diff --git a/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md b/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md new file mode 100644 index 0000000000000000000000000000000000000000..822d477f00b23e589d3a43bb30f9bfbdeac8f5ca --- /dev/null +++ b/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md @@ -0,0 +1 @@ +To visualize basal septa and empty sections, 1 ml of cell culture was stained with 1 μl of Calcofluor white staining solution (2 mg/ml; CFW) directly before microscopy with a DAPI filter set \ No newline at end of file diff --git a/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md b/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md index 60c61f48ccacc67f96aa2c5046127e25e236050c..22985f28899b3ee6836f035fbd1dacad05251098 100644 --- a/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md +++ b/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md @@ -1,2 +1,2 @@ All images were acquired using laser-based epifluorescence-microscopy, Zeiss Axio Observer.Z1 (Oberkochen, Germany) and processed using the MetaMorph software package (version 7.7.0.0, Molecular Devices, Seattle, IL, USA). -The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha. \ No newline at end of file +The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.Hyphal cells with empty sections were visualized using Calcofluor White (CFW) and were scored manually. For each experiment, more than 100 cells were counted per strain. \ No newline at end of file diff --git a/studies/S1_characterization_khd4D/resources/S1_strains.xlsx b/studies/S1_characterization_khd4D/resources/S1_strains.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..fd5dc1655fea1d672469a7740522a96db92711cb Binary files /dev/null and b/studies/S1_characterization_khd4D/resources/S1_strains.xlsx differ