assays/S1_A1_Protein[[:space:]]structure[[:space:]]prediction/dataset/Fig01_MLLE3[[:space:]]xray[[:space:]]vs[[:space:]]AF2[[:space:]]vs[[:space:]]Top.pse filter=lfs diff=lfs merge=lfs -text assays/S1_A2_Protein[[:space:]]Purification[[:space:]]for[[:space:]]structural[[:space:]]studies/dataset/Purififed[[:space:]]H-Rrm4-MLLE3[[:space:]](Fig.[[:space:]]S1A).png filter=lfs diff=lfs merge=lfs -text assays/S1_A3_Size[[:space:]]exclusion[[:space:]]chromatography[[:space:]]of[[:space:]]H-Rrm4-MLLE3/dataset/SEC[[:space:]]profile[[:space:]]of[[:space:]]H-Rrm4-MLLE3[[:space:]](p4704)[[:space:]]13122023[[:space:]]skd[[:space:]]v1.xlsx filter=lfs diff=lfs merge=lfs -text assays/S1_A6_X-ray[[:space:]]structure[[:space:]]of[[:space:]]Rrm4-MLLE3/dataset/Rrm4_MLLE3[[:space:]]-[[:space:]]Upa1_PAM2L1.mtz filter=lfs diff=lfs merge=lfs -text assays/S1_A6_X-ray[[:space:]]structure[[:space:]]of[[:space:]]Rrm4-MLLE3/dataset/Rrm4_MLLE3[[:space:]]-[[:space:]]Upa1_PAM2L2.mtz filter=lfs diff=lfs merge=lfs -text assays/S2_A1_Structural[[:space:]]analysis[[:space:]]of[[:space:]]MLLE3[[:space:]]of[[:space:]]Rrm4/dataset/M3[[:space:]]with[[:space:]]PL1,2[[:space:]]v2[[:space:]]skd.pse filter=lfs diff=lfs merge=lfs -text assays/S2_A1_Structural[[:space:]]analysis[[:space:]]of[[:space:]]MLLE3[[:space:]]of[[:space:]]Rrm4/dataset/M3[[:space:]]with[[:space:]]PL1,2.pse filter=lfs diff=lfs merge=lfs -text assays/S2_A4_Structural[[:space:]]comparison[[:space:]]of[[:space:]]MLLE3[[:space:]]in[[:space:]]U_maydis[[:space:]]and[[:space:]]R_irregularis/dataset/RiM3-UmPL2[[:space:]]surface[[:space:]]v1.pse filter=lfs diff=lfs merge=lfs -text assays/S2_A4_Structural[[:space:]]comparison[[:space:]]of[[:space:]]MLLE3[[:space:]]in[[:space:]]U_maydis[[:space:]]and[[:space:]]R_irregularis/dataset/Rrm4_MLLE3[[:space:]]-[[:space:]]Upa1_PAM2L2.pdb filter=lfs diff=lfs merge=lfs -text assays/S2_A5_Western[[:space:]]blot[[:space:]]analyis[[:space:]]of[[:space:]]Rrm4-MLLE3[[:space:]]from[[:space:]]U_maydis[[:space:]]and[[:space:]]R_irregularis/dataset/20231211_Ladder,[[:space:]]BaitP351(PreyP4296,[[:space:]]P4297,P4298),[[:space:]]BaitP0130(PreyP4296,[[:space:]]P4297,P4298)_super-1.tif filter=lfs diff=lfs merge=lfs -text assays/S2_A5_Western[[:space:]]blot[[:space:]]analyis[[:space:]]of[[:space:]]Rrm4-MLLE3[[:space:]]from[[:space:]]U_maydis[[:space:]]and[[:space:]]R_irregularis/dataset/V_20231211_Ladder,[[:space:]]BaitP351(PreyP4296,[[:space:]]P4297,P4298),[[:space:]]BaitP0130(PreyP4296,[[:space:]]P4297,P4298)_super-1.tif filter=lfs diff=lfs merge=lfs -text assays/S4_A2_Purification[[:space:]]of[[:space:]]H-Pab1-MLLE[[:space:]]for[[:space:]]structural[[:space:]]studies/dataset/Fig[[:space:]]S1A,[[:space:]]S3A[[:space:]]SDS[[:space:]]PAGE[[:space:]]of[[:space:]]purified[[:space:]]H-Rrm4-MLLE3,[[:space:]]H-Pab1-MLLE.png filter=lfs diff=lfs merge=lfs -text assays/S4_A3_Size[[:space:]]exclusion[[:space:]]chromotography[[:space:]]of[[:space:]]H-Pab1-MLLE/dataset/Size[[:space:]]exclusion[[:space:]]chromotography[[:space:]]of[[:space:]]H-Pab1-MLLE[[:space:]](P4794).xlsx filter=lfs diff=lfs merge=lfs -text assays/S4_A5_X-ray[[:space:]]structure[[:space:]]analysis[[:space:]]of[[:space:]]H-Pab1-MLLE/dataset/Pab1_MLLE[[:space:]]-[[:space:]]Upa1_PAM2.mtz