diff --git a/.gitattributes b/.gitattributes
index f9856b14db26d390d2756575254715b4485495c4..f14e9c9fca14397611c35b3e9ed0eab6603aca0b 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -60,3 +60,7 @@ assays/S2_A3_protein_expression/dataset/western_blot_images/UMa2807/GFP/20211203
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 assays/S2_A3_protein_expression/dataset/western_blot_images/UMa2807/actin/20211208_1904.tif filter=lfs diff=lfs merge=lfs -text
 assays/S2_A3_protein_expression/dataset/western_blot_images/UMa2807/actin/V_20211208_1904.tif filter=lfs diff=lfs merge=lfs -text
+assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/GFP/20211203_1549std30sec.tif filter=lfs diff=lfs merge=lfs -text
+assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/GFP/V_20211203_1549std30dsec.tif filter=lfs diff=lfs merge=lfs -text
+assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/actin/20211208_1913.tif filter=lfs diff=lfs merge=lfs -text
+assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/actin/V_20211208_1913.tif filter=lfs diff=lfs merge=lfs -text
diff --git a/assays/S2_A3_protein_expression/dataset/western_blot_images/Readme.txt b/assays/S2_A3_protein_expression/dataset/western_blot_images/Readme.txt
new file mode 100644
index 0000000000000000000000000000000000000000..f76b492e164be18e209622efd1d08c268653832b
--- /dev/null
+++ b/assays/S2_A3_protein_expression/dataset/western_blot_images/Readme.txt
@@ -0,0 +1,7 @@
+UMa2805 (or RAG) - pcrg1:rrm4-ada-gfp (ip)
+UMa2807 (or RG)- pcrg1:rrm4-gfp (ip) 
+UMa3083 (or mkAG) - pcrg1:mKate2-ada-gfp (ip)
+Khd4D - AB33: khd4D
+Wt (or AB33): AB33
+A or Ara or on = Arabinose
+G or Gfp or off = Glucose
diff --git a/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/GFP/20211203_1549std30sec.tif b/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/GFP/20211203_1549std30sec.tif
new file mode 100644
index 0000000000000000000000000000000000000000..0a33b326e0e04f5798c175df848c980461f03602
--- /dev/null
+++ b/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/GFP/20211203_1549std30sec.tif
@@ -0,0 +1,3 @@
+version https://git-lfs.github.com/spec/v1
+oid sha256:2269e2952ba9c5fc26fefb77d2705e6db3d83aa63090563265dba396820ed7f3
+size 1575944
diff --git a/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/GFP/V_20211203_1549std30dsec.tif b/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/GFP/V_20211203_1549std30dsec.tif
new file mode 100644
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diff --git a/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/actin/20211208_1913.tif b/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/actin/20211208_1913.tif
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diff --git a/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/actin/V_20211208_1913.tif b/assays/S2_A3_protein_expression/dataset/western_blot_images/UMa3083/actin/V_20211208_1913.tif
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