diff --git a/assays/S3_A3_HyperTRIBE_Khd4_workflow/README.md b/assays/S3_A3_HyperTRIBE_Khd4_workflow/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..3210ebbf38f6ce4b8ed9702d84a86f6724f19349 100644
--- a/assays/S3_A3_HyperTRIBE_Khd4_workflow/README.md
+++ b/assays/S3_A3_HyperTRIBE_Khd4_workflow/README.md
@@ -0,0 +1,2 @@
+# S3_A3_HyperTRIBE_Khd4_workflow
+This assay includes the RNAseq files (raw/processed) and scripts used for the analysis of the Kdh4-Ada-GFP constructs shown in Figure 3A, Figure S6A.  
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diff --git a/assays/S3_A4_HyperTRIBE_motif_analysis/README.md b/assays/S3_A4_HyperTRIBE_motif_analysis/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..ecc5cd52fc8672824c13b26202284bfec87fcbd4 100644
--- a/assays/S3_A4_HyperTRIBE_motif_analysis/README.md
+++ b/assays/S3_A4_HyperTRIBE_motif_analysis/README.md
@@ -0,0 +1,2 @@
+# S3_A3_HyperTRIBE_motif_analysis
+This assay includes the scripts used for the motif analysis of the Kdh4-Ada-GFP constructs shown in Figure S6 B-E, Figure 3, Figure S7, Figure S8 A-D  
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diff --git a/assays/S3_A5_GO-term/README.md b/assays/S3_A5_GO-term/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..c75ed5595f109816643fedbe804d138162913bc2 100644
--- a/assays/S3_A5_GO-term/README.md
+++ b/assays/S3_A5_GO-term/README.md
@@ -0,0 +1,2 @@
+# S3_A5_GO-term
+This assay includes the scripts used for the GO-term analysis of the Kdh4-Ada-GFP constructs shown S8 E, Figure S9  
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diff --git a/assays/S4_A1_RNAseq_data/README.md b/assays/S4_A1_RNAseq_data/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..ebd3e4a53edcfa73a9b75ce70f66881668172f0a 100644
--- a/assays/S4_A1_RNAseq_data/README.md
+++ b/assays/S4_A1_RNAseq_data/README.md
@@ -0,0 +1,2 @@
+# S4_A1_RNAseq_data
+This assay includes RNAseq data (raw / processed ) for  khd4D and WT yeast and hyphae shown in Figure 4 A-C, Fig S10A   
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diff --git a/assays/S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae/README.md b/assays/S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..943bca0f610b99cce532f7f1af4bf89f5437f7fb 100644
--- a/assays/S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae/README.md
+++ b/assays/S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae/README.md
@@ -0,0 +1,2 @@
+# S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae
+This assay includes the script for RNAseq analysis for khd4D and WT yeast and hyphae shown in Figure 4 A-C, Fig S10A . 
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diff --git a/assays/S5_A1_microscopy_khd4-targets/README.md b/assays/S5_A1_microscopy_khd4-targets/README.md
index e69de29bb2d1d6434b8b29ae775ad8c2e48c5391..066358331355449a2dc92a7d99e9d89c64d0ecb5 100644
--- a/assays/S5_A1_microscopy_khd4-targets/README.md
+++ b/assays/S5_A1_microscopy_khd4-targets/README.md
@@ -0,0 +1,2 @@
+# S5_A1_microscopy_khd4-targets 
+This assay includes microscopic images of the differnt motife strains shown in Figure S10B.
diff --git a/assays/S5_A1_microscopy_khd4-targets/dataset/README.md b/assays/S5_A1_microscopy_khd4-targets/dataset/README.md
new file mode 100644
index 0000000000000000000000000000000000000000..0ca45be290e067a09d120d6389474fe945aba6d6
--- /dev/null
+++ b/assays/S5_A1_microscopy_khd4-targets/dataset/README.md
@@ -0,0 +1,5 @@
+3139: AB33 Ptef: kat_no motif / Pcrg1: khd4-gfp / khd4Δ / upp3Δ
+3140: AB33 Ptef: kat_(auaccc)6 / Pcrg1: khd4-gfp / khd4Δ / upp3Δ
+3141: AB33 Ptef: kat_(agaucu)6 / Pcrg1: khd4-gfp / khd4Δ / upp3Δ
+3238: AB33 Ptef: kat_(auaccc) / Pcrg1: khd4-gfp /khd4Δ/upp3Δ
+3239: AB33 Ptef: kat_(agaucu) / Pcrg1: khd4-gfp /khd4Δ/upp3Δ
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