgca tgtgccttgt tagatcctcg tccgatgctt + 1141 cacgcttcta tattcgtgat tcgtgatttg tgaattttct tcctttccgt gttcgtgttt + 1201 cctcgccttg cacacgcagg ttgcgcagat cattcatgat tgaaagacaa agcacaccaa + 1261 ccctaaaatc tgagtcgtag gtgtcgtgtg gagcacctcg gtcggtaccg cagctcttgg + 1321 cacttgtcgt ccatcgacca acttgaatcg cacacaccgc actcttgcat ggtctgaatc + 1381 gacggcctag atggccacca tggcgtgaca attgcggccg ccgcgaagct tgccggcaga + 1441 tccgatgtac ccgtaccgag ctcgactttc acttttctct atcactgata gggagtggta + 1501 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt + 1561 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga + 1621 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc + 1681 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga + 1741 tagggagtgg taaactcgag taccgagctc gactttcact tttctctatc actgataggg + 1801 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt + 1861 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg + 1921 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc + 1981 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta + 2041 tcactgatag ggagtggtaa actcgagggg atcaattcga ccaatgaggc gcgagacgag + 2101 gggacgctgg aagttgaggc gcaagaaaat ttttctctgg ttctgcgcgg cagagacgac + 2161 cagattcgcc cgctttcttc tgcgttgggt gcctcttttg ggtgccagac tttgtgtgtg + 2221 cgccagcgag acgttccaat aaagggcgct gtctcggcac tatctttctt tctttcctca + 2281 tacatcgtat cataccatac acagacaaca tcatccacgg gatcgcacgc gtaggatcat + 2341 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc + 2401 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca + 2461 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg + 2521 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg + 2581 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag + 2641 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg + 2701 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc + 2761 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa + 2821 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac + 2881 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat + 2941 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac + 3001 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc + 3061 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt + 3121 gacgagttct tctgagcggg actctggggt tcgaaatcgt ccccttttcc tttgtcgata + 3181 tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg + 3241 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt + 3301 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt + 3361 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt + 3421 taatttgcaa gctgtaggag tgcggccgca ttaataggcc tgagtggcca tggtcccata + 3481 cgggtttcgt caactctatg ttcaatagac tgggattctt tcggtgcgcc ccggctgctg + 3541 tgatgatgaa tatgaattgt tcgtgtggat caacagcgtg tatgtactgt tgtgtgtact + 3601 gtactcacga ccgtaaatat cccgtctcgt ttctgttgtg tgtgtagacc atggacaaaa + 3661 actacagtaa gcgtgttagt ccttgctgtt cgaacaaggg tttggaaatt cacgattttt + 3721 ggtatgcacg atgtgccgtg ggcgcgtgac gcgttgagac caacaaaatc acgaatagac + 3781 cctttcacgt ttctcctcca ccaactcacg actcgaatgt ttggagcatt ggaagcacct + 3841 tggaagctcg tattgttcgg ccacgacgca gtaactctgg atcctatctg tacattgcat + 3901 catcacgggc gacgctgctt gatcaagcct tttctccgac gtggtcaaca ctttgctcct + 3961 tcactgctgc cgacatcgcg ttgagacatt tccagctatc aagtcgattc gacaccacac + 4021 tctttgtcaa cttggcatca tctctctttc actcacaatg tcgtcagagg cgcaaactac + 4081 gccagaagct cacaatgcag atgaacaact tgtgatggag caggagagag tggatgagga + 4141 gagagagaca tcgtttcagt ttcagaagca agcacgcgat gcgccggtgc aagcttctaa + 4201 ttcggcgtta ggtgaagttg gagagagttt gggtgatgaa gcattgcaaa cgcattcagc + 4261 gacggaaatg gcgacggagg gtaaggaaca aggggaagca gaagcggaag cgaaagcggt + 4321 ggaggaagcg acggcggcgg tgaaggagga gacgagtagc aagccaacgc gcagtgggag + 4381 aaagagtcga acatcgacgg atgcacctac agcatccgag gagcaagttc gaaagaagtc + 4441 acgccgatcc aatgctccgc catccacatc tgaagctggc ttgggaaccg acgtcaacgt + 4501 ccggctcgac cgactgcgtc aagcgctctt ccgcttcctc gacctgatcg aacaccgcgc + 4561 cacgcgctcc tcgttcgcca aagcgctccc tcacatcgac gccgactcgg tagaagccct + 4621 gcgtcagcag tttgtcagtc aactcaaaga cgccatcatc gacgaatccg aaaaactgat + 4681 cgattcaaac gatctacaag ccaaattggc ctcgttgcac cgattgaccc aggaagcaga + 4741 cgccaggtac caggcaggat acgcagagaa tgccccagag atcaaggacg tttggagaaa + 4801 agagattgat ctcgaaaccg ccatctcggc aaaggccatt ccggaccagg agaggagagt + 4861 cgatcaattg aagctagaat tggattcagt gcgcaagcag aatcgtgcca tccatgctgc + 4921 attgctcgag acgcgtgcaa gaagcactgc gattcagcgt gatgcaaccg actcattgga + 4981 cgctctcgaa tcagtaagtc attctcaaca cacaccccgt gttattcact tcaaaacatc + 5041 acgcactgac ctccatcctc gtgtccacct cgtgctccca aaacaggcaa tcaaaggtct + 5101 cgaagctacg ccagacatgc aaaagcaact tcgccaaacc atggacgacc tcctccaaga + 5161 cctcggcgcg cgggtctaac gatcaccgtc acctcacctg tacatcagct tcacagacct + 5221 ttctctctcg gcgcgatctg cacgtttagc ttttactcat tgaaagagca atgagattct + 5281 gctcgtgttg aacggcagac accagtggcg ctaagggtat agcaaaacag gaatattaag + 5341 ggcgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc + 5401 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac + 5461 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc + 5521 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat + 5581 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca + 5641 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct + 5701 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt + 5761 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac + 5821 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct + 5881 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt + 5941 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac + 6001 aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa + 6061 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc + 6121 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac + 6181 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg + 6241 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc + 6301 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg + 6361 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt + 6421 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc + 6481 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact + 6541 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt + 6601 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca + 6661 ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat + 6721 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg + 6781 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga + 6841 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga + 6901 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa + 6961 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga + 7021 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt + 7081 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct + 7141 tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc + 7201 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg + 7261 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc + 7321 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact + 7381 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc + 7441 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag + 7501 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat + 7561 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt + 7621 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa + 7681 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc + 7741 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg + 7801 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt + 7861 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca + 7921 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat + 7981 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca + 8041 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg + 8101 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg + 8161 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt + 8221 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg + 8281 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata + 8341 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca + 8401 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag + 8461 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc + 8521 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga + 8581 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg + 8641 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac + 8701 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg + 8761 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg + 8821 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct + 8881 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc + 8941 gagcgcagcg agtcagtgag cgaggaagcg gaag +// +LOCUS pUMa2187 5235 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Fragment 2 into bb +ACCESSION pUMa2187 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5235) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: bb + Fragment ends: EcoRI and BamHI + Fragment size: 4938 + Insert molecule: Fragment 2 + Fragment ends: BamHI and EcoRI + Fragment size: 297 +FEATURES Location/Qualifiers + CDS 258..911 + /gene="GST" + /SECDrawAs="Gene" + CDS 258..1226 + /gene="GST-MLLE_Pab1" + /SECDrawAs="Gene" + CDS 918..935 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 918..935 + /gene="Thrombine site" + /SECDrawAs="Label" + CDS 954..1226 + /gene="MLLE_pab1" + /SECDrawAs="Gene" + misc_signal 957..1148 + /gene="MLLE" + /SECDrawAs="Label" + misc_feature 957..1148 + /gene="MLLE" + /SECDrawAs="Region" + CDS 1643..2503 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3707..4666 + /gene="ORF-2" + /SECDrawAs="Gene" +ORIGIN + 1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg + 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt + 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc + 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca + 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc + 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc + 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc + 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata + 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc + 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact + 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag + 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt + 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa + 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat + 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc + 901 atcctccaaa atcggatctg gttccgcgtg gatcccatat ggtggccatt acggccagtc + 961 ccgaggagca gaagcagatg cttggtgagg ccatctaccc caaggttgcc gccagccagc + 1021 ccgagcttgc cggtaagctt accggtatga tccttgagct tcccgtcact gaactcctgc + 1081 accttctcga ggagagcgag gcgctggatg caaaggtcaa cgaggctctc gaggttctca + 1141 aggagtacca acagaacgac tctgcgggtg ctgaagccga agctaacgcc gaggctccca + 1201 agactgaggc taaggccaac gcgtgagaat tcatcgtgac tgactgacga tctgcctcgc + 1261 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc + 1321 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg + 1381 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt ataattcttg + 1441 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt + 1501 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt + 1561 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca + 1621 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt + 1681 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga + 1741 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa + 1801 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct + 1861 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat + 1921 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga + 1981 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc + 2041 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat + 2101 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa + 2161 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac + 2221 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa + 2281 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc + 2341 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc + 2401 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag + 2461 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta + 2521 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa + 2581 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc + 2641 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat + 2701 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga + 2761 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt + 2821 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata + 2881 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac + 2941 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg + 3001 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg + 3061 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag + 3121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct + 3181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc + 