From 4846512f7feb5946904dc3240cef38c32d870dc6 Mon Sep 17 00:00:00 2001 From: Sabrina Zander <sabrina.zander@uni-duesseldorf.de> Date: Wed, 3 Jan 2024 12:02:14 +0100 Subject: [PATCH] add strains S1 --- .../protocols/Calcofluor white staining .md | 1 + .../protocols/microscopy.md | 2 +- .../resources/S1_strains.xlsx | Bin 0 -> 12037 bytes 3 files changed, 2 insertions(+), 1 deletion(-) create mode 100644 assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md create mode 100644 studies/S1_characterization_khd4D/resources/S1_strains.xlsx diff --git a/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md b/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md new file mode 100644 index 0000000..822d477 --- /dev/null +++ b/assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md @@ -0,0 +1 @@ +To visualize basal septa and empty sections, 1 ml of cell culture was stained with 1 μl of Calcofluor white staining solution (2 mg/ml; CFW) directly before microscopy with a DAPI filter set \ No newline at end of file diff --git a/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md b/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md index 60c61f4..22985f2 100644 --- a/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md +++ b/assays/S1_A2_microscopy_hyphae/protocols/microscopy.md @@ -1,2 +1,2 @@ All images were acquired using laser-based epifluorescence-microscopy, Zeiss Axio Observer.Z1 (Oberkochen, Germany) and processed using the MetaMorph software package (version 7.7.0.0, Molecular Devices, Seattle, IL, USA). -The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha. \ No newline at end of file +The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.Hyphal cells with empty sections were visualized using Calcofluor White (CFW) and were scored manually. 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