filter=lfs diff=lfs merge=lfs -text assays/S5_A2_Protein[[:space:]]structure[[:space:]]prediction[[:space:]]using[[:space:]]Alphafold2/dataset/UMAG_15064_Vps8_Alphafold[[:space:]]predicted[[:space:]]model.pdb filter=lfs diff=lfs merge=lfs -text assays/S6_A2_Protein[[:space:]]structure[[:space:]]prediction[[:space:]]of[[:space:]]MKRN1[[:space:]]using[[:space:]]Alphafold2/dataset/Human[[:space:]]and[[:space:]]U.maydis[[:space:]]Mkrn1_Alphafold2[[:space:]]predicted[[:space:]]model.pse filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/Pab1MLLE[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/Rrm4MLLE3[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/V_Pab1MLLE[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/V_Rrm4MLLE3[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/HsPab1_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super4.tif filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/HsUbr5MLLE_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super1.tif filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/V_HsPab1_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super4.tif filter=lfs diff=lfs merge=lfs -text assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/V_HsUbr5MLLE_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super1.tif filter=lfs diff=lfs merge=lfs -text assays/S3_A1_Hyphal[[:space:]]growth[[:space:]]of[[:space:]]strains[[:space:]]expressing[[:space:]]Rrm4-Kat[[:space:]]with[[:space:]]MLLE3[[:space:]]variants[[:space:]]in[[:space:]]AB33[[:space:]]background/dataset/1.[[:space:]]Replicate/UL167/1/BF-DIC.tif filter=lfs diff=lfs merge=lfs -text assays/S3_A1_Hyphal[[:space:]]growth[[:space:]]of[[:space:]]strains[[:space:]]expressing[[:space:]]Rrm4-Kat[[:space:]]with[[:space:]]MLLE3[[:space:]]variants[[:space:]]in[[:space:]]AB33[[:space:]]background/dataset/1.[[:space:]]Replicate/UL167/1/Laser488.tif filter=lfs diff=lfs merge=lfs -text assays/S3_A1_Hyphal[[:space:]]growth[[:space:]]of[[:space:]]strains[[:space:]]expressing[[:space:]]Rrm4-Kat[[:space:]]with[[:space:]]MLLE3[[:space:]]variants[[:space:]]in[[:space:]]AB33[[:space:]]background/dataset/1.[[:space:]]Replicate/UL167/1/X1_Laser488.stk filter=lfs diff=lfs merge=lfs -text assays/S3_A1_Hyphal[[:space:]]growth[[:space:]]of[[:space:]]strains[[:space:]]expressing[[:space:]]Rrm4-Kat[[:space:]]with[[:space:]]MLLE3[[:space:]]variants[[:space:]]in[[:space:]]AB33[[:space:]]background/dataset/1.[[:space:]]Replicate/UL167/1/X2_Laser561.stk filter=lfs diff=lfs merge=lfs -text assays/S3_A1_Hyphal[[:space:]]growth[[:space:]]of[[:space:]]strains[[:space:]]expressing[[:space:]]Rrm4-Kat[[:space:]]with[[:space:]]MLLE3[[:space:]]variants[[:space:]]in[[:space:]]AB33[[:space:]]background/dataset/1.[[:space:]]Replicate/UL167/2/BF-DIC.tif filter=lfs diff=lfs merge=lfs -text assays/S3_A1_Hyphal[[:space:]]growth[[:space:]]of[[:space:]]strains[[:space:]]expressing[[:space:]]Rrm4-Kat[[:space:]]with[[:space:]]MLLE3[[:space:]]variants[[:space:]]in[[:space:]]AB33[[:space:]]background/dataset/1.[[:space:]]Replicate/UL167/2/Laser488.tif filter=lfs diff=lfs merge=lfs -text 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