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+Within the publication, the qPCR only for the ::dtxR-His-Cterm-2link variant is depicted, as the Strep version was not used due to disturbances.
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diff --git a/assays/1-2_qPCR-taggedVariants/dataset/20210304_qPCR_DtxR_Tag_Evaluation.xlsx b/assays/1-2_qPCR-taggedVariants/dataset/20210304_qPCR_DtxR_Tag_Evaluation.xlsx
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diff --git a/assays/1-2_qPCR-taggedVariants/protocols/qPCR_method.txt b/assays/1-2_qPCR-taggedVariants/protocols/qPCR_method.txt
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+For analysis of the C. glutamicum strain with a tagged DtxR variant (::dtxR-C-linker-His), cultivation in deep-well plates (standard, 36 µM FeSO4) or flasks (iron excess, 100 µM FeSO4) was performed, harvesting cells in ice-falcons at an OD600 of ~5 in exponential phase. Using the Luna One-Step RT-qPCR Kit (New England BioLabs, Frankfurt am Main) according to manufacturer’s instructions, qPCR was performed in the qTower (Analytik Jena, Jena). As a reference gene for normalization, the housekeeping gene ddh was used, besides the target gene dtxR. Analysis followed using qPCRsoft 3.1 (Analytik Jena, Jena) and fold-change was calculated according to the 2-ΔΔCt method.
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