3241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt + 3301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg + 3361 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga + 3421 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg + 3481 cggtatttca caccgcataa attccgacac catcgaatgg tgcaaaacct ttcgcggtat + 3541 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt + 3601 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca + 3661 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa + 3721 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt + 3781 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg + 3841 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc + 3901 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta + 3961 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt + 4021 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg + 4081 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc + 4141 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac + 4201 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt + 4261 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa + 4321 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc + 4381 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt + 4441 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca + 4501 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag + 4561 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt + 4621 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta + 4681 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta + 4741 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt + 4801 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa + 4861 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc + 4921 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt + 4981 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact + 5041 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgta + 5101 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac + 5161 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt + 5221 gatggcgttg gaatt +// +LOCUS pUMa2985 10660 bp DNA circular SYN 14-AUG-2024 +DEFINITION product rotated to position 8274 +ACCESSION pUMa2985 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 10660) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: kate + Fragment ends: SfiI and AscI + Fragment size: 756 + Insert molecule: bb + Fragment ends: AscI and SfiI + Fragment size: 9904 +FEATURES Location/Qualifiers + CDS 2..2377 + /gene="rrm4" + /SECDrawAs="Gene" + misc_feature 220..424 + /gene="RRM1" + /SECDrawAs="Region" + misc_signal 335..358 + /gene="RNP1" + /SECDrawAs="Label" + misc_feature 472..640 + /gene="RRM2" + /SECDrawAs="Region" + misc_signal 593..616 + /gene="RNP1" + /SECDrawAs="Label" + misc_feature 967..1183 + /gene="RRM3" + /SECDrawAs="Region" + misc_signal 1082..1105 + /gene="RNP1" + /SECDrawAs="Label" + misc_feature 2181..2380 + /gene="MLLE3" + /SECDrawAs="Region" + CDS 2396..3097 + /gene="mKate2" + /SECDrawAs="Gene" + CDS 3110..3136 + /gene="HA-tag" + /SECDrawAs="Gene" + misc_feature 3149..3441 + /gene="Tnos" + /SECDrawAs="Region" + misc_feature 3452..4829 + /gene="NatR" + /product="nourseotricine resistance cassette" + /SECDrawAs="Region" + misc_feature 4855..6841 + /gene="DF" + /product="RB aus pUMa430" + /SECDrawAs="Region" + misc_signal 5244..5244 + /gene="vermutlich: A-->C" + /SECDrawAs="Label" + misc_feature 5244 + /gene="vermutlich A-->C" + /SECDrawAs="Marker" + CDS 5381..5742 + /gene="um10835" + /product="related to cytochrome b561" + /SECDrawAs="Gene" + misc_feature 5740 + /gene="mutierte AS" + /product="in genom. Sequenz zwei Adenine!! + Punktmutation" + /SECDrawAs="Marker" + misc_signal 5740..5742 + /gene="mutierte AS" + /product="in genom. Sequenz zwei Adenine!! + Punktmutation" + /SECDrawAs="Label" + misc_feature 6096 + /gene="vermutlich: A-->G" + /SECDrawAs="Marker" + misc_signal 6096..6096 + /gene="vermutlich: A-->G" + /SECDrawAs="Label" + CDS 8135..8995 + /gene="amp" + /SECDrawAs="Gene" + misc_feature 9832..1 + /gene="UF" + /product="Left border" + /SECDrawAs="Region" + misc_feature 10357 + /gene="2nt Deletion" + /product="2 a deletion= 14 real statt 16 virtuell, + genomisch: 15 Adenine" + /SECDrawAs="Marker" +ORIGIN + 1 catgtccgat tcgatttacg ccccacataa taagcacaag ctcgaggcag cgcgagccgc + 61 tgatgctgcc gccgatgatg ccgccaccgt cagcgccctc gtcgagccga ccgactccac + 121 tgcgcaggca agtcacgctg cggagcaaac cattgatgcg catcagcaag caggtgacgt + 181 cgaacctgaa cgatgccatc ctcacctcac tcgccccttg ctctacctta gcggtgtcga + 241 tgccaccatg actgacaagg agctcgccgg ccttgtcttt gatcaagtac tccccgtccg + 301 gctcaaaatt gaccgcaccg tgggcgaggg ccagacagcc agcggcaccg tcgagttcca + 361 gacgctggat aaggccgaga aggcttatgc taccgttcgt cctccgatcc agctgcgcat + 421 caaccaggac gcctccatca gagagccgca cccctcggcc aaaccacgtc ttgtcaagca + 481 gctgccacct acatcggacg acgcgttcgt atacgacctc ttccgtcctt tcggtccgct + 541 ccgacgagct cagtgtctgc tcaccaatcc agccggcatt cacaccggct tcaagggtat + 601 ggccgtgctt gaattctact ctgaacaaga cgcccagcgt gccgaatccg agatgcactg + 661 cagcgaagtc ggcggcaagt cgatctctgt tgccattgat accgctacgc gcaaggtcag + 721 cgctgctgca gccgagttcc gtccctcggc tgctgcattc gttccagcgg gcagcatgag + 781 tccgtcagct ccgtcctttg acccttaccc tgctggctct cgttccgttt cgacaggctc + 841 agccgcttcc atctacgcaa cttctggcgc cgctccgact cacgataccc gcaacggtgc + 901 tcagaaggga gcgcgtgttc cgctccagta ttcgagccaa gcatctacgt atgtcgaccc + 961 ttgcaacctc ttcatcaaga acctcgaccc caacatggaa tccaatgacc tcttcgacac + 1021 cttcaaaagg tttggccaca tcgtctctgc acgcgtcatg cgcgacgata acggcaagag + 1081 cagagaattt gggtttgtca gcttcaccac tcctgacgaa gcgcaacaag ctctacaagc + 1141 aatggacaat gccaaacttg gcaccaaaaa gatcattgtc cgtctgcacg agcccaagac + 1201 gatgcgtcag gagaagctcg cagcgcgtta caacgctgcc aatgccgata acagtgacat + 1261 gagcagcaac agtccgccca cagaggctcg caaagccgac aagcgtcaaa gcagaagcta + 1321 cttcaaagca ggtgtgcctt cggatgccag cggtcttgta gacgaagagc aattgcgctc + 1381 gctcagcact gtcgtgagaa acgagctctt gtctggcgaa tttacacgaa gaatccccaa + 1441 ggtttcctcg gtcaccgagg cgcagttgga cgacgttgta ggcgagctgc tcagtctcaa + 1501 gcttgctgat gccgtcgagg cgctcaataa cccaatttcg cttattcaaa ggatcagcga + 1561 tgctcgcgag cagcttgcac agaagtctgc ctccactctg acggctccga gtccggcgcc + 1621 actgtctgct gaacacccag ctatgctcgg tattcaggct cagcgtagcg tatcaagcgc + 1681 atcttcgacc ggcgaaggag gtgcgtcggt caaggagagg gagaggctac tcaaggctgt + 1741 catctccgtc accgaaagtg gtgcccccgt tgaggacatc accgacatga tcgcaagcct + 1801 gccaaagaag gatcgtgccc ttgcgctctt caatccagag tttctcaagc aaaaagtgga + 1861 cgaagccaag gacattctcg acatcaccga cgaaagtggc gaagatcttt cgcctcctcg + 1921 tgcttccagt gggagcgctc cggtgccatt gtcagtgcag acgcctgctt ctgctatttt + 1981 caaagatgcc agcaacggcc agagctccat ctcgcctggc gctgctgagg cttacacatt + 2041 atccacgctt gctgcgcttc ctgcagccga gattgtgcgc ttggccaact cccaatccag + 2101 ctccggtctg cctctcccta aagcggatcc agcgacggtc aaggcaaccg acgatttcat + 2161 cgactcgctt cagggcaaag cagcgcacga tcagaagcag aagctgggtg atcagctctt + 2221 caaaaagatc cgtacgttcg gcgtcaaagg cgcacccaag ctcaccatcc acttgttgga + 2281 ttccgaagac ttgcgggcgc tggcgcatct catgaactcg tacgaggacg tgctcaagga + 2341 aaaagttcag cacaaggttg ctgcaggtct gaacaaggcg gccaacgcgg ccaccatggt + 2401 gtcggagctc atcaaggaga acatgcacat gaagctctac atggagggca ccgtcaacaa + 2461 ccaccacttc aagtgcacct cggaaggcga gggcaagccc tacgagggca cccagaccat + 2521 gcgcatcaag gccgtcgagg gtggtcctct gcccttcgcc ttcgacatcc tcgccacctc + 2581 gttcatgtac ggctccaaga cgttcatcaa ccacacgcag ggcatccccg actttttcaa + 2641 gcagtcgttc cccgagggct tcacctggga gcgtgtcacc acctacgagg acggcggtgt + 2701 cctcaccgcc acccaggaca cctcgctcca ggacggctgc ctcatctaca acgtcaagat + 2761 ccgcggtgtc aacttcccct cgaacggtcc cgtcatgcag aagaagacgc tcggctggga + 2821 ggcctcgacc gagacgctct accccgccga cggtggcctc gagggtcgtg ccgacatggc + 2881 gctcaagctc gtcggtggtg gtcacctcat ctgcaacctc aagaccacct accgctccaa + 2941 gaagcccgcc aagaacctca agatgcccgg tgtctactac gtcgaccgtc gtctcgagcg + 3001 catcaaggag gccgacaagg aaacctacgt cgagcagcac gaggtcgccg tcgctcgcta + 3061 ctgcgatctg ccctcgaaac tcggtcaccg ccatatgcag ggtccgctct acccttacga + 3121 cgtgcccgac tacgcctgag cggcgcgccg gccgcccggc gatcgttcaa acatttggca + 3181 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct + 3241 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg + 3301 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata + 3361 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atccgatgat + 3421 aagctgtcaa acatgagaat tgcggccgca cctagaagtg ataagcggaa agaatctgac + 3481 ttgctgtgat cagcagccaa tttttttttc gttttttttt tttcactcca catcgtcgtg + 3541 cgtgcacggt ctgcatgtgt aaattgtatt catcgaaagc cacagttgaa tacatcagcc + 3601 cgatgtggat ttcgaaaacc aattaatctt ggaattcacg cgctcagatc agtccataga + 3661 gtcgacttcg gctgtttcca agagcttctt ctctgcgagg tggttgcccg tgtttctcgc + 3721 tgggaaaaaa ggatcgatta ttattcgctt ctacctcgct cgcacccatg gcctgctgaa + 3781 ggaaacagcg ccgagactcg gtcacggttg ctgggctccg tgttgatgct gggacggcgc + 3841 aaagtggggc ccgcgcactc ttcgagccaa ggacctcact cttcaagaac aagcgctgtc + 3901 gccatcgtct tcttctttct gctccaccat cgaatctttc tttctcgttt cgaaaccaaa + 3961 acactcttcc atatgaccac tcttgacgac acggcttacc ggtaccgcac cagtgtcccg + 4021 ggggacgccg aggccatcga ggcactggat gggtccttca ccaccgacac cgtcttccgc + 4081 gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc cggtggaccc gcccctgacc + 4141 aaggtgttcc ccgacgacga atcggacgac gaatcggacg acggggagga cggcgacccg + 4201 gactcccgga cgttcgtcgc gtacggggac gacggcgacc tggcgggctt cgtggtcatc + 4261 tcgtactcgg cgtggaaccg ccggctgacc gtcgaggaca tcgaggtcgc cccggagcac + 4321 cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc cggcgagcgg + 4381 ggcgccgggc acctctggct ggaggtcacc aacgtcaacg caccggcgat ccacgcgtac + 4441 cggcggatgg ggttcaccct ctgcggcctg gacaccgccc tgtacgacgg caccgcctcg + 4501 gacggcgagc ggcaggcgct ctacatgagc atgccctgcc cctagatcgt ccccttttcc + 4561 tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc + 4621 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt + 4681 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac + 4741 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc + 4801 tcgaaggctt taatttgcaa gctgtaggag tgcggccgca ttaataggcc tgagtggcca + 4861 aaggtcgaac tggcggccgc tgtggtccct agatctctct gttctccgag tgacaaccag + 4921 acatatgttc cactgctacc gttgtaggat tgtaccaagg atgtggagct gaggtaaagt + 4981 gcggctatac acacgcttaa tgtgcggtgc agcaagtcgt gattgggcta cacacgtggt + 5041 cgcggcggat gagcaagctc cgaatcacga atcagaatgt cgcaatttgt gcgcgcgaat + 5101 cacgattgcg gttttcacgt tttttgacag gcggccaagc agccacgaga ttcacgatta + 5161 tctgatttgg tcagcataga cggcatggta tgtctttcaa cggacaggtt tcgagtgtag + 5221 catttcagcg cttgttcgag tttacgtcga tcaacacgca cgctctccac tgttatcgat + 5281 ggtccaaact agttctgagg ttctctgagc tgactggtcg acatcgtagc ggggttccga + 5341 aggaaagact tggtctcacg gcacaaacac aagcgaagcc atgttgcaag gtcgcgcgag + 5401 cgagcaggga tccaccacgc aaccactgct gaccaccaca aacgacgact accaccatca + 5461 cgagacagac gccttgatca cgccgctcga catggttcgg catgaaaacg tctcgcgcca + 5521 tgcggtgggc gtgcaagttt ccgcgttgct ctttgtcgtc gttgtatgga gcattgtttt + 5581 ctccactatg tcgatccttt cgctgccact cttcggctat catccattga tccagtcttt + 5641 caccatactg cttctgctgc aggcgattgt agtactgcag cggacgacgc catcacaacc + 5701 agcggcgaaa aatcggcgtt cacagcacac cagtggatcg tcctcattct cgtactccca + 5761 ctcttcaccg cgggcgcaag catcatgtgg tacctgcacg atcagccagc ctcggcacac + 5821 ttcatatcgt accatgggat tctcggtacc gccgtcgtaa tcgcagcgtg ggtacaggcc + 5881 gccttcggtg ccgcaagcgt ctggtggaag ggaaagctag tgggtggaga gaacaaaggt + 5941 aaggcgcttt ggaagtggca tagattgtct ggctatgtac tcattgcgct gtttctcttg + 6001 acggctgtgt tgggtgtggt cgagactacg tgggggaagc ggaatgcccg gccgatccag + 6061 aagggcgtga cggtggtgac gttaagtttg gcggcatttg cgatgctcat ccgcgtgcaa + 6121 aagagcaaac tgccaaagct gtagtcttcc aaccgcgctt aggtgtaatt ctaccatatt + 6181 cttggaccgt gtctatgctt gccgtgcaat aaactctgca attgtcaact atgaccaaga + 6241 tcatacctgc tgcaaacgtt gtccggcttt atcaagatgc gtctgaatcg actcagcagc + 6301 tcgtcaagct cacgactaat ctgcttcgtt tcaaagcatg aaggcttgaa caagaatgca + 6361 ggaccatgtc ggcctcctca actctcccca accttgccac tatcccgcaa aaggctggtc + 6421 aaagaccgag agccagtgcc tgacgtcaaa gaaatgacta caacaacagc aagcttccac + 6481 agaagcatgc atggacgctg acaagcctcc gctgggtcaa caagtgccac aaagcgaaag + 6541 acgggggtta ttaaggtgtg aattgaaggg cgcgtgcagt ttaggcatac atattcaatt + 6601 gaaaaaaggg ggcccgacaa gcatatgttc gcgaatagat gaggtaggga tggagggtat + 6661 caatacgcca tagtataaag agaatcctca ctgcctggca ccccacaggt gagccaagtc + 6721 aaagagggga atgttaaagg gaaagtcggt ggcggcgaga cacagatgaa gcagaatgta + 6781 gaagccaccc atgcaagcag tgaaaccaca agcaccggcg gcggtgatca ggttggcacc + 6841 gaagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagc + 6901 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct + 6961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac + 7021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac + 7081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc + 7141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg + 7201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc + 7261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt + 