diff --git a/assays/BSA_RNAseq/README.md b/assays/BSA_RNAseq/README.md
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/BSA_RNAseq/dataset/.gitkeep b/assays/BSA_RNAseq/dataset/.gitkeep
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/BSA_RNAseq/isa.assay.xlsx b/assays/BSA_RNAseq/isa.assay.xlsx
new file mode 100644
index 0000000000000000000000000000000000000000..9324508ba83d8fc7de131265ca9544c4ed46c2a6
Binary files /dev/null and b/assays/BSA_RNAseq/isa.assay.xlsx differ
diff --git a/assays/BSA_RNAseq/protocols/.gitkeep b/assays/BSA_RNAseq/protocols/.gitkeep
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/BSA_WGS/README.md b/assays/BSA_WGS/README.md
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/BSA_WGS/dataset/.gitkeep b/assays/BSA_WGS/dataset/.gitkeep
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/BSA_WGS/isa.assay.xlsx b/assays/BSA_WGS/isa.assay.xlsx
new file mode 100644
index 0000000000000000000000000000000000000000..b0d04c36800363cbf1fea9691799092554c508ab
Binary files /dev/null and b/assays/BSA_WGS/isa.assay.xlsx differ
diff --git a/assays/BSA_WGS/protocols/.gitkeep b/assays/BSA_WGS/protocols/.gitkeep
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/isoform_sequencing/README.md b/assays/isoform_sequencing/README.md
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/isoform_sequencing/dataset/.gitkeep b/assays/isoform_sequencing/dataset/.gitkeep
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/assays/isoform_sequencing/isa.assay.xlsx b/assays/isoform_sequencing/isa.assay.xlsx
new file mode 100644
index 0000000000000000000000000000000000000000..f433b98075131124d47d729f0b2c1a8c21bd73cc
Binary files /dev/null and b/assays/isoform_sequencing/isa.assay.xlsx differ
diff --git a/assays/isoform_sequencing/protocols/.gitkeep b/assays/isoform_sequencing/protocols/.gitkeep
new file mode 100644
index 0000000000000000000000000000000000000000..e69de29bb2d1d6434b8b29ae775ad8c2e48c5391
diff --git a/isa.investigation.xlsx b/isa.investigation.xlsx
index 560da1b18ead7fec5475a603b73fa29e9f78b5be..8dd9edaaa0cc7b479255b792b3d6a51a9694f494 100644
Binary files a/isa.investigation.xlsx and b/isa.investigation.xlsx differ
diff --git a/studies/Bulk_segregant_analysis/isa.study.xlsx b/studies/Bulk_segregant_analysis/isa.study.xlsx
index f648f53de39af67005ad073d4ed2ed4520da3616..fa958df03fd9c7b9b754ad5f26d51436acc31872 100644
Binary files a/studies/Bulk_segregant_analysis/isa.study.xlsx and b/studies/Bulk_segregant_analysis/isa.study.xlsx differ
diff --git a/studies/isoform_sequencing/isa.study.xlsx b/studies/isoform_sequencing/isa.study.xlsx
index 28123c3f96ce7b158be275401e00901d529ed28f..7d3d989efaa92c2afc24de856111c7eba732e34f 100644
Binary files a/studies/isoform_sequencing/isa.study.xlsx and b/studies/isoform_sequencing/isa.study.xlsx differ