From 0fc603bdce99938f1c67c97c457661490aac2315 Mon Sep 17 00:00:00 2001
From: Sabrina Zander <sabrina.zander@uni-duesseldorf.de>
Date: Wed, 3 Jan 2024 12:13:36 +0100
Subject: [PATCH] add protocol to S1_A2

---
 .../protocols/Calcofluor white staining .md                     | 1 +
 assays/S1_A2_microscopy_hyphae/protocols/Growth_condition.md    | 1 +
 assays/S1_A2_microscopy_hyphae/protocols/measurements.md        | 2 ++
 assays/S1_A2_microscopy_hyphae/protocols/microscopy.md          | 1 +
 4 files changed, 5 insertions(+)
 create mode 100644 assays/S1_A2_microscopy_hyphae/protocols/measurements.md

diff --git a/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md b/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md
index 822d477..307e5bd 100644
--- a/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md	
+++ b/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md	
@@ -1 +1,2 @@
+# Calcofluor white staining 
 To visualize basal septa and empty sections, 1 ml of cell culture was stained with 1 μl of Calcofluor white staining solution (2 mg/ml; CFW) directly before microscopy with a DAPI filter set
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diff --git a/assays/S1_A2_microscopy_hyphae/protocols/Growth_condition.md b/assays/S1_A2_microscopy_hyphae/protocols/Growth_condition.md
index 0862226..02885e6 100644
--- a/assays/S1_A2_microscopy_hyphae/protocols/Growth_condition.md
+++ b/assays/S1_A2_microscopy_hyphae/protocols/Growth_condition.md
@@ -1 +1,2 @@
+# Growth conditions
 For microscopic analysis, 20 ml of yeast cells were grown in CM medium (1% glucose) to an OD600 of 0.5 Hyphal cells were induced by shifting 20 ml cell culture from CM medium to NM medium (supplemented either with 1% glucose or 1% arabinose) for 6, 9, 10, and 12 hours. 
\ No newline at end of file
diff --git a/assays/S1_A2_microscopy_hyphae/protocols/measurements.md b/assays/S1_A2_microscopy_hyphae/protocols/measurements.md
new file mode 100644
index 0000000..29b17be
--- /dev/null
+++ b/assays/S1_A2_microscopy_hyphae/protocols/measurements.md
@@ -0,0 +1,2 @@
+# measurements 
+The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.Hyphal cells with empty sections were visualized using Calcofluor White (CFW) and were scored manually. For each experiment, more than 100 cells were counted per strain.
\ No newline at end of file
diff --git a/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md b/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md
index 22985f2..9156ed6 100644
--- a/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md
+++ b/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md
@@ -1,2 +1,3 @@
+# microscopy
 All images were acquired using laser-based epifluorescence-microscopy, Zeiss Axio Observer.Z1 (Oberkochen, Germany) and processed using the MetaMorph software package (version 7.7.0.0, Molecular Devices, Seattle, IL, USA).
 The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.Hyphal cells with empty sections were visualized using Calcofluor White (CFW) and were scored manually. For each experiment, more than 100 cells were counted per strain.
\ No newline at end of file
-- 
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