diff --git a/README.md b/README.md
index dcc23fc21e366fbeb202620d9d5ba6fe38beb408..90edd19b60982e56218a1df067c505d2e736c08e 100644
--- a/README.md
+++ b/README.md
@@ -46,9 +46,9 @@ The [Assays (A)](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tr
 
 [S2_A1_microscopy_Rrm4_Ada_strains_DIC+Rrm4-GFP](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp) Figure S2C-F  
 [S2_A2_protein_expression
-](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A2_protein_expression) Figure S3 A-B  
+](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A2_protein_expression) Figure S3 A  
 [S2_A3_microscopy_Rfp_Gfp
-](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A3_microscopy_Rfp_Gfp) Figure S3 C  
+](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A3_microscopy_Rfp_Gfp) Figure S3 B-C  
 [S2_A4_HyperTRIBE_Rrm4_analysis
 ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A4_HyperTRIBE_Rrm4_analysis) Figure 2C, Figure S4 A-D  
 [S2_A5_HyperTRIBE_Rrm4_iCLIP
diff --git a/assays/S1_A1_microscopy_yeast/README.md b/assays/S1_A1_microscopy_yeast/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..2c05b9bc7bcec62de9bb21f2d9c3542d37e4f404 100644
--- a/assays/S1_A1_microscopy_yeast/README.md
+++ b/assays/S1_A1_microscopy_yeast/README.md
@@ -0,0 +1,2 @@
+# S1_A1_microscopy_yeast
+This assay includes the data of the microscopy experiment of the WT and khd4D yeast strain shown in Figure S1.  
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diff --git a/assays/S1_A2_microscopy_hyphae/README.md b/assays/S1_A2_microscopy_hyphae/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..0a22384f5ede4901daf7d3c8e49e6f570980b3f4 100644
--- a/assays/S1_A2_microscopy_hyphae/README.md
+++ b/assays/S1_A2_microscopy_hyphae/README.md
@@ -0,0 +1,2 @@
+# S1_A2_microscopy_hyphae
+This assay includes microscopy data and quantification of hyphae of the WT and khd4D strain shown in Figure 1.
\ No newline at end of file
diff --git a/assays/S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp/README.md b/assays/S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..877f69636df7fa7bc75052a563a5300a9f99efde 100644
--- a/assays/S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp/README.md
+++ b/assays/S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp/README.md
@@ -0,0 +1,2 @@
+# S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp
+This assay includes microscopy data and quantification of 2805 - pcr1:rrm4-ada-gfp (ip), 2807 - pcrg1:rrm4-gfp (ip), Rrm4-Gfp - prrm4: rrm4-Gfp strains shown in Figure S2C-F.
\ No newline at end of file
diff --git a/assays/S2_A2_protein_expression/README.md b/assays/S2_A2_protein_expression/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..8f0d23501a286a2e26a2ef235f59c8050d3f54ba 100644
--- a/assays/S2_A2_protein_expression/README.md
+++ b/assays/S2_A2_protein_expression/README.md
@@ -0,0 +1,2 @@
+# S2_A2_protein_expression
+This assay includes western blot images of the Rrm4-hyperTRIBE constructs shown in Figure S3 A. 
\ No newline at end of file
diff --git a/assays/S2_A3_microscopy_Rfp_Gfp/README.md b/assays/S2_A3_microscopy_Rfp_Gfp/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..a55ff8a859b3a04ef39982a23f488cfbdd66e624 100644
--- a/assays/S2_A3_microscopy_Rfp_Gfp/README.md
+++ b/assays/S2_A3_microscopy_Rfp_Gfp/README.md
@@ -0,0 +1,2 @@
+# S2_A3_microscopy_Rfp_Gfp
+This assay includes flourescence measurement data and microscopic images of the Rrm4-hyperTRIBE constructs shown in Figure S3 B/C.  
\ No newline at end of file
diff --git a/assays/S2_A4_HyperTRIBE_Rrm4_analysis/README.md b/assays/S2_A4_HyperTRIBE_Rrm4_analysis/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..52547fa746e97f6c138b95d8267f5788d0c9d4d1 100644
--- a/assays/S2_A4_HyperTRIBE_Rrm4_analysis/README.md
+++ b/assays/S2_A4_HyperTRIBE_Rrm4_analysis/README.md
@@ -0,0 +1,2 @@
+# S2_A4_HyperTRIBE_Rrm4_analysis 
+This assay includes RNAseq files (raw/processed) and the script used for the analysis of the Rrm4-Ada-GFP constructs shown in Figure 2C and Figure S4 A-D  
\ No newline at end of file
diff --git a/assays/S2_A5_HyperTRIBE_Rrm4_iCLIP/README.md b/assays/S2_A5_HyperTRIBE_Rrm4_iCLIP/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..0bc05fa0d0890c6307aa42817899677ea2b36200 100644
--- a/assays/S2_A5_HyperTRIBE_Rrm4_iCLIP/README.md
+++ b/assays/S2_A5_HyperTRIBE_Rrm4_iCLIP/README.md
@@ -0,0 +1,2 @@
+# S2_A5_HyperTRIBE_Rrm4_iCLIP
+This assay includes the script used for the analysis shown in Figure 2D and Figure S4 E-G  
diff --git a/assays/S3_A1_microscopy_Khd4-Gfp/README.md b/assays/S3_A1_microscopy_Khd4-Gfp/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..da1505e0088fbbbaccf0e7e4687ce2bee3b8cabd 100644
--- a/assays/S3_A1_microscopy_Khd4-Gfp/README.md
+++ b/assays/S3_A1_microscopy_Khd4-Gfp/README.md
@@ -0,0 +1,2 @@
+# S3_A1_microscopy_Khd4-Gfp 
+This assay includes microscopy data and quantification of WT, khd4D, pcrg1:khd4-ada-gfp, pcrg1:khd4-gfp and pcrg1:mKate2-ada-gfp strain shown in Figure S5 C-E  
\ No newline at end of file
diff --git a/assays/S3_A2_Khd4-Ada-strains_protein_expression/README.md b/assays/S3_A2_Khd4-Ada-strains_protein_expression/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..0a9a4b54105aa62ca93b3eabd4a78d3c5b186c8c 100644
--- a/assays/S3_A2_Khd4-Ada-strains_protein_expression/README.md
+++ b/assays/S3_A2_Khd4-Ada-strains_protein_expression/README.md
@@ -0,0 +1,2 @@
+# S3_A2_Khd4-Ada-strains_protein_expression
+This assay includes includes western blot images of the Khd4-hyperTRIBE constructs shown in Figure S5 F-G 
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