diff --git a/studies/3_ReporterStrainGeneration/README.md b/studies/3_ReporterStrainGeneration/README.md new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/3_ReporterStrainGeneration/isa.study.xlsx b/studies/3_ReporterStrainGeneration/isa.study.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..30de2d333f773b722b67751902343157e6b9f8e8 Binary files /dev/null and b/studies/3_ReporterStrainGeneration/isa.study.xlsx differ diff --git a/studies/3_ReporterStrainGeneration/protocols/.gitkeep b/studies/3_ReporterStrainGeneration/protocols/.gitkeep new file mode 100644 index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391 diff --git a/studies/3_ReporterStrainGeneration/protocols/20230210_Cloning_ReporterAssay_DtxRtargets.xlsx b/studies/3_ReporterStrainGeneration/protocols/20230210_Cloning_ReporterAssay_DtxRtargets.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..e2dbd27d4e51a5def9314eab661f0113465d97d7 Binary files /dev/null and b/studies/3_ReporterStrainGeneration/protocols/20230210_Cloning_ReporterAssay_DtxRtargets.xlsx differ diff --git a/studies/3_ReporterStrainGeneration/protocols/20231121_Reporter for DtxR targets.xlsx b/studies/3_ReporterStrainGeneration/protocols/20231121_Reporter for DtxR targets.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..bd2c6212cf20f23e5e683f5452a48881c36134b4 Binary files /dev/null and b/studies/3_ReporterStrainGeneration/protocols/20231121_Reporter for DtxR targets.xlsx differ diff --git a/studies/3_ReporterStrainGeneration/protocols/Strain generation.md b/studies/3_ReporterStrainGeneration/protocols/Strain generation.md new file mode 100644 index 0000000000000000000000000000000000000000..c7f3fa924a5b58def6a4286b0d2c42409a9a5da2 --- /dev/null +++ b/studies/3_ReporterStrainGeneration/protocols/Strain generation.md @@ -0,0 +1,2 @@ +Standard molecular methods were performed according to standard protocols (Sambrook and Russell, 2001). DNA fragments were amplified via polymerase chain reactions (PCR), using chromosomal DNA of C. glutamicum ATCC 13032 as template and the oligonucleotides as listed in metadata. Preparation was performed as described previously (Eikmanns et al., 1994). +Plasmids were constructed by enzymatically assembling the generated DNA fragments into a cut vector backbone using Gibson assembly (Gibson et al., 2009). 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