7321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc + 7381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa + 7441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc + 7501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg + 7561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc + 7621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc + 7681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca + 7741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact + 7801 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg + 7861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt + 7921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct + 7981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga + 8041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa + 8101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac + 8161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga + 8221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc + 8281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca + 8341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta + 8401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg + 8461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc + 8521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg + 8581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt + 8641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt + 8701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata + 8761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc + 8821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac + 8881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa + 8941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct + 9001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat + 9061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc + 9121 cacactgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc + 9181 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt + 9241 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc + 9301 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt + 9361 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt + 9421 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt + 9481 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa + 9541 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc + 9601 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga + 9661 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac + 9721 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta + 9781 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcgccct tgcacgcatc + 9841 cacagtccac gatcgtgcat agcagatttg tagagtcgtg tgttgaggta aagacgttca + 9901 cagtttcatg attcacaaag ctaacacgaa tcgtgaatca cattaacatc gttaagtagc + 9961 agataatcgt aattgtgaat ctgtgatttg gtttaagtac gtgcgaaaac acataagtta + 10021 cacactatca acatcacaaa cacggccttt gtgacacgca atggctacca cgacggcaaa + 10081 attcacagat ttgatatctg tagcctattg atgtggacag agattgacct cgatcttgat + 10141 cttgacctgg tcaacttttg tggtcctctt acttgtgctt atcttgaacc ttcatgagtg + 10201 tggcaaggtg gcagaaggag ggtgacatgc cctaggtgcg ctgaactctc caccgcggcc + 10261 acacgtgaca ccacgcagtg cgtgtgttac tttcgcttcc tttgagatct ttcttggaag + 10321 aacaagctca agttgaggtt cacagaccca aaaaccaaaa aaaaaaaaaa aatcgtgaat + 10381 gaaaggcggc gtgtgcatgc gtgtgtattg catgacgatc atttcaacgc tcgtcttgaa + 10441 tcgtttgagt acgaagcggc atggcagctc gcactccgaa agagactgtg caactcacta + 10501 gagcccgtct tcggaagatc cgtctgcgac tctgctgccc acctgtagtt tagcgtcgtg + 10561 atcctcgaca ttgtctcacg accaagacga cgacaacaac aacaacaacg acaacgtttc + 10621 ggctcaagtc ggaaccttgc cgctctgctt taaaggtcat +// +LOCUS pUMa3908 11287 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert_SfiI%_Rrm4 into VBB from p3587 with SfiI% +ACCESSION pUMa3908 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 11287) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB from p3587 with SfiI% + Fragment ends: SfiI + Fragment size: 8896 + Insert molecule: Insert_SfiI%_Rrm4 + Fragment ends: SfiI + Fragment size: 2391 +FEATURES Location/Qualifiers + CDS complement (146..406) + /gene="lacZ" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_feature 417..1441 + /gene="UF" + /SECDrawAs="Region" + CDS 1458..4592 + /gene="Rrm4_mKate_HA" + /product="MF220+MF844 auf pUMa992%ClaI" + /SECDrawAs="Gene" + CDS 1458..3833 + /gene="rrm4" + /product="rrm4 ORF" + /SECDrawAs="Gene" + misc_feature 1676..1880 + /gene="RRM1" + /SECDrawAs="Region" + misc_feature 1928..2096 + /gene="RRM2" + /SECDrawAs="Region" + CDS 2160..2270 + /gene="3xPAM2 of Rrm4?" + /SECDrawAs="Gene" + misc_signal 2160..2270 + /gene="3xPAM2 of Rrm4?" + /SECDrawAs="Label" + misc_feature 2423..2639 + /gene="RRM3" + /SECDrawAs="Region" + misc_feature 2808..3059 + /gene="MLLE1" + /SECDrawAs="Region" + misc_signal 2808..3059 + /gene="MLLE1" + /SECDrawAs="Label" + misc_feature 3177..3335 + /gene="MLLE2" + /SECDrawAs="Region" + misc_signal 3177..3335 + /gene="MLLE2" + /SECDrawAs="Label" + CDS 3252..3302 + /gene="PAm2_II of Rrm4?" + /SECDrawAs="Gene" + misc_feature 3637..3833 + /gene="MLLE3 (PABC)" + /SECDrawAs="Region" + misc_signal 3834..3848 + /gene="Linker" + /SECDrawAs="Label" + CDS 3849..4592 + /gene="mKate2_HA" + /SECDrawAs="Gene" + misc_feature 4563..4589 + /gene="HA-tag" + /SECDrawAs="Region" + misc_feature 4597..4890 + /gene="Tnos" + /product="nos-terminator" + /SECDrawAs="Region" + CDS 4895..7570 + /gene="HygR" + /product="Hygromycin resistance casette" + /SECDrawAs="Gene" + misc_feature 4897..5445 + /gene="hsp70T" + /SECDrawAs="Region" + misc_feature 6775..7570 + /gene="hsp70P" + /SECDrawAs="Region" + misc_feature 7598..8993 + /gene="DF" + /SECDrawAs="Region" + misc_signal 7750..7750 + /gene="G to C" + /product="mutated to remove NdeI" + /SECDrawAs="Label" + CDS 8204..8968 + /gene="cytochrome b561" + /product="UMAG_10835" + /SECDrawAs="Gene" + misc_signal 8997..9000 + /gene="'BsaI DF" + /SECDrawAs="Label" + CDS complement (9003..9070) + /gene="lacZ'" + /product="beta-galactosidase" + /SECDrawAs="Gene" + misc_signal 10371..10371 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (10227..11087) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" +ORIGIN + 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca + 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg + 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc + 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc + 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat + 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt + 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcacc tgcaatatta + 421 gtttcggggt catcatggga caaacatgca accggcgtga ggcttgcggt ttggggacat + 481 tcgtgattta cgatgcacga ttcacgattc acgattcacg attcacgatt cgtgatatct + 541 gattggagca ggtgcagtcc aagcgatcta acaaggagtt tatgcctcac cccttacctc + 601 tcacgcctcg ccgcacgcat ccacagtcca cgatcgtgca tagcagattt gtagagtcgt + 661 gtgttgaggt aaagacgttc acagtttcat gattcacaaa gctaacacga atcgtgaatc + 721 acattaacat cgttaagtag cagataatcg taattgtgaa tctgtgattt ggtttaagta + 781 cgtgcgaaaa cacataagtt acacactatc aacatcacaa acacggcctt tgtgacacgc + 841 aatggctacc acgacggcaa aattcacaga tttgatatct gtagcctatt gatgtggaca + 901 gagattgacc tcgatcttga tcttgacctg gtcaactttt gtggtcctct tacttgtgct + 961 tatcttgaac cttcatgagt gtggcaaggt ggcagaagga gggtgacatg ccctaggtgc + 1021 gctgaactct ccaccgcggc cacacgtgac accacgcagt gcgtgtgtta ctttcgcttc + 1081 ctttgagatc tttcttggaa gaacaagctc aagttgaggt tcacagaccc aaaaaccaaa + 1141 aaaaaaaaaa aatcgtgaat gaaaggcggc gtgtgcatgc gtgtgtattg catgacgatc + 1201 atttcaacgc tcgtcttgaa tcgtttgagt acgaagcggc atggcagctc gcactccgaa + 1261 agagactgtg caactcacta gagcccgtct tcggaagatc cgtctgcgac tctgctgccc + 1321 acctgtagtt tagcgtcgtg atcctcgaca ttgtctcacg accaagacga cgacaacaac + 1381 aacaacaacg acaacgtttc ggctcaagtc ggaaccttgc cgctctgctt taaaggtcat + 1441 cggcctagaa ggcccatatg tccgattcga tttacgcccc acataataag cacaagctcg + 1501 aggcagcgcg agccgctgat gctgccgccg atgatgccgc caccgtcagc gccctcgtcg + 1561 agccgaccga ctccactgcg caggcaagtc acgctgcgga gcaaaccatt gatgcgcatc + 1621 agcaagcagg tgacgtcgaa cctgaacgat gccatcctca cctcactcgc cccttgctct + 1681 accttagcgg tgtcgatgcc accatgactg acaaggagct cgccggcctt gtctttgatc + 1741 aagtactccc cgtccggctc aaaattgacc gcaccgtggg cgagggccag acagccagcg + 1801 gcaccgtcga gttccagacg ctggataagg ccgagaaggc ttatgctacc gttcgtcctc + 1861 cgatccagct gcgcatcaac caggacgcct ccatcagaga gccgcacccc tcggccaaac + 1921 cacgtcttgt caagcagctg ccacctacat cggacgacgc gttcgtatac gacctcttcc + 1981 gtcctttcgg tccgctccga cgagctcagt gtctgctcac caatccagcc ggcattcaca + 2041 ccggcttcaa gggtatggcc gtgcttgaat tctactctga acaagacgcc cagcgtgccg + 2101 aatccgagat gcactgcagc gaagtcggcg gcaagtcgat ctctgttgcc attgataccg + 2161 ctacgcgcaa ggtcagcgct gctgcagccg agttccgtcc ctcggctgct gcattcgttc + 2221 cagcgggcag catgagtccg tcagctccgt cctttgaccc ttaccctgct ggctctcgtt + 2281 ccgtttcgac aggctcagcc gcttccatct acgcaacttc tggcgccgct ccgactcacg + 2341 atacccgcaa cggtgctcag aagggagcgc gtgttccgct ccagtattcg agccaagcat + 2401 ctacgtatgt cgacccttgc aacctcttca tcaagaacct cgaccccaac atggaatcca + 2461 atgacctctt cgacaccttc aaaaggtttg gccacatcgt ctctgcacgc gtcatgcgcg + 2521 acgataacgg caagagcaga gaatttgggt ttgtcagctt caccactcct gacgaagcgc + 2581 aacaagctct acaagcaatg gacaatgcca aacttggcac caaaaagatc attgtccgtc + 2641 tgcacgagcc caagacgatg cgtcaggaga agctcgcagc gcgttacaac gctgccaatg + 2701 ccgataacag tgacatgagc agcaacagtc cgcccacaga ggctcgcaaa gccgacaagc + 2761 gtcaaagcag aagctacttc aaagcaggtg tgccttcgga tgccagcggt cttgtagacg + 2821 aagagcaatt gcgctcgctc agcactgtcg tgagaaacga gctcttgtct ggcgaattta + 2881 cacgaagaat ccccaaggtt tcctcggtca ccgaggcgca gttggacgac gttgtaggcg + 2941 agctgctcag tctcaagctt gctgatgccg tcgaggcgct caataaccca atttcgctta + 3001 ttcaaaggat cagcgatgct cgcgagcagc ttgcacagaa gtctgcctcc actctgacgg + 3061 ctccgagtcc ggcgccactg tctgctgaac acccagctat gctcggtatt caggctcagc + 3121 gtagcgtatc aagcgcatct tcgaccggcg aaggaggtgc gtcggtcaag gagagggaga + 3181 ggctactcaa ggctgtcatc tccgtcaccg aaagtggtgc ccccgttgag gacatcaccg + 3241 acatgatcgc aagcctgcca aagaaggatc gtgcccttgc gctcttcaat ccagagtttc + 3301 tcaagcaaaa agtggacgaa gccaaggaca ttctcgacat caccgacgaa agtggcgaag + 3361 atctttcgcc tcctcgtgct tccagtggga gcgctccggt gccattgtca gtgcagacgc + 3421 ctgcttctgc tattttcaaa gatgccagca acggccagag ctccatctcg cctggcgctg + 3481 ctgaggctta cacattatcc acgcttgctg cgcttcctgc agccgagatt gtgcgcttgg + 3541 ccaactccca atccagctcc ggtctgcctc tccctaaagc ggatccagcg acggtcaagg + 3601 caaccgacga tttcatcgac tcgcttcagg gcaaagcagc gcacgatcag aagcagaagc + 3661 tgggtgatca gctcttcaaa aagatccgta cgttcggcgt caaaggcgca cccaagctca + 3721 ccatccactt gttggattcc gaagacttgc gggcgctggc gcatctcatg aactcgtacg + 3781 aggacgtgct caaggaaaaa gttcagcaca aggttgctgc aggtctgaac aaggccaacg + 3841 cggccaccat ggtgtcggag ctcatcaagg agaacatgca catgaagctc tacatggagg + 3901 gcaccgtcaa caaccaccac ttcaagtgca cctcggaagg cgagggcaag ccctacgagg + 3961 gcacccagac catgcgcatc aaggccgtcg agggtggtcc tctgcccttc gccttcgaca + 4021 tcctcgccac ctcgttcatg tacggctcca agacgttcat caaccacacg cagggcatcc + 4081 ccgacttttt caagcagtcg ttccccgagg gcttcacctg ggagcgtgtc accacctacg + 4141 aggacggcgg tgtcctcacc gccacccagg acacctcgct ccaggacggc tgcctcatct + 4201 acaacgtcaa gatccgcggt gtcaacttcc cctcgaacgg tcccgtcatg cagaagaaga + 4261 cgctcggctg ggaggcctcg accgagacgc tctaccccgc cgacggtggc ctcgagggtc + 4321 gtgccgacat ggcgctcaag ctcgtcggtg gtggtcacct catctgcaac ctcaagacca + 4381 cctaccgctc caagaagccc gccaagaacc tcaagatgcc cggtgtctac tacgtcgacc + 4441 gtcgtctcga gcgcatcaag gaggccgaca aggaaaccta cgtcgagcag cacgaggtcg + 4501 ccgtcgctcg ctactgcgat ctgccctcga aactcggtca ccgccatatg cagggtccgc + 4561 tctaccctta cgacgtgccc gactacgcct gagcggcgcg ccggccgccc ggcgatcgtt + 4621 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta + 4681 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt + 4741 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag + 4801 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac + 4861 tagatccgat gataagctgt caaacatgag aattgcggcc gcactcgagt ggcggcagat + 4921 gtgagtcgtg tgctacaaaa cgtgaatcga cacgcgcagg gcggaaccaa aaaaaacccc + 4981 caccccgctg tcaagttgac aaatcaacac atttgttcca agttcaaatc gggaaatcaa + 5041 aaattagggc cagatcagcg atcaggaatg gtaatcggta acagaggtcg caaaatcgtc + 5101 tagaaagtgg aagaagaacg tggtaactac cagcgagttc tgcaaacttc aaaaaaaaaa + 5161 tctgggcacg atgaaagttg agctaacgct gacgctcaca aatggcgtgg ctaaaggaag + 5221 cgagacaatc ggaaaattgt tctctcgggc accacaaagc tgttgttagt cgctgaagaa + 5281 caattccaac tgattccgcc gccttcctat tgcgtcagcc ttgtacctaa gctgccgagt + 5341 aacgtcactc aacctctctt ttcagactgc tttgctccgc gaatactttt cttctatgcg + 5401 ctcaagaaaa tgacacagca caccaagctc tgcaaacttt cttcgctaat ctgacgcgaa + 5461 atgtgagcca tttcttctcg cctgcaatgg caatgcgtct gtgcggcgat gagaatcacg + 5521 atgcggaatg ggtggctgga agttcataga gatgctgagt tgttggagcg acatggtaca + 5581 taagcatgag tctgtcctga tttccaccct cccgtctttc atcaactttc tcgtctgacc + 5641 cttccgttgc cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg + 5701 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg + 5761 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg + 5821 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg + 5881 gccgcgctcc cgattccgga agtgcttgac attggggaac tcagcgagag cctgacctat + 5941 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc + 6001 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag + 6061 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat + 6121 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc + 6181 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc + 6241 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc + 6301 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc + 6361 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc + 6421 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt + 6481 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg + 6541 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc + 6601 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga + 6661 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgggatct + 6721 gggcagcacc aagacacgga acttcaacag tcgtgagtag gtggtgatct gcatgtccaa + 6781 atcgtgaaac gtgaatcacg aaccacgaat cgtgaatagc cactttgcgc gaaaatcact + 6841 gaatgccgcc aaaccgacgc gacgcgagtt gcgtgaagta ttcgtgattc acattcacga + 6901 ttgacgattg tgagttggtc gacgacattc gctagccaga ccaacactca cgactttttg + 6961 aagagcgttg caggctgagc caactttctc cctcactctt tgctttcttc tccatcacgc + 7021 tcattccttt taagtgttca tccggtatca ttagcactat atcatagtga aactcgcaac + 7081 aacccggtct tcgtcgcgaa agcaaccctc catcgctgct gcctcttgca gtgaagaccg + 7141 ccaccatgca gtctacgcag tcgaagaccc tacacgtcca gttcgtccat tctggtccta + 7201 cacattgtat ccacgcaccc aagtctgcca acgtggagta agtgaacctt tctttgtcct + 7261 gtcgaggctc aacgtagatc acaggctgat catccacact cttctacctt ttgacattac + 7321 gcagtcgact ttttacagga ggagatgact acctcgtccg aatcttaccc accctgcctc + 7381 tctccgacgt agagccgcag cttatcgaag acgccactga agcggtgacc tcgctcgatg + 7441 ctgacggccg cttcctcgtc accgcttccg aagatggtgt caacgcctct accgacacca + 7501 cccaatcgac gcggaaggca acccagcatc accggctgtg cttcaatcgc tgctgcgccg + 7561 cgaagctgtg cggccgcatt aataggcctg agtggccgaa tcacgaatgt gcacattgtc + 7621 actagggcgt gcgtggtaat gacaccttcc gcgtagttgg ttcgtgttaa caagcgttat + 7681 ataaaggtcg aactggcggc cgctgtggtc cctagatctc tctgttctcc gagtgacaac + 7741 cagacatatc ttccactgct accgttgtag gattgtacca aggatgtgga gctgaggtaa + 7801 agtgcggcta tacacacgct taatgtgcgg tgcagcaagt cgtgattggg ctacacacgt + 7861 ggtcgcggcg gatgagcaag ctccgaatca cgaatcagaa tgtcgcaatt tgtgcgcgcg + 7921 aatcacgatt gcggttttca cgttttttga caggcggcca agcagccacg agattcacga + 7981 ttatctgatt tggtcagcat agacggcatg gtatgtcttt caacggacag gtttcgagtg + 8041 tagcatttca gcgcttgttc gagtttacgt cgatcaacac gcacgctctc cactgttatc + 8101 gatggtccaa actagttctg aggttctctg agctgactgg tcgacatcgt agcggggttc + 8161 cgaaggaaag acttgctctc acggcacaaa cacaagcgaa gccatgttgc aaggtcgcgc + 8221 gagcgagcag ggatccacca cgcaaccact gctgaccacc acaaacgacg actaccacca + 8281 tcacgagaca gacgccttga tcacgccgct cgacatggtt cggcatgaaa acgtctcgcg + 8341 ccatgcggtg ggcgtgcaag tttccgcgtt gctctttgtc gtcgttgtat ggagcattgt + 8401 tttctccact atgtcgatcc tttcgctgcc actcttcggc tatcatccat tgatccagtc + 8461 tttcaccata ctgcttctgc tgcaggcgat tgtagtactg cagcggacga cgccatcaca + 8521 accagcggcg aaaaaatcgg cgttcacagc acaccagtgg atcaacctca ttctcgtact + 8581 cccactcttc accgcgggcg caagcatcat gtggtacctg cacgatcaac cagcctcggc + 8641 acacttcata tcgtaccatg ggattctcgg taccgccgtc gtaatcgcag cgtgggtaca + 8701 ggccgccttc ggtgccgcaa gcgtctggtg gaagggaaag ctagtgggtg gagagaacaa + 8761 aggtaaggcg ctttggaagt ggcatagatt gtctggctat gtactcattg cgctgtttct + 8821 cttgacggct gtgttgggtg tggtcgagac tacgtggggg aagcggaatg cccggccgat + 8881 ccagaagggc gtgacggtgg tgacgttaag tttggcggca tttgcgatgc tcatccgcgt + 8941 gcaaaagagc aaactgccaa agctgtagtc ttccaaccgc gcttaggtgt aatattgcag + 9001 gtctagatat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa + 9061 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata + 9121 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta + 9181 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa + 9241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg + 9301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag + 9361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa + 9421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc + 9481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca + 9541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg + 9601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct + 9661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt + 9721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag + 9781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc + 9841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac + 9901 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga + 9961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc + 10021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg + 10081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca + 10141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt + 10201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca + 10261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg + 10321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca + 10381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt + 10441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt + 10501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca + 10561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca + 10621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga + 10681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact + 10741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga + 10801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg + 10861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc + 10921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga + 10981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat + 11041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt + 11101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt + 11161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac + 11221 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc + 11281 tttcgtc +// +LOCUS pUMa4296 5623 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert4296 into p4295% +ACCESSION pUMa4296 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5623) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p4295% + Fragment ends: XhoI and NdeI + Fragment size: 5210 + Insert molecule: Insert4296 + Fragment ends: NdeI and XhoI + Fragment size: 413 +FEATURES Location/Qualifiers + source 162..456 + /gene="source''" + /SECDrawAs="Info only" + source 457..818 + /gene="''source'" + /SECDrawAs="Info only" + CDS 458..868 + /gene="n6xHis_SUMO_PAM2" + /SECDrawAs="Gene" + CDS 458..815 + /gene="His_SUMO" + /SECDrawAs="Gene" + CDS 458..868 + /gene="N6his-SUMO-PAM2" + /SECDrawAs="Gene" + CDS 470..487 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 497..514 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 497..514 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 521..817 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 521..814 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 815..865 + /gene="PAM2" + /SECDrawAs="Gene" + source 870..161 + /gene="'source" + /SECDrawAs="Info only" + CDS complement (1572..2387) + /gene="KanR" + /product="aminoglycoside phosphotransferase" + /SECDrawAs="Gene" + misc_feature 3283..3422 + /gene="bom" + /product="basis of mobility region from pBR322" + /SECDrawAs="Region" + CDS complement (3524..3715) + /gene="rop" + /product="Rop protein, which maintains plasmids at low + copy number" + /SECDrawAs="Gene" + CDS complement (4524..5606) + /gene="lacI" + /product="lac repressor" + /SECDrawAs="Gene" +ORIGIN + 1 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt + 61 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg + 121 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac + 181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg + 241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca + 301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc + 361 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag + 421 aaataatttt gtttaacttt aagaaggaga tatacatatg ggcagcagcc atcatcatca + 481 tcatcacggc agcggcctgg tgccgcgcgg cagcgctagc atgtcggact cagaagtcaa + 541 tcaagaagct aagccagagg tcaagccaga agtcaagcct gagactcaca tcaatttaaa + 601 ggtgtccgat ggatcttcag agatcttctt caagatcaaa aagaccactc ctttaagaag + 661 gctgatggaa gcgttcgcta aaagacaggg taaggaaatg gactccttaa gattcttgta + 721 cgacggtatt agaattcaag ctgatcagac ccctgaagat ttggacatgg aggataacga + 781 tattattgag gctcacagag aacagattgg tggttcgcaa agcaccttgt ctcccaacgc + 841 ctccgtcttc aagccgtccc gatcctaact cgagtgagat ccggctgcta acaaagcccg + 901 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc + 961 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa + 1021 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg + 1081 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc + 1141 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga + 1201 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt + 1261 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat + 1321 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat + 1381 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa + 1441 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa + 1501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca + 1561 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca + 1621 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg + 1681 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca + 1741 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca + 1801 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt + 1861 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc + 1921 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa + 1981 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct + 2041 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt + 2101 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc + 2161 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca + 2221 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct + 2281 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa + 2341 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta + 2401 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt + 2461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt + 2521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg + 2581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca + 2641 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt + 2701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga + 2761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc + 2821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact + 2881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga + 2941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg + 3001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt + 3061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt + 3121 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga + 3181 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac + 3241 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct + 3301 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct + 3361 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg + 3421 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat + 3481 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt + 3541 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt + 3601 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg + 3661 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg + 3721 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga + 3781 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta + 3841 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc + 3901 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga + 3961 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga + 4021 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc + 4081 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta + 4141 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga + 4201 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga + 4261 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa + 4321 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga + 4381 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc + 4441 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct + 4501 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc + 4561 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg + 4621 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc + 4681 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg + 4741 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc + 4801 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc + 4861 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg + 4921 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt + 4981 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt + 5041 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc + 5101 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca + 5161 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca + 5221 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc + 5281 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt + 5341 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg + 5401 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg + 5461 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg + 5521 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg + 5581 acatcgtata acgttactgg tttcacattc accaccctga att +// +LOCUS pUMa4297 5626 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of insert into p4295 % +ACCESSION pUMa4297 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5626) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p4295 % + Fragment ends: XhoI and NdeI + Fragment size: 5210 + Insert molecule: insert + Fragment ends: NdeI and XhoI + Fragment size: 416 +FEATURES Location/Qualifiers + source 162..456 + /gene="source''" + /SECDrawAs="Info only" + TATA_signal 370..386 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + source 457..815 + /gene="''source'" + /SECDrawAs="Info only" + CDS 458..871 + /gene="N6xHis_Sumo_PAM2L1" + /SECDrawAs="Gene" + CDS 470..487 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_feature 497..514 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 497..514 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_signal 521..814 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 521..814 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 815..868 + /gene="PAM2L1" + /SECDrawAs="Gene" + source 873..161 + /gene="'source" + /SECDrawAs="Info only" + CDS 930..948 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Gene" + CDS complement (1575..2390) + /gene="KanR" + /product="aminoglycoside phosphotransferase" + /SECDrawAs="Gene" + misc_feature 3286..3425 + /gene="bom" + /product="basis of mobility region from pBR322" + /SECDrawAs="Region" + CDS complement (3527..3718) + /gene="rop" + /product="Rop protein, which maintains plasmids at low + copy number" + /SECDrawAs="Gene" + CDS complement (4527..5609) + /gene="lacI" + /product="lac repressor" + /SECDrawAs="Gene" +ORIGIN + 1 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt + 61 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg + 121 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac + 181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg + 241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca + 301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc + 361 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag + 421 aaataatttt gtttaacttt aagaaggaga tatacatatg ggcagcagcc atcatcatca + 481 tcatcacggc agcggcctgg tgccgcgcgg cagcgctagc atgtcggact cagaagtcaa + 541 tcaagaagct aagccagagg tcaagccaga agtcaagcct gagactcaca tcaatttaaa + 601 ggtgtccgat ggatcttcag agatcttctt caagatcaaa aagaccactc ctttaagaag + 661 gctgatggaa gcgttcgcta aaagacaggg taaggaaatg gactccttaa gattcttgta + 721 cgacggtatt agaattcaag ctgatcagac ccctgaagat ttggacatgg aggataacga + 781 tattattgag gctcacagag aacagattgg tggtgaggct gcagaccaag aggaagatca + 841 agatgacttt gtctacccag gagccgacta actcgagtga gatccggctg ctaacaaagc + 901 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg + 961 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc + 1021 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc + 1081 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt + 1141 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc + 1201 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt + 1261 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt + 1321 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt + 1381 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa + 1441 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg + 1501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc + 1561 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata + 1621 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca + 1681 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca + 1741 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca + 1801 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact + 1861 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta + 1921 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta + 1981 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca + 2041 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg + 2101 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat + 2161 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg + 2221 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca + 2281 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg + 2341 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt + 2401 gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga + 2461 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc + 2521 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt + 2581 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc + 2641 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc + 2701 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg + 2761 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg + 2821 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga + 2881 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc + 2941 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg + 3001 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg + 3061 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt + 3121 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc + 3181 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg + 3241 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt + 3301 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc + 3361 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg + 3421 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg + 3481 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac + 3541 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg + 3601 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta + 3661 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg + 3721 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga + 3781 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg + 3841 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc + 3901 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc + 3961 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac + 4021 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc + 4081 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc + 4141 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg + 4201 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag + 4261 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc + 4321 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca + 4381 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg + 4441 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga + 4501 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt + 4561 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc + 4621 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg + 4681 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt + 4741 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact + 4801 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc + 4861 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc + 4921 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga + 4981 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa + 5041 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg + 5101 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag + 5161 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg + 5221 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc + 5281 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc + 5341 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga + 5401 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg + 5461 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa + 5521 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct + 5581 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaatt +// +LOCUS pUMa4298 5626 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert 4298 NdeI, XhoI % into p4295_BB_NdeI, XhoI % +ACCESSION pUMa4298 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5626) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p4295_BB_NdeI, XhoI % + Fragment ends: XhoI and NdeI + Fragment size: 5210 + Insert molecule: Insert 4298 NdeI, XhoI % + Fragment ends: NdeI and XhoI + Fragment size: 416 +FEATURES Location/Qualifiers + source 162..456 + /gene="source''" + /SECDrawAs="Info only" + misc_feature 370..386 + /gene="T7P" + /SECDrawAs="Region" + misc_signal 370..386 + /gene="T7P" + /SECDrawAs="Label" + source 457..815 + /gene="''source'" + /SECDrawAs="Info only" + CDS 458..871 + /gene="N6His_TS_SUMO_PAM2LC" + /SECDrawAs="Gene" + CDS 470..487 + /gene="6xHis" + /product="6xHis affinity tag" + /SECDrawAs="Region" + misc_signal 497..514 + /gene="Thrombine site" + /SECDrawAs="Label" + misc_feature 497..514 + /gene="Thrombine site" + /SECDrawAs="Region" + misc_signal 521..814 + /gene="SUMO Protein" + /SECDrawAs="Label" + CDS 521..814 + /gene="SUMO" + /product="cleavable ubiquitin-like protein tag" + /SECDrawAs="Gene" + CDS 815..868 + /gene="PAM2L-C" + /SECDrawAs="Gene" + misc_feature 815..868 + /gene="PAM2L-C" + /SECDrawAs="Region" + misc_signal 869..871 + /gene="STOP" + /SECDrawAs="Label" + source 873..161 + /gene="'source" + /SECDrawAs="Info only" + misc_signal 930..948 + /gene="T7T" + /SECDrawAs="Label" + misc_feature 930..948 + /gene="T7T" + /SECDrawAs="Region" + CDS complement (1575..2390) + /gene="KanR" + /product="aminoglycoside phosphotransferase" + /SECDrawAs="Gene" + misc_feature 3286..3425 + /gene="bom" + /product="basis of mobility region from pBR322" + /SECDrawAs="Region" + CDS complement (3527..3718) + /gene="rop" + /product="Rop protein, which maintains plasmids at low + copy number" + /SECDrawAs="Gene" + CDS complement (4527..5609) + /gene="lacI" + /product="lac repressor" + /SECDrawAs="Gene" +ORIGIN + 1 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt + 61 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg + 121 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac + 181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg + 241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca + 301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc + 361 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag + 421 aaataatttt gtttaacttt aagaaggaga tatacatatg ggcagcagcc atcatcatca + 481 tcatcacggc agcggcctgg tgccgcgcgg cagcgctagc atgtcggact cagaagtcaa + 541 tcaagaagct aagccagagg tcaagccaga agtcaagcct gagactcaca tcaatttaaa + 601 ggtgtccgat ggatcttcag agatcttctt caagatcaaa aagaccactc ctttaagaag + 661 gctgatggaa gcgttcgcta aaagacaggg taaggaaatg gactccttaa gattcttgta + 721 cgacggtatt agaattcaag ctgatcagac ccctgaagat ttggacatgg aggataacga + 781 tattattgag gctcacagag aacagattgg tggtgacgag gatgcggccg atgacgatga + 841 cgatgaattc atctacccga actcgtacta actcgagtga gatccggctg ctaacaaagc + 901 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg + 961 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc + 1021 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc + 1081 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt + 1141 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc + 1201 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt + 1261 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt + 1321 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt + 1381 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa + 1441 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg + 1501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc + 1561 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata + 1621 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca + 1681 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca + 1741 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca + 1801 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact + 1861 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta + 1921 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta + 1981 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca + 2041 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg + 2101 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat + 2161 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg + 2221 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca + 2281 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg + 2341 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt + 2401 gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga + 2461 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc + 2521 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt + 2581 