From 6113331d48b5e8a578d8eba832b0115aa0681f1b Mon Sep 17 00:00:00 2001
From: Sabrina Zander <sabrina.zander@uni-duesseldorf.de>
Date: Tue, 21 Jan 2025 11:25:29 +0100
Subject: [PATCH] add assay 2_1
---
.gitattributes | 8 ++++++++
assays/2-1_Sequencing and Analysis/README.md | 0
.../dataset/.gitkeep | 0
.../RawReads/Background/His-Background.7z | 3 +++
.../RawReads/Background/Strep-Background.7z | 3 +++
.../dataset/RawReads/Heme/DtxR_heme.7z | 3 +++
.../dataset/RawReads/Heme/HrrA_heme.7z | 3 +++
.../dataset/RawReads/Iron/DtxR_Iron.7z | 3 +++
.../dataset/RawReads/Iron/HrrA_Iron.7z | 3 +++
.../RawReads/Starvation/DtxR_Starvation.7z | 3 +++
.../RawReads/Starvation/HrrA_Starvation.7z | 3 +++
.../2-1_Sequencing and Analysis/isa.assay.xlsx | Bin 0 -> 12426 bytes
.../protocols/.gitkeep | 0
.../protocols/Sequencing.txt | 1 +
14 files changed, 33 insertions(+)
create mode 100644 assays/2-1_Sequencing and Analysis/README.md
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/.gitkeep
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Background/His-Background.7z
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Background/Strep-Background.7z
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Heme/DtxR_heme.7z
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Heme/HrrA_heme.7z
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Iron/DtxR_Iron.7z
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Iron/HrrA_Iron.7z
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Starvation/DtxR_Starvation.7z
create mode 100644 assays/2-1_Sequencing and Analysis/dataset/RawReads/Starvation/HrrA_Starvation.7z
create mode 100644 assays/2-1_Sequencing and Analysis/isa.assay.xlsx
create mode 100644 assays/2-1_Sequencing and Analysis/protocols/.gitkeep
create mode 100644 assays/2-1_Sequencing and Analysis/protocols/Sequencing.txt
diff --git a/.gitattributes b/.gitattributes
index 830a6b3..5218a28 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1 +1,9 @@
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+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Starvation/HrrA_Starvation.7z filter=lfs diff=lfs merge=lfs -text
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diff --git a/assays/2-1_Sequencing and Analysis/protocols/.gitkeep b/assays/2-1_Sequencing and Analysis/protocols/.gitkeep
new file mode 100644
index 0000000..e69de29
diff --git a/assays/2-1_Sequencing and Analysis/protocols/Sequencing.txt b/assays/2-1_Sequencing and Analysis/protocols/Sequencing.txt
new file mode 100644
index 0000000..fcbd80c
--- /dev/null
+++ b/assays/2-1_Sequencing and Analysis/protocols/Sequencing.txt
@@ -0,0 +1 @@
+The isolated DNA fragments were used for library preparation and indexing with TruSeq DNA PCR-free sample preparation kit (Illumina, Chesterford, UK) according to manufacturer’s instructions, leaving out the DNA size selection steps. Libraries were quantified using KAPA library quant kit (Peqlab, Bonn, Germany) and normalized for pooling. These pooled libraries were sequenced using the MiSeq device (Illumina) (paired-end sequencing, read length: 2 x 150 bases).
\ No newline at end of file
--
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