RNA seq data: - big diffrence in reads per sample (50 mil to 250mil) -> low number better is not problem but strange - most genes observed (apx. 24_000 of 30_000) - Noise of data high - HKX not 0, only down regulated - PCA with arrows show interesting patterns matched by ANOVA hits analysis - Go Enrichment shows interesting results Meatboloimics: - For NPS HXK has increase in T0 which dissapear in eCO2 Kinetics: - HPR has inccreased HXK rates compared to other genotypes - Col reches HPR levels of HPR under eCO2 - Glutamine Sythase incresed in HPR - HPR has less SPS activity in aCO2 - HPR activity basicaly is 0 in HPR Glutamine in HXK mutatnt? ## TODO: Corretlation of Glutamate in Mitochondria both in BOU and in WT to see where Carbon of Glutamte goes to in BOU HXk: - Increase in phophaste - In context of HXK - related to metabolites -> Pyruvate in Cytosol - related to stress responce - more stressed in high aCO2 - Diffrences in PC1 vs PC2 HPR: - Clear convincing storry - Is MLS necessary BOU: - How is Glutamate correlated to BOU - Correlation to other metabolites - String Correlation with BOU for possible interaactions - regulatory role though phosphorilation site - pathway that consumes Glutamate off in aCO2