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc + 2641 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc + 2701 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg + 2761 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg + 2821 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga + 2881 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc + 2941 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg + 3001 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg + 3061 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt + 3121 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc + 3181 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg + 3241 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt + 3301 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc + 3361 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg + 3421 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg + 3481 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac + 3541 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg + 3601 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta + 3661 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg + 3721 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga + 3781 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg + 3841 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc + 3901 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc + 3961 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac + 4021 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc + 4081 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc + 4141 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg + 4201 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag + 4261 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc + 4321 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca + 4381 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg + 4441 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga + 4501 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt + 4561 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc + 4621 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg + 4681 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt + 4741 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact + 4801 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc + 4861 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc + 4921 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga + 4981 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa + 5041 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg + 5101 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag + 5161 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg + 5221 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc + 5281 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc + 5341 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga + 5401 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg + 5461 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa + 5521 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct + 5581 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaatt +// +LOCUS pUMa4435 10987 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert_p4435_AgeI+SnaBI% into p3909_SnaBI+AgeI_VBB% +ACCESSION pUMa4435 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 10987) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: p3909_SnaBI+AgeI_VBB% + Fragment ends: AgeI and SnaBI + Fragment size: 9102 + Insert molecule: Insert_p4435_AgeI+SnaBI% + Fragment ends: SnaBI and AgeI + Fragment size: 1886 +FEATURES Location/Qualifiers + misc_feature 417..1441 + /gene="UF" + /SECDrawAs="Region" + CDS 1457..3521 + /gene="Rrm4_Del_MLLE3" + /SECDrawAs="Gene" + CDS 1457..4337 + /gene="Rrm4_Del_MLLE3_mKate" + /SECDrawAs="Gene" + CDS 1458..3593 + /gene="Rrm4_Del_MLLE3_HA_HRV3C" + /product="rrm4 ORF" + /SECDrawAs="Gene" + misc_feature 1676..1880 + /gene="RRM1" + /SECDrawAs="Region" + misc_signal 1928..2096 + /gene="RRM2" + /SECDrawAs="Label" + misc_feature 1928..2096 + /gene="RRM2" + /SECDrawAs="Region" + misc_signal 2169..2222 + /gene="2x PAM2!_1" + /SECDrawAs="Label" + misc_feature 2169..2222 + /gene="2x PAM2!_1" + /SECDrawAs="Region" + misc_feature 2226..2270 + /gene="PAM2!_2" + /SECDrawAs="Region" + misc_signal 2226..2270 + /gene="PAM2!_2" + /SECDrawAs="Label" + misc_feature 2423..2639 + /gene="RRM3" + /SECDrawAs="Region" + misc_signal 2538..2561 + /gene="RNP1" + /SECDrawAs="Label" + misc_feature 2808..3059 + /gene="MLLE1" + /SECDrawAs="Region" + misc_signal 2808..3059 + /gene="MLLE1" + /SECDrawAs="Label" + misc_signal 3177..3335 + /gene="MLLE2" + /SECDrawAs="Label" + misc_feature 3177..3335 + /gene="MLLE2" + /SECDrawAs="Region" + misc_feature 3522..3548 + /gene="HA_tag" + /SECDrawAs="Region" + misc_signal 3522..3548 + /gene="HA tag" + /SECDrawAs="Label" + misc_signal 3549..3572 + /gene="HRV3CP/ Precision site" + /product="HRV3CP/ Precision protease cleavage site" + /SECDrawAs="Label" + misc_feature 3549..3572 + /gene="HRV3CP/ Precision site" + /product="HRV3CP/ Precision protease site" + /SECDrawAs="Region" + CDS 3594..4288 + /gene="mKate2" + /SECDrawAs="Gene" + misc_feature 4297..4590 + /gene="Tnos" + /product="nos-terminator" + /SECDrawAs="Region" + CDS 4595..7270 + /gene="HygR" + /product="Hygromycin resistance casette" + /SECDrawAs="Gene" + misc_feature 4597..5145 + /gene="hsp70T" + /SECDrawAs="Region" + misc_feature 6475..7270 + /gene="hsp70P" + /SECDrawAs="Region" + misc_feature 7298..8693 + /gene="DF" + /SECDrawAs="Region" + misc_signal 7450..7450 + /gene="G to C" + /product="mutated to remove NdeI" + /SECDrawAs="Label" + CDS 7904..8668 + /gene="cytochrome b561" + /product="UMAG_10835" + /SECDrawAs="Gene" + misc_signal 8697..8700 + /gene="BsaI DF" + /SECDrawAs="Label" + misc_signal 10071..10071 + /gene="BsaI*" + /product="g zu t" + /SECDrawAs="Label" + CDS complement (9927..10787) + /gene="AmpR* woBsaI" + /product="beta-lactamase (Ampizillin Resistenz) bla Gen" + /SECDrawAs="Gene" +ORIGIN + 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca + 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg + 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc + 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc + 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat + 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt + 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcacc tgcaatatta + 421 gtttcggggt catcatggga caaacatgca accggcgtga ggcttgcggt ttggggacat + 481 tcgtgattta cgatgcacga ttcacgattc acgattcacg attcacgatt cgtgatatct + 541 gattggagca ggtgcagtcc aagcgatcta acaaggagtt tatgcctcac cccttacctc + 601 tcacgcctcg ccgcacgcat ccacagtcca cgatcgtgca tagcagattt gtagagtcgt + 661 gtgttgaggt aaagacgttc acagtttcat gattcacaaa gctaacacga atcgtgaatc + 721 acattaacat cgttaagtag cagataatcg taattgtgaa tctgtgattt ggtttaagta + 781 cgtgcgaaaa cacataagtt acacactatc aacatcacaa acacggcctt tgtgacacgc + 841 aatggctacc acgacggcaa aattcacaga tttgatatct gtagcctatt gatgtggaca + 901 gagattgacc tcgatcttga tcttgacctg gtcaactttt gtggtcctct tacttgtgct + 961 tatcttgaac cttcatgagt gtggcaaggt ggcagaagga gggtgacatg ccctaggtgc + 1021 gctgaactct ccaccgcggc cacacgtgac accacgcagt gcgtgtgtta ctttcgcttc + 1081 ctttgagatc tttcttggaa gaacaagctc aagttgaggt tcacagaccc aaaaaccaaa + 1141 aaaaaaaaaa aatcgtgaat gaaaggcggc gtgtgcatgc gtgtgtattg catgacgatc + 1201 atttcaacgc tcgtcttgaa tcgtttgagt acgaagcggc atggcagctc gcactccgaa + 1261 agagactgtg caactcacta gagcccgtct tcggaagatc cgtctgcgac tctgctgccc + 1321 acctgtagtt tagcgtcgtg atcctcgaca ttgtctcacg accaagacga cgacaacaac + 1381 aacaacaacg acaacgtttc ggctcaagtc ggaaccttgc cgctctgctt taaaggtcat + 1441 cggcctagaa ggcccatatg tccgattcga tttacgcccc acataataag cacaagctcg + 1501 aggcagcgcg agccgctgat gctgccgccg atgatgccgc caccgtcagc gccctcgtcg + 1561 agccgaccga ctccactgcg caggcaagtc acgctgcgga gcaaaccatt gatgcgcatc + 1621 agcaagcagg tgacgtcgaa cctgaacgat gccatcctca cctcactcgc cccttgctct + 1681 accttagcgg tgtcgatgcc accatgactg acaaggagct cgccggcctt gtctttgatc + 1741 aagtactccc cgtccggctc aaaattgacc gcaccgtggg cgagggccag acagccagcg + 1801 gcaccgtcga gttccagacg ctggataagg ccgagaaggc ttatgctacc gttcgtcctc + 1861 cgatccagct gcgcatcaac caggacgcct ccatcagaga gccgcacccc tcggccaaac + 1921 cacgtcttgt caagcagctg ccacctacat cggacgacgc gttcgtatac gacctcttcc + 1981 gtcctttcgg tccgctccga cgagctcagt gtctgctcac caatccagcc ggcattcaca + 2041 ccggcttcaa gggtatggcc gtgcttgaat tctactctga acaagacgcc cagcgtgccg + 2101 aatccgagat gcactgcagc gaagtcggcg gcaagtcgat ctctgttgcc attgataccg + 2161 ctacgcgcaa ggtcagcgct gctgcagccg agttccgtcc ctcggctgct gcattcgttc + 2221 cagcgggcag catgagtccg tcagctccgt cctttgaccc ttaccctgct ggctctcgtt + 2281 ccgtttcgac aggctcagcc gcttccatct acgcaacttc tggcgccgct ccgactcacg + 2341 atacccgcaa cggtgctcag aagggagcgc gtgttccgct ccagtattcg agccaagcat + 2401 ctacgtatgt cgacccttgc aacctcttca tcaagaacct cgaccccaac atggaatcca + 2461 atgacctctt cgacaccttc aaaaggtttg gccacatcgt ctctgcacgc gtcatgcgcg + 2521 acgataacgg caagagcaga gaatttgggt ttgtcagctt caccactcct gacgaagcgc + 2581 aacaagctct acaagcaatg gacaatgcca aacttggcac caaaaagatc attgtccgtc + 2641 tgcacgagcc caagacgatg cgtcaggaga agctcgcagc gcgttacaac gctgccaatg + 2701 ccgataacag tgacatgagc agcaacagtc cgcccacaga ggctcgcaaa gccgacaagc + 2761 gtcaaagcag aagctacttc aaagcaggtg tgccttcgga tgccagcggt cttgtagacg + 2821 aagagcaatt gcgctcgctc agcactgtcg tgagaaacga gctcttgtct ggcgaattta + 2881 cacgaagaat ccccaaggtt tcctcggtca ccgaggcgca gttggacgac gttgtaggcg + 2941 agctgctcag tctcaagctt gctgatgccg tcgaggcgct caataaccca atttcgctta + 3001 ttcaaaggat cagcgatgct cgcgagcagc ttgcacagaa gtctgcctcc actctgacgg + 3061 ctccgagtcc ggcgccactg tctgctgaac acccagctat gctcggtatt caggctcagc + 3121 gtagcgtatc aagcgcatct tcgaccggcg aaggaggtgc gtcggtcaag gagagggaga + 3181 ggctactcaa ggctgtcatc tccgtcaccg aaagtggtgc ccccgttgag gacatcaccg + 3241 acatgatcgc aagcctgcca aagaaggatc gtgcccttgc gctcttcaat ccagagtttc + 3301 tcaagcaaaa agtggacgaa gccaaggaca ttctcgacat caccgacgaa agtggcgaag + 3361 atctttcgcc tcctcgtgct tccagtggga gcgctccggt gccattgtca gtgcagacgc + 3421 ctgcttctgc tattttcaaa gatgccagca acggccagag ctccatctcg cctggcgctg + 3481 ctgaggctta cacattatcc acgcttgctg cgcttcctgc atacccttac gacgtgcccg + 3541 actacgccct ggaagtactg ttccaggggc ccgcggccaa cgcggccacc atggtgtcgg + 3601 agctcatcaa ggagaacatg cacatgaagc tctacatgga gggcaccgtc aacaaccacc + 3661 acttcaagtg cacctcggaa ggcgagggca agccctacga gggcacccag accatgcgca + 3721 tcaaggccgt cgagggtggt cctctgccct tcgccttcga catcctcgcc acctcgttca + 3781 tgtacggctc caagacgttc atcaaccaca cgcagggcat ccccgacttt ttcaagcagt + 3841 cgttccccga gggcttcacc tgggagcgtg tcaccaccta cgaggacggc ggtgtcctca + 3901 ccgccaccca ggacacctcg ctccaggacg gctgcctcat ctacaacgtc aagatccgcg + 3961 gtgtcaactt cccctcgaac ggtcccgtca tgcagaagaa gacgctcggc tgggaggcct + 4021 cgaccgagac gctctacccc gccgacggtg gcctcgaggg tcgtgccgac atggcgctca + 4081 agctcgtcgg tggtggtcac ctcatctgca acctcaagac cacctaccgc tccaagaagc + 4141 ccgccaagaa cctcaagatg cccggtgtct actacgtcga ccgtcgtctc gagcgcatca + 4201 aggaggccga caaggaaacc tacgtcgagc agcacgaggt cgccgtcgct cgctactgcg + 4261 atctgccctc gaaactcggt caccctaaac cggtggcgcg ccggccgccc ggcgatcgtt + 4321 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta + 4381 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt + 4441 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag + 4501 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac + 4561 tagatccgat gataagctgt caaacatgag aattgcggcc gcactcgagt ggcggcagat + 4621 gtgagtcgtg tgctacaaaa cgtgaatcga cacgcgcagg gcggaaccaa aaaaaacccc + 4681 caccccgctg tcaagttgac aaatcaacac atttgttcca agttcaaatc gggaaatcaa + 4741 aaattagggc cagatcagcg atcaggaatg gtaatcggta acagaggtcg caaaatcgtc + 4801 tagaaagtgg aagaagaacg tggtaactac cagcgagttc tgcaaacttc aaaaaaaaaa + 4861 tctgggcacg atgaaagttg agctaacgct gacgctcaca aatggcgtgg ctaaaggaag + 4921 cgagacaatc ggaaaattgt tctctcgggc accacaaagc tgttgttagt cgctgaagaa + 4981 caattccaac tgattccgcc gccttcctat tgcgtcagcc ttgtacctaa gctgccgagt + 5041 aacgtcactc aacctctctt ttcagactgc tttgctccgc gaatactttt cttctatgcg + 5101 ctcaagaaaa tgacacagca caccaagctc tgcaaacttt cttcgctaat ctgacgcgaa + 5161 atgtgagcca tttcttctcg cctgcaatgg caatgcgtct gtgcggcgat gagaatcacg + 5221 atgcggaatg ggtggctgga agttcataga gatgctgagt tgttggagcg acatggtaca + 5281 taagcatgag tctgtcctga tttccaccct cccgtctttc atcaactttc tcgtctgacc + 5341 cttccgttgc cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg + 5401 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg + 5461 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg + 5521 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg + 5581 gccgcgctcc cgattccgga agtgcttgac attggggaac tcagcgagag cctgacctat + 5641 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc + 5701 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag + 5761 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat + 5821 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc + 5881 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc + 5941 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc + 6001 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc + 6061 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc + 6121 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt + 6181 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg + 6241 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc + 6301 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga + 6361 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgggatct + 6421 gggcagcacc aagacacgga acttcaacag tcgtgagtag gtggtgatct gcatgtccaa + 6481 atcgtgaaac gtgaatcacg aaccacgaat cgtgaatagc cactttgcgc gaaaatcact + 6541 gaatgccgcc aaaccgacgc gacgcgagtt gcgtgaagta ttcgtgattc acattcacga + 6601 ttgacgattg tgagttggtc gacgacattc gctagccaga ccaacactca cgactttttg + 6661 aagagcgttg caggctgagc caactttctc cctcactctt tgctttcttc tccatcacgc + 6721 tcattccttt taagtgttca tccggtatca ttagcactat atcatagtga aactcgcaac + 6781 aacccggtct tcgtcgcgaa agcaaccctc catcgctgct gcctcttgca gtgaagaccg + 6841 ccaccatgca gtctacgcag tcgaagaccc tacacgtcca gttcgtccat tctggtccta + 6901 cacattgtat ccacgcaccc aagtctgcca acgtggagta agtgaacctt tctttgtcct + 6961 gtcgaggctc aacgtagatc acaggctgat catccacact cttctacctt ttgacattac + 7021 gcagtcgact ttttacagga ggagatgact acctcgtccg aatcttaccc accctgcctc + 7081 tctccgacgt agagccgcag cttatcgaag acgccactga agcggtgacc tcgctcgatg + 7141 ctgacggccg cttcctcgtc accgcttccg aagatggtgt caacgcctct accgacacca + 7201 cccaatcgac gcggaaggca acccagcatc accggctgtg cttcaatcgc tgctgcgccg + 7261 cgaagctgtg cggccgcatt aataggcctg agtggccgaa tcacgaatgt gcacattgtc + 7321 actagggcgt gcgtggtaat gacaccttcc gcgtagttgg ttcgtgttaa caagcgttat + 7381 ataaaggtcg aactggcggc cgctgtggtc cctagatctc tctgttctcc gagtgacaac + 7441 cagacatatc ttccactgct accgttgtag gattgtacca aggatgtgga gctgaggtaa + 7501 agtgcggcta tacacacgct taatgtgcgg tgcagcaagt cgtgattggg ctacacacgt + 7561 ggtcgcggcg gatgagcaag ctccgaatca cgaatcagaa tgtcgcaatt tgtgcgcgcg + 7621 aatcacgatt gcggttttca cgttttttga caggcggcca agcagccacg agattcacga + 7681 ttatctgatt tggtcagcat agacggcatg gtatgtcttt caacggacag gtttcgagtg + 7741 tagcatttca gcgcttgttc gagtttacgt cgatcaacac gcacgctctc cactgttatc + 7801 gatggtccaa actagttctg aggttctctg agctgactgg tcgacatcgt agcggggttc + 7861 cgaaggaaag acttgctctc acggcacaaa cacaagcgaa gccatgttgc aaggtcgcgc + 7921 gagcgagcag ggatccacca cgcaaccact gctgaccacc acaaacgacg actaccacca + 7981 tcacgagaca gacgccttga tcacgccgct cgacatggtt cggcatgaaa acgtctcgcg + 8041 ccatgcggtg ggcgtgcaag tttccgcgtt gctctttgtc gtcgttgtat ggagcattgt + 8101 tttctccact atgtcgatcc tttcgctgcc actcttcggc tatcatccat tgatccagtc + 8161 tttcaccata ctgcttctgc tgcaggcgat tgtagtactg cagcggacga cgccatcaca + 8221 accagcggcg aaaaaatcgg cgttcacagc acaccagtgg atcaacctca ttctcgtact + 8281 cccactcttc accgcgggcg caagcatcat gtggtacctg cacgatcaac cagcctcggc + 8341 acacttcata tcgtaccatg ggattctcgg taccgccgtc gtaatcgcag cgtgggtaca + 8401 ggccgccttc ggtgccgcaa gcgtctggtg gaagggaaag ctagtgggtg gagagaacaa + 8461 aggtaaggcg ctttggaagt ggcatagatt gtctggctat gtactcattg cgctgtttct + 8521 cttgacggct gtgttgggtg tggtcgagac tacgtggggg aagcggaatg cccggccgat + 8581 ccagaagggc gtgacggtgg tgacgttaag tttggcggca tttgcgatgc tcatccgcgt + 8641 gcaaaagagc aaactgccaa agctgtagtc ttccaaccgc gcttaggtgt aatattgcag + 8701 gtctagatat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa + 8761 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata + 8821 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta + 8881 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa + 8941 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg + 9001 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag + 9061 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa + 9121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc + 9181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca + 9241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg + 9301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct + 9361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt + 9421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag + 9481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc + 9541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac + 9601 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga + 9661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc + 9721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg + 9781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca + 9841 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt + 9901 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca + 9961 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg + 10021 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca + 10081 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt + 10141 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt + 10201 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca + 10261 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca + 10321 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga + 10381 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact + 10441 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga + 10501 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg + 10561 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc + 10621 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga + 10681 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat + 10741 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt + 10801 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt + 10861 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac + 10921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc + 10981 tttcgtc +// +LOCUS pUMa4701 5269 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of p4701 insert into VBB +ACCESSION pUMa4701 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5269) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB + Fragment ends: XhoI and NcoI + Fragment size: 4964 + Insert molecule: p4701 insert + Fragment ends: NcoI and XhoI + Fragment size: 305 +FEATURES Location/Qualifiers + CDS 295..948 + /gene="GST" + /SECDrawAs="Gene" + CDS 295..1275 + /gene="GST_Rrm4_MLLE3(700-792)" + /SECDrawAs="Gene" + CDS 955..971 + /gene="Thrombine site" + /SECDrawAs="Gene" + misc_signal 955..971 + /gene="Thrombine cleavage site" + /SECDrawAs="Label" + CDS 992..1275 + /gene="Rrm4_MLLE3(700-792AA)" + /SECDrawAs="Gene" + misc_feature 1076..1272 + /gene="MLLE3" + /SECDrawAs="Region" + misc_signal 1076..1272 + /gene="MLLE3" + /SECDrawAs="Label" + CDS 1714..2574 + /gene="AmpR" + /SECDrawAs="Gene" + CDS 3778..4737 + /gene="lacI" + /SECDrawAs="Gene" +ORIGIN + 1 aggccagacg cgaattattt ttgatggcgt tggaattacg ttatcgactg cacggtgcac + 61 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat + 121 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg + 181 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc + 241 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagt attcatgtcc + 301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa + 361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga + 421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat + 481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg + 541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt + 601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt + 661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat + 721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt + 781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt + 841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct + 901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt + 961 ccgcgtggat ccatgggagc tagcgcggcc gccagctccg gtctgcctct ccctaaagcg + 1021 gatccagcga cggtcaaggc aaccgacgat ttcatcgact cgcttcaggg caaagcagcg + 1081 cacgatcaga agcagaagct gggtgatcag ctcttcaaaa agatccgtac gttcggcgtc + 1141 aaaggcgcac ccaagctcac catccacttg ttggattccg aagacttgcg ggcgctggcg + 1201 catctcatga actcgtacga ggacgtgctc aaggaaaaag ttcagcacaa ggttgctgca + 1261 ggtctgaaca agtgactcga ggccaacgcg gcctagtgaa ttcatcgtga ctgactgacg + 1321 atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag + 1381 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca + 1441 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg + 1501 tataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg + 1561 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct + 1621 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga + 1681 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc + 1741 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg + 1801 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc + 1861 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact + 1921 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc + 1981 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag + 2041 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat + 2101 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt + 2161 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa + 2221 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc + 2281 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg + 2341 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt + 2401 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca + 2461 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat + 2521 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca + 2581 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg + 2641 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg + 2701 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt + 2761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg + 2821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata + 2881 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca + 2941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag + 3001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc + 3061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga + 3121 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg + 3181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac + 3241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg + 3301 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg + 3361 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct + 3421 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc + 3481 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt + 3541 acgcatctgt gcggtatttc acaccgcata aattccgaca ccatcgaatg gtgcaaaacc + 3601 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa + 3661 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc + 3721 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg + 3781 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg + 3841 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg + 3901 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc + 3961 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg + 4021 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat + 4081 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc + 4141 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc + 4201 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat + 4261 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc + 4321 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg + 4381 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg + 4441 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat + 4501 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac + 4561 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca + 4621 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg + 4681 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg + 4741 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct + 4801 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta + 4861 tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg + 4921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg + 4981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct + 5041 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg + 5101 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct + 5161 acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga + 5221 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacagga +// +LOCUS pUMa4704 5733 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of PCR4704% into pUMa3626-pET22b vector +ACCESSION pUMa4704 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5733) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: pUMa3626-pET22b vector + Fragment ends: XhoI and NdeI + Fragment size: 5365 + Insert molecule: PCR4704% + Fragment ends: NdeI and XhoI + Fragment size: 368 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + CDS 345..710 + /gene="N6-his-Rrm4-MLLE3" + /SECDrawAs="Gene" + misc_feature 348..365 + /gene="6xHis" + /SECDrawAs="Region" + CDS 366..707 + /gene="Rrm4-M3-679-791AA" + /SECDrawAs="Gene" + misc_feature 368..707 + /gene="MLLE3" + /SECDrawAs="Region" + misc_signal 368..707 + /gene="MLLE3" + /SECDrawAs="Label" + terminator 