diff --git a/assays/S5_A2_Kat_protein_level/README.md b/assays/S5_A2_Kat_protein_level/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..8c3601a561d57e074da5b79fa5e2aaf558895bed 100644
--- a/assays/S5_A2_Kat_protein_level/README.md
+++ b/assays/S5_A2_Kat_protein_level/README.md
@@ -0,0 +1,2 @@
+# S5_A2_Kat_protein_level
+This assay include the raw data of the Kat flourescence levels measured in on and off conditions shown in Figure S10C and Figure 4E.  
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diff --git a/assays/S5_A3_Kat_mRNA_level/README.md b/assays/S5_A3_Kat_mRNA_level/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..3a2c75af6b0d3f4ec920c1e1f8db1fc9dc2d6a07 100644
--- a/assays/S5_A3_Kat_mRNA_level/README.md
+++ b/assays/S5_A3_Kat_mRNA_level/README.md
@@ -0,0 +1,2 @@
+# S5_A3_Kat_mRNA_level
+This assay include the mKate2_mRNA expression data shown in Figure 4F  .  
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diff --git a/assays/S6_A1_RNAseq_analysis_yeast_vs_hyphae/README.md b/assays/S6_A1_RNAseq_analysis_yeast_vs_hyphae/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..6e0eb4108ec1cf631bfbd9c94692f8e2b96d80fa 100644
--- a/assays/S6_A1_RNAseq_analysis_yeast_vs_hyphae/README.md
+++ b/assays/S6_A1_RNAseq_analysis_yeast_vs_hyphae/README.md
@@ -0,0 +1,2 @@
+# S6_A1_RNAseq_analysis_yeast_vs_hyphae
+This assay includes script to analyse the differential gene expression and GO-term analysis (yeast versus hyphae) shown in Figure 5 A-C, Figure 6, and Figure S13 A-B.  
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diff --git a/assays/S6_A2_analysis_specific_targets/README.md b/assays/S6_A2_analysis_specific_targets/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..bf12805a7e390610ac964382253e2095d283f3a2 100644
--- a/assays/S6_A2_analysis_specific_targets/README.md
+++ b/assays/S6_A2_analysis_specific_targets/README.md
@@ -0,0 +1,2 @@
+# S6_A2_analysis_specific_targets
+This assay includes the script to analyse specific targets shown in Figure 11 B-D.
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diff --git a/assays/S6_A3_Kat-strains_microscopy/README.md b/assays/S6_A3_Kat-strains_microscopy/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..140fec734aa8e4d379addbb2529ae68cf903e06d 100644
--- a/assays/S6_A3_Kat-strains_microscopy/README.md
+++ b/assays/S6_A3_Kat-strains_microscopy/README.md
@@ -0,0 +1,2 @@
+# S6_A3_Kat-strains_microscopy
+This assay includes microscopic images of the Arl1, Hok1 and Vma21 expression strains shown in Figure 5D, Figure S11 F.
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diff --git a/assays/S6_A3_Kat-strains_microscopy/dataset/README.md b/assays/S6_A3_Kat-strains_microscopy/dataset/README.md
new file mode 100644
index 0000000000000000000000000000000000000000..134cd9c5ce6c716f013dfa629d279c405338a524
--- /dev/null
+++ b/assays/S6_A3_Kat-strains_microscopy/dataset/README.md
@@ -0,0 +1,3 @@
+UL12: AB33 Ptef: hok1-kat_ hok1 3´ UTR / Pcrg1: khd4-gfp /khd4Δ/upp3Δ
+UL8: AB33 Ptef: arl1-kat_ arl1 3´ UTR / Pcrg1: khd4-gfp /khd4Δ/upp3Δ
+UL9: AB33 Ptef: vma21-kat_ vma21 3´ UTR / Pcrg1: khd4-gfp /khd4Δ/upp3Δ
diff --git a/assays/S6_A4_relative_fluorescence_level/README.md b/assays/S6_A4_relative_fluorescence_level/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..f8ab27279194b1551369a785c75de6341e66c653 100644
--- a/assays/S6_A4_relative_fluorescence_level/README.md
+++ b/assays/S6_A4_relative_fluorescence_level/README.md
@@ -0,0 +1,2 @@
+# S6_A4_relative_fluorescence_level
+This assay shows the flourescence signal data and analysis of the Arl1, Hok1 and Vma21 expression strains shown in Figure S11 G.  
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diff --git a/assays/S8_A1_microscopy_FM4-64/README.md b/assays/S8_A1_microscopy_FM4-64/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..7b7a45e2b5323e0428b75d3d30067cb555c0ee3f 100644
--- a/assays/S8_A1_microscopy_FM4-64/README.md
+++ b/assays/S8_A1_microscopy_FM4-64/README.md
@@ -0,0 +1,2 @@
+# S8_A1_microscopy_FM4-64
+This assay includes microscopic images and quantification data of WT, did2D and khd4D strains, stained with FM4-64 shown in Figure S13 C-D.   
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diff --git a/assays/S8_A2_microscopy_CMAG/README.md b/assays/S8_A2_microscopy_CMAG/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..7366e1e3775feff5e6c0939b021aa3f6e3aa6a0d 100644
--- a/assays/S8_A2_microscopy_CMAG/README.md
+++ b/assays/S8_A2_microscopy_CMAG/README.md
@@ -0,0 +1,2 @@
+# S8_A2_microscopy_CMAG
+This assay includes microscopic images and quantification data of WT, did2D and khd4D strains, stained with CMAG shown in Figure 7 .  
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