806..847 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1472..2329 + /gene="bla A - AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4524..5603) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcat catcatcatc + 361 atcacacatt atccacgctt gctgcgcttc ctgcagccga gattgtgcgc ttggccaact + 421 cccaatccag ctccggtctg cctctcccta aagcggatcc agcgacggtc aaggcaaccg + 481 acgatttcat cgactcgctt cagggcaaag cagcgcacga tcagaagcag aagctgggtg + 541 atcagctctt caaaaagatc cgtacgttcg gcgtcaaagg cgcacccaag ctcaccatcc + 601 acttgttgga ttccgaagac ttgcgggcgc tggcgcatct catgaactcg tacgaggacg + 661 tgctcaagga aaaagttcag cacaaggttg ctgcaggtct gaacaagtga ctcgagcacc + 721 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg + 781 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg + 841 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg + 901 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc + 961 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc + 1021 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct + 1081 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac + 1141 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac + 1201 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat + 1261 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa + 1321 aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg gaacccctat + 1381 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata + 1441 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct + 1501 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa + 1561 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa + 1621 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt + 1681 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg + 1741 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca + 1801 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa + 1861 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt + 1921 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc + 1981 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa + 2041 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga + 2101 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc + 2161 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga + 2221 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga + 2281 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga + 2341 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat + 2401 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt + 2461 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct + 2521 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc + 2581 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc + 2641 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc + 2701 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc + 2761 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg + 2821 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata + 2881 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta + 2941 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc + 3001 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg + 3061 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt + 3121 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt + 3181 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga + 3241 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac + 3301 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat + 3361 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc + 3421 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg + 3481 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat + 3541 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac + 3601 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct + 3661 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct + 3721 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc + 3781 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac + 3841 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct + 3901 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc + 3961 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac + 4021 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg + 4081 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc + 4141 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa + 4201 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg + 4261 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc + 4321 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa + 4381 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga + 4441 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac + 4501 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc + 4561 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg + 4621 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga + 4681 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg + 4741 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag + 4801 atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc + 4861 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt + 4921 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga + 4981 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat + 5041 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt + 5101 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca + 5161 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc + 5221 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct + 5281 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga + 5341 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag + 5401 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga + 5461 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg + 5521 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca + 5581 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat + 5641 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc + 5701 cttatgcgac tcctgcatta ggaagcagcc cag +// +LOCUS pUMa4794 5641 bp DNA circular SYN 13-AUG-2024 +DEFINITION Ligation of Insert4794 into VBB - p3262_NE% +ACCESSION pUMa4794 +KEYWORDS . +SOURCE Unknown. + ORGANISM Unknown + Unclassified. +REFERENCE 1 (bases 1 to 5641) + AUTHORS Self + JOURNAL Unpublished. +COMMENT SECID/File created by Clone Manager, Scientific & Educational Software +COMMENT SECNOTES|Vector molecule: VBB - p3262_NE% + Fragment ends: EcoRI and NdeI + Fragment size: 5399 + Insert molecule: Insert4794 + Fragment ends: NdeI and EcoRI + Fragment size: 242 +FEATURES Location/Qualifiers + promoter 257..273 + /gene="T7P" + /product="T7 Promoter" + /SECDrawAs="Region" + misc_signal 276..301 + /gene="Lac O" + /product="Lac operator" + /SECDrawAs="Label" + misc_signal 331..336 + /gene="RBS" + /product="Ribosome binding site" + /SECDrawAs="Label" + CDS 344..578 + /gene="n6His_Pab1-MLLE_567_636AA" + /product="N6xHis_Pa1_ C terminal MLLE" + /SECDrawAs="Gene" + terminator 714..755 + /gene="T7T" + /product="T7 terminator" + /SECDrawAs="Region" + gene 1380..2237 + /gene="bla A - AmpR" + /product="Ampicillin resistant" + /SECDrawAs="Gene" + gene complement (4432..5511) + /gene="LacI" + /product="Laci" + /SECDrawAs="Gene" +ORIGIN + 1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc + 61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat + 121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc + 181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat + 241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc + 301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcat catcatcatc + 361 atcacagtcc cgaggagcag aagcagatgc ttggtgaggc catctacccc aaggttgccg + 421 ccagccagcc cgagcttgcc ggtaagctta ccggtatgat ccttgagctt cccgtcactg + 481 aactcctgca ccttctcgag gagagcgagg cgctggatgc aaaggtcaac gaggctctcg + 541 aggttctcaa ggagtaccaa cagaacgact ctgcgtaagg taccgaattc gagctccgtc + 601 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac + 661 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc + 721 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga + 781 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc + 841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt + 901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag + 961 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt + 1021 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt + 1081 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt + 1141 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt + 1201 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca ggtggcactt + 1261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt + 1321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta + 1381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg + 1441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac + 1501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg + 1561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc + 1621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg + 1681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat + 1741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg + 1801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg + 1861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc + 1921 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt + 1981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct + 2041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc + 2101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca + 2161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct + 2221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt + 2281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga + 2341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca + 2401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac + 2461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg + 2521 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag + 2581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac + 2641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt + 2701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg + 2761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc + 2821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc + 2881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc + 2941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa + 3001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt + 3061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg + 3121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag + 3181 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg + 3241 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta + 3301 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc + 3361 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc + 3421 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc + 3481 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg + 3541 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg + 3601 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta + 3661 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc + 3721 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga + 3781 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt + 3841 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc + 3901 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca + 3961 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa + 4021 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc + 4081 acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg + 4141 ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg + 4201 ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc + 4261 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc + 4321 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga + 4381 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct + 4441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga + 4501 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag + 4561 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg + 4621 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc + 4681 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt + 4741 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac + 4801 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc + 4861 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag + 4921 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc + 4981 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact + 5041 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc + 5101 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg + 5161 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat + 5221 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg + 5281 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc + 5341 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac + 5401 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg + 5461 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac + 5521 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc + 5581 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca + 5641 g +// + + +